The Vehicle, 1969, Vol. 12 no. 1

Vol. 12, No. 1 Table of Contents A New LookNick Dagerpage 3 The RingSara Brinkerhoffpage 5 WaitingSara Brinkerhoffpage 6 Before Cotton FieldsSara Brinkerhoffpage 8 poemAnn Graffpage 9 The Socratic IronyMarcia Trostpage 11 poemNick Dagerpage 17 rainJim Elledgepage 18 LindaMarilyn Viveritopage 19 To You My FatherAnn Flemingpage 20 poemRoger Zulaufpage 24 GeographyJanet Andrewspage 25 Nagging ThoughtJanet Andrewspage 25 Let\u27s RunVerna L. Jonespage 27 Art Credits Kevin SheaCover Mike Dorseypages 4, 23, 28 Steve Williamspages 7, 16, 19, 24, 26 Jim Millerpages 10, 22 Dale Huberpage 13 Nick Dagerpage 3https://thekeep.eiu.edu/vehicle/1021/thumbnail.jp

The Vehicle, Fall 1970

Vol. 13, No. 1 Table of Contents A Thought Written in a Locked RoomJudy Huntpage 1 The Eggshell MoonWilliam Probeckpage 2 PoemBarb Parkerpage 3 4/5, May, 1970J. Michael Sainpage 5 A TreeRichard Stickannpage 6 both or noneMichelle Hallpage 6 The TrainSteve Sestinapage 8 Attempted DiscoveryDonald R. Johnsonpage 16 Island of SmokeVerna L. Jonespage 18 AwakeRobert Bladepage 19 PoemMary Klinkerpage 19 In ChurchMuriel Poolpage 21 PoemBarb Parkerpage 21 PoemMichelle Hallpage 22 Pod\u27nerVerna L. Jonespage 23 Rain and Other ThingsCarol Staniecpage 24 PoemAnn Graffpage 24 Examination of StudentdomMelvin Zaloudekpage 26 Women\u27s LiberationTonya Mortonpage 27 Morning Reflections on the Evening NewsPrudence Herberpage 29 Art and Photography Credits Jim Diaspage 4 Mike Dorseypages 7, 20 David Griffithpages 8, 17, 25 Cover PhotographyMark McKinneyhttps://thekeep.eiu.edu/vehicle/1024/thumbnail.jp

The oral microbiome and breast cancer and nonmalignant breast disease, and its relationship with the fecal microbiome in the Ghana Breast Health Study

The oral microbiome, like the fecal microbiome, may be related to breast cancer risk. Therefore, we investigated whether the oral microbiome was associated with breast cancer and nonmalignant breast disease, and its relationship with the fecal microbiome in a case-control study in Ghana. A total of 881 women were included (369 breast cancers, 93 nonmalignant cases and 419 population-based controls). The V4 region of the 16S rRNA gene was sequenced from oral and fecal samples. Alpha-diversity (observed amplicon sequence variants [ASVs], Shannon index and Faiths Phylogenetic Diversity) and beta-diversity (Bray-Curtis, Jaccard and weighted and unweighted UniFrac) metrics were computed. MiRKAT and logistic regression models were used to investigate the case-control associations. Oral sample alpha-diversity was inversely associated with breast cancer and nonmalignant breast disease with odds ratios (95% CIs) per every 10 observed ASVs of 0.86 (0.83-0.89) and 0.79 (0.73-0.85), respectively, compared to controls. Beta-diversity was also associated with breast cancer and nonmalignant breast disease compared to controls (P ≤ .001). The relative abundances of Porphyromonas and Fusobacterium were lower for breast cancer cases compared to controls. Alpha-diversity and presence/relative abundance of specific genera from the oral and fecal microbiome were strongly correlated among breast cancer cases, but weakly correlated among controls. Particularly, the relative abundance of oral Porphyromonas was strongly, inversely correlated with fecal Bacteroides among breast cancer cases (r = -.37, P ≤ .001). Many oral microbial metrics were strongly associated with breast cancer and nonmalignant breast disease, and strongly correlated with fecal microbiome among breast cancer cases, but not controls

Associations of Circulating Estrogens and Estrogen Metabolites with Fecal and Oral Microbiome in Postmenopausal Women in the Ghana Breast Health Study

ABSTRACT The human fecal and oral microbiome may play a role in the etiology of breast cancer through modulation of endogenous estrogen metabolism. This study aimed to investigate associations of circulating estrogens and estrogen metabolites with the fecal and oral microbiome in postmenopausal African women. A total of 117 women with fecal (N = 110) and oral (N = 114) microbiome data measured by 16S rRNA gene sequencing, and estrogens and estrogen metabolites data measured by liquid chromatography tandem mass spectrometry were included. The outcomes were measures of the microbiome and the independent variables were the estrogens and estrogen metabolites. Estrogens and estrogen metabolites were associated with the fecal microbial Shannon index (global P < 0.01). In particular, higher levels of estrone (β = 0.36, P = 0.03), 2-hydroxyestradiol (β = 0.30, P = 0.02), 4-methoxyestrone (β = 0.51, P = 0.01), and estriol (β = 0.36, P = 0.04) were associated with higher levels of the Shannon index, while 16alpha-hydroxyestrone (β = −0.57, P < 0.01) was inversely associated with the Shannon index as indicated by linear regression. Conjugated 2-methoxyestrone was associated with oral microbial unweighted UniFrac as indicated by MiRKAT (P < 0.01) and PERMANOVA, where conjugated 2-methoxyestrone explained 2.67% of the oral microbial variability, but no other estrogens or estrogen metabolites were associated with any other beta diversity measures. The presence and abundance of multiple fecal and oral genera, such as fecal genera from families Lachnospiraceae and Ruminococcaceae, were associated with several estrogens and estrogen metabolites as indicated by zero-inflated negative binomial regression. Overall, we found several associations of specific estrogens and estrogen metabolites and the fecal and oral microbiome. IMPORTANCE Several epidemiologic studies have found associations of urinary estrogens and estrogen metabolites with the fecal microbiome. However, urinary estrogen concentrations are not strongly correlated with serum estrogens, a known risk factor for breast cancer. To better understand whether the human fecal and oral microbiome were associated with breast cancer risk via the regulation of estrogen metabolism, we conducted this study to investigate the associations of circulating estrogens and estrogen metabolites with the fecal and oral microbiome in postmenopausal African women. We found several associations of parent estrogens and several estrogen metabolites with the microbial communities, and multiple individual associations of estrogens and estrogen metabolites with the presence and abundance of multiple fecal and oral genera, such as fecal genera from families Lachnospiraceae and Ruminococcaceae, which have estrogen metabolizing properties. Future large, longitudinal studies to investigate the dynamic changes of the fecal and oral microbiome and estrogen relationship are needed

