Replacement of fish meal by a mixture of vegetal proteins and vegetal proteins combined with prebiotics in Gilt-head sea bream diets (Sparus aurata)

Three diets have been formulated to evaluate the effect of prebiotec (dry hydrolyzed intestinal mucosa) by supplementation in plant protein diets of gilt-head sea bream of 273g (average initial weight) for 60 days. The diets manufactured to be isonitrogenous (47% crude Protein, CP) and isolipidic (17% crude Lipid, CL) , two diets of them with total replacement of fish meal by a mixture of vegetable proteins, with and without prebiotec 0% Fish meal FM0 & 0% fish meal plus prebiotec FM0 + P. The third diet as the unique protein source 100% fish meal FM100. The growth, nutritional, biometric and histological parameters have been determined at the end of trial, fish reached weights 284, 303 and 407g in the FM0, FM0+P, and FM100 respectively. A statistical differences were observed between FM100 and other two (FM0 & FM0+P) diets in specific growth rate (SGR), Daily feeding rate (DFR), feed conversion ratio (FCR) and protein efficiency ratio (PER). Biometric indices show significant differences between FM100 and other two (FM0 & FM0+P) in condition factor (CF), viscerosomatic index (VSI), hepatosomatic index (HSI) and meat index (MI) as they were significantly decreasing by increasing FM replacement percent, also there were significant differences between FM100 and FM0 in Headless index (HI). The index of the non-edible parts (NEPI) was significantly higher in (FM0 & FM100) than FM100 while there were no significant differences between all diets in mesenteric fat index (MFI). Moreover, body composition was affected by the substitution with vegetable proteins, there was a decrease in water, protein and ash content and an increase of lipid and energy content from the start to the end of the trial. At the end of trial fat content was higher in the gilt-head sea bream fed FM100 diet followed by FM0+P and the least amount of fat was in gilt-head sea bream fed with feed FM0 when there were no significant differences in energy content in all groups. Protein and ash content also presents significant differences between the gilt-head sea bream that fed with feed FM0 and the gilt-head sea bream that fed other diets (FM0+P, FM100) where they were higher in FM0. The histological parameters of gut and liver not affected by different experimental diets. The morphological evaluation showed all fish in intermediate case except in FM0+P there was a severe fat accumulation within the hepatocytes. . The supplementation of prebiotec (dry hydrolyzed intestinal mucosa) was not useful contrary to the results of Tortosa, (2004) may be result to the parasite infections during the trial. The results of the current study showed that the immune role of the prebiotec was not enough to face the parasite infection and it is not recommended to use as a supplementation in vegetable meals in case of presence of parasites risk

Evaluation of soybean meal as protein source for Argyrosomus Regius (Asso, 1801) (Sciaenidae)

[EN] The meagre (Argyrosomus regius) is a carnivorous fish which requires diets with higher protein content, causing an increment in diets cost. A way to diminish this cost is to use vegetable meals like soybean meal (SB). So the aim of this trial was to determine the optimum inclusion level of defatted soybean meal in experimental diets for this species. 800 fishes (165 g) were distributed in 8 tanks, two replicates per treatment. Four isoproteic (50% CP) and isolipidic (17% Cl) diets were formulated with four levels of soybean meal inclusion, 0, 15, 30 and 45%. The trial lasted 107 days. Meagre fed diets 15 and 30% obtained the highest final weight. There were no significant differences among treatments in the feed conversion rate (FCR) and the protein efficiency (PER). According to the quadratic regression, the optimum SB inclusion to maximize thermal growth coefficient (TGC) was 26.4% and for FCR was 27.6%. No significant differences were observed in energy, protein and amino acid retention among diets. The inclusion of SB in meagre diets can generate a decrease in the use of fish meal and in turn reduce the cost of producing meagre Mediterranean aquaculture industry.Velazco Vargas, JL.; Martínez-Llorens, S.; Jover Cerda, M.; Tomas-Vidal, A. (2013). Evaluation of soybean meal as protein source for Argyrosomus Regius (Asso, 1801) (Sciaenidae). International Journal of Fisheries and Aquaculture. 5(3):25-44. doi:10.5897/IJFA12.062S25445

Effects of dietary inclusions of red beet and betaine on the acute stress response and muscle lipid peroxidation in rainbow trout

