Supporting Cells Eliminate Dying Sensory Hair Cells to Maintain Epithelial Integrity in the Avian Inner Ear

Epithelial homeostasis is essential for sensory transduction in the auditory and vestibular organs of the inner ear, but how it is maintained during trauma is poorly understood. To examine potential repair mechanisms, we expressed beta-actin-enhanced green fluorescent protein (EGFP) in the chick inner ear and used live-cell imaging to study how sensory epithelia responded during aminoglycoside-induced hair cell trauma. We found that glial-like supporting cells used two independent mechanisms to rapidly eliminate dying hair cells. Supporting cells assembled an actin cable at the luminal surface that extended around the pericuticular junction and constricted to excise the stereocilia bundle and cuticular plate from the hair cell soma. Hair bundle excision could occur within 3 min of actin-cable formation. After bundle excision, typically with a delay of up to 2-3 h, supporting cells engulfed and phagocytosed the remaining bundle-less hair cell. Dual-channel recordings with beta-actin-EGFP and vital dyes revealed phagocytosis was concurrent with loss of hair cell integrity. We conclude that supporting cells repaired the epithelial barrier before hair cell plasmalemmal integrity was lost and that supporting cell activity was closely linked to hair cell death. Treatment with the Rho-kinase inhibitor Y-27632 did not prevent bundle excision but prolonged phagocytic engulfment and resulted in hair cell corpses accumulating within the epithelium. Our data show that supporting cells not only maintain epithelial integrity during trauma but suggest they may also be an integral part of the hair cell death process itself

Fast and Fourier: Extreme Mass Ratio Inspiral Waveforms in the Frequency Domain

Extreme Mass Ratio Inspirals (EMRIs) are one of the key sources for future space-based gravitational wave interferometers. Measurements of EMRI gravitational waves are expected to determine the characteristics of their sources with sub-percent precision. However, their waveform generation is challenging due to the long duration of the signal and the high harmonic content. Here, we present the first ready-to-use Schwarzschild eccentric EMRI waveform implementation in the frequency domain for use with either graphics processing units (GPUs) or central processing units (CPUs). We present the overall waveform implementation and test the accuracy and performance of the frequency domain waveforms against the time domain implementation. On GPUs, the frequency domain waveform takes in median $0.044$ seconds to generate and is twice as fast to compute as its time domain counterpart when considering massive black hole masses $\geq 2 \times 10^6 \,{\rm M_\odot}$ and initial eccentricities $e_0 > 0.2$. On CPUs, the median waveform evaluation time is $5$ seconds, and it is five times faster in the frequency domain than in the time domain. Using a sparser frequency array can further speed up the waveform generation, reaching up to $0.3$ seconds. This enables us to perform, for the first time, EMRI parameter inference with fully relativistic waveforms on CPUs. Future EMRI models which encompass wider source characteristics (particularly black hole spin and generic orbit geometries) will require significantly more harmonics. Frequency-domain models will be essential analysis tools for these astrophysically realistic and important signals.Comment: 23 pages, 6 figure

MAGNETO - ELECTRIC LOGIC DEVICES USING SEMICONDUCTOR CHANNEL WITH LARGE SPIN - ORBIT COUPLING

Antiferromagnetic magneto - electric spin - orbit read ( AF SOR ) logic devices are presented . The devices include a voltage - controlled magnetoelectric ( ME ) layer that switches polarization in response to an electric field from the applied voltage and a narrow channel conductor of a spin - orbit coupling ( SOC ) material on the ME layer . One or more sources and one or more drains , each optionally formed of ferromagnetic material , are provided on the SOC material

Structural basis for tunable control of actin dynamics by myosin-15 in mechanosensory stereocilia

The motor protein myosin-15 is necessary for the development and maintenance of mechanosensory stereocilia, and mutations in myosin-15 cause hereditary deafness. In addition to transporting actin regulatory machinery to stereocilia tips, myosin-15 directly nucleates actin filament (“F-actin”) assembly, which is disrupted by a progressive hearing loss mutation (p.D1647G, “jordan”). Here, we present cryo–electron microscopy structures of myosin-15 bound to F-actin, providing a framework for interpreting the impacts of deafness mutations on motor activity and actin nucleation. Rigor myosin-15 evokes conformational changes in F-actin yet maintains flexibility in actin’s D-loop, which mediates inter-subunit contacts, while the jordan mutant locks the D-loop in a single conformation. Adenosine diphosphate–bound myosin-15 also locks the D-loop, which correspondingly blunts actin-polymerization stimulation. We propose myosin-15 enhances polymerization by bridging actin protomers, regulating nucleation efficiency by modulating actin’s structural plasticity in a myosin nucleotide state–dependent manner. This tunable regulation of actin polymerization could be harnessed to precisely control stereocilium height

