Forager bees (Apis mellifera) highly express immune and detoxification genes in tissues associated with nectar processing.

Pollinators, including honey bees, routinely encounter potentially harmful microorganisms and phytochemicals during foraging. However, the mechanisms by which honey bees manage these potential threats are poorly understood. In this study, we examine the expression of antimicrobial, immune and detoxification genes in Apis mellifera and compare between forager and nurse bees using tissue-specific RNA-seq and qPCR. Our analysis revealed extensive tissue-specific expression of antimicrobial, immune signaling, and detoxification genes. Variation in gene expression between worker stages was pronounced in the mandibular and hypopharyngeal gland (HPG), where foragers were enriched in transcripts that encode antimicrobial peptides (AMPs) and immune response. Additionally, forager HPGs and mandibular glands were enriched in transcripts encoding detoxification enzymes, including some associated with xenobiotic metabolism. Using qPCR on an independent dataset, we verified differential expression of three AMP and three P450 genes between foragers and nurses. High expression of AMP genes in nectar-processing tissues suggests that these peptides may contribute to antimicrobial properties of honey or to honey bee defense against environmentally-acquired microorganisms. Together, these results suggest that worker role and tissue-specific expression of AMPs, and immune and detoxification enzymes may contribute to defense against microorganisms and xenobiotic compounds acquired while foraging

Analyzing The Effectiveness Of The Gurney Method For Small Scale Fragmentation Propulsion Using Exploding Bridgewire Detonators

Explosives are common in military, mining, and construction applications where the explosive properties are understood, but mechanics of how the explosive\u27s energy fragments and throws materials are less known. Considering the type of confining material around an explosive, creates variability in fragmentation behavior due to the individual material characteristics. The most common method for assessing fragmentation behavior is the Gurney method, which eliminates any consideration of fragmenting material properties. The Gurney method assumes that, on a large scale, the inconsistencies in material are irrelevant and only the mass of the confiner need be considered. However, it is known in many fields that energy is consumed in the breaking of a material. In this paper, the detonation and resultant fragmentation propulsion of Exploding Bridgewire (EBW) detonators with the same explosive material, but different casing characteristics, is observed. The Gurney method was used to predict fragmentation velocities of the casing following detonation, which were compared to the behavior observed through high-speed video of the actual event. The EBWs were selected to provide variability in casing material, casing thickness, charge length, and charge diameter. It was found that when the amount of explosive is small, the material properties of the casing play a significant role, with 70 % of the total explosive mass lost in fragmenting PMMA EBWs and 30±3 % lost in Aluminum EBWs. There is significant energy loss to breaking the casing material that cannot be ignored on the small scale and could impact large explosives with high casing to explosive ratios

Speaking Freely and Freedom of Speech: Feminists Navigating the "New" Right

An introduction to the special section “Speaking Freely and Freedom of Speech: Feminists Navigating the ‘New’ Right.

Water-Cased Kicker Charges for Use in Explosive Demolition

Demolition Projects Involving Explosives Often Incorporate Cutting Charges to Sever Columns in Conjunction with Kicker Charges that Move the Columns Out of Alignment. Traditional Kicker Charges Use Dynamite Secured to the Column above a Linear-Shaped Cutting Charge. This Study Investigates the Use of Water-Cased Kicker Charges for Use in Explosive Demolition. the Goal is to Reduce the Fragmentation of Steel Members and the Quantity of Explosive Needed Due to the Increased Density, Incompressibility, and Impedance Mismatch Water Provides. Simulations and Experimental Tests Were Utilized to Determine What Type of Charges Provide the Optimal Column Movement and Water Placement. Water Charges and Traditional Charges Were Placed on Hanging Steel Columns that Swung Freely from a Top Pivot and Analyzed for the Fragmentation and Velocity of the Column. Tests Were Recorded with High-Speed Video to Calculate Velocity and Impulse. Simulations Showed the Same Results as Experimental Tests, with Water-Cased Charges Moving the Column Faster and with More Impulse Than Traditional Charges. Experimental Testing Showed that Water-Cased Charges Moved the Column 53% Faster Than Traditional in Contact Charges While Simulations Showed that Water-Cased Charges Moved the Column 43% Faster Than Traditional in Contact Charges. Simulations Showed the Water Tamped Behind the Charge Increased Beam Velocity 32% While Water in Front of the Charge Reduced Pressure 38% through Dispersion

Effect Of Shock Tunnel Geometry On Shockwave And Vortex Ring Formation, Propagation, And Head On Collision

