Adaptive laser link reconfiguration using constraint propagation

This paper describes Harris AI research performed on the Adaptive Link Reconfiguration (ALR) study for Rome Lab, and focuses on the application of constraint propagation to the problem of link reconfiguration for the proposed space based Strategic Defense System (SDS) Brilliant Pebbles (BP) communications system. According to the concept of operations at the time of the study, laser communications will exist between BP's and to ground entry points. Long-term links typical of RF transmission will not exist. This study addressed an initial implementation of BP's based on the Global Protection Against Limited Strikes (GPALS) SDI mission. The number of satellites and rings studied was representative of this problem. An orbital dynamics program was used to generate line-of-site data for the modeled architecture. This was input into a discrete event simulation implemented in the Harris developed COnstraint Propagation Expert System (COPES) Shell, developed initially on the Rome Lab BM/C3 study. Using a model of the network and several heuristics, the COPES shell was used to develop the Heuristic Adaptive Link Ordering (HALO) Algorithm to rank and order potential laser links according to probability of communication. A reduced set of links based on this ranking would then be used by a routing algorithm to select the next hop. This paper includes an overview of Constraint Propagation as an Artificial Intelligence technique and its embodiment in the COPES shell. It describes the design and implementation of both the simulation of the GPALS BP network and the HALO algorithm in COPES. This is described using a 59 Data Flow Diagram, State Transition Diagrams, and Structured English PDL. It describes a laser communications model and the heuristics involved in rank-ordering the potential communication links. The generation of simulation data is described along with its interface via COPES to the Harris developed View Net graphical tool for visual analysis of communications networks. Conclusions are presented, including a graphical analysis of results depicting the ordered set of links versus the set of all possible links based on the computed Bit Error Rate (BER). Finally, future research is discussed which includes enhancements to the HALO algorithm, network simulation, and the addition of an intelligent routing algorithm for BP

Bodenchemische Charakteristka entlang präferentieller Fließwege in Waldböden mit unterschiedlicher P-Verfügbarkeit

In der vorliegenden Studie wurden die präferentiellen Fließwege in verschiedenen Waldböden charakterisiert und bodenchemische Parameter im Bereich der Fließwege sowie der nicht-durchflossenen Bodenmatrix analysiert. Die untersuchten Waldstandorte unterscheiden sich signifikant hinsichtlich der Verfügbarkeit mineralischer Phosphor-Quellen (P-reich zu P-arm), was sehr wahrscheinlich die Ernährungsstrategie der Buchenbestockung an diesen Standorten wesentlich beeinflusst. Zur Charakterisierung der präferentiellen Fließwege in den Böden wurde diese mittels Farbtracer-Experimenten, digitaler Bildanalyse und statistischer Auswertung der Verteilungsmuster untersucht. Die bodenchemische Charakterisierung der Fließwege erfolgte anhand Analyse der chemischen Bindungsformen von P, Al, Fe und Mn, der C- und N-Gesamtgehalte sowie daraus berechneter Verhältnisse (z.B. C:P). Die Ergebnisse zeigen, dass sich sowohl die Fließweg-Verteilung in den Böden als auch die Verteilung der bodenchemischen Parameter an den einzelnen Standorten deutlich unterscheiden. Trotz sehr unterschiedlicher P-Gehalte im Boden wiesen die Gehalte an labilem, leicht pflanzenverfügbarem P in den organischen Auflagen aller Standorte ähnliche Größenordnungen auf. Während der P-arme Standort jedoch sehr stark sinkende labile P-Gehalte mit der Tiefe zeigte, war der Tiefengradient dieser Fraktion am P-reichen Standort deutlich schwächer ausgeprägt. Die Verhältnisse von C zu organisch gebundenem P (C:Po) waren am P-reichen Standort gering und nahmen über die intermediären bis hin zum schlecht P-versorgten Standort deutlich zu. Am P-armen Standort deuten die sehr hohen C:Po-Werte, insbesondere in der organischen Auflage, auf intensive P-Recyclingprozesse hin. Des Weiteren wurden Hinweise auf eine Anreicherung organisch gebundender P-Formen in präferentiellen Fließwegen im Vergleich zur Bodenmatrix gefunden, die mit einem erhöhten C:Po in den Fließwegen einhergehen

