Age-Related Adaptation of Bone-PDL-Tooth Complex: Rattus-Norvegicus as a Model System

Functional loads on an organ induce tissue adaptations by converting mechanical energy into chemical energy at a cell-level. The transducing capacity of cells alters physico-chemical properties of tissues, developing a positive feedback commonly recognized as the form-function relationship. In this study, organ and tissue adaptations were mapped in the bone-tooth complex by identifying and correlating biomolecular expressions to physico-chemical properties in rats from 1.5 to 15 months. However, future research using hard and soft chow over relevant age groups would decouple the function related effects from aging affects. Progressive curvature in the distal root with increased root resorption was observed using micro X-ray computed tomography. Resorption was correlated to the increased activity of multinucleated osteoclasts on the distal side of the molars until 6 months using tartrate resistant acid phosphatase (TRAP). Interestingly, mononucleated TRAP positive cells within PDL vasculature were observed in older rats. Higher levels of glycosaminoglycans were identified at PDL-bone and PDL-cementum entheses using alcian blue stain. Decreasing biochemical gradients from coronal to apical zones, specifically biomolecules that can induce osteogenic (biglycan) and fibrogenic (fibromodulin, decorin) phenotypes, and PDL-specific negative regulator of mineralization (asporin) were observed using immunohistochemistry. Heterogeneous distribution of Ca and P in alveolar bone, and relatively lower contents at the entheses, were observed using energy dispersive X-ray analysis. No correlation between age and microhardness of alveolar bone (0.7±0.1 to 0.9±0.2 GPa) and cementum (0.6±0.1 to 0.8±0.3 GPa) was observed using a microindenter. However, hardness of cementum and alveolar bone at any given age were significantly different (P<0.05). These observations should be taken into account as baseline parameters, during development (1.5 to 4 months), growth (4 to 10 months), followed by a senescent phase (10 to 15 months), from which deviations due to experimentally induced perturbations can be effectively investigated

Age-related adaptation of bone-PDL-tooth complex: Rattus-Norvegicus as a model system.

Functional loads on an organ induce tissue adaptations by converting mechanical energy into chemical energy at a cell-level. The transducing capacity of cells alters physico-chemical properties of tissues, developing a positive feedback commonly recognized as the form-function relationship. In this study, organ and tissue adaptations were mapped in the bone-tooth complex by identifying and correlating biomolecular expressions to physico-chemical properties in rats from 1.5 to 15 months. However, future research using hard and soft chow over relevant age groups would decouple the function related effects from aging affects. Progressive curvature in the distal root with increased root resorption was observed using micro X-ray computed tomography. Resorption was correlated to the increased activity of multinucleated osteoclasts on the distal side of the molars until 6 months using tartrate resistant acid phosphatase (TRAP). Interestingly, mononucleated TRAP positive cells within PDL vasculature were observed in older rats. Higher levels of glycosaminoglycans were identified at PDL-bone and PDL-cementum entheses using alcian blue stain. Decreasing biochemical gradients from coronal to apical zones, specifically biomolecules that can induce osteogenic (biglycan) and fibrogenic (fibromodulin, decorin) phenotypes, and PDL-specific negative regulator of mineralization (asporin) were observed using immunohistochemistry. Heterogeneous distribution of Ca and P in alveolar bone, and relatively lower contents at the entheses, were observed using energy dispersive X-ray analysis. No correlation between age and microhardness of alveolar bone (0.7 ± 0.1 to 0.9 ± 0.2 GPa) and cementum (0.6 ± 0.1 to 0.8 ± 0.3 GPa) was observed using a microindenter. However, hardness of cementum and alveolar bone at any given age were significantly different (P&lt;0.05). These observations should be taken into account as baseline parameters, during development (1.5 to 4 months), growth (4 to 10 months), followed by a senescent phase (10 to 15 months), from which deviations due to experimentally induced perturbations can be effectively investigated

Histomorphometry as a function of age.