Vortex ring research primarily focuses on the formation from circular openings. Consequently, the role of tunnel geometry is less understood, despite there being numerous research studies using noncircular shock tunnels. This experimental study investigated shockwaves and vortex rings from different geometry shock tunnels from formation at the tunnel opening to head on collision with another similarly formed vortex ring using schlieren imaging and statistical analysis. The velocity of the incident shockwave was found to be consistent across all four shock tunnel geometries, which include circle, hexagon, square, and triangle of the same cross-sectional area. The velocity was 1.2 ± 0.007 Mach and was independent of the tunnel geometry. However, the velocities of the resulting vortex rings differed between the shapes, with statistical analysis indicating significant differences between the triangle and hexagon vortex velocities compared to the circle. Vortex rings from the square and circle shock tunnels were found to have statistically similar velocities. All vortex rings slowed as they traveled due to corner inversion and air drag. All shock tunnels with corners produce a wobble in the vortex rings. Vortex rings interact with opposing incident shockwaves prior to colliding with each other. Vortex velocity before and after shock-vortex interaction was measured and evaluated, showing statistically similar results. Shock-vortex interaction slows the shockwave upon interaction, while the shock-shock interaction resulted in no change in shock velocity. Although the vortex rings travel at different velocities, all head-on vortex ring collisions produce a perpendicular shockwave that travels at 1.04 ± 0.005 Mach

A rational approach to selecting and ranking some pharmaceuticals of concern for the aquatic environment and their relative importance compared with other chemicals

Aquatic organisms can be exposed to thousands of chemicals discharged by the human population. Many of these chemicals are considered disruptive to aquatic wildlife; the literature on the impacts of these chemicals grows daily. However, since time and resources are not infinite, we must focus on the chemicals which represent the greatest threat. One group of chemicals of increasing concern is the pharmaceuticals, where the struggle is to identify which of them represent the greatest threat. In the present study, we compiled a list of 12 pharmaceuticals based on scoring the prevalence of different compounds from previous prioritization reviews. These included rankings based on prescription data, environmental concentrations, PEC/PNEC ratios, PBT, and fish plasma model approaches. The most frequently cited were diclofenac, paracetamol, ibuprofen, carbamazepine, naproxen, atenolol, ethinylestradiol, aspirin, fluoxetine, propranolol, metoprolol and sulfamethoxazole. For each pharmaceutical, literature on effect concentrations was compiled and compared with river concentrations in the UK. The pharmaceuticals were ranked on the degree of difference between the median effect and median river concentrations. EE2 was ranked as the highest concern, followed by fluoxetine, propranolol and paracetamol. The relative risk of these pharmaceuticals was compared with those of metals and some persistent organic pollutants. Pharmaceuticals appear to be less of a threat to aquatic organisms than some metals (Cu, Al, Zn) and triclosan using this ranking approac

ADOPTION OF BEST MANAGEMENT PRACTICES IN STOCKER CATTLE PRODUCTION

This study identifies current production and management practices of Oklahoma stocker cattle producers and analyzes factors affecting the adoption of best management practices (BMPs) using chi-square analysis. Results reveal that factors influencing the adoption of BMPs are operation size, dependency upon income from the operation, and specialization in stocker production.cattle, stockers, management, production, Livestock Production/Industries,

Viral metagenomics, protein structure, and reverse genetics: Key strategies for investigating coronaviruses

Viral metagenomics, modeling of protein structure, and manipulation of viral genetics are key approaches that have laid the foundations of our understanding of coronavirus biology. In this review, we discuss the major advances each method has provided and discuss how future studies should leverage these strategies synergistically to answer novel questions

Total hip replacement for the treatment of end stage arthritis of the hip : a systematic review and meta-analysis

Background: Evolvements in the design, fixation methods, size, and bearing surface of implants for total hip replacement (THR) have led to a variety of options for healthcare professionals to consider. The need to determine the most optimal combinations of THR implant is warranted. This systematic review evaluated the clinical effectiveness of different types of THR used for the treatment of end stage arthritis of the hip. Methods: A comprehensive literature search was undertaken in major health databases. Randomised controlled trials (RCTs) and systematic reviews published from 2008 onwards comparing different types of primary THR in patients with end stage arthritis of the hip were included. Results: Fourteen RCTs and five systematic reviews were included. Patients experienced significant post-THR improvements in Harris Hip scores, but this did not differ between impact types. There was a reduced risk of implant dislocation after receiving a larger femoral head size (36 mm vs. 28 mm; RR = 0.17, 95% CI: 0.04, 0.78) or cemented cup (vs. cementless cup; pooled odds ratio: 0.34, 95% CI: 0.13, 0.89). Recipients of cross-linked vs. conventional polyethylene cup liners experienced reduced femoral head penetration and revision. There was no impact of femoral stem fixation and cup shell design on implant survival rates. Evidence on mortality and complications (aseptic loosening, femoral fracture) was inconclusive. Conclusions: The majority of evidence was inconclusive due to poor reporting, missing data, or uncertainty in treatment estimates. The findings warrant cautious interpretation given the risk of bias (blinding, attrition), methodological limitations (small sample size, low event counts, short follow-up), and poor reporting. Long-term pragmatic RCTs are needed to allow for more definitive conclusions. Authors are encouraged to specify the minimal clinically important difference and power calculation for their primary outcome(s) as well CONSORT, PRISMA and STROBE guidelines to ensure better reporting and more reliable production and assessment of evidence