Numerical Study of Magnetoaerodynamic Flow Around a Hemisphere

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/83560/1/AIAA-49278-455.pd

Gamma-Ray Bursts and Magnetars as Possible Sources of Ultra High Energy Cosmic Rays: Correlation of Cosmic Ray Event Positions with IRAS Galaxies

We use the two-dimensional Kolmogorov-Smirnov (KS) test to study the correlation between the 60 cosmic ray events above 4x10^19 eV from the AGASA experiment and the positions of infrared luminous galaxies from the IRAS PSCz catalog. These galaxies are expected to be hosts to gamma ray bursts (GRB) and magnetars, both of which are associated with core collapse supernovae and have been proposed as possible acceleration sites for ultra high energy cosmic rays. We find consistency between the models and the AGASA events to have been drawn from the same underlying distribution of positions on the sky with KS probabilities ~50%. Application of the same test to the 11 highest AGASA events above 10^20 eV, however, yields a KS probability of < 0.5%, rejecting the models at >99.5% significance level. Taken at face value, these highest energy results suggest that the existing cosmic ray events above 10^20 eV do not owe their origin to long burst GRBs, rapidly rotating magnetars, or any other events associated with core collapse supernovae. The larger data set expected from the AUGER experiment will test whether this conclusion is real or is a statistical fluke that we estimate to be at the 2 sigma level.Comment: 15 pages, 4 figures. Final Version to be published in Phys. Rev.

Modular Robotic Vehicle

A modular robotic vehicle includes a chassis, driver input devices, an energy storage system (ESS), a power electronics module (PEM), modular electronic assemblies (eModules) connected to the ESS via the PEM, one or more master controllers, and various embedded controllers. Each eModule includes a drive wheel containing a propulsion-braking module, and a housing containing propulsion and braking control assemblies with respective embedded propulsion and brake controllers, and a mounting bracket covering a steering control assembly with embedded steering controllers. The master controller, which is in communication with each eModule and with the driver input devices, communicates with and independently controls each eModule, by-wire, via the embedded controllers to establish a desired operating mode. Modes may include a two-wheel, four-wheel, diamond, and omni-directional steering modes as well as a park mode. A bumper may enable docking with another vehicle, with shared control over the eModules of the vehicles

The Lifetime of FRIIs in Groups and Clusters: Implications for Radio-Mode Feedback

We determine the maximum lifetime t_max of 52 FRII radio sources found in 26 central group galaxies from cross correlation of the Berlind SDSS group catalog with the VLA FIRST survey. Mock catalogs of FRII sources were produced to match the selection criteria of FIRST and the redshift distribution of our parent sample, while an analytical model was used to calculate source sizes and luminosities. The maximum lifetime of FRII sources was then determined via a comparison of the observed and model projected length distributions. We estimate the average FRII lifetime is 1.5x10^7 years and the duty cycle is ~8x10^8 years. Degeneracies between t_max and the model parameters: jet power distribution, axial ratio, energy injection index, and ambient density introduce at most a factor of two uncertainty in our lifetime estimate. In addition, we calculate the radio active galactic nuclei (AGN) fraction in central group galaxies as a function of several group and host galaxy properties. The lifetime of radio sources recorded here is consistent with the quasar lifetime, even though these FRIIs have substantially sub-Eddington accretion. These results suggest a fiducial time frame for energy injection from AGN in feedback models. If the morphology of a given extended radio source is set by large-scale environment, while the lifetime is determined by the details of the accretion physics, this FRII lifetime is relevant for all extended radio sources.Comment: 18 pages, 7 figures. Accepted for publication in ApJ. High resolution paper available at http://www.astronomy.ohio-state.edu/~bird/BMK07.pd