Modelling the impact of agroforestry on hydrology of Mara River Basin in East Africa

Land�use change is one of the main drivers of change of watershed hydrology. The effect of forestry related land�use changes (e.g. afforestation, deforestation, agroforestry) on water fluxes depends on climate, watershed characteristics and spatial scale. The Soil and Water Assessment Tool (SWAT) model was calibrated, validated and used to simulate the impact of agroforestry on the water balance in Mara River Basin (MRB) in East Africa. Model performance was assessed by Nash-Sutcliffe Efficiency (NSE) and Kling-Gupta Efficiency (KGE). The NSE (and KGE) values for calibration and validation were: 0.77 (0.88) and 0.74 (0.85) for the Nyangores sub-watershed, and 0.78 (0.89) and 0.79 (0.63) for the entire MRB. It was found that agroforestry in the watershed would generally reduce surface runoff, mainly due to enhanced infiltration. However, it would also increase evapotranspiration and consequently reduce the baseflow and the overall water yield, which was attributed to increased water use by trees. Spatial scale was found to have a significant effect on water balance; the impact of agroforestry was higher at the smaller headwater catchment (Nyangores) than for the larger watershed (entire MRB). However, the rate of change in water yield with increase in area under agroforestry was different for the two and could be attributed to the spatial variability of climate within MRB. Our results suggest that direct extrapolation of the findings from a small sub-catchment to a larger watershed may not always be accurate. These findings could guide watershed managers on the level of trade-offs to make between reduced water yields and other benefits (e.g. soil erosion control, improved soil productivity) offered by agroforestry. This article is protected by copyright. All rights reserved

The strategic use of terminology in restorative justice for persons harmed by sexual violence

An argument for the importance of strategically selected terminology in the practice of restorative justice in sexual violence cases is presented through reviews of restorative justice, communication, social constructivist and feminist literature. The significance of language and its impact on those who use it and hear it is established from its use in classical antiquity, psychotherapy and semantics. The use of the terms ‘victim’ and ‘survivor’ is explored in the fields of legal definitions and feminist theory. Reports in the existing restorative justice literature are used to bring together the literature on the impact of the use of terminology and the legal and feminist understandings of the significance of the use of the terms ‘victim’ and ‘survivor’. We argue that the restorative justice practitioner has a crucial role in guiding the person harmed in sexual violence cases in the strategic use of ‘victim’ and ‘survivor’ to enhance the positive impact of terminology on the persons harmed in acts of sexual violence. Conclusions from our explorations support the creation of a proposed sexual violence restorative justice situational map for use as a navigational aid in restorative justice practice in sexual violence cases.fals

α5β1 Integrin-Mediated Adhesion to Fibronectin Is Required for Axis Elongation and Somitogenesis in Mice

The arginine-glycine-aspartate (RGD) motif in fibronectin (FN) represents the major binding site for α5β1 and αvβ3 integrins. Mice lacking a functional RGD motif in FN (FNRGE/RGE) or α5 integrin develop identical phenotypes characterized by embryonic lethality and a severely shortened posterior trunk with kinked neural tubes. Here we show that the FNRGE/RGE embryos arrest both segmentation and axis elongation. The arrest is evident at about E9.0, corresponding to a stage when gastrulation ceases and the tail bud-derived presomitic mesoderm (PSM) induces α5 integrin expression and assumes axis elongation. At this stage cells of the posterior part of the PSM in wild type embryos are tightly coordinated, express somitic oscillator and cyclic genes required for segmentation, and form a tapered tail bud that extends caudally. In contrast, the posterior PSM cells in FNRGE/RGE embryos lost their tight associations, formed a blunt tail bud unable to extend the body axis, failed to induce the synchronised expression of Notch1 and cyclic genes and cease the formation of new somites. Mechanistically, the interaction of PSM cells with the RGD motif of FN is required for dynamic formation of lamellipodia allowing motility and cell-cell contact formation, as these processes fail when wild type PSM cells are seeded into a FN matrix derived from FNRGE/RGE fibroblasts. Thus, α5β1-mediated adhesion to FN in the PSM regulates the dynamics of membrane protrusions and cell-to-cell communication essential for elongation and segmentation of the body axis

