Reply to Comment on "Reevaluation of the parton distribution of strange quarks in the nucleon"

A Comment on the recently published reevaluation of the polarization-averaged parton distribution of strange quarks in the nucleon using final data on the multiplicities of charged kaons in semi-inclusive deep-inelastic scattering is reviewed. Important features of the comparison of one-dimensional projections of the multidimensional HERMES data are pointed out. A test of the leading-order extraction of xS(x) using the difference between charged-kaon multiplicities is repeated. The results are consistent with leading-order predictions within the uncertainties in the input data, and do not invalidate the earlier extraction of xS(x).Comment: Reply Comment to arXiv:1407.372

Ramsar Policy Brief No. 5. Restoring drained peatlands: A necessary step to achieve global climate goals

Peatlands cover about 400 million hectares (ha), or 3% of the land surface of our planet. Yet they store more carbon, more effectively and for longer periods, than any other ecosystem on land. Intact peatlands also provide essential ecosystem services such as regulating water cycles, purifying water, and supporting a wealth of biodiversity. Since peat is hidden below ground, it is often unrecognised and can be damaged unknowingly. New, large peatland areas are still being discovered including forest-covered peatlands in the tropics. Around 50 million ha of peatlands globally are currently drained and have been transformed to grazing land, forestry land and cropland, used for peat extraction or impacted by infrastructure. These drained peatlands are responsible for approximately 4% (2 Gt CO2 -eq/year) of all anthropogenic greenhouse gas emissions. Achieving the climate goals of the Paris Agreement requires protection of all remaining intact peatland and rapid restoration of almost all drained peatlands. This will also contribute to delivering the Sustainable Development Goals (SDGs), in particular SDG 6, Target 6.6, on protecting and restoring water related ecosystems and SDG 15, Targets 15.1, on conservation, restoration and sustainable use of terrestrial and inland freshwater ecosystems and their services, as well as 15.5 on reducing degradation of natural habitats. The United Nations Decade on Ecosystem Restoration 2021-2030 provides the opportunity to rapidly scale up efforts

A cohesive network approach for modelling fibre and matrix damage in composite laminates

In the current study a high fidelity analysis approach is used to predict the failure process of notched composite structures. Discrete cracking is explicitly modelled by incorporating cohesive interface elements along potential failure paths. These elements form an interconnected network to account for the interaction between interlaminar and intralaminar failure modes. Finite element models of these configurations were created in the commercial analysis software ABAQUS and a user defined material subroutine (UMAT) was used to describe the behaviour of the cohesive elements. The material subroutine ensured that the model remained stable despite significant damage, which is a significant challenge for implicit damage simulations. Two analysis approaches were adopted using either the as-measured or modified (in-situ) ply strengths. Both approaches were capable of closely predicting the mean ultimate strength for a range of hole diameters. However, using the measured ply properties resulted in extensive matrix cracking in the surface ply which caused a deviation from the experimentally measured surface strain. The results demonstrate that high fidelity physically based modelling approaches have the ability to complement or replace certain experimental programs focussed on the design and certification of composite structures

Association between nutritional status and subjective health status in chronically ill children attending special schools

Purpose: In hospitalized children with a chronic disease, malnutrition was associated with a lower subjective health status. In outpatient children with a chronic disease attending special schools, this association has never been studied. The aim of this study was to assess the association between nutritional status and subjective health status in chronically ill children attending special schools. Methods: Overall, 642 children, median age 9.8 years (IQR 7.7–11.5), 60 % male, 72 % Caucasian, were included in this prospective study in nine special schools for chronically ill children in the Netherlands. Overall malnutrition was assessed as: acute malnutrition (<−2 SDS for weight for height (WFH)) and chronic malnutrition (<−2 SDS for height for age). The malnutrition risk was assessed with the nutritional risk-screening tool STRONGkids. Subjective health status was assessed with EQ-5D. Results: Overall, 16 % of the children had overall malnutrition: 3 % acute and 13 % chronic malnutrition. Nurses reported ‘some/severe problems’ on the health status dimensions mobility (15 %), self-care (17 %), usual activities (19 %), pain/discomfort (22 %), and anxiety/depression (22 %) in chronically ill children. Their mean visual analogue scale score (VAS) was 73.0 (SD 11.1). Malnutrition, medication usage, and younger age explained 38 % of the variance of the VAS score. Conclusions: The presence of overall malnutrition in chronically ill children attending special schools was associated with lower subjective health status, especially in younger children and in those with chronic medication usage. Therefore, it is important to develop and use profile-screening tools to identify these children

Identification of major factors influencing ELISpot-based monitoring of cellular responses to antigens from mycobacterium tuberculosis

A number of different interferon-c ELISpot protocols are in use in laboratories studying antigen-specific immune responses. It is therefore unclear how results from different assays compare, and what factors most significantly influence assay outcome. One such difference is that some laboratories use a short in vitro stimulation period of cells before they are transferred to the ELISpot plate; this is commonly done in the case of frozen cells, in order to enhance assay sensitivity. Other differences that may be significant include antibody coating of plates, the use of media with or without serum, the serum source and the number of cells added to the wells. The aim of this paper was to identify which components of the different ELISpot protocols influenced assay sensitivity and inter-laboratory variation. Four laboratories provided protocols for quantifying numbers of interferon-c spot forming cells in human peripheral blood mononuclear cells stimulated with Mycobacterium tuberculosis derived antigens. The differences in the protocols were compared directly. We found that several sources of variation in assay protocols can be eliminated, for example by avoiding serum supplementation and using AIM-V serum free medium. In addition, the number of cells added to ELISpot wells should also be standardised. Importantly, delays in peripheral blood mononuclear cell processing before stimulation had a marked effect on the number of detectable spot forming cells; processing delay thus should be minimised as well as standardised. Finally, a pre-stimulation culture period improved the sensitivity of the assay, however this effect may be both antigen and donor dependent. In conclusion, small differences in ELISpot protocols in routine use can affect the results obtained and care should be given to conditions selected for use in a given study. A pre-stimulation step may improve the sensitivity of the assay, particularly when cells have been previously frozen