Deltex E3 Ubiquitin Ligase 3L confers radioresistance in prostate cancer via Akt pathway

Purpose: To determine the effect of Deltex E3 Ubiquitin Ligase 3L (DTX3L) on the radioresistance of prostate cancer (PCa).Methods: A PCa cell model of radioresistance was established via exposure of cancer cell lines to fractionated radiation. The MTT {(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)} assay and western blotting were performed to evaluate the impact of DTX3L on cell survival and DNA damage repair. The molecular mechanism of action was evaluated by western blotting.Results: DTX3L was elevated in PCa cell lines compared with normal primary prostate epithelial cells (p < 0.01). The survival of PCa cells exposed to radiation was promoted by overexpression of DTX3L, while knockdown of DTX3L abrogated the radioresistance. Moreover, overexpression of DTX3Ldecreased phosphorylation of histone H2AX (γH2AX) and increased Rad51 levels (p < 0.01). However, knockdown of DTX3L reversed the accumulation of γH2AX and Rad51. Phosphorylation of AKT was promoted by DTX3L overexpression, but was reduced by DTX3L knockdown (p < 0.01). Inhibition of AKT (protein kinase B) counteracted the promotion ability of DTX3L on the radioresistance of PCa cells via decreased cell survival ratio, and also inhibited DNA damage repair via accumulation of γ-H2AX and depletion of Rad51 (p < 0.01).Conclusion: DTX3L increases the resistance of prostate cancer to radiotherapy and DNA damage repair in PCa via AKT pathway, indicating a potential therapeutic strategy to overcome radioresistance in PCa. Keywords: DTX3L (Deltex E3 Ubiquitin Ligase 3L), DNA damage, Phosphorylation, Radioresistance, AKT, Protein kinase B, Prostate cance

Leveraging ChatGPT for Power System Programming Tasks

The rapid digitalization of power systems has led to a significant increase in coding tasks for power engineers. This research article explores how ChatGPT, an advanced AI language model, can assist power engineers and researchers in a range of coding tasks. From simple to complex, we present three case studies to illustrate the benefits of ChatGPT in various coding scenarios. For routine tasks such as daily unit commitment, ChatGPT can increase efficiency by directly generating batch number of codes and reducing repetitive programming and debugging time for power engineers. For complex problems such as decentralized optimization of mul-ti-vector energy systems, ChatGPT can reduce the learning cost of power engineers on problem formulation and the choice of numerical solvers. For new problems without readily avaliable solutions such as ultra-fast unit commitment, ChatGPT can organize technology roadmap, gen-erate data and develop model and code. Furthermore, this paper discuss generic prompt ap-proaches for different tasks in power systems, providing insights for power engineers and re-searchers seeking to harness ChatGPT in terms of auto coding, new knowledge learning and new problem solving. The findings demonstrate the potential of ChatGPT as a powerful tool in the domain of power system engineering

Evaluation of Juglans regia L., root for wound healing via antioxidant, antimicrobial and anti-inflammatory activity

304-311The burden of the management of problematic skin wounds characterized by a compromised skin barrier is growing rapidly. There is an urgent requirement for efficient mechanism-based treatments and more efficacious drug delivery systems. The present study was aimed to examine the wound healing potential of Juglans regia L. root (JR) in rats by incision and excision wound methods via the anti-inflammatory, antioxidant and antimicrobial activities. We have used tensile strength and biochemical parameters for studying the wound healing properties of JR by incision wound methodology. The anti-inflammatory effect was assessed by the measurement of paw edema in carrageenan-induced inflammation in rats. The wound contraction area, antioxidant status, and antimicrobial studies were exhausted excision wound methodology. There was a significant decrease in percent inhibition of paw edema (0.63 ± 0.03 to 0.33 ± 0.02 after 24 h) with an increase in JR concentration. Tensile strength and hydroxyproline level of different concentrations (1, 2.5, 5, and 10% w/w) of JR ointment treated groups were found significantly (P <0.001) comparable to the reference group. Moreover, JR showed significant antimicrobial and antioxidant properties, by its ability to increase antioxidant and antimicrobial levels. In conclusion, the overall results obtained in this study clarify that JR inhibits paw edema and accelerates cutaneous wound healing

The apparent focal depth, emergence angle, and take-off angle of seismic wave measured by YRY-4-type borehole strainmeter as one kind of strain seismograph

