Internesting Movements of Loggerhead (Caretta caretta) Sea Turtles in Virginia, USA

Virginia is the northern most nesting region regularly utilized by loggerhead sea turtles (Caretta caretta) along the eastern coast of the United States. Along the southern shoreline of Virginia, between two and ten nests have been recorded annually since 1989 within Back Bay National Wildlife Refuge and False Cape State Park. Since 1992, the Virginia Institute of Marine Science (VIMS) has attached eight satellite transmitters to nesting loggerhead sea turtles in order to monitor their interesting movements and falVwinter migrations. VIMS has tracked the same nesting loggerhead three separate times during the 1993, 1995 and 1997 nesting seasons. This turtle has been observed to migrate into the Delaware Bay and Chesapeake Bay between nesting events and prior to her fall migrations. This project has demonstrated that nesting loggerhead sea turtles return to Virginia over subsequent seasons to nest, and utilize the Chesapeake and Delaware Bays as internesting and post-nesting habitat. Late season nesters tracked by VIMS have migrated south after their last recorded Virginia nesting event and have traveled along the coasts of North and South Carolina, in two cases establishing fall residency off the Carolinas. Three turtles have been tracked as far south as eastern Florida, with two entering the Gulf of Mexico before satellite transmissions ceased. The tracking data generated by this project suggest that the adult turtles utilizing Virginia as nesting habitat preferentially utilize either the orth Atlantic region or South Atlantic region as over-wintering habitat. Temperature ranges within which tagged turtles have remained range between 13° and 31 ° C. Three tagged turtles have remained in Virginia\u27s waters until sea surface temperatures dropped to between 13° and 16° C before migrating south

Evaluation of a Pilot of the Oregon Department of Transportation’s Ecodrive Program

Economical, ecological, and safe driving – eco-driving – is aimed at reducing fuel consumption and greenhouse gas emissions (Martin, Chan, & Shaheen, 2012). The adoption of energy-efficient driving styles and practices has been recognized as a means of reducing energy consumption, and estimates of energy savings attributed to eco-driving have been reported to range from 5% to as high as 20%, depending on the driving context (Barkenbus, 2010; Stillwater & Kurani, 2013; van der Voort, Dougherty & van Maareseveen, 2001). Eco-driving is being promoted in partnership among the Oregon Department of Transportation (ODOT) (Planning, Safety and Motor Carrier Division), the Department of Environmental Quality, the Oregon Department of Energy, and the Clean Cities Program (“EcoDriving Agency Partners”) to address fuel consumption in fleet drivers of light vehicles. The EcoDrive program was created to increase fuel efficiency in organizational fleets. The intent of the EcoDrive Program is to design and introduce eco-driving educational materials specifically targeted at drivers of light vehicles. The materials highlight the “Top EcoDrive Tips” and reinforce the relevant actions and benefits of eco-driving. The purpose of this report is to provide information about the implementation of the EcoDrive Program at three sites (publicsector organizations) within the state of Oregon and provide a preliminary evaluation of the program. In particular, this report will highlight how the EcoDrive Program impacted fleet drivers in terms of attitudes, knowledge, and behavior and how the drivers responded to the eco-driving materials. Further, we discuss recommendations for future implementations of the materials, highlighting possible organizational environments that are important for the success of the EcoDrive Program

Intellectual Property and International Trade

summary Historically, there has always been a clash of interests between originators and users of patents and other intellectual property rights. This paper provides an overview of the economic issues behind the intellectual property rights bargain domestically and internationally, now one of the key areas on negotiation in the current Uruguay round. Résumé La propriété intelletuelle et le commerce international Historiquement, il y a toujours eu un conflit d'intérêts entre les auteurs et les utilisateurs de brevets et autres droits de propriétés intellectuelles. Cet article présente une vue d'ensemble des problèmes économiques qui transparaissent dans le marché des droits de propriétés intellectuelles, au niveau national et international, actuellement l'un des secteurs?clé en cours de négotiations au sein du Cercle Uruguayien. Resumen Propiedad intelectual y comercio internacional Históricamente siempre ha existido un choque de intereses entre creadores y usuarios de patentes y otros derechos de propiedad intelectual. Este artículo proporciona una visión de los problemas económicos existentes en la negociación de los derechos de propiedad intelectual nacional e internacionalmente, que es una de las àreas claves en la negociación de la actual Rueda de Uruguay

