Reconceptualising post-PhD research pathways: a model to create new postdoctoral positions and improve the quality of postdoctoral training in Australia

Focusing on the developmental needs of early career postdoctoral fellows – the lifeblood of an internationally competitive researchintensive university – this paper suggests an inextricably linked, two pronged approach to improving research performance at Australian universities. The first is to reconceptualise post-PhD research pathways and in doing so conceive a mechanism for creating new postdoctoral positions; the second is to develop a coherent programme of policies, processes and practices in postdoctoral education and training. In this way, Australian universities will increase their success in attracting and retaining the brightest and best postdoctoral students from all over the world and thereby improve research performanc

The D coefficient in neutron beta decay in effective field theory

In this paper we explore the time-reversal-odd triple-correlation coefficient in neutron beta decay, the so-called "D coefficient", using heavy-baryon effective field theory with photon degrees of freedom. We find that this framework allows us to reproduce the known results for the contribution which comes from final-state interactions, and also to discuss higher-order corrections. In particular we are able to show that in the heavy-baryon limit all electromagnetic contributions vanish. By calculating the leading correction to the known result, we give a final expression which is accurate to better than 1%. Hence we extend downwards the range over which the D coefficient could be used to explore time-violation from new physics.Comment: 12c pages, 3 eps figures Version accepted for publication in Physics Letters B; minor changes of wordin

El terreno Nico-Pérez (Uruguay) y su herencia arcaica y paleoproterozoica

A U-Pb SHRIMP zircon geochronological study was carried out in the Nico Pérez Terrane in the central-eastern portion of Uruguay with the aim of constraining its geological evolution and its cratonic affinity. Nico Pérez Terrane is made up by a mosaic of tectonic blocks with diferente lithologies and sizes. This terrane is limited to the west by the Piedra Alta Terrane through the Sarandí del Yí shear zone, and to the east, is in tectonic contact through the Retamosa thrust with the Dom Feliciano Belt (Brasiliano orogenic cycle). Lithologically, the Nico Pérez Terrane is composed by medium to high grade metamorphic rock contained in three tectonic blocks (Pavas, Valentines and Rivera blocks) represented mainly by granitoids, as well as ortho and parametamorphic rocks, such as amphibolites, metapyroxenites, BIFs, schists, quartzites with fuchsite, among others. Archean inheritance, Rhyacian, Statherian, and Neoproterozoic magmatic ages are reported here. Similar ages, which show Archaean inheritance and Neoproterozoic imprint, reported in units of the Piedra Alta Terrane modify the idea that the Río de La Plata Craton only corresponds to a juvenile Paleoproterozoic tectonic unit. This, together with recently published geophysical information, supports the cratonic affinity of the Nico Pérez Terrane with the Río de La Plata Craton.Un estudio geocronológico U-Pb SHRIMP en circón fue realizado en rocas del Terreno Nico Pérez, con el objetivo de circunscribir la evolución geológica y su afinidad cratónica. Este terreno, localizado en la porción central del Uruguay, está constituído por un mosaico de tres bloques tectónicos y se encuentra limitado al oeste por el Terreno Piedra Alta a través de la zona de cizallamiento Sarandí del Yí. Al este, se encuentra en contacto tectónico a través del corrimiento Retamosa con el Cinturón Dom Feliciano (Ciclo Orogénico Brasiliano). Litológicamente, el Terreno Nico Pérez está compuesto por rocas metamórficas de grado medio a alto (bloques Pavas, Valentines y Rivera) representados principalmente por granitoides, así como por rocas orto- y parametamórficas, tales como anfibolitas, metapiroxenitas, BIFs, esquistos, cuarcitas con fucsita, entre otras. Se presenta en este trabajo un conjunto de edades magmáticas que poseen herencia arqueana, y edades riacianas, estaterianas y neoproterozoicas. Edades similares a estas, que muestran herencia arqueana e impronta neoproterozoica, reportadas en unidades del Terreno Piedra Alta, modifican la idea de que el Cratón del Río de La Plata corresponde a una unidad tectónica paleoproterozoica juvenil. Esto, junto con la información geofísica publicada recientemente, respalda la afinidad cratónica del Terreno Nico Pérez con el Cratón del Río de La Plata

Exoplanets around Low-mass Stars Unveiled by K2

We present the detection and follow-up observations of planetary candidates around low-mass stars observed by the K2 mission. Based on light-curve analysis, adaptive-optics imaging, and optical spectroscopy at low and high resolution (including radial velocity measurements), we validate 16 planets around 12 low-mass stars observed during K2 campaigns 5-10. Among the 16 planets, 12 are newly validated, with orbital periods ranging from 0.96-33 days. For one of the planets (K2-151b) we present ground-based transit photometry, allowing us to refine the ephemerides. Combining our K2 M-dwarf planets together with the validated or confirmed planets found previously, we investigate the dependence of planet radius $R_p$ on stellar insolation and metallicity [Fe/H]. We confirm that for periods $P\lesssim 2$ days, planets with a radius $R_p\gtrsim 2\,R_\oplus$ are less common than planets with a radius between 1-2$\,R_\oplus$. We also see a hint of the "radius valley" between 1.5 and 2$\,R_\oplus$ that has been seen for close-in planets around FGK stars. These features in the radius/period distribution could be attributed to photoevaporation of planetary envelopes by high-energy photons from the host star, as they have for FGK stars. For the M dwarfs, though, the features are not as well defined, and we cannot rule out other explanations such as atmospheric loss from internal planetary heat sources, or truncation of the protoplanetary disk. There also appears to be a relation between planet size and metallicity: those few planets larger than about 3 $R_\oplus$ are found around the most metal-rich M dwarfs.Comment: 29 pages, 21 figures, 6 tables, Accepted in Astronomical Journa

