SPOC online video learning clustering analysis: Identifying learners' group behavior characteristics

With the widespread of Small Private Online Courses (SPOC) in colleges and universities, course organizers who provide high-quality personalized course activities need to understand learners' learning status and characteristics, and then optimize the course teaching. However, little research has been done on different learners' group behavior characteristics, such as which indicators can reflect learner groups' behavior, and what are the typical behavior characteristics of different learner groups. In this work, we established a Python Language Foundation self-built SPOC course, and 109 undergraduates' learning behavior data were collected and analyzed. From 74-dimensional behavior log data consisting of 72 video-viewing, course video score, and comprehensive score, Principal Component Analysis was performed to reduce dimensionality. Agglomerative hierarchical clustering was used to divide learners into different categories, and the results showed that 15 groups are clustered. According to the analysis of the four indicators for group characteristics, which are the completion and viewing-stability of task-point videos, unit test excellence, and comprehensive score, it is identified into five primary types, including positive type, regular type, negative type, semi-negative type, and a fluctuation type. Experiments conducted on a real data set across different academic years and courses show that the proposed approach has better clustering accuracy and practicability. It is expected that this work may be used to strengthen the personalized learning support services system in educational institutions and develop a tool that integrates exploration and analysis work onto the web platform

Experimental study on grouting reinforcement characteristics of limestone with different length cracks under fluid solid coupling

It is difficult to fully develop coal resources in the north of the Yellow River due to the influence of mine water disaster. Grouting technology is an important means to solve the problem of mine water disasters at present. The in-depth study of grouting technology can not only liberate a large number of coal resources threatened by water disasters, but also have positive significance for the protection of groundwater resources. Laboratory tests were conducted on limestone samples obtained from Qiuji Coal Mine in the Yellow River North Coalfield to observe the microscopic characteristics of the limestone. The mechanical properties and grouting reinforcement characteristics of limestone with different lengths of cracks under fluid solid coupling were obtained. Through theoretical analysis, the variation law of the cracking strength of limestone with the change of prefabricated crack length was obtained, and the grouting reinforcement mechanism was analyzed. The test results show that the strength and toughness of the rock after grouting have been significantly improved, the limestone before grouting has obvious brittleness characteristics, and the brittleness of the limestone after grouting has decreased and the ductility has increased; The damage degree of grouting plus solid is more and more severe with the increase of crack length. When the crack length is large, it is accompanied by horizontal fracture development; The pre-peak permeability of grouting plus solid decreases slightly, and the post-peak permeability decreases significantly. The maximum stage of limestone permeability occurs in the post-peak stage, while the minimum stage of permeability occurs in the linear elastic stage of the stress-strain curve. The longer the fracture length, the easier the double-peak phenomenon of permeability appears. Based on the theory of continuous medium mechanics, the formulas for calculating the crack initiation strength and fracture toughness of grouting plus solid are derived. It is theoretically proved that the strength of grouting plus solid is greater than that of the original rock sample; The greater the difference between the elastic modulus of rock and the elastic modulus of the combination formed by grouting, the weaker the impact of grouting on rock strength

Label-free microfluidic paper-based electrochemical aptasensor for ultrasensitive and simultaneous multiplexed detection of cancer biomarkers

Simultaneous detection of multiple tumor biomarkers in body fluids could facilitate early diagnosis of lung cancer, so as to provide scientific reference for clinical treatment. This paper depicted a multi-parameter paper-based electrochemical aptasensor for simultaneous detection of carcinoembryonic antigen (CEA) and neuron-specific enolase (NSE) in a clinical sample with high sensitivity and specificity. The paper-based device was fabricated through wax printing and screen-printing, which enabled functions of sample filtration and sample auto injection. Amino functional graphene (NG)-Thionin (THI)- gold nanoparticles (AuNPs) and Prussian blue (PB)- poly (3,4- ethylenedioxythiophene) (PEDOT)- AuNPs nanocomposites were synthesized respectively. They were used to modify the working electrodes not only for promoting the electron transfer rate, but also for immobilization of the CEA and NSE aptamers. A label-free electrochemical method was adopted, enabling a rapid simple point-of-care testing. Experimental results showed that the proposed multi-parameter aptasensor exhibited good linearity in ranges of 0.01-500 ng mL for CEA (R  = 0.989) and 0.05-500 ng mL for NSE (R  = 0.944), respectively. The limit of detection (LOD) was 2 pg mL for CEA and 10 pg mL for NSE. In addition, the device was evaluated using clinical serum samples and received a good correlation with large electrochemical luminescence (ECL) equipment, which would offer a new platform for early cancer diagnostics, especially in those resource-limit areas

