Ago2 Immunoprecipitation Identifies Predicted MicroRNAs in Human Embryonic Stem Cells and Neural Precursors

MicroRNAs are required for maintenance of pluripotency as well as differentiation, but since more microRNAs have been computationally predicted in genome than have been found, there are likely to be undiscovered microRNAs expressed early in stem cell differentiation.SOLiD ultra-deep sequencing identified >10(7) unique small RNAs from human embryonic stem cells (hESC) and neural-restricted precursors that were fit to a model of microRNA biogenesis to computationally predict 818 new microRNA genes. These predicted genomic loci are associated with chromatin patterns of modified histones that are predictive of regulated gene expression. 146 of the predicted microRNAs were enriched in Ago2-containing complexes along with 609 known microRNAs, demonstrating association with a functional RISC complex. This Ago2 IP-selected subset was consistently expressed in four independent hESC lines and exhibited complex patterns of regulation over development similar to previously-known microRNAs, including pluripotency-specific expression in both hESC and iPS cells. More than 30% of the Ago2 IP-enriched predicted microRNAs are new members of existing families since they share seed sequences with known microRNAs.Extending the classic definition of microRNAs, this large number of new microRNA genes, the majority of which are less conserved than their canonical counterparts, likely represent evolutionarily recent regulators of early differentiation. The enrichment in Ago2 containing complexes, the presence of chromatin marks indicative of regulated gene expression, and differential expression over development all support the identification of 146 new microRNAs active during early hESC differentiation

Minstrel of the Appalachians: The Story of Bascom Lamar Lunsford

It is said that Bascom Lamar Lunsford would “cross hell on a rotten rail to get a folk song”—his Southern highlands folk-song compilations now constitute one of the largest collections of its kind in the Library of Congress—but he did much more than acquire songs. He preserved and promoted the Appalachian mountain tradition for generations of people, founding in 1928 the Mountain Dance and Folk Festival in Asheville, North Carolina, an annual event that has shaped America’s festival movement. Loyal Jones pens a lively biography of a man considered to be Appalachian music royalty. He also includes a “Lunsford Sampler” of ballads, songs, hymns, tales, and anecdotes, plus a discography of his recordings. His is a story which richly deserves to be told and what better teller than Loyal Jones. -- Appalachian Heritage A fascinating and important study of one of the individuals responsible for bringing the authentic music of Appalachia to the attention of a wide public. -- Choice While towns and cities were burgeoning musically, trying to promote classical and art-forms over the simple songs of their ancestors, Bascom was desperately throwing his net to catch and hold onto these old time treasures. Loyal Jones has given much insight into the life and times of this amazing man. -- Jean Ritchie This is both a biography and an examination of Lunsford’s career dedicated to the promotion and preservation of Appalachian traditional culture. Jones’s study offers a significant contribution to our ‘new’ culture in the context of folk music. Loyal is an excellent storyteller, and his genial North Carolina manner is perfectly suited to the subject—there is a real affinity between Jones’s authorial voice and Lunsford’s personality. -- Ron Penhttps://uknowledge.uky.edu/upk_appalachian_studies/1002/thumbnail.jp

Mountain Ballads

Phil Conn interviews Loyal Jones, former Director of the Appalachian Center at Berea College, on ballads produced in southern Appalachia. Produced by the Division of Media Services at Morehead State University in 1982

1980-03-04 Our Mountain Heritage: Loyal Jones Part 1&2

Sybil Clark interviews Loyal Jones and showcases his musical talents with old folk songs and ballads for the Our Mountain Heritage series on WMKY, Morehead State University radio, recorded on March 4, 1980, original air date unknown

A Symposium On Sense of Place in Appalachia - Part 7

A symposium on the sense of place in Appalachia held at Morehead State University in October of 1987. The symposium includes presentations from: George Wolford on Sense of Place or Force of Habit (cont.) Clifford Swenson on Sense of Place: A Sacred Symbol Loyal Jones on Music of Appalachia

The relationship between outpatient service use and emergency department visits among people treated for mental and substance use disorders: analysis of population-based administrative data in British Columbia, Canada

Background Research findings on the association between outpatient service use and emergency department (ED) visits for mental and substance use disorders (MSUDs) are mixed and may differ by disorder type. Methods We used population-based linked administrative data in British Columbia, Canada to examine associations between outpatient primary care and psychiatry service use and ED visits among people ages 15 and older, comparing across people treated for three disorder categories: common mental disorders (MDs) (depressive, anxiety, and/or post-traumatic stress disorders), serious MDs (schizophrenia spectrum and/or bipolar disorders), and substance use disorders (SUDs) in 2016/7. We used hurdle models to examine the association between outpatient service use and odds of any ED visit for MSUDs as well count of ED visits for MSUDs, stratified by cohort in 2017/8. Results Having had one or more MSUD-related primary care visit was associated with lower odds of any ED visit among people treated for common MDs and SUDs but not people treated for serious MDs. Continuity of primary care was associated with slightly lower ED use in all cohorts. One or more outpatient psychiatrist visits was associated with lower odds of ED visits among people treated for serious MDs and SUDs, but not among people with common MDs. Conclusion Findings highlight the importance of expanded access to outpatient specialist mental health services, particularly for people with serious MDs and SUDs, and collaborative models that can support primary care providers treating people with MSUDs.Medicine, Faculty ofNon UBCPopulation and Public Health (SPPH), School ofPsychiatry, Department ofReviewedFacultyResearche