Additional file 1: of The association between high-sensitivity C-reactive protein and metabolic risk factors in black and white South African women: a cross-sectional study

Table S1. Adjusted associations between insulin resistance (HOMA-IR) and hsCRP in black and white South African women. Data represents β-coefficients [95% confidence interval] and adjusted-R2. Model 1: hsCRP + age + race/ethnicity + (hsCRP x race/ethnicity interaction); Model 2: (Model 1) + SES + lifestyle factors; Model 3: (Model 2) + WC. hsCRP, C-reactive protein; hsCRP x race/ethnicity, interaction between hsCRP and race/ethnicity; WC, waist circumference; SES, socio-economic status; ln(HOMA-IR), natural log of homeostatic model assessment. *p < 0.05 and **p < 0.001 (PDF 1343 kb

Additional file 2: of The association between high-sensitivity C-reactive protein and metabolic risk factors in black and white South African women: a cross-sectional study

Table S2. Adjusted associations between triglycerides and hsCRP in black and white South African women. Data represents β-coefficients [95% confidence interval] and adjusted-R2. Model 1: hsCRP + age + race/ethnicity + (hsCRP x race/ethnicity interaction); Model 2: (Model 1) + SES + lifestyle factors; Model 3: (Model 2) + WC. hsCRP, C-reactive protein; hsCRP x race/ethnicity, interaction between hsCRP and race/ethnicity; WC, waist circumference; SES, socio-economic status; ln(TG), natural log of triglycerides. *p < 0.05 and **p < 0.001 (PDF 545 kb

Additional file 4: of The association between high-sensitivity C-reactive protein and metabolic risk factors in black and white South African women: a cross-sectional study

Table S4. Adjusted associations between HDL-C and hsCRP in black and white South African women. Data represents β-coefficients [95% confidence interval] and adjusted-R2. Model 1: hsCRP + age + race/ethnicity + (hsCRP x race/ethnicity interaction); Model 2: (Model 1) + SES + lifestyle factors; Model 3: (Model 2) + WC. hsCRP, C-reactive protein; hsCRP x race/ethnicity, interaction between hsCRP and race/ethnicity; WC, waist circumference; SES, socio-economic status; ln(HDL-C), natural log of high-density lipoprotein cholesterol. *p < 0.05 and **p < 0.001 (PDF 549 kb

Additional file 3: of The association between high-sensitivity C-reactive protein and metabolic risk factors in black and white South African women: a cross-sectional study

Table S3. Adjusted associations between total cholesterol and hsCRP in black and white South African women. Data represents β-coefficients [95% confidence interval] and adjusted-R2. Model 1: hsCRP + age + race/ethnicity + (hsCRP x race/ethnicity interaction); Model 2: (Model 1) + SES + lifestyle factors; Model 3: (Model 2) + WC. hsCRP, C-reactive protein; hsCRP x race/ethnicity, interaction between hsCRP and race/ethnicity; WC, waist circumference; SES, socio-economic status; ln(TC), natural log of total cholesterol. *p < 0.05 and **p < 0.001 (PDF 542 kb

Additional file 5: of The association between high-sensitivity C-reactive protein and metabolic risk factors in black and white South African women: a cross-sectional study

Table S5. Adjusted associations between LDL-C and hsCRP in black and white South African women. Data represents β-coefficients [95% confidence interval] and adjusted-R2. Model 1: hsCRP + age + race/ethnicity + (hsCRP x race/ethnicity interaction); Model 2: (Model 1) + SES + lifestyle factors; Model 3: (Model 2) + WC. hsCRP, C-reactive protein; hsCRP x race/ethnicity, interaction between hsCRP and race/ethnicity; WC, waist circumference; SES, socio-economic status; ln(LDL-C), natural log of low-density lipoprotein cholesterol. *p < 0.05 and **p < 0.001. (PDF 565 kb

Additional file 1: Figure S1. of Association between ethnicity and obesity with high-density lipoprotein (HDL) function and subclass distribution

Paraoxonase protein expression in white and black women. Isolated HDL (a–c) and serum (d–f) from each participant was randomly loaded and run on reducing 12.5 % SDS-PAGE gels and transferred to nitrocellulose membrane. Ponceau S staining was used to confirm equal loading. Blots were probed with mouse anti-PON-1 antibody. WN = White normal-weight. WO = White obese. BN = Black normal weight. BO = Black obese. Figure S2. PAF-AH protein expression in white and black women. Isolated HDL (a–c) and serum (d–f) from each participant was randomly loaded and run on reducing 12.5 % SDS-PAGE gels and transferred to nitrocellulose membrane. Ponceau S staining was used to confirm equal loading. Blots were probed with rabbit anti-PAF-AH antibody. WN = White normal-weight. WO = White obese. BN = Black normal weight. BO = Black obese. Figure S3. Vascular Cell Adhesion Molecule (VCAM) expression in endothelial cells treated with HDL. HUVEC cells were treated overnight with 10 μg/ml subject HDL. Cells were exposed to 20 ng/ml tumour necrosis factor (TNF) for 8 h. Cell lysates were harvested and stored in RNAprotect reagent prior to RNA extraction, followed by cDNA synthesis and quantitative real time PCR. Results are presented relative to a no-HDL treatment control. Results are means of 3 independent experiments ± SEM. Figure S4. Antioxidant capacity of isolated HDL. Isolated subject HDL was diluted in phosphate buffer and measured using the Oxygen Radical Absorbance Capacity (ORAC) assay. (PDF 403 kb