6 research outputs found
Genetically Engineered Crops: Importance of Diversified Integrated Pest Management for Agricultural Sustainability
As the global population continues to expand, utilizing an integrated approach to pest management will be critically important for food security, agricultural sustainability, and environmental protection. Genetically engineered (GE) crops that provide protection against insects and diseases, or tolerance to herbicides are important tools that complement a diversified integrated pest management (IPM) plan. However, despite the advantages that GE crops may bring for simplifying the approach and improving efficiency of pest and weed control, there are also challenges for successful implementation and sustainable use. This paper considers how several GE traits, including those that confer protection against insects by expression of proteins from Bacillus thuringiensis (Bt), traits that confer tolerance to herbicides, and RNAi-based traits that confer resistance to viral pathogens, can be key elements of a diversified IPM plan for several different crops in both developed and developing countries. Additionally, we highlight the importance of community engagement and extension, strong partnership between industry, regulators and farmers, and education and training programs, for achieving long-term success. By leveraging the experiences gained with these GE crops, understanding the limitations of the technology, and considering the successes and failures of GE traits in IPM plans for different crops and regions, we can improve the sustainability and versatility of IPM plans that incorporate these and future technologies
Historia y situación actual de la producción de frijol en los países latinoamericanos afectados por geminivirus transmitidos por mosca blanca: Brasil
América del Sur, Brasil.Centro Internacional de Agricultura Tropical (CIAT)UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Estación Experimental Agrícola Fabio Baudrit Moreno (EEAFBM
Development of Plasmid DNA Reference Material for the Quantification of Genetically Modified Common Bean Embrapa 5.1
The
genetically modified (GM) common bean Embrapa 5.1 was recently
approved for commercialization. The reliable detection and quantification
of GM organisms is strongly dependent on validated methods as well
as calibration systems. This work presents the development of a calibrant
plasmid for Embrapa 5.1 common bean detection. The reaction parameters
were determined and compared for both the plasmid DNA (pDNA) and the
genomic DNA (gDNA). PCR efficiencies for pDNA were 81% for the construction-specific
assays and 76% for the taxon-specific assay, whereas for gDNA efficiencies
were 94 and 93%, respectively. The limits of detection (LOD) in both
qPCR assays were 10<sup>2</sup> and 10<sup>3</sup> copies of gDNA
and pDNA per PCR reaction, respectively. This is sufficient to detect
0.067 and 0.67% of GM common bean in 100 ng of DNA, respectively,
which is in agreement with detecting the 1% GM content required by
the Brazilian legislation
Diversidad genética en los geminivirus del frijol transmitidos por mosca blanca
Programa Cooperativo Regional de Frijol para Centroamérica, México y el Caribe (PROFRIJOL)Cooperación Suiza para el Desarrollo (COSUDE)Centro Internacional de Agricultura Tropical (CIAT)UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Estación Experimental Agrícola Fabio Baudrit Moreno (EEAFBM