[EN] This study evaluates the effects of red beet (RB) and betaine on rainbow trout submitted to an acute stress challenge. A control diet was compared with four experimental diets in which red beet (14 and 28%) and betaine (0.9 and 1.63%) were incorporated in different concentrations according to a factorial design. Cortisol in plasma and fin, glucose and lactate plasma levels, and malondialdehide (MDA) in muscle were all measured before the stress challenge and 30 min and 6 and 12 h after the stress challenge as parameters to determine the diet effects. RB and betaine had no effect on cortisol, glucose, and MDA basal levels. However, lactate basal levels were significantly lower on fish fed with RB and betaine. Thirty minutes after the stress challenge, there was a significant increase in plasma and fin cortisol, glucose and lactate concentrations, although fish fed with diets containing RB and betaine showed significantly higher plasma cortisol values. MDA values of fish fed with 14% RB and 0.9% betaine were significantly higher than MDA values from fish fed with 28% RB and 1.63% betaine. After 6 and 12 h, plasma and fin cortisol and lactate levels recovered in a similar trend. Glucose plasma levels recovered in almost all groups 12 h after the stress. Also, MDA values recovered basal levels after 6 and 12 h. RB and betaine did not enhance the tolerance to the stress challenge compared to the control group, although the presence of these ingredients had no negative effect on any of the stress indicators.Pinedo-Gil, J.; Martín-Diana, AB.; Bertotto, D.; Sanz-Calvo, MÁ.; Jover Cerda, M.; Tomas-Vidal, A. (2018). Effects of dietary inclusions of red beet and betaine on the acute stress response and muscle lipid peroxidation in rainbow trout. Fish Physiology and Biochemistry. 44(3):939-948. doi:10.1007/s10695-018-0483-3S939948443Aluru N, Vijayan MM (2006) Aryl hydrocarbon receptor activation impairs cortisol response to stress in rainbow trout by disrupting the rate limiting steps in steroidogenesis. Endocrinology 147:1895–1903Ashley PJ (2007) Fish welfare: current issues in aquaculture. Appl Anim Behav Sci 104:199–235Barton BA, Iwama GK (1991) Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Annu Rev Fish Dis 1:3–26Bertotto D, Poltronieri C, Negrato E, Majolini D, Radaelli G, Simontacchi C (2010) Alternative matrices for cortisol measurement in fish. Aquac Res 41:1261–1267Bertotto D, Poltronieri C, Negrato E, Richard J, Pascoli F, Simontacchi C, Radaelli G (2011) Whole body cortisol and expression of HSP70, IGF-I and MSTN in early development of sea bass subjected to heat shok. Gen Comp Endocrinol 174:44–50Chagas EC, Val AL (2006) Ascorbic acid reduces the effects of hypoxia on the Amazon fish tambaqui. J Fish Biol 69:608–612Cui XJ, Zhou QC, Liang HO, Yang J, Zhao LM (2010) Effects of dietary carbohydrate sources on the growth performance and hepatic carbohydrate metabolic enzyme activities of juvenile cobia (Rachycentron canadum Linnaeus.) Aquac Res 42:99–107Dabrowski K, Lee KJ, Guz L, Verlhac V, Gabaudan J (2004) Effects of dietary ascorbic acid on oxygen stress (hypoxia or hyperoxia), growth and tissue vitamin concentration in juvenile rainbow trout (Oncorhynchus mykiss). Aquaculture 233:383–392Enes P, Panserat S, Kaushik S, Oliva-Teles A (2006) Rapid metabolic adaptation of European sea beass (Dicentrarchus labrax) juveniles fed different carbohydrate sources after heat shock stress. Comp Biochem Physiol A 145:73–81Fast MD, Hosoya S, Johnson SC, Alfonso LOB (2008) Cortisol response and immune-related effects of Atlantic salmon (Salmo salar Linnaeus) subjected to short- and long-term stress. Fish Shellfish Immunol 24:194–204Francis G, Makkar HPS, Becker