The integral and its generalizations

By Percy John Daniell, M.A. (Cambridge), Senior Wrangler and Rayleigh Prizeman of Cambridge University, Professor of Applied Mathematics at the Rice Institute

Regulation of expression of the glutamine synthetase GLN-[alpha] gene of phaseolus vulgaris L

The expression of the glutamine synthetase a subunit gene (gln-a) is known to be high in young tissues such as root tips, plumules developing nodules and developing leaves. An experiment using nodules were Fix- and began to senesce prematurely, suggested that gln-a and a phenylalanine ammonia-lyase gene (PAL 1) were co-induced. This phenomenon was further studied in various systems including nodules derived with Rhizobium mutants, cell cultures treated with fungal elicitors, wounded hypocotyls and leaves infected with compatible and incompatible strains of Pseudomonas phaseolicola. Expression of these two genes and in some cases the glutamine synthetase ß subunit gene (gln-ß) was observed to be related although experiments with short periods between samples suggested that the expression of the two glutamine synthetase genes was delayed with respect to the expression of the PAL 1 gene, the peak of expression occuring a few hours later. Work on cell cultures suggests that the stimulus for the induction of the two glutamine synthetase genes is possibly a product of the phenylpropanoid pathway although other stimuli present in both stress conditions and in young tissues may induce either of the two glutamine synthetase gene

Ascertaining the nature and timing of mire degradation : using palaeoecology to assist future conservation management in Northern England

This study was initiated under contract VT0419 from English Nature (Natural England) to CECQR. In 2015, additional funding was provided by the Yorkshire Peat Partnership, for 210Pb dating, to refine age–depth estimates. We thank the following for fieldwork co-ordination, logistics or field assistance: Miriam Baynes, Mike Sutcliffe, Martin Furness, Gez Marshall, Paul Duncan, Tim Page, Andrew Windrum, Craig Sandham and Jackie Smith. We thank the Editor and two anonymous reviewers for suggestions for improvements.Peer reviewedPublisher PD

Expression of an Antimicrobial Peptide via the Chloroplast Genome to Control Phytopathogenic Bacteria and Fungi

The antimicrobial peptide MSI-99, an analog of magainin 2, was expressed via the chloroplast genome to obtain high levels of expression in transgenic tobacco (Nicotiana tabacum var. Petit Havana) plants. Polymerase chain reaction products and Southern blots confirmed integration of MSI-99 into the chloroplast genome and achievement of homoplasmy, whereas northern blots confirmed transcription. Contrary to previous predictions, accumulation of MSI-99 in transgenic chloroplasts did not affect normal growth and development of the transgenic plants. This may be due to differences in the lipid composition of plastid membranes compared with the membranes of susceptible target microbes. In vitro assays with protein extracts from T1 and T2 plants confirmed that MSI-99 was expressed at high levels to provide 88% (T1) and 96% (T2) inhibition of growth against Pseudomonas syringae pv tabaci, a major plant pathogen. When germinated in the absence of spectinomycin selection, leaf extracts from T2 generation plants showed 96% inhibition of growth against P. syringae pv tabaci. In addition, leaf extracts from transgenic plants (T1) inhibited the growth of pregerminated spores of three fungal species, Aspergillus flavus, Fusarium moniliforme, and Verticillium dahliae, by more than 95% compared with non-transformed control plant extracts. In planta assays with the bacterial pathogen P. syringae pv tabaci resulted in areas of necrosis around the point of inoculation in control leaves, whereas transformed leaves showed no signs of necrosis, demonstrating high-dose release of the peptide at the site of infection by chloroplast lysis. In planta assays with the fungal pathogen, Colletotrichum destructivum, showed necrotic anthracnose lesions in non-transformed control leaves, whereas transformed leaves showed no lesions. Genetically engineering crop plants for disease resistance via the chloroplast genome instead of the nuclear genome is desirable to achieve high levels of expression and to prevent pollen-mediated escape of transgenes

Conservation and habitat restoration of moorland and bog in the UK uplands: A regional, paleoecological perspective

Conservation practice in the United Kingdom (UK) uplands has been strongly influenced by policy responses to the Convention on Biological Diversity (1992). The UK was the first nation to produce a national Biodiversity Action Plan (BAP)(Department for Environment, Food and Rural Affairs, 2007a, b; UKBAP, 2008); policy is now enshrined in Habitat Action Plans (65 HAPs), Species Action Plans (1149 SAPs) and Local Biodiversity Action Plans (innumerable LBAPs), plus European designations of Special Areas of Conservation (SACs

