Paraoxonase protein expression in white and black women. Isolated HDL (a–c) and serum (d–f) from each participant was randomly loaded and run on reducing 12.5 % SDS-PAGE gels and transferred to nitrocellulose membrane. Ponceau S staining was used to confirm equal loading. Blots were probed with mouse anti-PON-1 antibody. WN = White normal-weight. WO = White obese. BN = Black normal weight. BO = Black obese. Figure S2. PAF-AH protein expression in white and black women. Isolated HDL (a–c) and serum (d–f) from each participant was randomly loaded and run on reducing 12.5 % SDS-PAGE gels and transferred to nitrocellulose membrane. Ponceau S staining was used to confirm equal loading. Blots were probed with rabbit anti-PAF-AH antibody. WN = White normal-weight. WO = White obese. BN = Black normal weight. BO = Black obese. Figure S3. Vascular Cell Adhesion Molecule (VCAM) expression in endothelial cells treated with HDL. HUVEC cells were treated overnight with 10 μg/ml subject HDL. Cells were exposed to 20 ng/ml tumour necrosis factor (TNF) for 8 h. Cell lysates were harvested and stored in RNAprotect reagent prior to RNA extraction, followed by cDNA synthesis and quantitative real time PCR. Results are presented relative to a no-HDL treatment control. Results are means of 3 independent experiments ± SEM. Figure S4. Antioxidant capacity of isolated HDL. Isolated subject HDL was diluted in phosphate buffer and measured using the Oxygen Radical Absorbance Capacity (ORAC) assay. (PDF 403 kb