K (2001) Antinutritional factors present in plant-derived alternate fish feed ingredients and their effects in fish. Aquaculture 199:197–227Ganessan B, Anandan R, Lakshmanan PT (2011) Studies on the protective effects of betaine against oxidative damage during experimentally induced restraint stress in Wistar albino rats. Cell Stress Chaperones 16:641–652Gesto M, López-Patiño MA, Hernández J, Soengas JL, Míguez JM (2013) The response of brain serotonergic and dopaminergic systems to an acute stressor in rainbow trout: a time course study. J Exp Biol 216:4435–4442Gesto M, López-Patiño MA, Hernández J, Soengas JL, Míguez JM (2015) Gradation of the stress response in rainbow trout exposed to stressors of different severity: the role of brain serotonergic and dopaminergic systems. J Neuroendocrinol 27:131–141Hemre GI, Mommsen TP, Krogdahl Å (2002) Carbohydrates in fish nutrition: effects on growth, glucose metabolism and heptic enzymes. Aquac Nutr 8:175–194Ings JS, Vijayan MM, Servos MR (2012) Tissue-specific metabolic changes in response to an acute handling disturbance in juvenile rainbow trout exposed to municipal wastewater effluent. Aquat Toxicol 108:53–59Janssens PA, Waterman J (1988) Hormonal regulation of gluconeogenesis and glycogenolysis in carp (Cyprinus carpio) liver pieces cultured in vitro. Comp Biochem Physiol 91A:451–457Jeney G, Galeotti M, Volpatti D, Anderson DP (1997) Prevention of stress in rainbow trout (Oncorhynchus mykiss) fed diets containing different doses of glucan. Aquaculture 154:1–15Kaplan LA, Pesce AJ (1984) Clinical chemistry: theory, analysis and correlation. Mosby, St. Louis, pp 1032–1036Krogdahl Å, Sundby A, Olli JJ (2004) Atlantic salmon (Salmon salar) and rainbow trout (Oncorhynchus mykiss) digest and metabolize nutrients differently. Effects of water salinity and dietary starch levels. Aquaculture 229:335–360Kujala TS, Vienola MS, Klika KD, Loponen JM, Pihlaja K (2002) Betalain and phenolic composition of four beetroot (Beta vulgaris) cultivars. Eur Food Res Technol 214:505–510Kumar N, Jadhao SB, Chandan NK, Kumar K, Jha AK, Bhushan S, Kumar S, Rana RS (2012) Dietary choline, betaine and lecithin mitigate endosulfan-induced stress in Labeo rohita fingerlings. Fish Physiol Biochem 38:989–1000Leveelahti L, Rytkönen KT, Renshaw GMC, Nikinmaa M (2014) Revisiting redox-active antioxidant defences in response to hypoxic challenge in both hypoxia-tolerant and hypoxia-sensitive fish species. Fish Physiol Biochem 40:183–191Lushchak VI, Bagnyukova TV, Lushchak OV, Storey JM, Storey KB (2005) Hypoxia and recovery perturb free radical processes and antioxidant potential in common carp (Cyprinus carpio) tissues. Int J Bichem Cell Biol 37:1319–1330Lushchak VI, Bagnyukova TV (2006) Temperature increase results in oxidative stress in goldfish tissues. 1. Indices of oxidative stress. Comp Biochem Physiol C 143:30–35Madaro A, Olsen RE, Kristiansen TS, Ebbeson LOE, Nilsen TO, Flik G, Gorissen M (2015) Stress in Atlantic salmon: response to unpredictable chronic stress. J Exp Biol 218:2538–2550Ming J, Xie J, Xu P, Ge X, Liu W, Ye J (2012) Effects of emodin and vitamin C on growth performance, biochemical parameters and two HSP70s mRNA expression of Wuchang bream (Megalobrama amblycephala Yih) under high temperature stress. Fish Shellfish Immunol 32:651–661Mommsen TP, Vijayan MM, Moon TW (1999) Cortisol in teleosts: dynamics, mechanisms of action, and metabolic regulation. Rev Fish Biol Fish 9:211–268Montero D, Tort L, Robaina L, Vergara JM, Izquierdo MS (2001) Low vitamin E in diet reduces stress resistance of gilthead seabream (Sparus aurta) juveniles. Fish Shellfish Immunol 11:473–490Ortuño J, Esteban MA, Meseguer J (2003) Effect of