Antimicrobial susceptibility profiles and prevalence of ESBLS among E.coli isolates recovered from people working in hospitality industry within Nairobi, Kenya

Objective: To determine the antimicrobial susceptibility profiles with key focus on ESBL-producing E.coli strains isolated from participants working in the hospitality industry in Nairobi, KenyaStudy design: A cross sectional descriptive study.Setting: Centre for Microbiology Research at Kenya Medical Research Institute, Nairobi.Subjects: A total of 323 food-handlers working within Nairobi County were recruited.Results: High resistances were recorded for Sulfamethoxazole/Trimethoprim (70%), Ampicillin (44.6%), Streptomycin (42%) and Tetracyclines (41%) while Imipenem and Cefepime were effective against 99% of the isolates. A third of all isolates were multidrug resistant. The prevalence of ESBLs was 3.4% while CMT, IRT and pAmpC-phenotypes accounted for <2%. About 1% of the ESBL-producers were also resistant to ciprofloxacin and gentamicin. The blaTEM accounted for 37%, blaSHV (25%), blaCTX-M (12%) and blaOXA-1 gene (7%). Majority of MDR strains were obtained from young individuals working in middle class hotels. Genetic relatedness of the MDR isolates was apparently influenced by the resistance profiles, hotel type and clinical characteristics.Conclusion: This study revealed that apparently healthy people working in the hospitality industry carry MDR E. coli that could potentially be transmitted to the general public. Infections by such strains could result in limited treatment options increasing the chances of patient mortality. Therefore, there is need to contain the spread of such strains through promotion of rational use of antimicrobials, properhygiene and certification of food handlers based on proper laboratory investigations

Women’s attitudes towards receiving family planning services from community health workers in rural Western Kenya.

Background: Kenya ranks among the countries in Africa with high fertility rates. In order to reverse the trends in high fertility rates, there is need to increase uptake of family planning services particularly by use of community health workers (CHWs) in providing these services in rural areas.Objective: To describe the perceptions of women towards family planning service provision by CHWs in four rural districts of Western Kenya. It is based on baseline survey data from a three-year, rural community-based family planning project funded by The David and Lucile Packard Foundation.Methods: A cross-sectional baseline survey was conducted in Western Kenya. The data presented is part of a broader the broader survey . A survey questionnaire was administered to 1,997 women (15-49 years) but only 963 cases were valid for our analyses.Results: The findings revealed that only a third of the respondents exhibited high approval for Family Planning services from CHWs. Only four out of the thirteen variables explored showed significance on attitudes towards family planning services from CHWs. These are age, level of education, knowledge about family planning benefits and districts.Conclusion: Women’s perception towards family planning services delivered by CHWs in Western region in Kenya is quite low. To improve the demand and supply for family planning services in this region, there is need to invest a substantial amount of effort into sensitization of women on the relevance of CHWs in providing family planning services.Key words: Community health workers, Women, Family Planning, Attitud

Development of a Carbonated Guyabano Juice

Soda is said to have “empty calories.” Thus, a healthier carbonated beverage was produced using guyabano, which is rich in vitamins, minerals, and flavonoids. Guyabano syrup was prepared with sugar, water, guyabano juice, and calamansi juice. To optimize the final product, guyabano syrup was mixed with carbonated water in three ratios, namely, 2:17, 3:17, and 4:17. From a preference ranking test, formulations with 3:17 and 4:17 ratio were not significantly different, but the latter was chosen for the succeeding tests based on its low rank sum. A consumer acceptability test showed that panelists “moderately liked” the appearance, taste, and sweetness of the product. On the other hand, aroma, carbonation, and the overall acceptability were “liked very much.” Physicochemical properties of guyabano juice, guyabano syrup, and carbonated guyabano juice were determined. The pure guyabano juice had 4.21 pH, 0.94% malic acid, and 15 °Bx while the guyabano syrup had a pH of 3.97, 0.69% malic acid, and 46.26 °Bx. The pH, TTA (total titratable acidity), and TSS (total soluble solids) of the finished product were 3.80, 0.21%, and 10.37 °Bx, respectively. An antioxidant capacity assay showed that the carbonated guyabano juice had 0.131 mg ascorbic acid equivalent-mL-1. There was no significant change in pH and TTA after one week storage at ambient temperature. However, yeast and mold count significantly increased and TSS significantly decreased. Thus, it is recommended that the developed carbonated guyabano juice be stored at refrigerated temperature or added with a suitable preservative

