Cosmological parameters from the comparison of peculiar velocities with predictions from the 2M++ density field

Peculiar velocity measurements are the only tool available in the low-redshift Universe for mapping the large-scale distribution of matter and can thus be used to constrain cosmology. Using redshifts from the 2M++ redshift compilation, we reconstruct the density of galaxies within 200 Mpc/h, allowing for the first time good sampling of important superclusters such as the Shapley Concentration. We compare the predicted peculiar velocities from 2M++ to Tully-Fisher and SNe peculiar velocities. We find a value of $\beta^* \equiv \Omega_{\rm{m}}^{0.55}/b^* = 0.431 \pm 0.021$, suggesting $\Omega_{\rm{m}}^{0.55}\sigma_{\rm{8,lin}} = 0.401 \pm 0.024$, in good agreement with other probes. The predicted peculiar velocity of the Local Group arising from the 2M++ volume alone is $540 \pm 40$ km/s, towards $l = 268 \pm 4, b= 38 \pm 6$, only $10^\circ$ out of alignment with the Cosmic Microwave Background dipole. To account for velocity contributions arising from sources outside the 2M++ volume, we fit simultaneously for $\beta^*$ and an external bulk flow in our analysis. We find that an external bulk flow is preferred at the 5.1$\sigma$ level, and the best fit has a velocity of $159\pm23$ km/s towards $l=304 \pm 11, b = 6 \pm 13$. Finally, the predicted bulk flow of a 50 Mpc/h Gaussian-weighted volume centred on the Local Group is $230 \pm 30$ km/s, in the direction $l=293\pm 8, b = 14 \pm 10$, in agreement with predictions from $\Lambda$CDM.Comment: 17 pages. MNRAS, in press. Density and peculiar velocity grids available at http://cosmicflows.uwaterloo.ca or http://cosmicflows.iap.f

DAP12 (KARAP) amplifies inflammation and increases mortality from endotoxemia and septic peritonitis

DAP12 (KARAP) is a transmembrane signaling adaptor for a family of innate immunoreceptors that have been shown to activate granulocytes and monocytes/macrophages, amplifying production of inflammatory cytokines. Contrasting with these data, recent studies suggest that DAP12 signaling has an inhibitory role in the macrophage response to microbial products (Hamerman, J.A., N.K. Tchao, C.A. Lowell, and L.L. Lanier. 2005. Nat. Immunol. 6:579–586). To determine the in vivo role for DAP12 signaling in inflammation, we measured the response of wild-type (WT) and DAP12−/− mice to septic shock. We show that DAP12−/− mice have improved survival from both endotoxemia and cecal ligation and puncture–induced septic shock. As compared with WT mice, DAP12−/− mice have decreased plasma cytokine levels and a decreased acute phase response during sepsis, but no defect in the recruitment of cells or bacterial control. In cells isolated after sepsis and stimulated ex vivo, DAP12 signaling augments lipopolysaccharide-mediated cytokine production. These data demonstrate that, during sepsis, DAP12 signaling augments the response to microbial products, amplifying inflammation and contributing to mortality

Breast screening: visual search as an aid for digital mammographic interpretation training

Digital mammography is gradually being introduced across all breast screening centres in the UK during 2010. This provides increased training opportunities using lower resolution, lower cost and more widely available devices, in addition to the clinical digital mammography workstations. This study examined how experienced breast screening personnel performed when they examined sets of difficult DICOM two-view screening cases in three conditions: on GE digital mammography workstations, on a standard LCD monitor (using a DICOM viewer) and an iPhone (running Osirix software). In each condition they either viewed the full images unaided or were permitted to use the post-processing manipulations of pan, zoom and window level/width adjustments. For each case they had to report the feature type, rate their confidence on the presence of abnormality, classify the case and specify case density. Their visual search behaviour was recorded throughout using a head mounted eye tracker. Additionally aspects of their real life screening performance and performance on a national self assessment scheme were examined. Data indicate that screening experience plays a major role in doing well on the self assessment scheme. Task performance was best on the clinical workstation. However, the data also suggest that a DICOM viewer that runs on a PC or laptop with a standard LCD display allows viewing digital images in full resolution support impressive cancer detection performance. The iPhone is not ideal for examining full images due to the amount of scrolling and zooming required. Overall, the results indicate that low cost devices could be used to provide additional tailored training as long as device resolution and HCI aspects are carefully considered

Plant Virus Biodiversity and Ecology

The Plant Virus Biodiversity and Ecology (PVBE) project has been initiated to survey the biodiversity of viruses affecting vascular plants

Evolutionarily distinct Resistance proteins detect a pathogen effector through its association with different host targets