Identification of precursor transcripts for 6 novel miRNAs expands the diversity on the genomic organisation and expression of miRNA genes in rice

<p>Abstract</p> <p>Background</p> <p>The plant miRNAs represent an important class of endogenous small RNAs that guide cleavage of an mRNA target or repress its translation to control development and adaptation to stresses. MiRNAs are nuclear-encoded genes transcribed by RNA polymerase II, producing a primary precursor that is subsequently processed by DCL1 an RNase III Dicer-like protein.</p> <p>In rice hundreds of miRNAs have been described or predicted, but little is known on their genes and precursors which are important criteria to distinguish them from siRNAs. Here we develop a combination of experimental approaches to detect novel miRNAs in rice, identify their precursor transcripts and genes and predict or validate their mRNA targets.</p> <p>Results</p> <p>We produced four cDNA libraries from small RNA fractions extracted from distinct rice tissues. By <it>in silico </it>analysis we selected 6 potential novel miRNAs, and confirmed that their expression requires OsDCL1. We predicted their targets and used 5'RACE to validate cleavage for three of them, targeting a PPR, an SPX domain protein and a GT-like transcription factor respectively.</p> <p>In addition, we identified precursor transcripts for the 6 miRNAs expressed in rice, showing that these precursors can be efficiently processed using a transient expression assay in transfected <it>Nicotiana benthamiana </it>leaves. Most interestingly, we describe two precursors producing tandem miRNAs, but in distinct arrays. We focus on one of them encoding osa-miR159a.2, a novel miRNA produced from the same stem-loop structure encoding the conserved osa-miR159a.1. We show that this dual osa-miR159a.2-osa-miR159a.1 structure is conserved in distant rice species and maize. Finally we show that the predicted mRNA target of osa-miR159a.2 encoding a GT-like transcription factor is cleaved <it>in vivo </it>at the expected site.</p> <p>Conclusion</p> <p>The combination of approaches developed here identified six novel miRNAs expressed in rice which can be clearly distinguished from siRNAs. Importantly, we show that two miRNAs can be produced from a single precursor, either from tandem stem-loops or tandemly arrayed in a single stem-loop. This suggests that processing of these precursors could be an important regulatory step to produce one or more functional miRNAs in plants and perhaps coordinate cleavage of distinct targets in the same plant tissue.</p

Wnt5a Increases Cardiac Gene Expressions of Cultured Human Circulating Progenitor Cells via a PKC Delta Activation

Background: Wnt signaling controls the balance between stem cell proliferation and differentiation and body patterning throughout development. Previous data demonstrated that non-canonical Wnts (Wnt5a, Wnt11) increased cardiac gene expression of circulating endothelial progenitor cells (EPC) and bone marrow-derived stem cells cultured in vitro. Since previous studies suggested a contribution of the protein kinase C (PKC) family to the Wnt5a-induced signalling, we investigated which PKC isoforms are activated by non-canonical Wnt5a in human EPC. Methodology/Principal Findings: Immunoblot experiments demonstrated that Wnt5a selectively activated the novel PKC isoform, PKC delta, as evidenced by phosphorylation and translocation. In contrast, the classical Ca2+-dependent PKC isoforms, PKC alpha and beta2, and one of the other novel PKC isoforms, PKC epsilon, were not activated by Wnt5a. The PKC delta inhibitor rottlerin significantly blocked co-culture-induced cardiac differentiation in vitro, whereas inhibitors directed against the classical Ca2+-dependent PKC isoforms or a PKC epsilon-inhibitory peptide did not block cardiac differentiation. In accordance, EPC derived from PKC delta heterozygous mice exhibited a significant reduction of Wnt5a-induced cardiac gene expression compared to wild type mice derived EPC. Conclusions/Significance: These data indicate that Wnt5a enhances cardiac gene expressions of EPC via an activation of PKC delta

AvBD1 nucleotide polymorphisms, peptide antimicrobial activities and microbial colonisation of the broiler chicken gut

Abstract Background The importance of poultry as a global source of protein underpins the chicken genome and associated SNP data as key tools in selecting and breeding healthy robust birds with improved disease resistance. SNPs affecting host peptides involved in the innate defences tend to be rare, but three non-synonymous SNPs in the avian β-defensin (AvBD1) gene encoding the variant peptides NYH, SSY and NYY were identified that segregated specifically to three lines of commercial broiler chickens Line X (LX), Line Y(LY) and Line Z. The impacts of such amino acid changes on peptide antimicrobial properties were analysed in vitro and described in relation to the caecal microbiota and gut health of LX and LY birds. Results Time-kill and radial immune diffusion assays indicated all three peptides to have antimicrobial properties against gram negative and positive bacteria with a hierarchy of NYH > SSY > NYY. Calcein leakage assays supported AvBD1 NYH as the most potent membrane permeabilising agent although no significant differences in secondary structure were identified to explain this. However, distinct claw regions, identified by 3D modelling and proposed to play a key role in microbial membrane attachment, and permeation, were more distinct in the NYH model. In vivo AvBD1 synthesis was detected in the bird gut epithelia. Analyses of the caecal gut microbiota of young day 4 birds suggested trends in Lactobacilli sp. colonisation at days 4 (9% LX vs × 30% LY) and 28 (20% LX vs 12% LY) respectively, but these were not statistically significant (P > 0.05). Conclusion Amino acid changes altering the killing capacity of the AvBD1 peptide were associated with two different bird lines, but such changes did not impact significantly on caecal gut microbiota