dietary intake of vitamins C and E on the stress response of gilthead seabream (Sparus aurata L.) Fish Shellfish Immunol 14:145–156Øverli Ø, Sørensen C, Kiessling A, Pottinger TG, Gjøen HM (2006) Selection for improved stress tolerance in rainbow trout (Oncorhynchus Mykiss) leads to reduced feed waste. Aquaculture 261:776–781Pérez-Jiménez A, Peres H, Rubio VC, Oliva-Teles A (2012) The effect of hypoxia on intermediary metabolism and oxidative status in gilthead sea bream (Sparus aurata) fed on diets supplemented with methionine and white tea. Comp Biochem Physiol C 155:506–516Pichavant K, Maxime V, Thébault MT, Ollivier H, Garnier JP, Bousquet B, Diouris M, Boeuf G, Nonnotte G (2002) Effects of hypoxia and subsequent recovery on turbot (Scophthalmus maximus): hormonal changes and anaerobic metabolism. Mar Ecol Prog Ser 225:275–285Pinedo-Gil J, Tomás-Vidal A, Larrán-García AM, Tomás-Almenar C, Jover-Cerdá M, Sanz-Calvo MA, Martín-Diana AB (2017a) Enhancement of quality of rainbow trout (Oncorhynchus mykiss) flesh incorporating barley on diet without negative effect on rearing parameters. Aquacult Int 25:1005–1023. https://doi.org/10.1007/s10499-016-0091-0Pinedo-Gil J, Tomás-Vidal A, Jover-Cerdá M, Tomás-Almenar C, Sanz-Calvo MA, Martín-Diana AB (2017b) Red beet and betaine as ingredients in diets of rainbow trout (Oncorhynchus mykiss): effects on growth performance, nutrient retention and flesh quality. Arch Anim Nutr 71:486–505. https://doi.org/10.1080/1745039X.2017.1391503Rabeh NM (2015) Effect of red beetroot (Beta vulgaris L.) and its fresh juice against carbon tetrachloride induced hepatotoxicity in rats. World Appl Sci J 33(6):931–938Rollo A, Sulpizio R, Nardi M, Silvi S, Orpianesi C, Caggiano M, Cresci A, Carnevalli O (2006) Live microbial feed supplement in aquaculture for improvement of stress tolerance. Fish Physiol Biochem 32:167–177Sadoul B, Leguen I, Colson V, Friggens NC, Prunet P (2015) A multivariate analysis using physiology and behaviour to characterize robustness in two isogenic lines of rainbow trout exposed to a confinement stress. Physiol Behav 140:139–147Tan Q, Xie S, Zhu X, Lei W, Yang Y (2006) Effect of dietary carbohydrates sources on growth performance and utilization for gibel carp (Carassius auratus) and Chinese longsnout catfish (Leiocassis Longirostris Günther). Aquac Nutr 12:61–70Tintos A, Míguez JM, Mancera JM, Soengas JL (2006) Development of a microtitre plate indirect ELISA for measuring cortisol in teleosts, and evaluation of stress responses in rainbow trout and gilthead sea bream. J Fish Biol 68:251–263Van Anholt RD, Spanings FAT, Koven WM, Nixon O, Wendelaar Bonga SE (2004) Arachidonic acid reduces the stress response of gilthead seabream, Sparus aurata L. J Exp Biol 207:3419–3430Virtanen E (1995) Piecing together the betaine puzzle. Feed Min 3:12–17Wu XY, Liu YJ, TIan LX, Mai KS, Yang HJ (2007) Utilization of several different carbohydrate sources by juvenile yellowfin seabream (Sparus latus). J Fish China 31(4):463–471Yoshida Y, Itoh N, Hayakawa M, Piga R, Cynshi O, Jishage K, Niki E (2005) Lipid peroxidation induced by carbon tetrachloride and its inhibition by antioxidant as evaluated by an oxidative stress marker, HODE. Toxicol Appl Pharmacol 208:87–97Zeng L, Wang YH, Ai CX, Zheng JL, Wu CW, Cai R (2016) Effects of β-glucan on ROS production and energy metabolism in yellow croaker (Pseudosciaena crocea) under acute hypoxic stress. Fish Physiol Biochem 42:1395–1405Zolderdo AJ, Algera DA, Lawrence MJ, Gilmour KM, Fast MD, Thuswaldner J, Willmore WG, Cooke SJ (2016) Stress, nutrition and parental care in a teleost fish: exploring mechanisms with supplemental feeding and cortisol manipulation. J Exp Biol 219:1237–124

