Massachusetts communities at risk: a report from the Municipal Finance Task Force

Results and recommendations from an analysis of municipal finances and local aid during the period 1981 to 2005.Municipal finance - Massachusetts

From Discrimination: A Journey to Justice and Understanding

There have been milestones toward understanding in the Civil Rights journey . Do these milestones reflect an advance in understanding and respect, or have fears, ignorances, and outright bigotry forged a wall against acceptance of the Afro-American into mainstream America? An area in the Northeast County of St. Louis, Missouri was selected for this study as it encompassed 1036 residential homes . The area integrated starting about 1978. From surveys conducted by the Chairman of the Board of Trustees of this subdivision, in 1991 and 1992, it was determined that 63\u27l. of the families now are Afro-American, 37% are Caucasian or other. A questionnaire listing twenty questions pertaining to understanding, acceptance, feelings toward others of opposite race, was submitted to some residents and former residents. The variable to be measured was labeled Afro-American Caucasian Understanding (ACU). It was determined beforehand that the sample group of thirty-two be equally apportioned to Afro-American and to Caucasian. The survey was conducted with the assistance of the Chairman of the Board of Trustees who was an Afro-American male, and a Caucasian female member of the church within the area working with a Ladies Guild . This paper touches many facets of everyday existence including groups of school age individuals, entertainers, ball players, business persons, political persons, church people . Results of the survey indicated that there has been an increased understanding among the two cultures as measured against what might be expected from a Likert mean. Given a normal distribution, using the Likert scale with scorings at random one to five, on a twenty question survey, one would expect a mean score of 60 . The minimum score would be twenty, maximum score would be one hundred . The tables submitted gave information supporting a positive measurement toward understanding

Contracts

Covers cases on mutual assent—formation of construction subcontracts—use of subcontractor\u27s bid as acceptance

Trusts

Covers cases on testamentary trusts—violations of the rule against perpetuitities—effect of saving clause (Hamill) and on bank deposits as tentative or Totten trusts (Fraser)

Transcriptome analysis of porcine M. semimembranosus divergent in intramuscular fat as a consequence of dietary protein restriction

peer-reviewedBackground: Intramuscular fat (IMF) content is positively correlated with aspects of pork palatability, including flavour, juiciness and overall acceptability. The ratio of energy to protein in the finishing diet of growing pigs can impact on IMF content with consequences for pork quality. The objective of this study was to compare gene expression profiles of Musculus semimembranosus (SM) of animals divergent for IMF as a consequence of protein dietary restriction in an isocaloric diet. The animal model was derived through the imposition of low or high protein diets during the finisher stage in Duroc gilts. RNA was extracted from post mortem SM tissue, processed and hybridised to Affymetrix porcine GeneChip® arrays. Results: IMF content of SM muscle was increased on the low protein diet (3.60 ± 0.38% versus 1.92 ± 0.35%). Backfat depth was also greater in animals on the low protein diet, and average daily gain and feed conversion ratio were lower, but muscle depth, protein content and moisture content were not affected. A total of 542 annotated genes were differentially expressed (DE) between animals on low and high protein diets, with 351 down-regulated and 191 up-regulated on the low protein diet. Transcript differences were validated for a subset of DE genes by qPCR. Alterations in functions related to cell cycle, muscle growth, extracellular matrix organisation, collagen development, lipogenesis and lipolysis, were observed. Expression of adipokines including LEP, TNFα and HIF1α were increased and the hypoxic stress response was induced. Many of the identified transcriptomic responses have also been observed in genetic and fetal programming models of differential IMF accumulation, indicating they may be robust biological indicators of IMF content. Conclusion: An extensive perturbation of overall energy metabolism in muscle occurs in response to protein restriction. A low protein diet can modulate IMF content of the SM by altering gene pathways involved in lipid biosynthesis and degradation; however this nutritional challenge negatively impacts protein synthesis pathways, with potential consequences for growth.Department of Agriculture, Food and the Marine, Ireland - Food Institutional Research Measur

MiR-18a-5p Targets Connective Tissue Growth Factor Expression and Inhibits Transforming Growth Factor β2-Induced Trabecular Meshwork Cell Contractility

Increased trabecular meshwork (TM) cell and tissue contractility is a driver of the reduced outflow facility and elevation of intraocular pressure (IOP) associated with primary open-angle glaucoma (POAG). Connective tissue growth factor (CTGF) is an established mediator of TM cell contractility, and its expression is increased in POAG due to transforming growth factor β 2 (TGFβ2) signalling. Inhibiting CTGF upregulation using microRNA (miRNA) mimetics could represent a new treatment option for POAG. A combination of in silico predictive tools and a literature review identified a panel of putative CTGF-targeting miRNAs. Treatment of primary human TM cells with 5 ng/mL TGFβ2 for 24 h identified miR-18a-5p as a consistent responder, being upregulated in cells from five different human donors. Transfection of primary donor TM cells with 20 nM synthetic miR-18a-5p mimic reduced TGFβ2-induced CTGF protein expression, and stable lentiviral-mediated overexpression of this miRNA reduced TGFβ2-induced contraction of collagen gels. Together, these findings identify miR-18a-5p as a mediator of the TGFβ2 response and a candidate therapeutic agent for glaucoma via its ability to inhibit CTGF-associated increased TM contractility

The Yield of Essential Oils in Melaleuca alternifolia (Myrtaceae) Is Regulated through Transcript Abundance of Genes in the MEP Pathway