Soil nitrate reducing processes drivers, mechanisms for spatial variation, and significance for nitrous oxide production

The microbial processes of denitrification and dissimilatory nitrate reduction to ammonium (DNRA) are two important nitrate reducing mechanisms in soil, which are responsible for the loss of nitrate (NO− 3 ) and production of the potent greenhouse gas, nitrous oxide (N2O). A number of factors are known to control these processes, including O2 concentrations and moisture content, N, C, pH, and the size and community structure of nitrate reducing organisms responsible for the processes. There is an increasing understanding associated with many of these controls on flux through the nitrogen cycle in soil systems. However, there remains uncertainty about how the nitrate reducing communities are linked to environmental variables and the flux of products from these processes. The high spatial variability of environmental controls and microbial communities across small sub centimeter areas of soil may prove to be critical in determining why an understanding of the links between biotic and abiotic controls has proved elusive. This spatial effect is often overlooked as a driver of nitrate reducing processes. An increased knowledge of the effects of spatial heterogeneity in soil on nitrate reduction processes will be fundamental in understanding the drivers, location, and potential for N2O production from soils

European normative values for physical fitness in children and adolescents aged 9–17 years: results from 2 779 165 Eurofit performances representing 30 countries

OBJECTIVE: To develop sex-specific and age-specific normative values for the nine Eurofit tests in European children and adolescents aged 9-17 years. METHODS: A systematic review was undertaken to identify papers that explicitly reported descriptive results for at least one of nine Eurofit tests (measuring balance, muscular strength, muscular endurance, muscular power, flexibility, speed, speed-agility and cardiorespiratory fitness (CRF)) on children and adolescents. Data were included on apparently healthy (free from known disease/injury) children and adolescents aged 9-17 years. Following harmonisation for methodological variation where appropriate, pseudodata were generated using Monte Carlo simulation, with population-weighted sex-specific and age-specific normative centiles generated using the Lambda Mu Sigma (LMS) method. Sex-specific and age-specific differences were expressed as standardised differences in means, with the percentage of children and adolescents with healthy CRF estimated at the sex-age level. RESULTS: Norms were displayed as tabulated centiles and as smoothed centile curves for the nine Eurofit tests. The final dataset included 2 779 165 results on children and adolescents from 30 European countries, extracted from 98 studies. On average, 78% of boys (95% CI 72% to 85%) and 83% of girls (95% CI 71% to 96%) met the standards for healthy CRF, with the percentage meeting the standards decreasing with age. Boys performed substantially (standardised differences \u3e0.2) better than girls on muscular strength, muscular power, muscular endurance, speed-agility and CRF tests, but worse on the flexibility test. Physical fitness generally improved at a faster rate in boys than in girls, especially during the teenage years. CONCLUSION: This study provides the largest and most geographically representative sex-specific and age-specific European normative values for children and adolescents, which have utility for health and fitness screening, profiling, monitoring and surveillance

Album de musique anglaise du 17ième siècle

Sammelwerk mit englischer Musik des 17. Jahrhunderts für Violine oder Viola, mit Baß. Enthält 24 Kompositionen von Christopher Simpson, Anthony Poole, John Jenkins u.a. Zeitgenössische Handschrift. 1. Poole, Anthony : Battle and triumph 2. Poole, Anthony : Division [Einheitssacht.: Divertimenti, Vl B, a-Moll] 3. Simpson, Christopher : Prelude [Einheitssacht.: Preludes, Vl, D-Dur] 4. Pollwheel, Francis : Division 5. Nortcombe, Daniell : Division 6. Poole, Anthony : Chacone 7. Young, Peter : Division 8. Withey, John : Division 9. Poole, Anthony : Chacone 10. Poole, Anthony : Chacone 11.-13. herausgerissen 14. Poole, Anthony : Division 15. Jenkins, John : 1. Division 16. Jenkins, John : 2. Division 17. Norcombe, Daniell : 3. Division 18. Simpson, Christopher : 1. Division 19. Simpson, Christopher : 2. Division 20. Simpson, Christopher : 3. Division 21. Simpson, Christopher : Division 22. Poole, Anthony : Division 23. Baltzar, Thomas : Allmand 24. Baltzar, Thomas : Coran