Fish oil substitution by soybean oil in Diplodus puntazzo: Performance fatty acid profile and liver histology

[EN] The present study was performed to determine the effect of soybean oil on the performance and liver histology in sharpsnout sea bream. Four experimental diets were formulated containing 0%, 25%, 50% and 75% of soybean oil substituting of fish oil. Fish weighing 35 g were fed for 84 days. Increasing the level of soybean oil had no significant effects on growth and feed efficiency parameters. Biometrics, body composition, protein- and energy- efficiency were not affected by the fish oil replacement. Muscle and liver fatty acids reflected fish oil substitution. Moreover, histology did not show statistical differences among treatments.This research was supported by grants from the Planes Nacionales de Acuicultura (JACUMAR) in Spain.Nogales Mérida, S.; Martínez-Llorens, S.; Moñino López, AV.; Jover Cerda, M.; Tomas-Vidal, A. (2017). Fish oil substitution by soybean oil in Diplodus puntazzo: Performance fatty acid profile and liver histology. Journal of Applied Aquaculture. 29(1):46-61. doi:10.1080/10454438.2016.1274933S4661291Association of Official Analytical Chemists (AOAC). 1990. Official methods of analysis, 15th ed., p. 1298. Arlington, VA: Author.Caballero, M. J., Izquierdo, M. S., Kjorsvik, E., Fernandez, A. J., & Rosenlund, G. (2004). Histological alterations in the liver of sea bream, Sparus aurata L., caused by short- or long-term feeding with vegetable oils. Recovery of normal morphology after feeding fish oil as the sole lipid source. Journal of Fish Diseases, 27(9), 531-541. doi:10.1111/j.1365-2761.2004.00572.xCaballero, M. ., Obach, A., Rosenlund, G., Montero, D., Gisvold, M., & Izquierdo, M. . (2002). Impact of different dietary lipid sources on growth, lipid digestibility, tissue fatty acid composition and histology of rainbow trout, Oncorhynchus mykiss. Aquaculture, 214(1-4), 253-271. doi:10.1016/s0044-8486(01)00852-3Glencross, B., Hawkins, W., & Curnow, J. (2003). Evaluation of canola oils as alternative lipid resources in diets for juvenile red seabream, Pagrus auratus. Aquaculture Nutrition, 9(5), 305-315. doi:10.1046/j.1365-2095.2003.00257.xGrisdale-Helland, B., Ruyter, B., Rosenlund, G., Obach, A., Helland, S. ., Sandberg, M. ., … Røsjø, C. (2002). Influence of high contents of dietary soybean oil on growth, feed utilization, tissue fatty acid composition, heart histology and standard oxygen consumption of Atlantic salmon (Salmo salar) raised at two temperatures. Aquaculture, 207(3-4), 311-329. doi:10.1016/s0044-8486(01)00743-8Izquierdo, M. S., Obach, A., Arantzamendi, L., Montero, D., Robaina, L., & Rosenlund, G. (2003). Dietary lipid sources for seabream and seabass: growth performance, tissue composition and flesh quality. Aquaculture Nutrition, 9(6), 397-407. doi:10.1046/j.1365-2095.2003.00270.x(2007). Aquaculture Research, 38(1). doi:10.1111/are.2007.38.issue-1Martino, R. C., Cyrino, J. E. P., Portz, L., & Trugo, L. C. (2002). Performance and fatty acid composition of surubim (Pseudoplatystoma coruscans) fed diets with animal and plant lipids. Aquaculture, 209(1-4), 233-246. doi:10.1016/s0044-8486(01)00847-xMcFadzen, I. R. ., Coombs, S. ., & Halliday, N. . (1997). Histological indices of the nutritional condition of sardine, Sardina pilchardus (Walbaum) larvae off the north coast of Spain. Journal of Experimental Marine Biology and Ecology, 212(2), 239-258. doi:10.1016/s0022-0981(96)02755-4Nogales Mérida, S., Jover Cerdá, M., Martínez Llorens, S., & Tomás Vidal, A. (2011). A study of partial replacement of fish meal with sunflower meal on growth, amino acid retention, and body composition of sharpsnout seabream, Diplodus puntazzo (Actinopterygii: Perciformes: Sparidae). Acta Ichthyologica Et Piscatoria, 41(1), 47-54. doi:10.3750/aip2011.41.1.07Nogales-Mérida, S., Tomás-Vidal, A., Cerdá, M. J., & Martínez-Llorens, S. (2011). Growth performance, histological alterations and fatty acid profile in muscle and liver of sharp snout sea bream (Diplodus puntazzo) with partial replacement of fish oil by pork fat. Aquaculture International, 19(5), 917-929. doi:10.1007/s10499-010-9410-zNogales Mérida, S., Tomás-Vidal, A., Martínez-Llorens, S., & Jover Cerdá, M. (2010). Sunflower meal as a partial substitute in juvenile sharpsnout sea bream (Diplodus puntazzo) diets: Amino acid retention, gut and liver histology. Aquaculture, 298(3-4), 275-281. doi:10.1016/j.aquaculture.2009.10.025O’Fallon, J. V., Busboom, J. R., Nelson, M. L., & Gaskins, C. T. (2007). A direct method for fatty acid methyl ester synthesis: Application to wet meat tissues, oils, and feedstuffs. Journal of Animal Science, 85(6), 1511-1521. doi:10.2527/jas.2006-491Olsen, R. E., Tore Dragnes, B., Myklebust, R., & Ringø, E. (2003). Effect of soybean oil and soybean lecithin on intestinal lipid composition and lipid droplet accumulation of rainbow trout, Oncorhynchus mykiss Walbaum. Fish Physiology and Biochemistry, 29(3), 181-192. doi:10.1023/b:fish.0000045708.67760.43Peng, S., Chen, L., Qin, J. G., Hou, J., Yu, N., Long, Z., … Sun, X. (2008). Effects of replacement of dietary fish oil by soybean oil on growth performance and liver biochemical composition in juvenile black seabream, Acanthopagrus schlegeli. Aquaculture, 276(1-4), 154-161. doi:10.1016/j.aquaculture.2008.01.035Piedecausa, M. A., Mazón, M. J., García García, B., & Hernández, M. D. (2007). Effects of total replacement of fish oil by vegetable oils in the diets of sharpsnout seabream (Diplodus puntazzo). Aquaculture, 263(1-4), 211-219. doi:10.1016/j.aquaculture.2006.09.039Richard, N., Mourente, G., Kaushik, S., & Corraze, G. (2006). Replacement of a large portion of fish oil by vegetable oils does not affect lipogenesis, lipid transport and tissue lipid uptake in European seabass (Dicentrarchus labrax L.). Aquaculture, 261(3), 1077-1087. doi:10.1016/j.aquaculture.2006.07.021Roberts, R. J. 1981. Patología de los peces. In ed. M. Prensa, 366. Madrid, Spain.Ruyter, B., Moya-Falcón, C., Rosenlund, G., & Vegusdal, A. (2006). Fat content and morphology of liver and intestine of Atlantic salmon (Salmo salar): Effects of temperature and dietary soybean oil. Aquaculture, 252(2-4), 441-452. doi:10.1016/j.aquaculture.2005.07.014Snedecor, G., and W. Cochran. 1971. Statistical methods, p. 592. Ames, IA: Iowa State University Press.Turchini, G. M., Mentasti, T., Frøyland, L., Orban, E., Caprino, F., Moretti, V. M., & Valfré, F. (2003). Effects of alternative dietary lipid sources on performance, tissue chemical composition, mitochondrial fatty acid oxidation capabilities and sensory characteristics in brown trout (Salmo trutta L.). Aquaculture, 225(1-4), 251-267. doi:10.1016/s0044-8486(03)00294-1(2009). Reviews in Aquaculture, 1(1). doi:10.1111/raq.2009.1.issue-

