Alpha-tocopherol exerts protective function against the mucotoxicity of particulate matter in amphibian and human goblet cells

Exposure to particulate matter (PM) in ambient air is known to increase the risk of cardiovascular disorders and mortality. The cytotoxicity of PM is mainly due to the abnormal increase of reactive oxygen species (ROS), which damage cellular components such as DNA, RNA, and proteins. The correlation between PM exposure and human disorders, including mortality, is based on long-term exposure. In this study we have investigated acute responses of mucus-secreting goblet cells upon exposure to PM derived from a heavy diesel engine. To this end, we employed the mucociliary epithelium of amphibian embryos and human Calu-3 cells to examine PM mucotoxicity. Our data suggest that acute exposure to PM significantly impairs mucus secretion and results in the accumulation of mucus vesicles in the cytoplasm of goblet cells. RNA-seq analysis revealed that acute responses to PM exposure significantly altered gene expression patterns; however, known regulators of mucus production and the secretory pathway were not significantly altered. Interestingly, pretreatment with alpha-tocopherol nearly recovered the hyposecretion of mucus from both amphibian and human goblet cells. We believe this study demonstrates the mucotoxicity of PM and the protective function of alpha-tocopherol on mucotoxicity caused by acute PM exposure from heavy diesel engines

Evolution of the Selenopotential Model and Its Effects on the Propagation Accuracy of Orbits around the Moon

The current work analyzes the effect of applying different selenopotential models to the propagation of a lunar orbiting spacecraft. A brief evolution history of the selenopotential model is first presented; then, four representative selenopotential models are selected for force modeling. Expected propagation errors are presented with respect to three different circular polar orbits around the Moon. As a result, an expected but rather significant number of orbit propagation errors are discovered. Compared to the solutions obtained using the GRAIL1500E model, the overall 3D propagation errors for a 4-day period could reach up to several tens of kilometers (50 km altitude case with the GLGM2 model) and up to several hundreds of meters (50, 100, and 200 km altitude cases even with the GRAIL660B model). For each different orbiter’s altitude, the appropriate ranges of the degree and order of the gravitational harmonic coefficients are also suggested to yield the best propagation performances with respect to the performance obtained with the full harmonic coefficients using the GRAIL1500E model. The results of the current work are expected to serve as practical guidelines for the field of system budget analysis, mission design, mission operations, and the analysis of scientific results

Influence of the Choice of Lunar Gravity Model on Orbit Determination for Lunar Orbiters

We examine the influence of the lunar gravity model on the orbit determination (OD) of a lunar orbiter operating in a 100 km high, lunar polar orbit. Doppler and sequential range measurements by three Deep Space Network antennas and one Korea Deep Space Antenna were used. For measurement simulation and OD analysis, STK11 and ODTK6 were utilized. GLGM2, LP100K, LP150Q, GRAIL420A, and GRAIL660B were used for investigation of lunar gravity model selection effect. OD results were assessed by position and velocity uncertainties with error covariance and an external orbit comparison using simulated true orbit. The effect of the lunar gravity models on the long-term OD, degree and order level, measurement-acquisition condition, and lunar altitude was investigated. For efficiency verification, computational times for the five lunar gravity models were compared. Results showed that significant improvements to OD accuracy are observed by applying a GRAIL-based model; however, applying a full order and degree gravity modeling is not always the best strategy, owing to the computational burden. Consequently, we consider that OD using GRAIL660B with 70 × 70 degree and order is the most efficient strategy for mission preanalysis. This study provides useful guideline for KPLO OD analysis during nominal mission operation

Korea Pathfinder Lunar Orbiter Flight Dynamics Simulation and Rehearsal Results for Its Operational Readiness Checkout

Korea Pathfinder Lunar Orbiter (KPLO), also known as Danuri, was successfully launched on 4 Aug. from Cape Canaveral Space Force Station using a Space-X Falcon-9 rocket. Flight dynamics (FD) operational readiness was one of the critical parts to be checked before the flight. To demonstrate FD software’s readiness and enhance the operator’s contingency response capabilities, KPLO FD specialists planned, organized, and conducted four simulations and two rehearsals before the KPLO launch. For the efficiency and integrity of FD simulation and rehearsal, different sets of blind test data were prepared, including the simulated tracking measurements that incorporated dynamical model errors, maneuver execution errors, and other errors associated with a tracking system. This paper presents the simulation and rehearsal results with lessons learned for the KPLO FD operational readiness checkout. As a result, every functionality of FD operation systems is firmly secured based on the operation procedure with an enhancement of contingency operational response capability. After conducting several simulations and rehearsals, KPLO FD specialists were much more confident in the flight teams’ ability to overcome the challenges in a realistic flight and FD software’s reliability in flying the KPLO. Moreover, the results of this work will provide numerous insights to the FD experts willing to prepare deep space flight operations

Prolonged Activation of ERK Contributes to the Photorejuvenation Effect in Photodynamic Therapy in Human Dermal Fibroblasts

Photodynamic therapy (PDT) is known to be effective in the photorejuvenation of photoaged skin. However, the molecular mechanisms of rejuvenation by PDT remain elusive. In this study, we aimed to understand the molecular events occurring during the photorejuvenation after PDT in dermal fibroblasts in vitro. First, we found that PDT conditions resulted in an increased fibroblast proliferation and motility in vitro. Under this condition, cells had increased intracellular reactive oxygen species (ROS) production. Importantly, PDT induced a prolonged activation of extracellular signal–regulated kinase (ERK) with a corresponding increase in matrix metalloproteinase (MMP)-3 and collagen type Iα messenger RNA and protein. Moreover, inhibition of PDT-induced ERK activation significantly suppressed fibroblast proliferation and expression of MMP-3 and collagen type Iα following PDT. In addition, NAC (an antioxidant) inhibited PDT-induced fibroblast proliferation and ERK activation indicating that prolonged ERK activation and intracellular ROS contribute to the proliferation of fibroblasts and the dermal remodeling process for skin rejuvenation. We also identified increased collagen volume and decreased elastotic materials that are used as markers of photoaging in human skin samples using histochemical studies. Results from this study suggest that intracellular ROS stimulated by PDT in dermal fibroblasts lead to prolonged activation of ERK and, eventually, fibroblast proliferation and activation. Our data thus reveal a molecular mechanism underlying the skin rejuvenation effect of PDT

Development of Brugada Syndrome Following Photodynamic Therapy in a Patient with Cholangiocarcinoma

Brugada syndrome can be unmasked by several conditions including a febrile state, marked leukocytosis, and electrolyte disturbances. Herein, we describe a 62-year-old man with cholangiocarcinoma in the first reported case of Brugada syndrome onset following photodynamic therapy

Atopic Dermatitis-Like Skin Lesions Reduced by Topical Application and Intraperitoneal Injection of Hirsutenone in NC/Nga Mice

Atopic dermatitis (AD) is a common inflammatory skin disease. The increasing prevalence and severity of AD have prompted the developments of safer, more effective drugs. Although topical corticosteroids have been used as first line therapy for AD, their potential side effects limit their clinical applications. To investigate the effect of hirsutenone (HIR), a diarylheptanoid compound, on AD-like skin lesions and other factors related to immune response is the aim of this paper Th2-related cytokines (IL-4, IL-5, IL-13), eosinophil, IgE inflammatory factors (COX-2, iNOS) levels were reduced in blood, lymphocytes, and tissue after HIR treatment. These results suggest that HIR might be an effective treatment for AD