Cold water upwelling and entrainment near the Anadyr Strait: Implications to the North Pacific-Arctic interaction

The Tenth Symposium on Polar Science/Ordinary sessions: [OM] Polar Meteorology and Glaciology, Wed. 4 Dec. / Entrance Hall (1st floor) , National Institute of Polar Researc

Fixed-Point Observation of Mixed Layer Evolution in the Seasonally Ice-Free Chukchi Sea: Turbulent Mixing due to Gale Winds and Internal Gravity Waves

第6回極域科学シンポジウム[OM] 極域気水圏11月16日（月）　国立極地研究所１階交流アトリウ

Do Strong Winds Impact Water Mass, Nutrient, and Phytoplankton Distributions in the Ice‐Free Canada Basin in the Fall?

In general, strong wind events can enhance ocean turbulent mixing, followed by episodic nutrient supply to the euphotic zone and phytoplankton blooms. However, it is unclear whether such responses to strong winds occur in the ice‐free Canada Basin, where the seasonal pycnocline is strong and the nutricline is deep. In the present study, we monitored a fixed‐point observation (FPO) station in the Canada Basin for about 3 weeks in the fall of 2014 to examine the oceanic and biological responses to strong winds. At the FPO site, oceanic microstructure measurements, hydrographic surveys, and water sampling were performed with high temporal resolution, recording internal wave propagation, eddy passage, and water mass changes. Strong winds and internal wave propagation significantly enhanced the mixing above and at the seasonal pycnocline, but their effects were diminished at the nutricline, which was much deeper than the seasonal pycnocline. Therefore, wind‐induced mixing did not increase the upward nutrient supply from the nutricline and did not impact phytoplankton (chlorophyll a) distribution in the surface layer of the FPO site. The temporal evolution of the chlorophyll a concentration was most closely related to water mass changes. We also observed prominent subsurface chlorophyll a maxima with abundant large‐sized phytoplankton that were likely carried by warm‐core eddies to the FPO site. Phytoplankton biomass may have been sustained by the high concentration of ammonium within the eddy and ammonium regeneration at the seasonal pycnocline, where particulate organic matter likely accumulated

Cardiac magnetic resonance imaging-based myocardial strain study for evaluation of cardiotoxicity in breast cancer patients treated with trastuzumab: A pilot study to evaluate the feasibility of the method

Background: Trastuzumab, used to treat breast cancer overexpressing human epidermal growth factor receptor 2, may be cardiotoxic. Cardiac magnetic resonance (CMR) imaging with myocardial strain studies has been used to evaluate subclinical biventricular myocardial changes, however, its clinical utility during chemotherapy has not been evaluated. Methods: The clinical outcomes, CMR and cardiac biomarkers of 9 women aged 62.3 ± 12.6 years with early or locally advanced breast cancer were evaluated at baseline, and at 3, 6 and 12 months after the initiation of trastuzumab. Results: None of the patients developed heart failure or elevated serum cardiac biomarkers. Global left ventricular (LV) peak systolic longitudinal and circumferential strains were significantly decreased at 6 months (longitudinal strains, –21.1 ± 1.7% [baseline] vs. –19.5 ± 1.0% [6 months], p = 0.039, and circumferential strains, –23.4 ± 1.8% [baseline] vs. –21.6 ± 2.5% [6 months], p = 0.036). These changes were analogous to those observed in the LV ejection fraction. Right ventricular (RV) free wall peak systolic circumferential strains were decreased at 6 months (–20.9% ± 2.4% [baseline] vs. –19.1% ± 2.3% [6 months], p = 0.049), whereas RV longitudinal strains and ejection fraction remained unchanged. The LV longitudinal strain was the most reproducible of the 4 peak strain parameters. Conclusions: The LV longitudinal and circumferential strains measured by CMR decreased during trastuzumab therapy, although their predictive value for later heart failure or association with RV parameters was not determined. These techniques may be a useful means of diagnosing and monitoring trastuzumab-related cardiotoxicity