Propamidine decreas mitochondrial complex III activity of Botrytis cinerea

Propamidine, an aromatic diamidine compound, is widely used as an antimicrobial agent. To uncover its mechanism on pathogenetic fungi, Botrytis cinerea as an object was used to investigate effects of propamidine in this paper. The transmission electron microscope results showed that the mitochondrial membranes were collapsed after propamidine treatment, followed that mitochondria were disrupted. Inhibition of whole-cell and mitochondrial respiration by propamidine suggested that Propamidine is most likely an inhibitor of electron transport within Botrytis cinerea mitochondria. Furthermore, the mitochondrial complex III activity were inhibited by propamidine. [BMB reports 2010; 43(9): 614-621

Synthesis of Icariin-Zinc and its Protective Effect on Exercise Fatigue and Reproductive System Related Glands in Male Rats

Background: Icariin, a traditional Chinese medicine, plays a protective role in the treatment of exercise fatigue. Zinc, a trace element, plays an important role in the reproductive system. Therefore, we aimed to synthesize an Icariin-Zinc complex (by chemical means) and verify its protective effect on exercise fatigue and the reproductive system using animal experiments.Methods: The icariin-zinc complex was prepared by the reaction of icariin carbonyl and zinc ions (molar ratio 1:3). The molecular formula and structural formula of the complex were identified and tested. Fifty-six rats selected by swimming training were randomly divided into six groups: static control, exercise control, icariin, gluconate zinc (G-Zn group), icariin glucose zinc and icariin-zinc exercise ( low, high dose/L-E group, H-E group) groups. These groups respectively received the following doses: 1 ml/100 g, daily gavage with NS (for the first two groups), 45 mg/kg icariin, 110 mg/kg Gluconate Zinc, Icariin glucose zinc (45 mg/kg Icariin and 110 mg/kg Gluconate Zinc), 60 mg/kg icariin zinc and 180 mg/kg icariin zinc. After 3 weeks of gavage, we conducted 6 weeks of exhaustive swimming training. Test indices such as exhaustive swimming time of rats and body weight were evaluated after the last training exercise. The seminal vesicles, testes, and prostate gland were weighed, and their indices were calculated. The levels of testosterone (in the plasma) and glycogen (in the liver and muscle homogenates) were also evaluated using ELISA.Results: Compared with the static control group, the exhaustive swimming time of the rats in each group was prolonged. Compared with the other groups, the exhaustive swimming time of the L-E and H-E groups was significantly longer (p < 0.01); the Icariin-Zinc complex significantly increased the exhaustive swimming time of the rats. Compared with the static control group, the plasma testosterone content of the L-E and H-E groups increased significantly (p < 0.05). Compared with the exercise control group and G-Zn group, the plasma testosterone content of the H-E group also increased significantly (p < 0.01). The Icariin-Zinc complex significantly increased the serum levels of testosterone in rats. Compared with the control group, the muscle glycogen reserves of each group decreased, indicating that the muscle glycogen reserves of the rats decreased after swimming. Compared with other groups, the Icariin-Zinc complex can reduce the level of glycogen in the muscles, indicating that it can increase the utilization efficiency of glycogen in muscles. Compared with the static control and exercise control groups, the testicular weight of rats in the administration groups increased slightly. The Icariin-Zinc complex increased the testicular weight, indicating that the function of the reproductive system was improved to some extent.Conclusion: Icariin-Zinc can significantly prolong the exhaustive swimming time, improve exercise ability, and increase the plasma testosterone level (which is beneficial for improving the reproductive ability of male rats). Moreover, the beneficial effect of Icariin-Zinc on the glycogen content, testis index, and other reproductive system glands is dose-dependent

Photosynthesis responses of endemic shrubs of Taklimakan Desert to adverse temperature, humidity and radiation

Under the native habitat conditions, the seasonal gas exchange characteristics of two natural endemic plant species, Calligonum taklimakanensis B.R. Pan & GM. Shen and Tamarix taklamakanensis M.T. Liu, which are located in the hinterland of the Taklimakan Desert, are measured and compared by Li-6400 photosynthesis system. The results indicate that temperature (degrees C), solar radiation (PAR), soil water content (SWC), and other environmental factors have obvious seasonal variations and the gas exchange characteristics of two plants have different changes in different growing seasons. For C. taklimakanensis, both in July and September, its daily changes of net photosynthetic rate tend to be obvious double peak curve, but in July its peak appeared earlier. Besides its maximum net photosynthetic rate (P-max), apparent quantum efficiency (Phi), range of effective photosynthetic radiation significantly less than that in September. Moreover, its water use efficiency (WUE) in July was also lower than that in September due to the higher transpiration rate (T-r). For T. taklamakanensis, although its daily change of net photosynthetic rate is a single peak curve in September, its peak time has not changed, and except that its WUE is higher in September like C. taklimakanensis, the maximum net photosynthetic rate (P-max), apparent quantum efficiency (Phi), light saturation point, and range of effective photosynthetic radiation has not changed or slightly declined. That is to say C. taklimakanensis select a season that habitat was better (like September) to progress relative effectively photosynthesis accumulation, in contrast, T. taklamakanensis still keep a relatively stable photosynthesis rate in different growth seasons. The difference of gas exchange characteristics of the two plants in different seasons shows that adaptation strategies of the two plants to extreme conditions in desert are different. Besides, both the higher photosynthetic accumulation rate and the higher water use efficiency in September also indicate that these two endemic desert shrubs possess the abilities and strategies to make the best of limited natural resources