Structural investigation of Fe silicide films grown by pulsed laser deposition

Pulsed laser deposition was used to grow epitaxial β‐FeSi2 films on Si(111) (1×1) and Si(111) (7×7) with the following epitaxial orientations: β‐FeSi2(001)//Si(111) with β‐FeSi2[010]//Si〈110〉 and three rotational variants. Silicide growth was influenced by substrate temperature and deposition rate, but not by the structure of the starting surface. Films containing both β‐FeSi2 and FeSi were formed at low substrate temperatures and high deposition rates, while films containing only β‐FeSi2 were formed at higher substrate temperatures and lower deposition rates. FeSi grains had the following epitaxial relationship to the Si substrate, FeSi(111)//Si(111) with FeSi(110)//Si(112). The microstructure of the silicide films varied with film thickness, as did the roughness at the silicide/Si interface. These results suggest that an Fe‐rich environment was created during the growth of the silicide films.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/70762/2/JAPIAU-76-4-2202-1.pd

Varied effects of algal symbionts on transcription factor NF-κB in a sea anemone and a coral: possible roles in symbiosis and thermotolerance

Many cnidarians, including the reef-building corals, undergo symbiotic mutualisms with photosynthetic dinoflagellate algae of the family Symbiodiniaceae. These partnerships are sensitive to temperature extremes, which cause symbiont loss and increased coral mortality. Previous studies have implicated host immunity and specifically immunity transcription factor NF-κB as having a role in the maintenance of the cnidarian-algal symbiosis. Here we have further investigated a possible role for NF-κB in establishment and loss of symbiosis in various strains of the anemone Exaiptasia (Aiptasia) and in the coral Pocillopora damicornis. Our results show that NF-κB expression is reduced in Aiptasia larvae and adults that host certain algae strains. Treatment of Aiptasia larvae with a known symbiosis-promoting cytokine, transforming growth factor β, also led to decreased NF-κB expression. We also show that aposymbiotic Aiptasia (with high NF-κB expression) have increased survival following infection with the pathogenic bacterium Serratia marcescens as compared to symbiotic Aiptasia (low NF-κB expression). Furthermore, a P. damicornis coral colony hosting Durusdinium spp. (formerly clade D) symbionts had higher basal NF-κB expression and decreased heat-induced bleaching as compared to two individuals hosting Cladocopium spp. (formerly clade C) symbionts. Lastly, genome-wide gene expression profiling and genomic promoter analysis identified putative NF-κB target genes that may be involved in thermal bleaching, symbiont maintenance, and/or immune protection in P. damicornis. Our results provide further support for the hypothesis that modulation of NF-κB and immunity plays a role in some, but perhaps not all, cnidarian-Symbiodiniaceae partnerships as well as in resistance to pathogens and bleaching.Accepted manuscrip

The stealth episome: suppression of gene expression on the excised genomic island PPHGI-1 from Pseudomonas syringae pv. phaseolicola

Pseudomonas syringae pv. phaseolicola is the causative agent of halo blight in the common bean, Phaseolus vulgaris. P. syringae pv. phaseolicola race 4 strain 1302A contains the avirulence gene avrPphB (syn. hopAR1), which resides on PPHGI-1, a 106 kb genomic island. Loss of PPHGI-1 from P. syringae pv. phaseolicola 1302A following exposure to the hypersensitive resistance response (HR) leads to the evolution of strains with altered virulence. Here we have used fluorescent protein reporter systems to gain insight into the mobility of PPHGI-1. Confocal imaging of dual-labelled P. syringae pv. phaseolicola 1302A strain, F532 (dsRFP in chromosome and eGFP in PPHGI-1), revealed loss of PPHGI-1::eGFP encoded fluorescence during plant infection and when grown in vitro on extracted leaf apoplastic fluids. Fluorescence-activated cell sorting (FACS) of fluorescent and non-fluorescent PPHGI-1::eGFP F532 populations showed that cells lost fluorescence not only when the GI was deleted, but also when it had excised and was present as a circular episome. In addition to reduced expression of eGFP, quantitative PCR on sub-populations separated by FACS showed that transcription of other genes on PPHGI-1 (avrPphB and xerC) was also greatly reduced in F532 cells harbouring the excised PPHGI-1::eGFP episome. Our results show how virulence determinants located on mobile pathogenicity islands may be hidden from detection by host surveillance systems through the suppression of gene expression in the episomal state

Living alone and mental health: parallel analyses in UK longitudinal population surveys and electronic health records prior to and during the COVID-19 pandemic