Application of MobileNets Convolutional Neural Network Model in Detecting Tomato Late Blight Disease

Late blight (LB) disease causes significant annual losses in tomato production. Early identification of this disease is crucial in halting its severity. This study aimed to leverage the strength of Convolutional Neural Networks (CNNs) in automated prediction of tomato LB. Through transfer learning, the MobileNetV3 model was trained on high-quality, well-labeled images from Kaggle datasets. The trained model was tested on different images of healthy and infected leaves taken from different real-world locations in Mbeya, Arusha, and Morogoro. Test results demonstrated the model's success in identifying LB disease, with an accuracy of 81% and a precision of 76%. The trained model has the potential to be integrated into an offline mobile app for real-time use, improving the efficiency and effectiveness of LB disease detection in tomato production. Similar methods could also be applied to detect other tomato infections. Keywords:  MobileNets; convolutional neural networks; plant diseases detection; image classification; transfer learnin

High ethionamide resistance in Mycobacterium tuberculosis strains isolated in Kenya

Background: Increasing development of tuberculosis (TB) resistance to the currently available drugs including second-line anti-TB drugs that are being used for treatment of Multi-Drug Resistant TB (MDR-TB) patients has frustrated efforts to control TB worldwide. Ethionamide (Eth) is one of the drugs used in the regimen for treatment of these patients. Objective: To determine level of Ethionamide resistance among second-line anti-tuberculosis drugs in Mycobacterium tuberculosis (MTB) strains isolated in Kenya. Design: A retrospective lab-based study involving archived strains from previous studies carried out at the Centre for Respiratory Diseases Research (CRDR), Kenya Medical Research Institute (KEMRI) from 2002 to 2007. Setting: Centre for Respiratory Diseases Research (CRDR), Kenya Medical Research Institute (KEMRI). Methods: A total of 216 MTB strains with pre-determined first-line drug susceptibility testing (DST) results were used including 78 first-line resistant to individual and combined drugs, and 138 susceptible to streptomycin, rifampicin, isoniazid and ethambutol. The strains were subjected to DST to ethionamide among other second-line. Results: Thirty two [32/216 (14.8%)] strains showed resistance to second-line drugs. Resistance to Eth was the highest [18/32 (56.3%)] including co-resistance with isoniazid [8/18 (44.4%)]. Nine [9/18 (50%)] strains were fully resistant and 9 [9/18 (50%)] were intermediate resistant to Eth. Conclusion: Unexplainable high levels of Eth resistance is a cause for concern. This will impact negatively on the outcome of management of MDR-TB especially in Kenya where the use of this drug is almost mandatory. Close monitoring of Eth before initiating individual patient management may be necessary. Keywords: Ethionamide, Resistant, MDR-TB

Improvement of Tuberculosis Laboratory Capacity on Pemba Island, Zanzibar: A Health Cooperation Project.

Low-income countries with high Tuberculosis burden have few reference laboratories able to perform TB culture. In 2006, the Zanzibar National TB Control Programme planned to decentralize TB diagnostics. The Italian Cooperation Agency with the scientific support of the "L. Spallanzani" National Institute for Infectious Diseases sustained the project through the implementation of a TB reference laboratory in a low-income country with a high prevalence of TB. The implementation steps were: 1) TB laboratory design according to the WHO standards; 2) laboratory equipment and reagent supplies for microscopy, cultures, and identification; 3) on-the-job training of the local staff; 4) web- and telemedicine-based supervision. From April 2007 to December 2010, 921 sputum samples were received from 40 peripheral laboratories: 120 TB cases were diagnosed. Of all the smear-positive cases, 74.2% were culture-positive. During the year 2010, the smear positive to culture positive rate increased up to 100%. In March 20, 2010 the Ministry of Health and Social Welfare of Zanzibar officially recognized the Public Health Laboratory- Ivo de Carneri as the National TB Reference Laboratory for the Zanzibar Archipelago. An advanced TB laboratory can represent a low cost solution to strengthen the TB diagnosis, to provide capacity building and mid-term sustainability