3,6-Diphenyltetrazine as Cathode Active Material for Sodium Ion Batteries

3, 6-diphenyltetrazine (DPT) is an electron-deficient π-conjugated molecule with a perfectly planar structure and high crystallinity. In this study, discharge-charge tests of crystalline DPT as a cathode material for sodium ion batteries were conducted. DPT showed an initial reversible capacity of 102 mAh/g (theoretical capacity 114 mAh/g), corresponding to one electron reaction. The plateau of the discharge-charge profiles was observed at 1.9–2.1 V vs. Na/Na⁺. According to the ex-situ XRD, FT-IR, and XPS measurements to investigate the discharge-charge mechanism, the redox center was identified as the conjugated tetrazine ring. DPT was in a crystalline form in both the charged and discharged state and indicated the potential as a reversible Na ion host

A Method for Similarity Search of Genomic Positional Expression Using CAGE

With the advancement of genome research, it is becoming clear that genes are not distributed on the genome in random order. Clusters of genes distributed at localized genome positions have been reported in several eukaryotes. Various correlations have been observed between the expressions of genes in adjacent or nearby positions along the chromosomes depending on tissue type and developmental stage. Moreover, in several cases, their transcripts, which control epigenetic transcription via processes such as transcriptional interference and genomic imprinting, occur in clusters. It is reasonable that genomic regions that have similar mechanisms show similar expression patterns and that the characteristics of expression in the same genomic regions differ depending on tissue type and developmental stage. In this study, we analyzed gene expression patterns using the cap analysis gene expression (CAGE) method for exploring systematic views of the mouse transcriptome. Counting the number of mapped CAGE tags for fixed-length regions allowed us to determine genomic expression levels. These expression levels were normalized, quantified, and converted into four types of descriptors, allowing the expression patterns along the genome to be represented by character strings. We analyzed them using dynamic programming in the same manner as for sequence analysis. We have developed a novel algorithm that provides a novel view of the genome from the perspective of genomic positional expression. In a similarity search of expression patterns across chromosomes and tissues, we found regions that had clusters of genes that showed expression patterns similar to each other depending on tissue type. Our results suggest the possibility that the regions that have sense–antisense transcription show similar expression patterns between forward and reverse strands

Association of TNFAIP3 interacting protein 1, TNIP1 with systemic lupus erythematosus in a Japanese population: a case-control association study

INTRODUCTION: TNFAIP3 interacting protein 1, TNIP1 (ABIN-1) is involved in inhibition of nuclear factor-κB (NF-κB) activation by interacting with TNF alpha-induced protein 3, A20 (TNFAIP3), an established susceptibility gene to systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Recent genome-wide association studies revealed association of TNIP1 with SLE in the Caucasian and Chinese populations. In this study, we investigated whether the association of TNIP1 with SLE was replicated in a Japanese population. In addition, association of TNIP1 with RA was also examined. METHODS: A case-control association study was conducted on the TNIP1 single nucleotide polymorphism (SNP) rs7708392 in 364 Japanese SLE patients, 553 RA patients and 513 healthy controls. RESULTS: Association of TNIP1 rs7708392C was replicated in Japanese SLE (allele frequency in SLE: 76.5%, control: 69.9%, P = 0.0022, odds ratio [OR] 1.40, 95% confidence interval [CI] 1.13-1.74). Notably, the risk allele frequency in the healthy controls was considerably greater in Japanese (69.9%) than in Caucasians (24.3%). A tendency of stronger association was observed in the SLE patients with renal disorder (P = 0.00065, OR 1.60 [95%CI 1.22-2.10]) than in all SLE patients (P = 0.0022, OR 1.40 [95%CI 1.13-1.74]). Significant association with RA was not observed, regardless of the carriage of human leukocyte antigen DR β1 (HLA-DRB1) shared epitope. Significant gene-gene interaction between TNIP1 and TNFAIP3 was detected neither in SLE nor RA. CONCLUSIONS: Association of TNIP1 with SLE was confirmed in a Japanese population. TNIP1 is a shared SLE susceptibility gene in the Caucasian and Asian populations, but the genetic contribution appeared to be greater in the Japanese and Chinese populations because of the higher risk allele frequency. Taken together with the association of TNFAIP3, these observations underscore the crucial role of NF-κB regulation in the pathogenesis of SLE

PRX2 and PRX25, peroxidases regulated by COG1, are involved in seed longevity in Arabidopsis