BACKGROUND: People who live alone experience greater levels of mental illness; however, it is unclear whether the COVID-19 pandemic had a disproportionately negative impact on this demographic. OBJECTIVE: To describe the mental health gap between those who live alone and with others in the UK prior to and during the COVID-19 pandemic. METHODS: Self-reported psychological distress and life satisfaction in 10 prospective longitudinal population surveys (LPSs) assessed in the nearest pre-pandemic sweep and three periods during the pandemic. Recorded diagnosis of common and severe mental illnesses between March 2018 and January 2022 in electronic healthcare records (EHRs) within the OpenSAFELY-TPP. FINDINGS: In 37 544 LPS participants, pooled models showed greater psychological distress (standardised mean difference (SMD): 0.09 (95% CI: 0.04; 0.14); relative risk: 1.25 (95% CI: 1.12; 1.39)) and lower life satisfaction (SMD: −0.22 (95% CI: −0.30; −0.15)) for those living alone pre-pandemic. This gap did not change during the pandemic. In the EHR analysis of c.16 million records, mental health conditions were more common in those who lived alone (eg, depression 26 (95% CI: 18 to 33) and severe mental illness 58 (95% CI: 54 to 62) more cases more per 100 000). For common mental health disorders, the gap in recorded cases in EHRs narrowed during the pandemic. CONCLUSIONS: People living alone have poorer mental health and lower life satisfaction. During the pandemic, this gap in self-reported distress remained; however, there was a narrowing of the gap in service use. CLINICAL IMPLICATIONS: Greater mental health need and potentially greater barriers to mental healthcare access for those who live alone need to be considered in healthcare planning

Political strategies of external support for democratization

Political strategies of external support to democratization are contrasted and critically examined in respect of the United States and European Union. The analysis begins by defining its terms of reference and addresses the question of what it means to have a strategy. The account briefly notes the goals lying behind democratization support and their relationship with the wider foreign policy process, before considering what a successful strategy would look like and how that relates to the selection of candidates. The literature's attempts to identify strategy and its recommendations for better strategies are compared and assessed. Overall, the article argues that the question of political strategies of external support for democratization raises several distinct but related issues including the who?, what?, why?, and how? On one level, strategic choices can be expected to echo the comparative advantage of the "supporter." On a different level, the strategies cannot be divorced from the larger foreign policy framework. While it is correct to say that any sound strategy for support should be grounded in a theoretical understanding of democratization, the literature on strategies reveals something even more fundamental: divergent views about the nature of politics itself. The recommendations there certainly pinpoint weaknesses in the actual strategies of the United States and Europe but they have their own limitations too. In particular, in a world of increasing multi-level governance strategies for supporting democratization should go beyond preoccupation with just an "outside-in" approach

A Prospective Randomized Crossover Trial of Systemic Chemotherapy in Patients with Low-Grade Mucinous Appendiceal Adenocarcinoma

View full abstracthttps://openworks.mdanderson.org/leading-edge/1048/thumbnail.jp

Independent and population-specific association of risk variants at the IRGM locus with Crohn's disease

DNA polymorphisms in a region on chromosome 5q33.1 which contains two genes, immunity related GTPase related family, M (IRGM) and zinc finger protein 300 (ZNF300), are associated with Crohn's disease (CD). The deleted allele of a 20 kb copy number variation (CNV) upstream of IRGM was recently shown to be in strong linkage disequilibrium (LD) with the CD-associated single nucleotide polymorphisms and is itself associated with CD (P < 0.01). The deletion was correlated with increased or reduced expression of IRGM in transformed cells in a cell line-dependent manner, and has been proposed as a likely causal variant. We report here that small insertion/deletion polymorphisms in the promoter and 5′ untranslated region of IRGM are, together with the CNV, strongly associated with CD (P = 1.37 × 10−5 to 1.40 × 10−9), and that the CNV and the 5′-untranslated region variant −308(GTTT)5 contribute independently to CD susceptibility (P = 2.6 × 10−7 and P = 2 × 10−5, respectively). We also show that the CD risk haplotype is associated with a significant decrease in IRGM expression (P < 10−12) in untransformed lymphocytes from CD patients. Further analysis of these variants in a Japanese CD case-control sample and of IRGM expression in HapMap populations revealed that neither the IRGM insertion/deletion polymorphisms nor the CNV was associated with CD or with altered IRGM expression in the Asian population. This suggests that the involvement of the IRGM risk haplotype in the pathogenesis of CD requires gene-gene or gene-environment interactions which are absent in Asian populations, or that none of the variants analysed are causal, and that the true causal variants arose after the European-Asian spli