Amide proton transfer-weighted magnetic resonance imaging for the differentiation of parotid gland tumors

PurposeTo assess the usefulness of amide proton transfer-weighted (APTw) imaging in the differentiation of parotid gland tumors.Materials and methodsPatients with parotid gland tumors who underwent APTw imaging were retrospectively enrolled and divided into groups according to pathology. Two radiologists evaluated the APTw image quality independently, and APTw images with quality score ≥3 were enrolled. The maximum and average values of APTw imaging for tumor lesions (APTmax and APTmean) were measured. The differences in APTmax and APTmean were compared between malignant tumors (MTs) and benign tumors (BTs), as well as between MTs and pleomorphic adenomas (PAs) and between MTs and Warthin tumors (WTs). Independent-samples t-test, Kruskal–Wallis H test, and receiver operating characteristic (ROC) curve analyses were used for statistical analysis.ResultsSeventy-three patients were included for image quality evaluation. In this study, 32/73 and 29/73 parotid tumors were scored as 4 and 3, respectively. After excluding lesions with quality score ≤2 (12/73), the APTmean and APTmax of MTs were 4.15% ± 1.33% and 7.43% ± 1.61%, higher than those of BTs 2.74% ± 1.04% and 5.25% ± 1.54%, respectively (p < 0.05). The areas under the ROC curve (AUCs) of the APTmean and APTmax for differentiation between MTs and BTs were 0.819 and 0.821, respectively. MTs indicated significantly higher APTmean and APTmax values than those of PAs (p < 0.05) and WTs (p < 0.05). The AUCs of the APTmean and APTmax for differentiation between MTs and PAs were 0.830 and 0.815 and between MTs and WTs were 0.847 and 0.920, respectively.ConclusionMost APTw images for parotid tumors had acceptable image quality for APTw value evaluation. Both APTmax and APTmean can be used to differentiate MTs from BTs and to differentiate MTs from subtype parotid gland tumors

Characterizing corn-straw-degrading actinomycetes and evaluating application efficiency in straw-returning experiments

Corn straw is an abundant lignocellulose resource and by-product of agricultural production. With the continuous increase in agricultural development, the output of corn straw is also increasing significantly. However, the inappropriate disposal of straw results in wasting of resources, and also causes a serious ecological crisis. Screening microorganisms with the capacity to degrade straw and understanding their mechanism of action is an efficient approach to solve such problems. For this purpose, our research group isolated three actinomycete strains with efficient lignocellulose degradation ability from soil in the cold region of China: Streptomyces sp. G1T, Streptomyces sp. G2T and Streptomyces sp. G3T. Their microbial properties and taxonomic status were assessed to improve our understanding of these strains. The three strains showed typical characteristics of the genus Streptomyces, and likely represent three different species. Genome functional annotation indicated that most of their genes were related to functions like carbohydrate transport and metabolism. In addition, a similar phenomenon also appeared in the COG and CAZyme analyses, with a large number of genes encoding carbohydrate-related hydrolases, such as cellulase, glycosidase and endoglucanase, which could effectively destroy the structure of lignocellulose in corn straw. This unambiguously demonstrated the potential of the three microorganisms to hydrolyze macromolecular polysaccharides at the molecular level. In addition, in the straw-returning test, the decomposing consortium composed of the three Streptomyces isolates (G123) effectively destroyed the recalcitrant bonds between the various components of straw, and significantly reduced the content of active components in corn straw. Furthermore, microbial diversity analysis indicated that the relative abundance of Proteobacteria, reportedly associated with soil antibiotic resistance and antibiotic degradation, was significantly improved with straw returning at both tested time points. The microbial diversity of each treatment was also dramatically changed by supplementing with G123. Taken together, G123 has important biological potential and should be further studied, which will provide new insights and strategies for appropriate treatment of corn straw

Nomenclatural and taxonomic notes on Rubus davidianus Kuntze and R. viburnifolius Franch

Critical examinations of specimens, with literature reviews, have shown that Rubus davidianus is conspecific with R. lambertianus. Therefore, we treat R. davidianus as a new synonym within Rubus. We propose a new name, Rubus loirensis Ti R. Huang nom. nov. to replace the later homonym of R. pycnanthus Genev. Additionally, lectotypification of three names, R. davidianus Kuntze, R. malifolius Focke and R. viburnifolius Franch., are designated here after examination of previous works