Trustworthiness and Authority of Scholarly Information in a Digital Age: Results of an International Questionnaire

An international survey of over 3600 researchers examined how trustworthiness and quality are determined for making decisions on scholarly reading, citing, and publishing and how scholars perceive changes in trust with new forms of scholarly communication. Although differences in determining trustworthiness and authority of scholarly resources exist among age groups and fields of study, traditional methods and criteria remain important across the board. Peer review is considered the most important factor for determining the quality and trustworthiness of research. Researchers continue to read abstracts, check content for sound arguments and credible data, and rely on journal rankings when deciding whether to trust scholarly resources in reading, citing, or publishing. Social media outlets and open access publications are still often not trusted, although many researchers believe that open access has positive implications for research, especially if the open access journals are peer reviewed

Suppression of Ca2+ syntillas increases spontaneous exocytosis in mouse adrenal chromaffin cells

A central concept in the physiology of neurosecretion is that a rise in cytosolic [Ca2+] in the vicinity of plasmalemmal Ca2+ channels due to Ca2+ influx elicits exocytosis. Here, we examine the effect on spontaneous exocytosis of a rise in focal cytosolic [Ca2+] in the vicinity of ryanodine receptors (RYRs) due to release from internal stores in the form of Ca2+ syntillas. Ca2+ syntillas are focal cytosolic transients mediated by RYRs, which we first found in hypothalamic magnocellular neuronal terminals. (scintilla, Latin for spark; found in nerve terminals, normally synaptic structures.) We have also observed Ca2+ syntillas in mouse adrenal chromaffin cells. Here, we examine the effect of Ca2+ syntillas on exocytosis in chromaffin cells. In such a study on elicited exocytosis, there are two sources of Ca2+: one due to influx from the cell exterior through voltage-gated Ca2+ channels, and that due to release from intracellular stores. To eliminate complications arising from Ca2+ influx, we have examined spontaneous exocytosis where influx is not activated. We report here that decreasing syntillas leads to an increase in spontaneous exocytosis measured amperometrically. Two independent lines of experimentation each lead to this conclusion. In one case, release from stores was blocked by ryanodine; in another, stores were partially emptied using thapsigargin plus caffeine, after which syntillas were decreased. We conclude that Ca2+ syntillas act to inhibit spontaneous exocytosis, and we propose a simple model to account quantitatively for this action of syntillas

A comparison of phase imaging and quantitative susceptibility mapping in the imaging of multiple sclerosis lesions at ultrahigh field

Objective The aim of this study was to compare the use of high-resolution phase and QSM images acquired at ultra-high field in the investigation of multiple sclerosis (MS) lesions with peripheral rings, and to discuss their usefulness for drawing inferences about underlying tissue composition. Materials and methods Thirty-nine Subjects were scanned at 7 T, using 3D T2*-weighted and T1-weighted sequences. Phase images were then unwrapped and filtered, and quantitative susceptibility maps were generated using a thresholded k-space division method. Lesions were compared visually and using a 1D profiling algorithm. Results Lesions displaying peripheral rings in the phase images were identified in 10 of the 39 subjects. Dipolar projections were apparent in the phase images outside of the extent of several of these lesions; however, QSM images showed peripheral rings without such projections. These projections appeared ring-like in a small number of phase images where no ring was observed in QSM. 1D profiles of six well-isolated example lesions showed that QSM contrast corresponds more closely to the magnitude images than phase contrast. Conclusions Phase images contain dipolar projections, which confounds their use in the investigation of tissue composition in MS lesions. Quantitative susceptibility maps correct these projections, providing insight into the composition of MS lesions showing peripheral rings

A Genetic Screen for Dihydropyridine (DHP)-Resistant Worms Reveals New Residues Required for DHP-Blockage of Mammalian Calcium Channels

Dihydropyridines (DHPs) are L-type calcium channel (Cav1) blockers prescribed to treat several diseases including hypertension. Cav1 channels normally exist in three states: a resting closed state, an open state that is triggered by membrane depolarization, followed by a non-conducting inactivated state that is triggered by the influx of calcium ions, and a rapid change in voltage. DHP binding is thought to alter the conformation of the channel, possibly by engaging a mechanism similar to voltage dependent inactivation, and locking a calcium ion in the pore, thereby blocking channel conductance. As a Cav1 channel crystal structure is lacking, the current model of DHP action has largely been achieved by investigating the role of candidate Cav1 residues in mediating DHP-sensitivity. To better understand DHP-block and identify additional Cav1 residues important for DHP-sensitivity, we screened 440,000 randomly mutated Caenorhabditis elegans genomes for worms resistant to DHP-induced growth defects. We identified 30 missense mutations in the worm Cav1 pore-forming (α1) subunit, including eleven in conserved residues known to be necessary for DHP-binding. The remaining polymorphisms are in eight conserved residues not previously associated with DHP-sensitivity. Intriguingly, all of the worm mutants that we analyzed phenotypically exhibited increased channel activity. We also created orthologous mutations in the rat α1C subunit and examined the DHP-block of current through the mutant channels in culture. Six of the seven mutant channels examined either decreased the DHP-sensitivity of the channel and/or exhibited significant residual current at DHP concentrations sufficient to block wild-type channels. Our results further support the idea that DHP-block is intimately associated with voltage dependent inactivation and underscores the utility of C. elegans as a screening tool to identify residues important for DHP interaction with mammalian Cav1 channels

Practical robust MIMO OFDM communication system for high-speed mobile communication

This thesis presents the design of a communication system (PRCS) which improves on all aspects of the current state of the art 4G communication system Long Term Evolution (LTE) including peak to average power ratio (PAPR), data reliability, spectral efficiency and complexity using the most recent state of the art research in the field combined with novel implementations. This research is relevant and important to the field of electrical and communication engineering because it provides benefits to consumers in the form of more reliable data with higher speeds as well as a reduced burden on hardware original equipment manufacturers (OEMs). The results presented herein show up to a 3 dB reduction in PAPR, less than 10-5 bit errors at 7.5 dB signal to noise ratio (SNR) using 4QAM, up to 3 times increased throughput in the uplink mode and 10 times reduced channel coding complexity

Tools for semi-automatic monitoring of industrial workflows

This paper describes a tool chain for monitoring complex workflows. Statistics obtained from automatic workflow monitoring in a car assembly environment assist in improving industrial safety and process quality. To this end, we propose automatic detection and tracking of humans and their activity in multiple networked cameras. The described tools offer human operators retrospective analysis of a huge amount of pre-recorded and analyzed footage from multiple cameras in order to get a comprehensive overview of the workflows. Furthermore, the tools help technical administrators in adjusting algorithms by letting the user correct detections (for relevance feedback) and ground truth for evaluation. Another important feature of the tool chain is the capability to inform the employees about potentially risky conditions using the tool for automatic detection of unusual scene