Intrauterine repair of gastroschisis in fetal rabbits

Objective: Infants with gastroschisis (GS) still face severe morbidity. Prenatal closure may prevent gastrointestinal organ damage, but intrauterine GS repair (GSR) has not been established yet. Methods: In New Zealand White rabbits we developed and compared GS versus GSR: creation of GS was achieved by hysterotomy, right-sided laparotomy of the fetus and pressure on the abdominal wall to provoke evisceration. GSR was accomplished by careful reposition of eviscerated organs and a running suture of the fetal abdominal wall. For study purposes, 18 animals were divided equally into 3 groups: GS, GS with GSR after 2 h, and unmanipulated controls (C). Vitality was assessed by echocardiography. After 5 h all animals were sacrificed. Results: GSR inflicted no increased mortality, because all fetuses survived GS or GS with GSR. All fetuses with GS demonstrated significant evisceration of abdominal organs. In contrast, the abdominal wall of the fetuses from GSR was intact. Conclusion:The present animal model demonstrated the technical feasibility and success of an intrauterine repair of GS for the first time. However, further long-term studies (leaving GS and GSR in utero for several days) will be necessary to compare survival rates and intestinal injury, motility or absorption. The clinical application of GSR in utero remains a vision so far. Copyright (C) 2003 S. Karger AG, Basel

Development of a Modular Biosensor System for Rapid Pathogen Detection

Progress in the field of pathogen detection relies on at least one of the following three qualities: selectivity, speed, and cost-effectiveness. Here, we demonstrate a proof of concept for an optical biosensing system for the detection of the opportunistic human pathogen Pseudomonas aeruginosa while addressing the abovementioned traits through a modular design. The biosensor detects pathogen-specific quorum sensing molecules and generates a fluorescence signal via an intracellular amplifier. Using a tailored measurement device built from low-cost components, the image analysis software detected the presence of P. aeruginosa in 42 min of incubation. Due to its modular design, individual components can be optimized or modified to specifically detect a variety of different pathogens. This biosensor system represents a successful integration of synthetic biology with software and hardware engineering

Functional conservation of the Drosophila hybrid incompatibility gene Lhr

<p>Abstract</p> <p>Background</p> <p>Hybrid incompatibilities such as sterility and lethality are commonly modeled as being caused by interactions between two genes, each of which has diverged separately in one of the hybridizing lineages. The gene <it>Lethal hybrid rescue </it>(<it>Lhr</it>) encodes a rapidly evolving heterochromatin protein that causes lethality of hybrid males in crosses between <it>Drosophila melanogaster </it>females and <it>D. simulans </it>males. Previous genetic analyses showed that hybrid lethality is caused by <it>D. simulans Lhr </it>but not by <it>D. melanogaster Lhr</it>, confirming a critical prediction of asymmetry in the evolution of a hybrid incompatibility gene.</p> <p>Results</p> <p>Here we have examined the functional properties of <it>Lhr </it>orthologs from multiple Drosophila species, including interactions with other heterochromatin proteins, localization to heterochromatin, and ability to complement hybrid rescue in <it>D. melanogaster</it>/<it>D. simulans </it>hybrids. We find that these properties are conserved among most <it>Lhr </it>orthologs, including <it>Lhr </it>from <it>D. melanogaster</it>, <it>D. simulans </it>and the outgroup species <it>D. yakuba</it>.</p> <p>Conclusions</p> <p>We conclude that evolution of the hybrid lethality properties of <it>Lhr </it>between <it>D. melanogaster </it>and <it>D. simulans </it>did not involve extensive loss or gain of functions associated with protein interactions or localization to heterochromatin.</p

sequ-into demo/test data

# sequ-into demo dataThis archive/folder contains the demo data for sequ-into.Most relevant workflows/use-cases are explorable with these data. Obviously the live-setup can not be fully simulated with these data, as this would require a lab, a sequencer, material, etc.However, both the retrospective analysis and the use of temporary fastq-files (basecalled, but in MinKNOW tmp-folder) from MinKNOW can be explored.## Use-cases1. The [first use-case](1_fastq_retro/README.MD) describes the usage of sequ-into with a FASTQ file.2. The [second use-case](2_fast5_single_retro/README.MD) describes the usage of sequ-into with (older) single-FAST5-Files (e.g. one read per FAST5 file). This use-case is particularly useful for a retrospective analysis.3. The [third use-case](3_fast5_live_tmp/README.MD) demonstrates the usage of FASTQ-TMP-Files with sequ-into.4. The [fourth use-case](4_fast5_multi_retro/README.MD) describes the usage of sequ-into with modern multi-FAST5 files (e.g. multiple reads per FAST5 file). This use-case is particularly useful for retrospective analyses of recent MinION data.5. The [fifth use-case](5_fast5_single_retro_extension/README.MD) extends the second use-case by adding more reads. This simulates the progress over time for a real-time analysis.## Data UsageThe included read data (FASTQ/FAST5/tmp-files) may only be used for testing sequ-into and remain property of the designated authors (under Data origin in each use-case). This data may be shared under CC BY NC 3.0 license.The respective authors gave consent for using these files as part of this demo data collection only.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Personalized production, non-genomic engineering, and time-resolved proteomics of therapeutic phages for biohazardous and multidrug-resistant bacteria

masurca.config =&gt; assembly config filep3.blastvog.enhanced.3.embl =&gt; final assembly of P3 phage with annotation (embl-file format - *not* genbank!)p3.comp.pdf|png =&gt; genome duplication analysisp3.guppy.fastq.gz =&gt; MinION readsp3.noecoli.guppy.fastq.gz =&gt; MinION reads (E. coli reads removed)p3.r.1|2.fq.gz =&gt; trimmed/E. coli removed reads from Illumina sequencingP3_S2* =&gt; original Illumina readsTHIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Western Star, 1911-10-04

The Western Star began publication on Newfoundland's west coast on 4 April 1900, appearing weekly with brief semiweekly periods up to 1952, when it became a daily. As of 17 April 2019 it continues as a free weekly community paper