14 research outputs found
Perfusion bioreactors for bone tissue engineering: A combined experimental and computational approach
The influence of curvature on three-dimensional mineralized matrix formation under static and perfused conditions: an in vitro
Scaffold Pore Geometry Guides Gene Regulation and Bone-like Tissue Formation in Dynamic Cultures
Cells sense and respond to scaffold pore geometry and mechanical stimuli. Many fabrication methods used in bone tissue engineering render structures with poorly controlled pore geometries. Given that cell-scaffold interactions are complex, drawing a conclusion on how cells sense and respond to uncontrolled scaffold features under mechanical loading is difficult. In this study, monodisperse templated scaffolds (MTSC) were fabricated and used as well-defined porous scaffolds to study the effect of dynamic culture conditions on bone-like tissue formation. Human bone marrow-derived stromal cells were cultured on MTSC or conventional salt-leached scaffolds (SLSC) for up to 7 weeks, either under static or dynamic conditions (wall shear stress [WSS] using spinner flask bioreactors). The influence of controlled spherical pore geometry of MTSC subjected to static or dynamic conditions on osteoblast cell differentiation, bone-like tissue formation, structure, and distribution was investigated. WSS generated within the two idealized geometrical scaffold features was assessed. Distinct response to fluid flow in osteoblast cell differentiation were shown to be dependent on scaffold pore geometry. As revealed by collagen staining and microcomputed tomography images, dynamic conditions promoted a more regular extracellular matrix (ECM) formation and mineral distribution in both scaffold types compared with static conditions. The results showed that regulation of bone-related genes and the amount and the structure of mineralized ECM were dependent on scaffold pore geometry and the mechanical cues provided by the two different culture conditions. Under dynamic conditions, SLSC favored osteoblast cell differentiation and ECM formation, whereas MTSC enhanced ECM mineralization. The spherical pore shape in MTSC supported a more trabecular bone-like structure under dynamic conditions compared with MTSC statically cultured or to SLSC under either static or dynamic conditions. These results suggest that cell activity and bone-like tissue formation is driven not only by the pore geometry but also by the mechanical environment. This should be taken into account in the future design of complex scaffolds, which should favor cell differentiation while guiding the formation, structure, and distribution of the engineered bone tissue. This could help to mimic the anatomical complexity of the bone tissue structure and to adapt to each bone defect needs. Impact statement Aging of the human population leads to an increasing need for medical implants with high success rate. We provide evidence that cell activity and the amount and structure of bone-like tissue formation is dependent on the scaffold pore geometry and on the mechanical environment. Fabrication of complex scaffolds comprising concave and planar pore geometries might represent a promising direction toward the tunability and mimicry the structural complexity of the bone tissue. Moreover, the use of fabrication methods that allow a systematic fabrication of reproducible and geometrically controlled structures would simplify scaffold design optimization
Scaffold Pore Geometry Guides Gene Regulation and Bone-like Tissue Formation in Dynamic Cultures
Cells sense and respond to scaffold pore geometry and mechanical stimuli. Many fabrication methods used in bone tissue engineering render structures with poorly controlled pore geometries. Given that cell–scaffold interactions are complex, drawing a conclusion on how cells sense and respond to uncontrolled scaffold features under mechanical loading is difficult. In this study, monodisperse templated scaffolds (MTSC) were fabricated and used as well-defined porous scaffolds to study the effect of dynamic culture conditions on bone-like tissue formation. Human bone marrow-derived stromal cells were cultured on MTSC or conventional salt-leached scaffolds (SLSC) for up to 7 weeks, either under static or dynamic conditions (wall shear stress [WSS] using spinner flask bioreactors). The influence of controlled spherical pore geometry of MTSC subjected to static or dynamic conditions on osteoblast cell differentiation, bone-like tissue formation, structure, and distribution was investigated. WSS generated within the two idealized geometrical scaffold features was assessed. Distinct response to fluid flow in osteoblast cell differentiation were shown to be dependent on scaffold pore geometry. As revealed by collagen staining