[EN] Permeability is a crucial trait that affects seed longevity and is regulated by different polymers including proanthocyanidins, suberin, cutin and lignin located in the seed coat. By testing mutants in suberin transport and biosynthesis, we demonstrate the importance of this biopolymer to cope with seed deterioration. Transcriptomic analysis of cog1-2D, a gain-of-function mutant with increased seed longevity, revealed the upregulation of several peroxidase genes. Reverse genetics analysing seed longevity uncovered redundancy within the seed coat peroxidase gene family; however, after controlled deterioration treatment, seeds from the prx2 prx25 double and prx2 prx25 prx71 triple mutant plants presented lower germination than wild-type plants. Transmission electron microscopy analysis of the seed coat of these mutants showed a thinner palisade layer, but no changes were observed in proanthocyanidin accumulation or in the cuticle layer. Spectrophotometric quantification of acetyl bromide-soluble lignin components indicated changes in the amount of total polyphenolics derived from suberin and/or lignin in the mutant seeds. Finally, the increased seed coat permeability to tetrazolium salts observed in the prx2 prx25 and prx2 prx25 prx71 mutant lines suggested that the lower permeability of the seed coats caused by altered polyphenolics is likely to be the main reason explaining their reduced seed longevityRenard, J.; Martínez-Almonacid, I.; Sonntag, A.; Molina, I.; Moya-Cuevas, J.; Bissoli, G.; Muñoz-Bertomeu, J.... (2020). PRX2 and PRX25, peroxidases regulated by COG1, are involved in seed longevity in Arabidopsis. Plant Cell & Environment. 43(2):315-326. https://doi.org/10.1111/pce.13656S315326432Almagro, L., Gómez Ros, L. V., Belchi-Navarro, S., Bru, R., Ros Barceló, A., & Pedreño, M. A. (2008). Class III peroxidases in plant defence reactions. Journal of Experimental Botany, 60(2), 377-390. doi:10.1093/jxb/ern277Bailly, C., El-Maarouf-Bouteau, H., & Corbineau, F. (2008). From intracellular signaling networks to cell death: the dual role of reactive oxygen species in seed physiology. Comptes Rendus Biologies, 331(10), 806-814. doi:10.1016/j.crvi.2008.07.022Beisson, F., Li, Y., Bonaventure, G., Pollard, M., & Ohlrogge, J. B. (2007). The Acyltransferase GPAT5 Is Required for the Synthesis of Suberin in Seed Coat and Root of Arabidopsis. The Plant Cell, 19(1), 351-368. doi:10.1105/tpc.106.048033Belmonte, M. F., Kirkbride, R. C., Stone, S. L., Pelletier, J. M., Bui, A. Q., Yeung, E. C., … Harada, J. J. (2013). Comprehensive developmental profiles of gene activity in regions and subregions of the Arabidopsis seed. Proceedings of the National Academy of Sciences, 110(5), E435-E444. doi:10.1073/pnas.1222061110Bernards, M. A. (2002). Demystifying suberin. Canadian Journal of Botany, 80(3), 227-240. doi:10.1139/b02-017Bernards, M. A., Summerhurst, D. K., & Razem, F. A. (2004). Oxidases, peroxidases and hydrogen peroxide: The suberin connection. Phytochemistry Reviews, 3(1-2), 113-126. doi:10.1023/b:phyt.0000047810.10706.46Bolger, A. M., Lohse, M., & Usadel, B. (2014). Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics, 30(15), 2114-2120. doi:10.1093/bioinformatics/btu170Bueso, E., Muñoz-Bertomeu, J., Campos, F., Brunaud, V., Martínez, L., Sayas, E., … Serrano, R. (2013). ARABIDOPSIS THALIANA HOMEOBOX25 Uncovers a Role for Gibberellins in Seed Longevity. Plant Physiology, 164(2), 999-1010. doi:10.1104/pp.113.232223Châtelain, E., Satour, P., Laugier, E., Ly Vu, B., Payet, N., Rey, P., & Montrichard, F. (2013). Evidence for participation of the methionine sulfoxide reductase repair system in plant seed longevity. Proceedings of the National Academy of Sciences, 110(9), 3633-3638. doi:10.1073/pnas.1220589110Clerkx, E. J. M., Blankestijn-De Vries, H., Ruys, G. J., Groot, S. P. C., & Koornneef, M. (2004). Genetic differences in seed longevity of various Arabidopsis mutants. Physiologia Plantarum, 121(3), 448-461. doi:10.1111/j.0031-9317.2004.00339.xCosio, C., & Dunand, C. (2009). Specific functions of individual class III peroxidase genes. Journal of Experimental Botany, 60(2), 391-408. doi:10.1093/jxb/ern318Czechowski, T., Stitt, M., Altmann, T., Udvardi, M. K., & Scheible, W.-R. (2005). Genome-Wide Identification and Testing of Superior Reference Genes for Transcript Normalization in Arabidopsis. Plant Physiology, 139(1), 5-17. doi:10.1104/pp.105.063743Debeaujon, I., Léon-Kloosterziel, K. M., & Koornneef, M. (2000). Influence of the Testa on Seed Dormancy, Germination, and Longevity in Arabidopsis. Plant Physiology, 122(2), 403-414. doi:10.1104/pp.122.2.403Duroux, L., & Welinder, K. G. (2003). The Peroxidase Gene Family in Plants: A Phylogenetic Overview. Journal of Molecular Evolution, 57(4), 397-407. doi:10.1007/s00239-003-2489-3Fedi, F., O’Neill, C. M., Menard, G., Trick, M., Dechirico, S., Corbineau, F., … Penfield, S. (2017). Awake1, an ABC-Type Transporter, Reveals an Essential Role for Suberin in the Control of Seed Dormancy. Plant Physiology, 174(1), 276-283. doi:10.1104/pp.16.01556Francoz, E., Ranocha, P., Nguyen-Kim, H., Jamet, E., Burlat, V., & Dunand, C. (2015). Roles of cell wall peroxidases in plant development. Phytochemistry, 112, 15-21. doi:10.1016/j.phytochem.2014.07.020Franke, R., Briesen, I., Wojciechowski, T., Faust, A., Yephremov, A., Nawrath, C., & Schreiber, L. (2005). Apoplastic polyesters in Arabidopsis surface tissues – A typical suberin and a particular cutin. Phytochemistry, 66(22), 2643-2658. doi:10.1016/j.phytochem.2005.09.027Franke, R., & Schreiber, L. (2007). Suberin — a biopolyester forming apoplastic plant interfaces. Current Opinion in Plant Biology, 10(3), 252-259. doi:10.1016/j.pbi.2007.04.004GoffL TrapnellC&KelleyD(2014)CummeRbund: Analysis exploration manipulation and visualization of Cufflinks high‐throughput sequencing data. R package version 2.22.0.Gou, M., Hou, G., Yang, H., Zhang, X., Cai, Y., Kai, G., & Liu, C.-J. (2016). The MYB107 Transcription Factor Positively Regulates Suberin Biosynthesis. Plant Physiology, 173(2), 1045-1058. doi:10.1104/pp.16.01614Graça, J. (2015). Suberin: the biopolyester at the frontier of plants. Frontiers in Chemistry, 3. doi:10.3389/fchem.2015.00062Haughn, G., & Chaudhury, A. (2005). Genetic analysis of seed coat development in Arabidopsis. Trends in Plant Science, 10(10), 472-477. doi:10.1016/j.tplants.2005.08.005Herrero, J., Fernández-Pérez, F., Yebra, T., Novo-Uzal, E., Pomar, F., Pedreño, M. Á., … Zapata, J. M. (2013). Bioinformatic and functional characterization of the basic peroxidase 72 from Arabidopsis thaliana involved in lignin biosynthesis. Planta, 237(6), 1599-1612. doi:10.1007/s00425-013-1865-5Kim, D., Langmead, B., & Salzberg, S. L. (2015). HISAT: a fast spliced aligner with low memory requirements. Nature Methods, 12(4), 357-360. doi:10.1038/nmeth.3317Kosma, D. K., Murmu, J., Razeq, F. M., Santos, P., Bourgault, R., Molina, I., & Rowland, O. (2014). At