A 96-well format for a high-throughput baculovirus generation, fast titering and recombinant protein production in insect and mammalian cells

<p>Abstract</p> <p>Background</p> <p>Baculovirus expression vector system (BEVS) has become a standard in recombinant protein production and virus-like particle preparation for numerous applications.</p> <p>Findings</p> <p>We describe here protocols which adapt baculovirus generation into 96-well format.</p> <p>Conclusion</p> <p>The established methodology allows simple baculovirus generation, fast virus titering within 18 h and efficient recombinant protein production in a high-throughput format. Furthermore, the produced baculovirus vectors are compatible with gene expression in vertebrate cells <it>in vitro </it>and <it>in vivo</it>.</p

Havaintoja venäjän kielen lukupuhunnan sävelkulun ja spontaanin puheen jaksottelun oppimisesta

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