Red beet and betaine as ingredients in diets of rainbow trout (Oncorhynchus mykiss): effects on growth performance, nutrient retention and flesh quality

[EN] The objective of the study was to evaluate the impact of different concentrations of dietary red beet and betaine on the growth performance and fish flesh quality of rainbow trout. Therefore, a control diet was compared with four diets in which two levels of red beet (14% and 28%) and betaine (0.9% and 1.63%) were incorporated in combination. The study was set up with an average body weight of 69 ± 2.2 g and finished when fish reached commercial weight (175¿250 g) after 105 d. The impact of the diets was studied based on the growth performance, biometric indexes, proximal composition, protein and fat retention efficiencies and apparent nutrient digestibility by fish reared on a recirculation system. Further estimates were the effect of red beet and betaine on the flesh proximate composition and quality of the final product (water activity, colour, texture, thiobarbituric acid reactive substances and sensory characteristics). Results showed that inclusion of 14% red beet and 0.9% betaine did not affect growth, nutritive or biometric parameters and nutrient retention when compared with the control diet. However, higher levels of red beet and betaine had negative effects on growth and nutritive parameters. The tested ingredients enhanced quality parameters regardless of the concentration used. After feeding the red beet and betaine, fish flesh showed lower water activity and better textural and colour properties than the control and also a dose-dependent effect on lipid oxidation was observed.This work has been co-funded with FEDER and INIA funds. Julia Pinedo has been granted with the FPI-INIA grant number 21 [call 2012, BOE-2012-13337].Pinedo-Gil, J.; Tomas-Vidal, A.; Jover Cerda, M.; Tomás-Almenar, C.; Sanz-Calvo, MÁ.; Martín-Diana, AB. (2017). Red beet and betaine as ingredients in diets of rainbow trout (Oncorhynchus mykiss): effects on growth performance, nutrient retention and flesh quality. Archives of Animal Nutrition. 71(6):486-505. doi:10.1080/1745039X.2017.1391503S48650571

A study of partial replacement of fish meal with sunflower meal on growth, amino acid retention, and body composition of sharpsnout seabream, Diplodus puntazzo (Actinopterygii: Perciformes: Sparidae)

Background. Our previous study demonstrated that sharpsnout seabream, Diplodus puntazzo (Walbaum, 1792), can be fed with up to 34.8% sunflower meal (SFM), with excellent results in growth parameters and feed efficiency. The aim of the current study was to test the replacement of fish meal with SFM in the diet formulation for sharpsnout seabream and to evaluate growth, nutritive parameters, amino acid retention, and body composition of the fish during the fattening period. Materials and methods. Sixteen baskets (300-L capacity), each with thirteen fish, were distributed in a recirculated saltwater system to allow four experimental diets containing 40% crude protein (CP) and 20% crude lipid (CL) with 0%, 11.7%, 23.5%, and 34.8% SFM partially replacing fish meal to be used in quadruplicate for this experiment. The fish were fed these diets ad libitum during the experiment, which lasted for 162 days. Growth, nutrition efficiency, biometrics, carcass composition, amino acid composition, and amino acid retention of the experimental fish were evaluated. Results. There were no statistical differences in the growth parameters among the treatments. However, fish fed a diet containing 34.8% SFM had the lowest feed intake (FI), lowest feed conversion ratio (FCR), and the highest protein efficiency ratio (PER). There were also no statistical differences in the biometric parameters although fish fed the diet containing 10% SFM had the lowest CP levels. Leucine was the only difference in the essential amino acid (EEA) profile with fish fed a diet containing 11.7% SFM having the lowest levels of leucine. Although there were fluctuations among the amino acid retentions, they were not statistically significant. Conclusion. SFM (up to 34.8%) can be included in the diets of sharpsnout seabream, thereby, replacing 27% of the fish meal without altering the fish growth.This research was supported by grants from the Planes Nacionales de Acuicultura (JACUMAR).Nogales Mérida, S.; Jover Cerda, M.; Martínez-Llorens, S.; Tomás Vidal, A. (2011). A study of partial replacement of fish meal with sunflower meal on growth, amino acid retention, and body composition of sharpsnout seabream, Diplodus puntazzo (Actinopterygii: Perciformes: Sparidae). Acta Ichthyologica et Piscatoria. 41(1):47-54. doi:10.3750/AIP2011.41.1.07S475441