MYB 41 activates ectopic suberin synthesis and assembly in multiple plant species and cell types. The Plant Journal, 80(2), 216-229. doi:10.1111/tpj.12624Kunieda, T., Shimada, T., Kondo, M., Nishimura, M., Nishitani, K., & Hara-Nishimura, I. (2013). Spatiotemporal Secretion of PEROXIDASE36 Is Required for Seed Coat Mucilage Extrusion in Arabidopsis  . The Plant Cell, 25(4), 1355-1367. doi:10.1105/tpc.113.110072Lee, Y., Rubio, M. C., Alassimone, J., & Geldner, N. (2013). A Mechanism for Localized Lignin Deposition in the Endodermis. Cell, 153(2), 402-412. doi:10.1016/j.cell.2013.02.045Liang, M., Davis, E., Gardner, D., Cai, X., & Wu, Y. (2006). Involvement of AtLAC15 in lignin synthesis in seeds and in root elongation of Arabidopsis. Planta, 224(5), 1185-1196. doi:10.1007/s00425-006-0300-6Li-Beisson, Y., Shorrosh, B., Beisson, F., Andersson, M. X., Arondel, V., Bates, P. D., … Ohlrogge, J. (2013). Acyl-Lipid Metabolism. The Arabidopsis Book, 11, e0161. doi:10.1199/tab.0161Mandel, T., Candela, H., Landau, U., Asis, L., Zilinger, E., Carles, C. C., & Williams, L. E. (2016). Differential regulation of meristem size, morphology and organization by the ERECTA, CLAVATA and class III HD-ZIP pathways. Development. doi:10.1242/dev.129973Milne, I., Stephen, G., Bayer, M., Cock, P. J. A., Pritchard, L., Cardle, L., … Marshall, D. (2012). Using Tablet for visual exploration of second-generation sequencing data. Briefings in Bioinformatics, 14(2), 193-202. doi:10.1093/bib/bbs012Molina, I., Bonaventure, G., Ohlrogge, J., & Pollard, M. (2006). The lipid polyester composition of Arabidopsis thaliana and Brassica napus seeds. Phytochemistry, 67(23), 2597-2610. doi:10.1016/j.phytochem.2006.09.011Molina, I., Ohlrogge, J. B., & Pollard, M. (2007). Deposition and localization of lipid polyester in developing seeds of Brassica napus and Arabidopsis thaliana. The Plant Journal, 53(3), 437-449. doi:10.1111/j.1365-313x.2007.03348.xMoreira‐Vilar F C. Siqueira‐Soares R deC Finger‐Teixeira A. Oliveira de D. M. Ferro AP Rocha daG J. Ferrarese M deLL Santos dosW. D. Ferrarese‐Filho O(2014).The Acetyl Bromide Method Is Faster Simpler and Presents Best Recovery of Lignin in Different Herbaceous Tissues than Klason and Thioglycolic Acid Methods. PLoS ONE 9:e110000.https://doi.org/10.1371/journal.pone.0110000Oñate-Sánchez, L., & Vicente-Carbajosa, J. (2008). DNA-free RNA isolation protocols for Arabidopsis thaliana, including seeds and siliques. BMC Research Notes, 1(1), 93. doi:10.1186/1756-0500-1-93Østergaard, L., Teilum, K., Mirza, O., Mattsson, O., Petersen, M., Welinder, K. G., … Henriksen, A. (2000). Plant Molecular Biology, 44(2), 231-243. doi:10.1023/a:1006442618860Pfaffl, M. W. (2001). A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research, 29(9), 45e-45. doi:10.1093/nar/29.9.e45Passardi, F., Longet, D., Penel, C., & Dunand, C. (2004). The class III peroxidase multigenic family in rice and its evolution in land plants☆☆☆. Phytochemistry, 65(13), 1879-1893. doi:10.1016/j.phytochem.2004.06.023Pedreira, J., Herrera, M. T., Zarra, I., & Revilla, G. (2010). The overexpression of AtPrx37, an apoplastic peroxidase, reduces growth in Arabidopsis. Physiologia Plantarum, 141(2), 177-187. doi:10.1111/j.1399-3054.2010.01427.xPollard, M., Beisson, F., Li, Y., & Ohlrogge, J. B. (2008). Building lipid barriers: biosynthesis of cutin and suberin. Trends in Plant Science, 13(5), 