Assessment of Biphasic Calcium Phosphate mixed with injectable platelet rich fibrin (i-PRF) on healing of surgically created bone defects in a sheep animal model (A Histological Analysis)

Aims: Assessment of Biphasic Calcium Phosphate mixed with injectable platelet-rich fibrin (i-PRF) on healing of surgically created bone defects in a sheep animal model. Materials and Methods: In each tibia / radius of five sheep, three defects each measuring 7mm in diameter and 4mm in depth were created. The defects were filled with study materials and in the following order: from a proximal to distal orientation; the first defect was filled with biphasic calcium phosphate alone, the second left empty to be filled by physiological clot, and the third with i-PRF mixed with biphasic calcium phosphate. Histological examination of bone defects was made to assess bone formation at fourtime intervals (two, four, six and eight weeks) post-surgically. Results: Regarding bone formation, histological findings showed the presence of a significant difference within the time intervals in the BCP+i-PRF group and in the BCP group when compared with the control group with the highest mean being at eight weeks post-surgery in the BCP+i-PRF group. Conclusions: Both BCP and i-PRF, enhanced bone formation when compared to the control group and throughout the period of study and as disclosed by histological findings

Impact of Biphasic Calcium Phosphate mixed with injectable platelet rich fibrin (i-PRF) on bone density in surgically created bone defects in a sheep animal model (A Radiographic Assessment)

Aims: To assess bone density means with Biphasic calcium phosphate alone or mixed with injectable platelet-rich fibrin in bone defects created surgically in sheep’s tibia / radius using standard radiographic assessment. Materials and Methods: Three bony defects of 4mm full and 7 mm in diameter were created in every tibias/radius of five sheep. The defects were stuffed within the following order: from a proximal to distal orientation; 1st defect was full of biphasic calcium phosphate alone, the second was left empty to be filled by physiological clot and also the third with i-PRF mixed with BCP and covered with absorbable collagen membrane for tissue guiding and then sutured. Densitometric analysis of bone defects was made to measure density of bone at four-time intervals (two, four, six and eight weeks) post-surgically. Results: Densitometric analysis results showed a major distinction within the BCP+i-PRF group and BCP group compared with the control one and at all-time intervals with the best density being in the 1st group followed by the BCP alone group and least in the control group. Conclusions: BCP and i-PRF, both of them increased and sustained bone density (synergistic effect) throughout the study period as revealed by densitometric data