and microcomputed tomography images, dynamic conditions promoted a more regular extracellular matrix (ECM) formation and mineral distribution in both scaffold types compared with static conditions. The results showed that regulation of bone-related genes and the amount and the structure of mineralized ECM were dependent on scaffold pore geometry and the mechanical cues provided by the two different culture conditions. Under dynamic conditions, SLSC favored osteoblast cell differentiation and ECM formation, whereas MTSC enhanced ECM mineralization. The spherical pore shape in MTSC supported a more trabecular bone-like structure under dynamic conditions compared with MTSC statically cultured or to SLSC under either static or dynamic conditions. These results suggest that cell activity and bone-like tissue formation is driven not only by the pore geometry but also by the mechanical environment. This should be taken into account in the future design of complex scaffolds, which should favor cell differentiation while guiding the formation, structure, and distribution of the engineered bone tissue. This could help to mimic the anatomical complexity of the bone tissue structure and to adapt to each bone defect needs
Pressureless Mechanical Induction of Stem Cell Differentiation Is Dose and Frequency Dependent
<div><p>Movement is a key characteristic of higher organisms. During mammalian embryogenesis fetal movements have been found critical to normal tissue development. On the single cell level, however, our current understanding of stem cell differentiation concentrates on inducing factors through cytokine mediated biochemical signaling. In this study, human mesenchymal stem cells and chondrogenesis were investigated as representative examples. We show that pressureless, soft mechanical stimulation precipitated by the cyclic deformation of soft, magnetic hydrogel scaffolds with an external magnetic field, can induce chondrogenesis in mesenchymal stem cells without any additional chondrogenesis transcription factors (TGF-β1 and dexamethasone). A systematic study on the role of movement frequency revealed a classical dose-response relationship for human mesenchymal stem cells differentiation towards cartilage using mere mechanical stimulation. This effect could even be synergistically amplified when exogenous chondrogenic factors and movement were combined.</p> </div
Frequency dependent hMSC differentiation.
<p>Mechanical stimulation frequency influences the differentiation and formation of tissue-typical extracellular matrix (amount of GAG formed) in both control and chondrogenic medium. Cells were pressure-free stretched on soft scaffolds for 2 seconds (stimulation period). Non-moved scaffolds (left) served as additional controls. The amount of GAG deposition indicated differentiation on all mechanical stimulated scaffolds particularly at high frequency. This behavior shows that mechanical soft movement follows a dose-effect type response similar to a classical response of specific cells to a given biochemical factor.</p
Chondrogenesis on magnetic hydrogels with and without mechanical stimulation.
<p>Aggrecan (antibody labeling, red), SOX9 (antibody labeling, green) and Collagen II expression (green) immunohistochemistry of hMSC over a period of 5 weeks. Cell cultures were either not stimulated or underwent repeated mechanical stimulation (left). The role of movement was investigated both in control medium (top rows) and in standard chondrogenic medium (bottom rows). Samples were counterstained with DAPI to make cell nuclei visible. Mechanical stimulation resulted in clear up-regulation of all chondrogenic markers if compared to the non-stimulated control cultures.</p
Mechanical stimulation induced chondrogenesis.
<p>a) Cell numbers (DNA amount per scaffold) confirmed good cell expansion and growth. Below is the glycosaminoglycan (GAG) deposition per scaffold over a period of 5 weeks. Control medium (white bars) and chondrogenic medium (grey bars) were applied on cells seeded into either tissue culture plate (no scaffold), hydrogel scaffold (no nanomagnets, i.e. no movement is possible) or magnetic hydrogel. Mechanical stimulation (arrow) triggered higher GAG deposition. b) Comparable DNA amount indicated good cell growth for cells seeded into magnetic hydrogels with both medium types and no negative effects from mechanical stimulation. GAG deposition using diluted chondrogenic (grey) versus control medium (white bars). Mechanically stimulated hMSC in control medium showed comparable GAG deposition as in standard chondrogenic medium under magnetic actuation (indicated by ↕). * p < 0.01 cells cultured with control medium under mechanical stimulation versus non stimulated and mechanically stimulated hydrogel using both cell culture media.</p