ECMO for COVID-19 patients in Europe and Israel

Since March 15th, 2020, 177 centres from Europe and Israel have joined the study, routinely reporting on the ECMO support they provide to COVID-19 patients. The mean annual number of cases treated with ECMO in the participating centres before the pandemic (2019) was 55. The number of COVID-19 patients has increased rapidly each week reaching 1531 treated patients as of September 14th. The greatest number of cases has been reported from France (n = 385), UK (n = 193), Germany (n = 176), Spain (n = 166), and Italy (n = 136) .The mean age of treated patients was 52.6 years (range 16–80), 79% were male. The ECMO configuration used was VV in 91% of cases, VA in 5% and other in 4%. The mean PaO2 before ECMO implantation was 65 mmHg. The mean duration of ECMO support thus far has been 18 days and the mean ICU length of stay of these patients was 33 days. As of the 14th September, overall 841 patients have been weaned from ECMO support, 601 died during ECMO support, 71 died after withdrawal of ECMO, 79 are still receiving ECMO support and for 10 patients status n.a. . Our preliminary data suggest that patients placed on ECMO with severe refractory respiratory or cardiac failure secondary to COVID-19 have a reasonable (55%) chance of survival. Further extensive data analysis is expected to provide invaluable information on the demographics, severity of illness, indications and different ECMO management strategies in these patients

The Vehicle, 1969, Vol. 12 no. 1

Vol. 12, No. 1 Table of Contents A New LookNick Dagerpage 3 The RingSara Brinkerhoffpage 5 WaitingSara Brinkerhoffpage 6 Before Cotton FieldsSara Brinkerhoffpage 8 poemAnn Graffpage 9 The Socratic IronyMarcia Trostpage 11 poemNick Dagerpage 17 rainJim Elledgepage 18 LindaMarilyn Viveritopage 19 To You My FatherAnn Flemingpage 20 poemRoger Zulaufpage 24 GeographyJanet Andrewspage 25 Nagging ThoughtJanet Andrewspage 25 Let\u27s RunVerna L. Jonespage 27 Art Credits Kevin SheaCover Mike Dorseypages 4, 23, 28 Steve Williamspages 7, 16, 19, 24, 26 Jim Millerpages 10, 22 Dale Huberpage 13 Nick Dagerpage 3https://thekeep.eiu.edu/vehicle/1021/thumbnail.jp

The Vehicle, Spring 1971

Vol. 13, No. 2 Table of Contents PoemVerna L. Jonespage 3 PoemSarah Knobelochpage 5 Please, Come AgainLinda Hakepage 6 HaikuMartha McIntyrepage 9 The Eight O\u27Clock TrainCandy Hoempage 10 Late HourMelvin Zaloudekpage 18 SomedayKaren Saxonpage 21 Opus XCecily Hawthornepage 22 Trading CardsWilliam R. Probeckpage 25 MadnessSam Strakapage 26 PoemKristine Kirkhampage 27 Sleepless NightsKen Brewerpage 28 Never SomedayMartha McIntyrepage 31 Art and Photography Credits Judy Novakpage 4 Self PortraitMike Dorseypage 8 My Siege of AltamontMike Dorseypage 16 Marsha Ludlampage 19 Verna L. Jonespage 20 Mark McKinneypage 24, 29, 30 Cover PaintingMike Dorsey Cover PhotographyMark McKinneyhttps://thekeep.eiu.edu/vehicle/1026/thumbnail.jp

Bayesian Evidence for the BoHV-4 sequences studied here.

<p>(A) Mirrored maximum clade credibility trees. (B) Times to most recent common ancestor (tMRCA) posterior densities, medians, and 95% HPD intervals of the studied strains are shown. Branch lengths are proportional to median node heights. The links connecting terminal nodes are colored according to which genotype the corresponding strains belong to (<i>tan</i> Genotype 1; <i>light blue</i> Genotype 2). The link connecting terminals corresponding to strain 08_404 are dashed and gray colored to indicate uncertainty in genotype assignment. <i>Ov</i> strains isolated from ovaries (<i>granulosa cells</i> and <i>oocyte_h3</i>); <i>Gt1</i> Genotype 1; <i>gB</i> glycoprotein B; <i>TK</i> thymidine kinase.</p

Cytopathic effect induced by BoHV-4 strains from bovine ovary batches on MDBK cells.

<p>Cytopathic effect associated with BoHV-4 infections. Light microscope (10X).</p