<p>a) Representative light micrograph of first molar distal root at 4X magnification illustrates histomorphometric parameters including i) CEJ-ABC distance, radial widths of: ii) primary cementum, iii) secondary cementum (SC), iv) PDL adjoining primary cementum (PDL-PC), and v) PDL adjoining secondary cementum (PDL-SC). Scale bar = 1 millimeter. b) The table illustrates increasing values of CEJ-ABC distance signifying recession with age. c) Plotted histomorphometric parameters as a function of age comparatively demonstrates increasing cementum and decreasing PDL trends with age.</p

Immunohistochemical staining for identification of SLRPs.

<p>a) Light micrograph at 10X using immunohistochemical methods shows representative localization of SLRPs (biglycan expression in a 6 month old rat) counterstained with hematoxylin. Black arrows indicate localization at mesial PDL-bone enthesis spanning the root length as well as the coronal predominance. In addition localization was also observed in precementum layers (black arrow heads, in Fig. 5a) and at the CDJ (black arrow in inset, Fig. 5a), predentin, and the transseptal fibers (asterisk, Fig. 5a) between any two molars. Scale bars = 500 µm. b) Serially sectioned 60X light micrographs show immunohistochemical localization of SLRPs: biglycan (BGN), decorin (DCN), fibromodulin (FMOD), asporin (ASP), and negative controls (NC). Localization indicates more intense staining in coronal regions relative to apical regions across all age groups. All micrographs indicate regions where PDL meets primary bone (B) and primary cementum (PC) or secondary cementum (SC), with adjacent dentin (D). All Scale bars = 100 µm.</p

Elemental mapping using BSE and EDS.

<p>a) SEM in BSE mode indicated distribution of high and low Z elements of the periodontium. Scale Bar = 0.5 mm. b) Using SEM-BSE greatest heterogeneity is observed in the different layers of bone owing to different chemical compositions of bone during its turnover (i.e. remodeling or modeling). Scale Bar = 100 µm. Representative point shoot EDS demonstrate varying counts of Ca and P (Kα1, Kα2, and Kβ1 lines) within adjacent regions. SE mode indicates at length scales c) the enthesis region of PDL-bone with scale bar = 100 µm, d) the site of a resorption pit, which indicate the digested layers of bone with scale bar = 5 µm, and e) the exposed collagen fibers that help compose a mineralized matrix with scale bar = 4 µm.</p

Morphological changes and anatomical locations within TRAP positive cells.

<p>Morphological changes were observed in histological sections and confirmed in three dimensional space using a MicroXCT (shown in insets comparing occlusal, distal, and mesial surfaces). The distal preference of TRAP positive cells and progressive mesial curving were observed. Anatomical structures include enamel space (ES), dentin (D), periodontal ligament (PDL), alveolar bone (AB), and secondary cementum (SC). TRAP stained micrographs at 4X magnification show first molar distal roots decreasing in osteoclast intensity and frequency with age. Representative coronal and apical TRAP positive areas in the encircled regions are enlarged in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035980#pone-0035980-g003" target="_blank">Figure 3</a>. With age, a transition from CEJ to DGJ (demarcated by white asterisk) and increasing recession (CEJ-ABC distance represented by distance between dashed red lines) were observed. Scale bar = 1 millimeter. CEJ: cementoenamel junction; DGJ: dentin gingival junction; ABC: alveolar bone crest.</p

Localization of sulfated GAGs using alcian blue stain.

<p>a) Light micrographs at 10X of alcian blue with nuclear fast red counterstain indicates more sulfated GAGs on the mesial aspect as indicated by black arrows (shown here in a 1.5 month old rat), as well as around osteocytes in the alveolar bone, precementum (white arrows) and cementum dentin junction (white asterisks). We also observed staining to occur in dentin. Scale bars = 500 µm. b) Corresponding age-related changes at interradicular, coronal-apical, and mesial-distal entheses regions showed decreased alcian blue staining and lost mesial specificity with age (black arrows). Scale bars = 100 µm.</p