Medicinal tea tree (Melaleuca alternifolia) leaves contain large amounts of an essential oil, dominated by monoterpenes. Several enzymes of the chloroplastic methylerythritol phosphate (MEP) pathway are hypothesised to act as bottlenecks to the production of monoterpenes. We investigated, whether transcript abundance of genes encoding for enzymes of the MEP pathway were correlated with foliar terpenes in M. alternifolia using a population of 48 individuals that ranged in their oil concentration from 39 -122 mg x g DM(-1). Our study shows that most genes in the MEP pathway are co-regulated and that the expression of multiple genes within the MEP pathway is correlated with oil yield. Using multiple regression analysis, variation in expression of MEP pathway genes explained 87% of variation in foliar monoterpene concentrations. The data also suggest that sesquiterpenes in M. alternifolia are synthesised, at least in part, from isopentenyl pyrophosphate originating from the plastid via the MEP pathway.The work was funded by an Australian Research Council Linkage grant to W.J.F. (LP110100184) with the active partnership of the Australian Tea Tree Industry Association (ATTIA) and a supplementary grant from the Rural Industries Research and Development Corporation. The funders had no role is study design, data collection and analysis, decision to publish, or preparation of the manuscript

Effect of Breed and Gender on Meat Quality of M. longissimus thoracis et lumborum Muscle from Crossbred Beef Bulls and Steers

peer-reviewedAbstract The objective of this study was to determine whether sire breed and/or castration had an effect on meat quality of M. longissimus thoracis et lumborum (LTL) muscle from crossbred bulls and steers and to investigate the relationship amongst the traits examined. Warner–Bratzler shear force (WBSF), intramuscular fat (IMF)%, cook-loss%, drip-loss%, colour (L*, a*, b*) and ultimate pH (upH) were determined in the LTL muscle from eight beef sire breeds representative of the Irish herd (Aberdeen Angus, Belgian Blue, Charolais, Hereford, Limousin, Parthenaise, Salers and Simmental). The results indicate that IMF%, cook-loss% and drip-loss% were associated with breed (p < 0.05); while WBSF, IMF% and cook-loss% differ between genders (p < 0.05). Steer LTL had a greater IMF% and exhibited reduced WBSF and cook-loss% in comparison to the bull LTL (p < 0.05). This study provides greater insight into how quality traits in beef are influenced by breed and gender and will support the industry to produce beef with consistent eating quality

Using Fine Resolution Orthoimagery and Spatial Interpolation to Rapidly Map Turf Grass in Suburban Massachusetts

This paper explores the use of spatial interpolative methods in conjunction with object based image analysis to estimate turf grass land cover quantity and allocation in Greater Boston, Massachusetts, USA. The goal is to learn how accurately turf grass can be estimated if only a limited portion of the study area is mapped. First, turf grass land cover is mapped at the 0.5 m resolution across the entire Plum Island Ecosystems (PIE) Long Term Ecological Research (LTER) site, a 1143-km2 area. Second, the turf grass map is aggregated into 120 m cells (N = 84,661). Third, a random sample of these 120 m cells are selected to generate an estimate of the unselected cells using four estimation methods - Inverse Distance Weighting, Kriging, Polygonal Interpolation, and Mean Estimation. The difference between known and estimated values is recorded using 120 m cell and census block group stratifications. This process is repeated 500 times for sample sizes of 2.5%, 5.0%, 7.5% and 10.0% of the study area, for a total of 2000 iterations. The average error statistics are reported by sample size, strata, and estimation method. Inverse distance weighting performed best in terms of total error across all sample sizes. It was found that by mapping only 2.5% of the study area, all four methods outperformed a recently published approach to estimating turf grass in terms of overall error

Effects of down-regulating ornithine decarboxylase upon putrescine-associated metabolism and growth in Nicotiana tabacum L.

Transgenic plants of Nicotiana tabacum L. homozygous for an RNAi construct designed to silence ornithine decarboxylase (ODC) had significantly lower concentrations of nicotine and nornicotine, but significantly higher concentrations of anatabine, compared with vector-only controls. Silencing of ODC also led to significantly reduced concentrations of polyamines (putrescine, spermidine and spermine), tyramine and phenolamides (caffeoylputrescine and dicaffeoylspermidine) with concomitant increases in concentrations of amino acids ornithine, arginine, aspartate, glutamate and glutamine. Root transcript levels of S-adenosyl methionine decarboxylase, S-adenosyl methionine synthase and spermidine synthase (polyamine synthesis enzymes) were reduced compared with vector controls, whilst transcript levels of arginine decarboxylase (putrescine synthesis), putrescine methyltransferase (nicotine production) and multi-drug and toxic compound extrusion (alkaloid transport) proteins were elevated. In contrast, expression of two other key proteins required for alkaloid synthesis, quinolinic acid phosphoribosyltransferase (nicotinic acid production) and a PIP-family oxidoreductase (nicotinic acid condensation reactions), were diminished in roots of odc-RNAi plants relative to vector-only controls. Transcriptional and biochemical differences associated with polyamine and alkaloid metabolism were exacerbated in odc-RNAi plants in response to different forms of shoot damage. In general, apex removal had a greater effect than leaf wounding alone, with a combination of these injury treatments producing synergistic responses in some cases. Reduced expression of ODC appeared to have negative effects upon plant growth and vigour with some leaves of odc-RNAi lines being brittle and bleached compared with vector-only controls. Together, results of this study demonstrate that ornithine decarboxylase has important roles in facilitating both primary and secondary metabolism in Nicotiana