236-246. doi:10.1016/j.tplants.2008.03.003Quiroga, M., Guerrero, C., Botella, M. A., Barceló, A., Amaya, I., Medina, M. I., … Valpuesta, V. (2000). A Tomato Peroxidase Involved in the Synthesis of Lignin and Suberin. Plant Physiology, 122(4), 1119-1128. doi:10.1104/pp.122.4.1119Rains, M. K., Gardiyehewa de Silva, N. D., & Molina, I. (2017). Reconstructing the suberin pathway in poplar by chemical and transcriptomic analysis of bark tissues. Tree Physiology, 38(3), 340-361. doi:10.1093/treephys/tpx060Russell, W. R., Burkitt, M. J., Scobbie, L., & Chesson, A. (2005). EPR Investigation into the Effects of Substrate Structure on Peroxidase-Catalyzed Phenylpropanoid Oxidation. Biomacromolecules, 7(1), 268-273. doi:10.1021/bm050636oSano, N., Rajjou, L., North, H. M., Debeaujon, I., Marion-Poll, A., & Seo, M. (2015). Staying Alive: Molecular Aspects of Seed Longevity. Plant and Cell Physiology, 57(4), 660-674. doi:10.1093/pcp/pcv186Shigeto, J., Itoh, Y., Hirao, S., Ohira, K., Fujita, K., & Tsutsumi, Y. (2015). Simultaneously disrupting AtPrx2 , AtPrx25 and AtPrx71 alters lignin content and structure in Arabidopsis stem. Journal of Integrative Plant Biology, 57(4), 349-356. doi:10.1111/jipb.12334Shigeto, J., Kiyonaga, Y., Fujita, K., Kondo, R., & Tsutsumi, Y. (2013). Putative Cationic Cell-Wall-Bound Peroxidase Homologues in Arabidopsis, AtPrx2, AtPrx25, and AtPrx71, Are Involved in Lignification. Journal of Agricultural and Food Chemistry, 61(16), 3781-3788. doi:10.1021/jf400426gSoliday, C. L., Dean, B. B., & Kolattukudy, P. E. (1978). Suberization: Inhibition by Washing and Stimulation by Abscisic Acid in Potato Disks and Tissue Culture. Plant Physiology, 61(2), 170-174. doi:10.1104/pp.61.2.170Tobimatsu, Y., Chen, F., Nakashima, J., Escamilla-Trevino, L. L., Jackson, L., Dixon, R. A., & Ralph, J. (2013). Coexistence but Independent Biosynthesis of Catechyl and Guaiacyl/Syringyl Lignin Polymers in Seed Coats. The Plant Cell, 25(7), 2587-2600. doi:10.1105/tpc.113.113142Trapnell, C., Hendrickson, D. G., Sauvageau, M., Goff, L., Rinn, J. L., & Pachter, L. (2012). Differential analysis of gene regulation at transcript resolution with RNA-seq. Nature Biotechnology, 31(1), 46-53. doi:10.1038/nbt.2450Vishwanath, S. J., Delude, C., Domergue, F., & Rowland, O. (2014). Suberin: biosynthesis, regulation, and polymer assembly of a protective extracellular barrier. Plant Cell Reports, 34(4), 573-586. doi:10.1007/s00299-014-1727-zVishwanath, S. J., Kosma, D. K., Pulsifer, I. P., Scandola, S., Pascal, S., Joubès, J., … Domergue, F. (2013). Suberin-Associated Fatty Alcohols in Arabidopsis: Distributions in Roots and Contributions to Seed Coat Barrier Properties  . Plant Physiology, 163(3), 1118-1132. doi:10.1104/pp.113.224410Vogt, T. (2010). Phenylpropanoid Biosynthesis. Molecular Plant, 3(1), 2-20. doi:10.1093/mp/ssp106Wang, G.-L., Que, F., Xu, Z.-S., Wang, F., & Xiong, A.-S. (2016). Exogenous gibberellin enhances secondary xylem development and lignification in carrot taproot. Protoplasma, 254(2), 839-848. doi:10.1007/s00709-016-0995-6Yadav, V., Molina, I., Ranathunge, K., Castillo, I. Q., Rothstein, S. J., & Reed, J. W. (2014). ABCG Transporters Are Required for Suberin and Pollen Wall Extracellular Barriers in Arabidopsis    . The Plant Cell, 26(9), 3569-3588. doi:10.1105/tpc.114.129049Zieslin, N., & Ben-Zaken, R. (1992). Effects of applied auxin, gibberellin and cytokinin on the activity of peroxidases in the peduncles of rose flowers. Plant Growth Regulation, 11(1), 53-57. doi:10.1007/bf0002443