Knowledge of the evolutionary processes which govern pathogen recognition is critical to understanding durable disease resistance. We determined how Phytophthora infestans effector PiAVR2 is recognised by evolutionarily distinct resistance proteins R2 and Rpi-mcq1. We employed yeast two-hybrid, co-immunoprecipitation, virus-induced gene silencing, transient overexpression, and phosphatase activity assays to investigate the contributions of BSL phosphatases to R2- and Rpi-mcq1-mediated hypersensitive response (R2 HR and Rpi-mcq1 HR, respectively). Silencing PiAVR2 target BSL1 compromises R2 HR. Rpi-mcq1 HR is compromised only when BSL2 and BSL3 are silenced. BSL1 overexpression increases R2 HR and compromises Rpi-mcq1. However, overexpression of BSL2 or BSL3 enhances Rpi-mcq1 and compromises R2 HR. Okadaic acid, which inhibits BSL phosphatase activity, suppresses both recognition events. Moreover, expression of a BSL1 phosphatase-dead (PD) mutant suppresses R2 HR, whereas BSL2-PD and BSL3-PD mutants suppress Rpi-mcq1 HR. R2 interacts with BSL1 in the presence of PiAVR2, but not with BSL2 and BSL3, whereas no interactions were detected between Rpi-mcq1 and BSLs. Thus, BSL1 activity and association with R2 determine recognition of PiAVR2 by R2, whereas BSL2 and BSL3 mediate Rpi-mcq1 perception of PiAVR2. R2 and Rpi-mcq1 utilise distinct mechanisms to detect PiAVR2 based on association with different BSLs, highlighting central roles of these effector targets for both disease and disease resistance

Accuracy of GE digital breast tomosynthesis vs supplementary mammographic views for diagnosis of screen-detected soft-tissue breast lesions

Objective: To compare the accuracy of standard supplementary views and GE digital breast tomosynthesis (DBT) for assessment of soft-tissue mammographic abnormalities. Methods: Women recalled for further assessment of soft-tissue abnormalities were recruited and received standard supplementary views (typically spot compression views) and two-view GE DBT. The added value of DBT in the assessment process was determined by analysing data collected prospectively by radiologists working up the cases. Following anonymization of cases, there was also a retrospective multireader review. The readers first read bilateral standard two-view digital mammography (DM) together with the supplementary mammographic views and gave a combined score for suspicion of malignancy on a five-point scale. The same readers then read bilateral standard two-view DM together with two-view DBT. Pathology data were obtained. Differences were assessed using receiver operating characteristic analysis. Results: The study population was 342 lesions in 322 patients. The final diagnosis was malignant in 113 cases (33%) and benign/normal in 229 cases (67%). In the prospective analysis, the performance of two-view DM plus DBT was at least equivalent to the performance of two-view DM and standard mammographic supplementary views—the area under the curve (AUC) was 0.946 and 0.922, respectively, which did not reach statistical significance. Similar results were obtained for the retrospective review—AUC was 0.900 (DBT) and 0.873 (supplementary views), which did not reach statistical significance. Conclusion: The accuracy of GE DBT in the assessment of screen detected soft-tissue abnormalities is equivalent to the use of standard supplementary mammographic views. Advances in knowledge: The vast majority of evidence relating to the use of DBT has been gathered from research using Hologic equipment. This study provides evidence for the use of the commercially available GE DBT system demonstrating that it is at least equivalent to supplementary mammographic views in the assessment of soft-tissue screen-detected abnormalities

Coupled impacts of climate and land use change across a river-lake continuum: Insights from an integrated assessment model of Lake Champlain\u27s Missisquoi Basin, 2000-2040

Global climate change (GCC) is projected to bring higher-intensity precipitation and higher-variability temperature regimes to the Northeastern United States. The interactive effects of GCC with anthropogenic land use and land cover changes (LULCCs) are unknown for watershed level hydrological dynamics and nutrient fluxes to freshwater lakes. Increased nutrient fluxes can promote harmful algal blooms, also exacerbated by warmer water temperatures due to GCC. To address the complex interactions of climate, land and humans, we developed a cascading integrated assessment model to test the impacts of GCC and LULCC on the hydrological regime, water temperature, water quality, bloom duration and severity through 2040 in transnational Lake Champlain\u27s Missisquoi Bay. Temperature and precipitation inputs were statistically downscaled from four global circulation models (GCMs) for three Representative Concentration Pathways. An agent-based model was used to generate four LULCC scenarios. Combined climate and LULCC scenarios drove a distributed hydrological model to estimate river discharge and nutrient input to the lake. Lake nutrient dynamics were simulated with a 3D hydrodynamic-biogeochemical model. We find accelerated GCC could drastically limit land management options to maintain water quality, but the nature and severity of this impact varies dramatically by GCM and GCC scenario

Nanoimprinted distributed feedback lasers of solution processed hybrid perovskites

Hybrid perovskite materials have considerable potential for light emitting devices such as LEDs and lasers. We combine solution processed CH3NH3PbI3 perovskite with UV nanoimprinted polymer gratings to fabricate distributed feedback (DFB) lasers. The lead acetate deposition route is shown to be an effective method for fabricating low-loss waveguides (loss coefficient ~6 cm-1) and highly compatible with the polymer grating substrates. The nanoimprinted perovskite exhibited single-mode band-edge lasing, confirmed by angle-dependent transmission measurements. Depending on the excitation pulse duration the lasing threshold shows a value of 110 μJ/cm2 under nanosecond pumping and 4 μJ/cm2 under femtosecond pumping. We demonstrate further that this laser has excellent stability with a lifetime of 10*8 pulses