A-G.

<p><b>A)</b> TBL in the submandibular lymph node of an affected pig. Bar, 1cm. B) TBL in the spleen of an affected pig. Bar, 1cm. C) Microscopic image of a TBL lesions in the lymph node of an affected animal showing a profuse infiltrate of degenerated neutrophils. HE. Bar, 200μm. D) Clustered epithelioid macrophages surrounded by lymphocytes and erythrocytes in a stage I granuloma in the liver. HE. Bar, 50μm. E) Coalescent stage II granulomas in the lymph node of a pig showing epithelioid macrophages completely enclosed by a thin capsule, with peripheral infiltration of scattered lymphocytes. HE. Bar, 100μm. F) Stage III granuloma with a central necrotic core, partially mineralized, surrounded by a dense connective tissue capsule infiltrated by lymphocytes and scattered neutrophils. HE. Bar, 100μm. G) Thickly encapsulated, large, irregular, multicentric granulomas with prominent caseous necrosis and multifocal islands of mineralization (stage IV granulomas). HE. Bar, 500μm.</p

Microorganisms isolated from lesions.

<p>^<b>a</b><i>Rhodococcus boritolerans</i>, <i>Dietzia timorensis</i>, <i>Pseudoclavibacter</i> spp, <i>Brevibacterium</i> spp and <i>Actinomyces masicol</i> (1 isolate/each)</p><p>^b<i>Streptococcus</i> spp. (3 isolates), <i>S</i>. <i>mitis</i>, <i>S</i>. <i>rattus</i> and <i>S</i>. <i>bovis</i> (1 isolate/each)</p><p>^c<i>E</i>. <i>faecium</i> (8 isolates) <i>E</i>. <i>durans</i> (3 isolates), <i>E</i>. <i>faecalis</i> (6 isolates), <i>E</i>. <i>gallinarum</i> and <i>E</i>. <i>avium</i> (1 isolate/each)</p><p>^d<i>C</i>. <i>maltaromaticum</i> (16 isolates) and <i>C</i>. <i>divergens</i> (1 isolate)</p><p>^e<i>A</i>. <i>urinae</i> (7 isolates), <i>A</i>. <i>viridans</i> (4 isolates) and <i>A nurinaequi</i> (2 isolates)</p><p>^f<i>S</i>. <i>sciuri</i>, <i>S</i>. <i>xylosus</i> (2 isolates/each), <i>Staphylococcus</i> spp., <i>S</i>. <i>aureus</i> and <i>S</i>. <i>haemolyticus</i> (1 isolate/each)</p><p>^<b>g</b><i>Leuconostoc</i> spp. (4 isolates), <i>Escherichia coli</i> (2 isolates) <i>Mezorhizobium</i> spp., <i>Halospirulina</i> spp., <i>Glanulicatella</i> spp. and <i>Lactococcus lactis</i> (1 isolate/each)</p><p>Microorganisms isolated from lesions.</p

Type of lesions identified from samples.

<p>^aSLN: submandibular lymph nodes.</p><p>Type of lesions identified from samples.</p

Distribution of granulomas per examined organ and stage of development.

<p>^aSLN: submandibular lymph nodes.</p><p>Distribution of granulomas per examined organ and stage of development.</p

Frequency of detected microorganisms and type of lesions identified at individual level.

<p>^aGranulomatous and pyogranulomatous lesions detected in the same animal</p><p>^bNecrotic or calcified foci (5/15) and other lesions or no lesions (10/15)</p><p>Frequency of detected microorganisms and type of lesions identified at individual level.</p

Frequency of detection of microorganisms from TBL within the examined organs.

<p>^aSLN: submandibular lymph nodes.</p><p>Frequency of detection of microorganisms from TBL within the examined organs.</p