Fluorescence visualization of the molecular assembly processes during solvent evaporation via aggregation-induced emission in a cyanostilbene derivative

The initial stage of organic crystal formation during solvent evaporation was observed using aggregation-induced enhanced emission, indicating that the fluorescence from the J-aggregates was established prior to the increase in total intensity at the assembly process. This suggests that the kinetics of the transformation of J-aggregates into embryonic nuclei, and subsequently into crystals, can be visualized using fluorescence.ArticleCRYSTENGCOMM. 16(42):9779-9782 (2014)journal articl

Fluorescence Enhancement of a Dicyanostilbene Derivative Film Casted from an Alcoholic Solution Triggered by UV-light Irradiation

The films of a dicyanostilbene derivative (DBDCS) cast from an alcoholic solution show aggregation-induced emission enhancement by UV light irradiation and a fluorescence color change from green to blue upon heating. The former can be attributed to the planarization by rotational motion around the olefin moieties due to elevation in the local temperature via the excited state by UV irradiation. The latter originates from the phase transformation of DBDCS with cooperative alcoholic desorption and molecular reorganization.ArticleChemistry Letters.45(4):421-423(2016)journal articl

Age-dependent motor dysfunction due to neuron-specific disruption of stress-activated protein kinase MKK7.

c-Jun N-terminal kinase (JNK) is a member of the mitogen-activated protein kinase family and controls various physiological processes including apoptosis. A specific upstream activator of JNKs is the mitogen-activated protein kinase kinase 7 (MKK7). It has been reported that MKK7-JNK signaling plays an important regulatory role in neural development, however, post-developmental functions in the nervous system have not been elucidated. In this study, we generated neuron-specific Mkk7 knockout mice (MKK7 cKO), which impaired constitutive activation of JNK in the nervous system. MKK7 cKO mice displayed impaired circadian behavioral rhythms and decreased locomotor activity. MKK7 cKO mice at 8 months showed motor dysfunctions such as weakness of hind-limb and gait abnormality in an age-dependent manner. Axonal degeneration in the spinal cord and muscle atrophy were also observed, along with accumulation of the axonal transport proteins JNK-interacting protein 1 and amyloid beta precursor protein in the brains and spinal cords of MKK7 cKO mice. Thus, the MKK7-JNK signaling pathway plays important roles in regulating circadian rhythms and neuronal maintenance in the adult nervous system

FGF2 Has Distinct Molecular Functions from GDNF in the Mouse Germline Niche

Both glial cell line-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (FGF2) are bona fide self-renewal factors for spermatogonial stem cells, whereas retinoic acid (RA) induces spermatogonial differentiation. In this study, we investigated the functional differences between FGF2 and GDNF in the germline niche by providing these factors using a drug delivery system in vivo. Although both factors expanded the GFRA1+ subset of undifferentiated spermatogonia, the FGF2-expanded subset expressed RARG, which is indispensable for proper differentiation, 1.9-fold more frequently than the GDNF-expanded subset, demonstrating that FGF2 expands a differentiation-prone subset in the testis. Moreover, FGF2 acted on the germline niche to suppress RA metabolism and GDNF production, suggesting that FGF2 modifies germline niche functions to be more appropriate for spermatogonial differentiation. These results suggest that FGF2 contributes to induction of differentiation rather than maintenance of undifferentiated spermatogonia, indicating reconsideration of the role of FGF2 in the germline niche

A Genome-wide Microsatellite Polymorphism Database for the Indica and Japonica Rice

Microsatellite (MS) polymorphism is an important source of genetic diversity, providing support for map-based cloning and molecular breeding. We have developed a new database that contains 52 845 polymorphic MS loci between indica and japonica, composed of ample Class II MS markers, and integrated 18 828 MS loci from IRGSP and genetic markers from RGP. Based on genetic marker positions on the rice genome (http://rise.genomics.org.cn/rice2/index.jsp ), we determined the approximate genetic distances of these MS loci and validated 100 randomly selected markers experimentally with 90% success rate. In addition, we recorded polymorphic MS positions in indica cv. 9311 that is the most important paternal parent of the two-line hybrid rice in China. Our database will undoubtedly facilitate the application of MS markers in genetic researches and marker-assisted breeding. The data set is freely available from www.wigs.zju.edu.cn/achievment/polySSR

