Integrative and Conjugative Elements (ICEs): What They Do and How They Work

Horizontal gene transfer plays a major role in microbial evolution, allowing microbes to acquire new genes and phenotypes. Integrative and conjugative elements (ICEs, a.k.a. conjugative transposons) are modular mobile genetic elements integrated into a host genome and are passively propagated during chromosomal replication and cell division. Induction of ICE gene expression leads to excision, production of the conserved conjugation machinery (a type IV secretion system), and the potential to transfer DNA to appropriate recipients. ICEs typically contain cargo genes that are not usually related to the ICE life cycle and that confer phenotypes to host cells. We summarize the life cycle and discovery of ICEs, some of the regulatory mechanisms, and how the types of cargo have influenced our view of ICEs. We discuss how ICEs can acquire new cargo genes and describe challenges to the field and various perspectives on ICE biology

Ultrafast carrier dynamics in thin-films of the topological insulator Bi2Se3

Transient reflectivity measurements of thin films, ranging from 6 to 40 nm in thickness, of the topological insulator Bi2Se3 revealed a strong dependence of the carrier relaxation time on the film thickness. For thicker films the relaxation dynamics are similar to those of bulk Bi2Se3, where the contribution of the bulk insulating phase dominates over that of the surface metallic phase. The carrier relaxation time shortens with decreasing film thickness, reaching values comparable to those of noble metals. This effect may result from the hybridization of Dirac cone states at the opposite surfaces for the thinnest films

Identification of a Single Strand Origin of Replication in the Integrative and Conjugative Element ICEBs1 of Bacillus subtilis

We identified a functional single strand origin of replication (sso) in the integrative and conjugative element ICEBs1 of Bacillus subtilis. Integrative and conjugative elements (ICEs, also known as conjugative transposons) are DNA elements typically found integrated into a bacterial chromosome where they are transmitted to daughter cells by chromosomal replication and cell division. Under certain conditions, ICEs become activated and excise from the host chromosome and can transfer to neighboring cells via the element-encoded conjugation machinery. Activated ICEBs1 undergoes autonomous rolling circle replication that is needed for the maintenance of the excised element in growing and dividing cells. Rolling circle replication, used by many plasmids and phages, generates single-stranded DNA (ssDNA). In many cases, the presence of an sso enhances the conversion of the ssDNA to double-stranded DNA (dsDNA) by enabling priming of synthesis of the second DNA strand. We initially identified sso1 in ICEBs1 based on sequence similarity to the sso of an RCR plasmid. Several functional assays confirmed Sso activity. Genetic analyses indicated that ICEBs1 uses sso1 and at least one other region for second strand DNA synthesis. We found that Sso activity was important for two key aspects of the ICEBs1 lifecycle: 1) maintenance of the plasmid form of ICEBs1 in cells after excision from the chromosome, and 2) stable acquisition of ICEBs1 following transfer to a new host. We identified sequences similar to known plasmid sso's in several other ICEs. Together, our results indicate that many other ICEs contain at least one single strand origin of replication, that these ICEs likely undergo autonomous replication, and that replication contributes to the stability and spread of these elements.National Institute of General Medical Sciences (U.S.) (Award R01GM050895)National Institute of General Medical Sciences (U.S.) (Pre-Doctoral Training Grant T32GM007287

Competitive advantage and fuel efficiency in aviation

This paper builds upon a resource based view of competitive advantage under a dynamic capabilities construct. Fuel efficiency measurement in the aviation industry can be incorporated into dynamic capabilities such as strategic decision making and alliancing. These dynamic capabilities can drive operational cost reductions, which in-turn can enhance profitability and establish a competitive advantage. To further this advantage, fuel efficiency can be embedded inside an organizational culture. A fuel efficiency focused organizational culture can be a valuable, rare, inimitable and non- substitutable resource. This paper proposes a model to merge the dynamic capabilities of strategic decision making and alliancing with organizational culture under fuel efficiency. Under this model, a fuel efficiency index is introduced to drive behavior and provide accountability. Effective use of the index has profit potential

The Myth of Strategic and Tactical Airlift

In the 21st century, our ability to quickly and decisively deliver combat forces and equipment is of the utmost importance in achieving our national security objectives. The swiftness and flexibility of the US Air Force’s mobility airlift fleet is the key to executing a rapid global mobility strategy. The operational effectiveness and efficiency of military air transportation relies on the expertise and intuition of Air Mobility Command’s (AMC) mobility planners. Working in coordination with the United States Transportation Command (USTRANSCOM) and geographic combatant commands (GCC), AMC is responsible for the tasking and tracking of almost 900 daily mobility sorties worldwide. Using a hub-and-spoke model, mobility planners conceptualize airlift requirements and routes as either tactical or strategic in nature. Airlift assets are also considered this way. Tactical aircraft (usually C-130 variants) are smaller and are used primarily for intratheater airlift within a defined area of responsibility (AOR). Strategic aircraft (C-5B/M, C-17A) have larger payload capacities and extended ranges, making them useful for intertheater transportation between two different AORs or GCCs

Asymmetric three-beam binary optic grating

Binary and diffractive optical components are finding many applications in optical systems and integrated optical devices. A recent application required the development of a two-dimensional diffraction grating to perform an asymmetric, three-beam fanout. In this paper, techniques are presented for the design of arbitrary fanout grating devices. Modeling and optimization processes are demonstrated for the three-beam grating. The counter-intuitive results of the initial design are discussed, and experimental data verifying performance are presented

Optical Properties of the Kara Sea

This study was motivated by the need to understand dispersion processes which affect the redistribution of nuclear wastes in the Arctic from dump sites in the Kara Sea and in the rivers which flow into the Kara Sea. We focus on vertical profiles of light beam transmission and fluorometry made over the delta region fronting the Ob and Yenisey Rivers and over the East Novaya Zemlya Trough (ENZT). The delta region fronting the Ob River Estuary contains a large repository of particles in a dense bottom nepheloid layer with a maximum centered similar to 100 km in front of the estuary entrance and covering an area of roughly 200 km diameter. This suspended particle mass repository appears to contain both sediments and detritus and lends credence to the Lisitsyn [1995] concept of the marginal filter zone. In the deep water of the ENZT we found a strong increase of beam attenuation with depth, indicating a relatively large increase of particle mass concentration from similar to 50 m to the bottom (depths in excess of 300 m). The strongest concentration was adjacent to the southeast coast of Novaya Zemlya. We suggest that a type of hyperpycnical flow occurs from accumulation of sediments in the bottom waters of Novaya Zemlya fjords which then cascades down the steep slopes adjacent to the island, producing the particle mass distribution as observed by the transmissometer. The accumulation of these repositories of high particle mass concentrations in suspension would suggest that the residence time is high but that storm-driven events could act to disperse the material