Detection of persistent Plasmodium spp. infections in Ugandan children after artemether-lumefantrine treatment

During a longitudinal study investigating the dynamics of malaria in Ugandan lakeshore communities, a consistently high malaria prevalence was observed in young children despite regular treatment. To explore the short-term performance of artemether-lumefantrine (AL), a pilot investigation into parasite carriage after treatment(s) was conducted in Bukoba village. A total of 163 children (aged 2–7 years) with a positive blood film and rapid antigen test were treated with AL; only 8·7% of these had elevated axillary temperatures. On day 7 and then on day 17, 40 children (26·3%) and 33 (22·3%) were positive by microscopy, respectively. Real-time PCR analysis demonstrated that multi-species Plasmodium infections were common at baseline, with 41·1% of children positive for Plasmodium falciparum/Plasmodium malariae, 9·2% for P. falciparum/ Plasmodium ovale spp. and 8·0% for all three species. Moreover, on day 17, 39·9% of children infected with falciparum malaria at baseline were again positive for the same species, and 9·2% of those infected with P. malariae at baseline were positive for P. malariae. Here, chronic multi-species malaria infections persisted in children after AL treatment(s). Better point-of-care diagnostics for non-falciparum infections are needed, as well as further investigation of AL performance in asymptomatic individuals

Enhancing food security in an era of global climate change

The goal of the workshop was to build a more strategic and integrated perspective on the threats and opportunities latent in the food / climate issue, and to discuss the hard challenges of moving forward toward common goals in a private, off-the-record setting. An executive session convened by the John F. Kennedy School of Government at Harvard University and the Venice International University on June 6-9, 2010 attracted more than 25 of the world’s leading experts from the fields of policy, science, and business to San Servolo Island for an intensive three day session (see text for a list of the participants). The discussions were off-the-record, with each participant present in his or her own capacity, rather than representing an organization. The session was one in a series on Grand Challenges of the Sustainability Transition organized by the Sustainability Science Program at Harvard University with the generous support of the Italy’s Ministry for Environment, Land and Sea. This particular session was held in cooperation with the new Mega Program on Climate Change, Agriculture and Food Security being developed by the Consultative Group on International Agricultural Research (CGIAR) and the Earth System Science Partnership. This summary report of the session is our synthesis of the main points and arguments that emerged from the discussions. It does not represent a consensus document, since no effort was made at the Session to arrive at a single consensus view. Rather, we report here on what we heard to be the major themes discussed at the session. Any errors or misrepresentations remain solely our responsibility

Enhancing Food Security in an Era of Global Climate Change: An Executive Session on Grand Challenges of the Sustainability Transition

The goal of the workshop was to build a more strategic and integrated perspective on the threats and opportunities latent in the food / climate issue, and to discuss the hard challenges of moving forward toward common goals in a private, off-the-record setting. An executive session convened by the John F. Kennedy School of Government at Harvard University and the Venice International University on June 6-9, 2010, attracted more than 25 of the world's leading experts from the fields of policy, science, and business to San Servolo Island for an intensive three- day session (see text for a list of the participants). The discussions were off-the-record, with each participant present in his or her own capacity, rather than representing an organization. The session was one in a series on Grand Challenges of the Sustainability Transition organized by the Sustainability Science Program at Harvard University with the generous support of the Italy’s Ministry for Environment, Land and Sea. This particular session was held in cooperation with the new Mega Program on Climate Change, Agriculture and Food Security being developed by the Consultative Group on International Agricultural Research (CGIAR) and the Earth System Science Partnership. This summary report of the session is our synthesis of the main points and arguments that emerged from the discussions. It does not represent a consensus document, since no effort was made at the session to arrive at a single consensus view. Rather, we report here on what we heard to be the major themes discussed at the session. Any errors or misrepresentations remain solely our responsibility