Trans-omics Impact of Thymoproteasome in Cortical Thymic Epithelial Cells

The thymic function to produce self-protective and self-tolerant T cells is chiefly mediated by cortical thymic epithelial cells (cTECs) and medullary TECs (mTECs). Recent studies including single-cell transcriptomic analyses have highlighted a rich diversity in functional mTEC subpopulations. Because of their limited cellularity, however, the biochemical characterization of TECs, including the proteomic profiling of cTECs and mTECs, has remained unestablished. Utilizing genetically modified mice that carry enlarged but functional thymuses, here we show a combination of proteomic and transcriptomic profiles for cTECs and mTECs, which identified signature molecules that characterize a developmental and functional contrast between cTECs and mTECs. Our results reveal a highly specific impact of the thymoproteasome on proteasome subunit composition in cTECs and provide an integrated trans-omics platform for further exploration of thymus biology

Limited Role of Working Time Shift in Offsetting the Increasing Occupational‐Health Cost of Heat Exposure

Climate change increases workers\u27 exposure to heat stress. To prevent heat‐related illnesses, according to occupational‐health recommendations, labor capacity must be reduced. However, this preventive measure is expected to be costly, and the costs are likely to rise as the scale and scope of climate change impacts increase over time. Shifting the start of the working day to earlier in the morning could be an effective adaptation measure for avoiding the impacts of labor capacity reduction. However, the plausibility and efficacy of such an intervention have never been quantitatively assessed. Here we investigate whether working time shifts can offset the economic impacts of labor capacity reduction due to climate change. Incorporating a temporally (1 hr) and spatially (0.5° × 0.5°) high‐resolution heat exposure index into an integrated assessment model, we calculated the working time shift necessary to offset labor capacity reduction and economic loss under hypothetical with‐ and without‐realistic‐adaptation scenarios. The results of a normative scenario analysis indicated that a global average shift of 5.7 (4.0–6.1) hours is required, assuming extreme climate conditions in the 2090s. Although a realistic (<3 hr) shift nearly halves the economic cost, a substantial cost corresponding to 1.6% (1.0–2.4%) of global total gross domestic product is expected to remain. In contrast, if stringent climate‐change mitigation is achieved, a realistic shift limits the remaining cost to 0.14% (0.12–0.47%) of global total gross domestic product. Although shifting working time is shown to be effective as an adaptation measure, climate‐change mitigation remains indispensable to minimize the impact

Giant X-ray circular dichroism in a time-reversal invariant altermagnet

X-ray circular dichroism, arising from the contrast in X-ray absorption between opposite photon helicities, serves as a spectroscopic tool to measure the magnetization of ferromagnetic materials and identify the handedness of chiral crystals. Antiferromagnets with crystallographic chirality typically lack X-ray magnetic circular dichroism because of time-reversal symmetry, yet exhibit weak X-ray natural circular dichroism. Here, we report the observation of giant natural circular dichroism in the Ni $L_3$-edge X-ray absorption of Ni$_3$TeO$_6$, a polar and chiral antiferromagnet with effective time-reversal symmetry. To unravel this intriguing phenomenon, we propose a phenomenological model that classifies the movement of photons in a chiral crystal within the same symmetry class as that of a magnetic field. The coupling of X-ray polarization with the induced magnetization yields giant X-ray natural circular dichroism, revealing the altermagnetism of Ni$_3$TeO$_6$. Our findings provide evidence for the interplay between magnetism and crystal chirality in natural optical activity. Additionally, we establish the first example of a new class of magnetic materials exhibiting circular dichroism with time-reversal symmetry.Comment: Accepted by Advanced Materials (2024.2.16) Revised title: Giant X-ray circular dichroism in a time-reversal invariant altermagnet Revised drafts: Main 14 pages, 4 figures, and SI 20 pages, 8 figure