23 research outputs found
Dexpramipexole Is Ineffective in Two Models of ALS Related Neurodegeneration
<div><p>Treatment options for people living with amyotrophic lateral sclerosis (ALS) are limited and ineffective. Recently, dexpramipexole (RPPX) was advanced into human ALS clinical trials. In the current studies, we investigated RPPX in two parallel screening systems: 1) appropriately powered, sibling-matched, gender-balanced survival efficacy screening in high-copy B6-SJL-SOD1<sup>G93A</sup>/Gur1 mice, and 2) high-content neuronal survival screening in primary rat cortical neurons transfected with wild-type human TDP43 or mutant human TDP43. In both cases, we exposed the test systems to RPPX levels approximating those achieved in human Phase II clinical investigations. In SOD1<sup>G93A</sup> mice, no effect was observed on neuromotor disease progression or survival. In primary cortical neurons transfected with either mutant or wild-type human TDP43, a marginally significant improvement in a single indicator of neuronal survival was observed, and only at the 10 µM RPPX treatment. These systems reflect both mutant SOD1- and TDP43-mediated forms of neurodegeneration. The systems also reflect both complex non-cell autonomous and neuronal cell autonomous disease mechanisms. The results of these experiments, taken in context with results produced by other molecules tested in both screening systems, do not argue positively for further study of RPPX in ALS.</p></div
Steady-state dexpramipexole concentrations in SOD1<sup>G93A</sup> mice receiving drug ad libitum in drinking water.
<p>Three males and three females in each group received 0.179, 0.9, or 1.79 mg/mL dexpramipexole dihydrochloride in the drinking water. Mean ± standard error of the mean concentration in plasma and spinal cord at steady-state after 14 days of treatment is shown at each dose level. A concentration of 1.19 mg/mL in the drinking water was chosen for the survival study in SOD1G93A mice to emulate steady-state concentrations attained by the 300 mg/d group reported in the human clinical trial (1).</p
Dexpramipexole Stability in Drinking Water over One Week by HPLC.
<p>Dexpramipexole Stability in Drinking Water over One Week by HPLC.</p
Cortical Neuron Survival Data, TDP43-EGFP with RPPX Treatment.
<p>Cortical Neuron Survival Data, TDP43-EGFP with RPPX Treatment.</p
Kaplan-Meier survival plot for age at death in SOD1<sup>G93A</sup> mice.
<p>Left panel shows data from all animals (All); middle and right panels show data from females (F) and males (M), respectively. Vehicle control (CTRL) animals received 0.22 micron filtered animal facility drinking water. Dexpramipexole-treated (DRUG) animals received daily 200 mg/kg/day dexpramipexole (1.19 g/L) in filtered drinking water. Results of statistical analysis for these data are given in the lower portion of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0091608#pone-0091608-t004" target="_blank">Table 4</a>.</p
Cortical Neuron Survival Data, TDP43<sup>A315T</sup>-EGFP with RPPX Treatment.
<p>Cortical Neuron Survival Data, TDP43<sup>A315T</sup>-EGFP with RPPX Treatment.</p
Time-to-Event Analysis for Onset of Neurological Symptoms and for Survival Duration.<sup>1–3</sup>
<p>Testing Terms: In Kaplan-Meier analysis the Log-Rank test places more weight on later event times; the Wilcoxon test places more weight on early event times and is the optimum rank test if the error distribution is logistic. Prob > ChiSq lists the probability of obtaining, by chance alone, a Chi-square value greater than the one computed if the time-to-event functions are the same for all groups. In Cox proportional hazards analysis the Effect Likelihood Test is the likelihood-ratio Chi-square test on the null hypothesis that the parameter estimate for the Treatment covariate is zero (no effect of treatment). Testing term descriptions are taken from the JMP 10.0.0 Help file. A neurological score of two in both hind limbs is taken to be the definitive onset of neurological symptoms. Animals that did not reach a neurological severity score of 2 prior to termination of the experiment at 180 days of age were right-censored in the onset analysis. Animals that did not die by 180 days of age were right-censored in the survival analysis.</p><p>Time-to-Event Analysis for Onset of Neurological Symptoms and for Survival Duration.<sup>1–3</sup></p
Cortical Neuron Survival Data, TDP43<sup>M337V</sup>-EGFP with RPPX Treatment.
<p>Cortical Neuron Survival Data, TDP43<sup>M337V</sup>-EGFP with RPPX Treatment.</p
RRPX has little effect on neuronal survival in TDP43 overexpression model of ALS.
<p>(A–D) Kaplan-Meier cumulative hazard plots for neurons treated with RPPX at doses indicated for neurons transfected with mApple and (A) EGFP, (B) wildtype TDP43-EGFP, (C) TDP43<sup>A315T</sup>-EGFP, (D) TDP43<sup>M337V</sup>-EGFP. (E) Hazard ratios relative to control (EGFP, no RPPX) for all conditions shown above. Data for each condition are pooled from n = 2–5 biological replicates.</p
Daily average body weight change and neurological severity score in SOD1<sup>G93A</sup> mice.
<p>Group average change from initial body weight (top panels) and neurological disease severity score (bottom panels) are compared over time from age at study start to age 180 days. Left panels show data from all animals (All); middle and right panels show data from females (F) and males (M), respectively. Vehicle control (CTRL) animals received 0.22 micron filtered animal facility drinking water. Dexpramipexole-treated (DRUG) animals received daily 200 mg/kg/day dexpramipexole (1.19 g/L) in filtered drinking water. Because animals died at different ages, group means were computed after carrying forward last values for each animal through 180 days of age. Results of statistical analysis for summary measures taken from these data are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0091608#pone-0091608-t002" target="_blank">Table 2</a>.</p