Solidarische Landwirtschaft (SoLaWi): Forschungsumfeld – Stand der Forschung und Forschungsbedarfe

The question of interest is the current state for research and the need of new research in the field of Community Supported Agriculture (CSA) in Germany. The methods include a literature study and six qualitative interviews of experts. As a result, the current state of research in the beginning of the year 2014 mainly comprises student research and six extensive studies. Consequently, there is still a high need for research in the field of CSA

Equine induced Pluripotent Stem Cell culture

Ground-breaking work by Takahashi and Yamanaka in 2006 demonstrated that non-embryonic cells can be reprogrammed into pluripotent stem cells (PSCs) by forcing the expression of a defined set of transcription factors in culture, thus overcoming ethical concerns linked to embryonic stem cells. Induced PSCs have since revolutionized biomedical research, holding tremendous potential also in other areas such as livestock production and species conservation. iPSCs exhibit broad accessibility, having been derived from a multitude of cell types and species. Apart from humans, iPSCs hold particular medical promise in the horse. The potential of iPSCs has been shown in a variety of biomedical contexts in the horse. However, progress in generating therapeutically useful equine iPSCs has lagged behind that reported in humans, with the generation of footprint-free iPSCs using non-integrative reprogramming approaches having proved particularly challenging. A greater understanding of the underlying molecular pathways and essential factors required for the generation, and maintenance of equine iPSCs and their differentiation into relevant lineages will be critical for realising their significant potential in veterinary regenerative medicine. This article outlines up-to-date protocols for the successful culture of equine iPSC, including colony selection, expansion, and adaptation to feeder-free conditions

Health and Safety Events for Latino Families: Collaborating to Create \u3ci\u3eEl Día de los Niños Celebración\u3c/i\u3e

Latino immigrants to rural counties within North Carolina are at an increased risk for experiencing injury, health complications, and chronic illness. This is due largely to the fact that many new immigrants arrive with limited knowledge of the health and safety risks that are present in their communities. To reduce the incidence of injury and health complications, programs must be developed to increase local awareness of these risks. This article outlines the collaborative efforts of one rural North Carolina community to develop and implement a community-based health and safety event for Latino families

Arthrofibrosis after TKA - Influence factors on the absolute flexion and gain in flexion after manipulation under anaesthesia

<p>Abstract</p> <p>Background</p> <p>Stiffness with decreased range of motion (ROM) has been described as a frustrating complication after TKA. If all methods of physiotherapeutic treatment have been exhausted trying to develop ROM, manipulation under anaesthesia (MUA) can be discussed. The aim of the present study was to show the effect of MUA and to determine the influence of BMI, number of previous surgical procedures, pre-MUA ROM and timing of MUA for the results after MUA in regard to absolute flexion and gain in flexion.</p> <p>Methods</p> <p>858 patients underwent TKA at our institution between 2004 and 2009. 39 of these patients underwent MUA because of postoperative knee stiffness. The data were retrospective analysed for the influence of BMI, pre-MUA flexion (</≥ 70°), timing of MUA (>/≤ 30 days after TKA) and number of previous surgery on the results after MUA (absolute Flexion/gain in flexion).</p> <p>Results</p> <p>The prevalence for stiffness after TKA was 4.54%. There was a statistically significant improvement in flexion not only directly after MUA but also 6 weeks after MUA. Patients with two or more previous operations before TKA showed statistically significant worse results six weeks after MUA in absolute flexion and gain in flexion</p> <p>(p = 0.039) than patients with one or two previous operations. No statistical significance in absolute flexion (p = 0.655) and gain in flexion (p = 0.328) after MUA between "early" and "late" was detected. The stiffer knees with a flexion below 70° showed significantly worse results (p = 0.044) in absolute flexion six weeks after MUA, but they also had statistical statistically better results with regard to gain in flexion (p ≤ 0.001).</p> <p>Conclusion</p> <p>MUA is a good instrument for improving ROM after TKA. The time between TKA and MUA seems less important, so different types of physiotherapeutic treatment could be tried before the procedure is started. MUA in patients with many previous operations and a flexion of less than 70° before MUA is not as effective as in other patients, but they also benefit from MUA.</p