Impact of Fishmeal Replacement in Diets for Gilthead Sea Bream (Sparus aurata) on the Gastrointestinal Microbiota Determined by Pyrosequencing the 16S rRNA Gene

[EN] Recent studies have demonstrated the impact of diet on microbiota composition, but the essential need for the optimization of production rates and costs forces farms and aquaculture production to carry out continuous dietary tests. In order to understand the effect of total fishmeal replacement by vegetable-based feed in the sea bream (Sparus aurata), the microbial composition of the stomach, foregut, midgut and hindgut was analysed using high-throughput 16S rDNA sequencing, also considering parameters of growth, survival and nutrient utilisation indices.A total of 91,539 16S rRNA filtered-sequences were analysed, with an average number of 3661.56 taxonomically assigned, high-quality sequences per sample. The dominant phyla throughout the whole gastrointestinal tract were Actinobacteria, Protebacteria and Firmicutes. A lower diversity in the stomach in comparison to the other intestinal sections was observed. The microbial composition of the Recirculating Aquaculture System was totally different to that of the sea bream gastrointestinal tract. Total fishmeal replacement had an important impact on microbial profiles but not on diversity. Streptococcus (p-value: 0.043) and Photobacterium (p-value: 0.025) were highly represented in fish fed with fishmeal and vegetable-meal diets, respectively. In the stomach samples with the vegetable diet, reads of chloroplasts and mitochondria from vegetable dietary ingredients were rather abundant. Principal Coordinate Analysis showed a clear differentiation between diets in the microbiota present in the gut, supporting the presence of specific bacterial consortia associated with the diet.Although differences in growth and nutritive parameters were not observed, a negative effect of the vegetable diet on the survival rate was determined. Further studies are required to shed more light on the relationship between the immune system and sea bream gastrointestinal tract microbiota and should consider the modulation of the microbiota to improve the survival rate and nutritive efficacy when using plant-based diets.Project Name: Aquaculture feed without fishmeal(SP20120603). Grant Numbers: 6.000,00. Funding Institution: Vicerrectorat d'Investigacio, Innovacio i Transferencia - Universitat Politecnica de Valencia. Author who received the funding: ATV. URL of funder: http://www.upv.es/entidades/VIIT/info/indexnormalc.html. The first author was supported by a contract-grant (Contrato Pre-doctoral del Programa para la Formacion de Personal Investigador - FPI) from Programa de Ayudas de Investigacion y Desarrollo (PAID) of Universitat Politecnica de Valencia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Estruch, G.; Collado Amores, MC.; Peñaranda, D.; Tomas-Vidal, A.; Jover Cerda, M.; Pérez-Martínez, G.; Martínez-Llorens, S. (2015). Impact of Fishmeal Replacement in Diets for Gilthead Sea Bream (Sparus aurata) on the Gastrointestinal Microbiota Determined by Pyrosequencing the 16S rRNA Gene. PLoS ONE. 10:1-22. doi:10.1371/journal.pone.0136389S1221

Mejora de las competencias gracias a la implantación de aprendizaje basado en proyectos en acuicultura

En el presente trabajo se muestra la experiencia de la aplicación del Aprendizaje Basada en proyectos (ABP) en la asignatura “Diseño y Gestión de instalaciones Piscícolas” de la titulación del Máster interuniversitario de Acuicultura de la Universitat Politécnica de València. El ABP consistió en el desarrollo de un proyecto de una piscifactoría, en el cual los alumnos debían integrar los conocimientos adquiridos en las asignaturas previas, permitiendo una visión más multidisciplinar de la asignatura y mejor adquisición de las competencias. Tanto desde el punto de vista del alumnado como del profesor, la implantación del ABP permitió un mayor desarrollo de las competencias, siendo la metodología docente valorada positivamente. Además, se mejoró la participación del alumnado en las actividades propuestas, dando como resultado una mejor calificando académica final. No obstante, se deben de mejorar algunos aspectos como es la planificación del tiempo y consolidar relaciones de integración o la interacción entre diferentes disciplinas/asignaturas de manera que tengan una mejor integración en el proyecto