Analysis of her1 and her7 Mutants Reveals a Spatio Temporal Separation of the Somite Clock Module

Somitogenesis is controlled by a genetic network consisting of an oscillator (clock) and a gradient (wavefront). The “hairy and Enhancer of Split”- related (her) genes act downstream of the Delta/Notch (D/N) signaling pathway, and are crucial components of the segmentation clock. Due to genome duplication events, the zebrafish genome, possesses two gene copies of the mouse Hes7 homologue: her1 and her7. To better understand the functional consequences of this gene duplication, and to determine possible independent roles for these two genes during segmentation, two zebrafish mutants her1hu2124 and her7hu2526 were analyzed. In the course of embryonic development, her1hu2124 mutants exhibit disruption of the three anterior-most somite borders, whereas her7hu2526 mutants display somite border defects restricted to somites 8 (+/−3) to 17 (+/−3) along the anterior-posterior axis. Analysis of the molecular defects in her1hu2124 mutants reveals a her1 auto regulatory feedback loop during early somitogenesis that is crucial for correct patterning and independent of her7 oscillation. This feedback loop appears to be restricted to early segmentation, as cyclic her1 expression is restored in her1hu2124 embryos at later stages of development. Moreover, only the anterior deltaC expression pattern is disrupted in the presomitic mesoderm of her1hu2124 mutants, while the posterior expression pattern of deltaC remains unaltered. Together, this data indicates the existence of an independent and genetically separable anterior and posterior deltaC clock modules in the presomitic mesdorm (PSM)

Time-Lapse Analysis and Mathematical Characterization Elucidate Novel Mechanisms Underlying Muscle Morphogenesis

Skeletal muscle morphogenesis transforms short muscle precursor cells into long, multinucleate myotubes that anchor to tendons via the myotendinous junction (MTJ). In vertebrates, a great deal is known about muscle specification as well as how somitic cells, as a cohort, generate the early myotome. However, the cellular mechanisms that generate long muscle fibers from short cells and the molecular factors that limit elongation are unknown. We show that zebrafish fast muscle fiber morphogenesis consists of three discrete phases: short precursor cells, intercalation/elongation, and boundary capture/myotube formation. In the first phase, cells exhibit randomly directed protrusive activity. The second phase, intercalation/elongation, proceeds via a two-step process: protrusion extension and filling. This repetition of protrusion extension and filling continues until both the anterior and posterior ends of the muscle fiber reach the MTJ. Finally, both ends of the muscle fiber anchor to the MTJ (boundary capture) and undergo further morphogenetic changes as they adopt the stereotypical, cylindrical shape of myotubes. We find that the basement membrane protein laminin is required for efficient elongation, proper fiber orientation, and boundary capture. These early muscle defects in the absence of either lamininβ1 or lamininγ1 contrast with later dystrophic phenotypes in lamininα2 mutant embryos, indicating discrete roles for different laminin chains during early muscle development. Surprisingly, genetic mosaic analysis suggests that boundary capture is a cell-autonomous phenomenon. Taken together, our results define three phases of muscle fiber morphogenesis and show that the critical second phase of elongation proceeds by a repetitive process of protrusion extension and protrusion filling. Furthermore, we show that laminin is a novel and critical molecular cue mediating fiber orientation and limiting muscle cell length

Molecular techniques for pathogen identification and fungus detection in the environment

Many species of fungi can cause disease in plants, animals and humans. Accurate and robust detection and quantification of fungi is essential for diagnosis, modeling and surveillance. Also direct detection of fungi enables a deeper understanding of natural microbial communities, particularly as a great many fungi are difficult or impossible to cultivate. In the last decade, effective amplification platforms, probe development and various quantitative PCR technologies have revolutionized research on fungal detection and identification. Examples of the latest technology in fungal detection and differentiation are discussed here

PEG−peptide conjugates

The remarkable diversity of the self-assembly behavior of PEG−peptides is reviewed, including self-assemblies formed by PEG−peptides with β-sheet and α-helical (coiled-coil) peptide sequences. The modes of self-assembly in solution and in the solid state are discussed. Additionally, applications in bionanotechnology and synthetic materials science are summarized