On Communication

Everybody knows that communication is important, but nobody knows how to define it. The best scholars refer to it. Free-speech law protects it. But no one-no scholar or judge-has successfully captured it. Few have even tried. This is the first article to define communication under the law. In it, I explain why some activities-music, abstract painting, and parading-are considered communicative under the First Amendment, while others-sex, drugs, and subliminal advertising-are not. I argue that the existing theories of communication, which hold that communicative behaviors are expressive or convey ideas, fail to explain what is going on in free-speech cases. Instead, communication hinges on the free will of the recipient. By this I mean that communication occurs when Person A conveys a thought to Person B, and Person B freely chooses whether to accept that thought. An act is communicative, in other words, if the important change that A wants to make in B\u27s mind occurs only if B wills it to, as happens during an argument. Reconceptualizing communication in this way-as behaviors meant to change minds through the free will of the listener-would solve deep and persistent First Amendment problems. It would explain which behaviors are communicative and therefore potentially covered by the First Amendment. Adopting the free-will theory would clarify the analysis in historically muddled areas such as the First Amendment treatment of nude dancing. But it would also shed light on the law governing new forms of behavior such as publication of computer-programming code. More broadly, the free-will theory of communication can point us in new directions. We are used to thinking of communication in ways that don\u27t describe it, and these errors may keep us from recognizing new forms of communication as they develop. Applying the free-will theory of communication, I argue, will prepare us for technological changes that will make our old metaphors for communication obsolete

Microfluidics : the fur-free way towards personalised medicine in cancer therapy

Microfluidic technology has great potential for complementing and, in some instances, replacing the use of animal models in the testing of medicines and in developing personalised treatments for cancer patients. The maintenance of tissue in an in vivo-like state provides a platform upon which normal and diseased tissue biology can be investigated in a novel way. This review describes the use of microfluidic technology for the maintenance of tissue samples ex vivo and the current state of play for the use of this technology in the replacement of animal models, with a focus on cancer

Frequency over function : raised levels of CD127low/- regulatory T cells in the tumour microenvironment compared with the periphery of head and neck cancer patients

Objective: Regulatory T cells (Tregs) are known to infiltrate the tumour microenvironment of many cancers, including head and neck malignancies, and are thought to contribute to the host's impaired anti-tumour immune response. However, their immunosuppressive function remains poorly understood within the tumour microenvironment and this study aimed to address this. Methods: The frequency and suppressive capacity of two CD4?CD127low/- Treg populations, separated on the basis of different levels of CD25 expression (CD25inter and CD25high), from the tumour/node microenvironment and peripheral circulation of newly-presenting head and neck squamous cell carcinoma patients (n=19), were assessed using multicolour flow cytometry. Results: The proportion of Tregs (CD4?CD25high/?interCD127low/-) in the tumour/node microenvironment was significantly elevated compared with the peripheral circulation (p<0.001) and similar percentages were present in both the primary tumour and metastatic lymph node. The percentage of suppression induced by Tregs isolated from tumour associated nodes on the proliferation of nodal effector T cells was similar to that of peripheral Tregs on peripheral effector T cells. However, when the suppressive activity of both nodal and peripheral Tregs was compared on the same peripheral effectors, peripheral Tregs suppressed proliferation to a greater extent. Conclusion: This work shows that the recruitment and percentages of tumour infiltrating Tregs are key factors in modulating the immune environment of head and neck tumours

Allometric scaling of microbial fuel cells and stacks: The lifeform case for scale-up

© 2017 Elsevier B.V. This case study reports for the first time on the comparison between allometric scaling of lifeforms and scale-up of microbial fuel cell entities; enlarging individual units in volume, footprint and electrode surface area but also multiplying a static size/footprint and electrode surface area to scale-up by stacking. A study published in 2010 by DeLong etal. showed for the first time that Kleiber's law does not apply uniformly to all lifeforms, and that in fact growth rate for prokaryotes is superlinear, for protists is linear and for metazoa is sublinear. The current study, which is utilising data from previous experiments, is showing for the first time that for individual MFC units, which are enlarged, growth rate/power is sublinear, whereas for stacks this is superlinear

On-chip determination of C-reactive protein using magnetic particles in continuous flow

We demonstrate the application of a multilaminar flow platform, in which functionalized magnetic particles are deflected through alternating laminar flow streams of reagents and washing solutions via an external magnet, for the rapid detection of the inflammatory biomarker, C-reactive protein (CRP). The two-step sandwich immunoassay was accomplished in less than 60 s, a vast improvement on the 80−300 min time frame required for enzyme-linked immunosorbent assays (ELISA) and the 50 min necessary for off-chip magnetic particle-based assays. The combination of continuous flow and a stationary magnet enables a degree of autonomy in the system, while a detection limit of 0.87 μg mL−1 makes it suitable for the determination of CRP concentrations in clinical diagnostics. Its applicability was further proven by assaying real human serum samples and comparing those results to values obtained using standard ELISA tests

Dynamic evolution of anodic biofilm when maturing under different external resistive loads in microbial fuel cells. Electrochemical perspective

© 2018 The Authors Appropriate inoculation and maturation may be crucial for shortening the startup time and maximising power output of Microbial Fuel Cells (MFCs), whilst ensuring stable operation. In this study we explore the relationship between electrochemical parameters of MFCs matured under different external resistance (Rext) values (50 Ω - 10 kΩ) using non-synthetic fuel (human urine). Maturing the biofilm under the lower selected Rext results in improved power performance and lowest internal resistance (Rint), whereas using higher Rext results in increased ohmic losses and inferior performance. When the optimal load is applied to the MFCs following maturity, dependence of microbial activity on original Rext values does not change, suggesting an irreversible effect on the biofilm, within the timeframe of the reported experiments. Biofilm microarchitecture is affected by Rext and plays an important role in MFC efficiency. Presence of water channels, EPS and precipitated salts is distinctive for higher Rext and open circuit MFCs. Correlation analysis reveals that the biofilm changes most dynamically in the first 5 weeks of operation and that fixed Rext lefts an electrochemical effect on biofilm performance. Therefore, the initial conditions of the biofilm development can affect its long-term structure, properties and activity

Recent advancements in real-world microbial fuel cell applications

© 2018 Elsevier B.V. This short review focuses on the recent developments of the Microbial Fuel Cell (MFC) technology, its scale-up and implementation in real world applications. Microbial Fuel Cells produce (bio)energy from waste streams, which can reduce environmental pollution, but also decrease the cost of the treatment. Although the technology is still considered “new” it has a long history since its discovery, but it is only now that recent developments have allowed its implementation in real world settings, as a precursor to commercialisation