Eficiencia de diferentes sistemas de evaluación en la asignatura de “Nutrición de especies acuícolas”

[EN] The subject of “Nutricion de especies acuícolas” is teach in in-person modality in the “Master Interuniversitario de Acuicultura, and in on-line modality in the “Curso de especialista universitario en Acuicultura”. Even if the assessment procedures and percentages of differents parts over the final grade are similar in both modalities, there are not equals the number of assessments facts. Keeping in mind the satisfactions surveis of the students over the assesments systems used, the results where higher for the on-line modality (9,4) than for the in-persone one (8,1). The higher sattisfaction of the students with the assessment system has a correspondence with better student performance, showing a higher percentage of student with grade over 9, 24,5% if compared with the 14,5% in the in-person modality. But similar percentage of students obtained a grade between 7 and 9 (50 and 54% respectivily)[ES] La asignatura de “Nutrición de especies acuícolas” se imparte de forma presencial en el Master Interuniversitario de Acuicultura y formato on-line en el Curso de Especialista universitario en Acuicultura. Aunque el tipo de evaluación, así como los porcentajes que representan en la nota final son similares en ambas modalidades, no es el número de actos realizados. Teniendo en cuenta las encuestas de satisfacción de los alumnos en el apartado de los sistemas de evaluación empleados fueron más elevadas en la asignatura on-line (9,4), que en la asignatura de docencia presencial (8,1). La mayor satisfacción de los alumnos con el sistema de evaluación propuesta se ha traducido en un mayor rendimiento académico, presentando un elevado porcentaje de alumnos con una nota superior a 9, un 24.5% frente a un 14,5% en la asignatura presencial, siendo el porcentaje de notables muy similar en ambas asignaturas (50 y 54%, respectivamente)Tomás Vidal, A.; Jauralde García, I.; Martínez Llorens, S.; Sánchez Peñaranda, D.; Jover Cerdá, M. (2015). Eficiencia de diferentes sistemas de evaluación en la asignatura de “Nutrición de especies acuícolas”. En In-Red 2015 - CONGRESO NACIONAL DE INNOVACIÓN EDUCATIVA Y DE DOCENCIA EN RED. Editorial Universitat Politècnica de València. https://doi.org/10.4995/INRED2015.2015.1625OC

Aprendizaje basado en proyectos: una propuesta eficaz para el desarrollo de las competencias en el master de Acuicultura

[ES] En el presente trabajo se muestra una experiencia de la aplicación del Aprendizaje Basada en proyectos en la titulación del Master interuniversitario de Acuicultura de la Universitat Politécnica de València. El ABPr se centró en el desarrollo de un proyecto de una piscifactoría, en el cual los alumnos pudieran adquirir una visión en conjunto de las asignaturas impartidas en el máster, fomentando la conexión entre ellas, reforzando, por tanto, el carácter interdisciplinar del proyecto. Para evaluar la metodología se realizó cuestionario cuantitativo y otro cualitativo con el objetivo de conocer las opiniones de los alumnos. Los alumnos han valorado positivamente la metodología, destacando la importancia del proyecto desarrollado para su formación profesional, así como también se valoró sustancialmente que el trabajo desarrollado en grupo cumplió sus expectativas en cuanto a la labor de los docentes. Aunque no se observaron valoraciones negativas de la metodología desarrollada, se deben de mejorar algunos aspectos como la planificación del tiempo y consolidar relaciones de integración o la interacción entre diferentes disciplinas/asignaturas.[EN] This work shows an of Project Based Learning experience on Interuniversity Master of Aquaculture degree at the Universitat Politécnica de València. The ABPr focused on the development of a fish farm project, in which the students could acquire a combined vision of the subjects taught during the master's degree, promoting the connection between subjects, thus reinforcing the interdisciplinary character of the project. To evaluate the methodology, a quantitative and a qualitative survey was conducted with the aim to know the students opinions. The students have positively valued the methodology, highlighting the importance of the project developed for their professional training, as well as the fact that the project carried out as teamwork met their expectations regarding the teachers´work. Although no negative evaluations about the developed methodology were observed, some aspects such as time scheduling, and the integration of additional subjects/ disciplines should be improved.Martínez-Llorens, S.; Jauralde García, I.; S. Peñaranda, D.; Tomás-Vidal, A.; Jover Cerdá, M. (2021). Aprendizaje basado en proyectos: una propuesta eficaz para el desarrollo de las competencias en el master de Acuicultura. En IN-RED 2020: VI Congreso de Innovación Educativa y Docencia en Red. Editorial Universitat Politècnica de València. 208-2017. https://doi.org/10.4995/INRED2020.2020.11988OCS208201