PET/CT of the Spleen with Gallium-Oxine-Labeled, Heat-Damaged Red Blood Cells: Clinical Experience and Technical Aspects

Several scintigraphic techniques have been supplemented or replaced by PET/CT methods because of their superior sensitivity, high resolution, and absolute activity quantification capability. The purpose of this project was the development of a PET tracer for splenic imaging, its radiopharmaceutical validation, and its application in selected patients in whom unclear constellations of findings could not be resolved with established imaging methods. Heat-damaged red blood cells (RBCs) were labeled with [ 68 Ga]gallium-oxine, which was produced from [ 68 Ga]gallium and 8-Hydroxyquinoline (oxine) on an automated synthesizer. Ten patients underwent [ 68 Ga]gallium-oxine-RBC-PET/CT for the classification of eleven unclear lesions (3 intra-, 8 extrapancreatic). [ 68 Ga]gallium-oxine and [68Ga]gallium-oxine-labeled RBCs could be synthesized reproducibly and reliably. The products met GMP quality standards. The tracer showed high accumulation in splenic tissue. Of the 11 lesions evaluated by PET/CT, 3 were correctly classified as non-splenic, 6 as splenic, 1 as equivocal, and 1 lesion as a splenic hypoplasia. All lesions classified as non-splenic were malignant, and all lesions classified as splenic did not show malignant features during follow-up. PET/CT imaging of the spleen with [ 68 Ga]gallium-oxine-labeled, heat-damaged RBCs is feasible and allowed differentiation of splenic from non-splenic tissues, and the diagnosis of splenic anomalies

Fine for others but not for me: The role of perspective in patients’ perception of artificial intelligence in online medical platforms

In the near future, online medical platforms enabled by artificial intelligence (AI) technology will become increasingly more prevalent, allowing patients to use them directly without having to consult a human doctor. However, there is still little research from the patient's perspective on such AI-enabled tools. We, therefore, conducted a preregistered 2x3 between-subjects experiment (N = 266) to examine the influence of perspective (oneself vs. average person) and source of advice (AI vs. male physician vs. female physician) on the perception of a medical diagnosis and corresponding treatment recommendations. Results of robust ANOVAs showed a statistically significant interaction between the source of advice and perspective for all three dependent variables (i.e., evaluation of the diagnosis, evaluation of the treatment recommendation, and risk perception). People prefer the advice of human doctors to an AI when it comes to their own situation. In contrast, the participants made no differences between the sources of medical advice when it comes to assessing the situation of an average person. Our study contributes to a better understanding of the patient's perspective of modern digital health technology. As our findings suggest the perception of AI-enabled diagnostic tools is more critical when it comes to oneself, future research should examine the relevant factors that influence this perception

Toxic cyanobacteria in Svalbard: chemical diversity of microcystins detected using a liquid chromatography mass spectrometry precursor ion screening method

Cyanobacteria synthesize a large variety of secondary metabolites including toxins. Microcystins (MCs) with hepato- and neurotoxic potential are well studied in bloom-forming planktonic species of temperate and tropical regions. Cyanobacterial biofilms thriving in the polar regions have recently emerged as a rich source for cyanobacterial secondary metabolites including previously undescribed congeners of microcystin. However, detection and detailed identification of these compounds is difficult due to unusual sample matrices and structural congeners produced. We here report a time-efficient liquid chromatography-mass spectrometry (LC-MS) precursor ion screening method that facilitates microcystin detection and identification. We applied this method to detect six different MC congeners in 8 out of 26 microbial mat samples of the Svalbard Archipelago in the Arctic. The congeners, of which [Asp3, ADMAdda5, Dhb7] MC-LR was most abundant, were similar to those reported in other polar habitats. Microcystins were also determined using an Adda-specific enzyme-linked immunosorbent assay (Adda-ELISA). Nostoc sp. was identified as a putative toxin producer using molecular methods that targeted 16S rRNA genes and genes involved in microcystin production. The mcy genes detected showed highest similarities to other Arctic or Antarctic sequences. The LC-MS precursor ion screening method could be useful for microcystin detection in unusual matrices such as benthic biofilms or liche