Introduction: In theory, the observation objects and principles of strain seismograph and traditional pendulum seismograph are different, and the characteristics of observed signals should also be dissimilar. The observation results of pendulum seismograph show that seismic waves in inhomogeneous media will undergo refraction, reflection, and attenuation. Then, what signal characteristics can be detected by strain seismograph is great significance for understanding and explaining the observation results.Methods: Using YRY-4 type four-gauge borehole strainmeter as one kind of strain seismograph to detect the strain tensor change of the plane seismic wave emitted from the surface, a five-site strain seismograph observation network was built in Shanxi Province, with continuous observation for 2 years at a sampling rate of 100 Hz. In this paper, two local events occurring in the area covered by the strain seismograph observation network are taken as examples. We systematically studied the characteristics of seismic wave signals recorded by strain seismographs at five sites, inverted for the focal depth of the two local earthquakes and the relationship between the wave velocity and the wave velocity gradient of the focal depth, and calculated the apparent focal depth, the emergence angle and the take-off angle of seismic waves.Results: These results show stable uniqueness and apparent regularity, especially since the inverted focal depths are basically consistent with the seismic solutions based on those traditional pendulum seismographs. The observations from this study show that the strain seismograph can be used as an effective supplement to the pendulum seismograph.Discussion: In the future, we will continue to study the rupture process and focal mechanism of moderate-strong earthquakes and teleseismic earthquakes by combining two kinds of observations

NEW EFFECTIVE TRANSFORMATIONAL COMPUTATIONAL METHODS

Mathematics serves as a fundamental intelligent theoretic basis for computation, and mathematical analysis is very useful to develop computational methods to solve various problems in science and engineering. Integral transforms such as Laplace Transform have been playing an important role in computational methods. In this paper, we will introduce Sumudu Transform in a new computational approach, in which effective computational methods will be developed and implemented. Such computational methods are straightforward to understand, but powerful to incorporate into computational science to solve different problems automatically. We will provide computational analysis and essentiality by surveying and summarizing some related recent works, with additional automatic proof details by applying system built-in functions. Applications include the computation of coefficients of Taylor\u27s expansions, calculation of generating functions, mathematical identity proofs, solving differential equations and integral equations. For demonstration purposes, some of the methods were implemented in Maple with demonstrational results matching the expected values

Shyness Weakens the Agreeableness-Prosociality Association via Social Self-Efficacy: A Moderated-Mediation Study of Chinese Undergraduates

Using a sample of 1383 undergraduate students (Mage = 20.06, Nfemale = 817), this study tested a moderated mediation model in which shyness moderated the association between agreeableness and prosocial behavior, as well as the relation between agreeableness and social self-efficacy (SSE). Results showed (when gender, age, and family socio-economic status were controlled) that agreeableness exerted a positive effect on prosocial behavior (PSB) toward three types of recipients (i.e., family members, friends/acquaintances, strangers), and this effect was mediated by SSE and moderated by shyness. The relationships between agreeableness and PSB were more positive under low shyness than that under high shyness condition. In addition, shyness also moderated the first stage of mediation model (i.e., the agreeableness-SSE association), showing that the relation between agreeableness and SSE was more positive under low shyness than that under high shyness condition. Identifying the moderation effect of shyness provides evidence that personality traits may operate in an interactive manner. This may shed new light on why there are inconsistent findings regarding the agreeableness-prosociality association

FGF: A web tool for Fishing Gene Family in a whole genome database

Gene duplication is an important process in evolution. The availability of genome sequences of a number of organisms has made it possible to conduct comprehensive searches for duplicated genes enabling informative studies of their evolution. We have established the FGF (Fishing Gene Family) program to efficiently search for and identify gene families. The FGF output displays the results as visual phylogenetic trees including information on gene structure, chromosome position, duplication fate and selective pressure. It is particularly useful to identify pseudogenes and detect changes in gene structure. FGF is freely available on a web server at http://fgf.genomics.org.cn

Virtual inertia control to active support of the variable-speed wind turbine in variable frequency limit time

Constraining the virtual inertia of variable-speed wind turbines within the variable frequency limit time will be the key to improving the wind power’s reliability in actively supporting the system frequency. First, this paper analyzes the controllable characteristics and evaluation methods of the virtual inertia of the wind turbine. Second, it quantifies the variable frequency limit time of the grid with high-proportion wind power and considers the wind turbine speed and power margin to restrict the virtual inertia of the wind turbine during the period of frequency drop or increase to provide reliable inertia support to ensure the safety of the system frequency. On this basis, the inertia demand under the frequency safety of the system was analyzed, and the virtual inertia control strategy of the variable-speed wind turbine in the variable frequency limit time was proposed using the speed tracking performance of the wind turbine. Finally, the grid with a high-proportion wind power simulation system is built to verify that the variable-speed wind turbine can reliably satisfy the inertia demand of system frequency modulation under the proposed control strategy and significantly improve its active support performance to the power grid