Evaluering af Istedgade: Bedre forhold for cyklister

Københavns Kommune har gennemført en større ombygning af Istedgade for blandt andet at skabe bedre cykelforhold. Målet med projektet har været at omdanne den eksisterende bydelsgade til en strøggade med fokus på handelsliv, busser, fodgængere og cyklister, men uden gennemgående cykelstier. Projektet blev udviklet og gennemført i en proces med stor inddragelse af borgere og interessenter, og med særligt fokus på Københavns Kommunes trafikale og byrumsmæssige mål og strategier. Projektets sidste etape blev taget i brug i sommeren 2015. Den samlede løsning for Istedgade har dermed været benyttet i godt et halvt år. Københavns Kommune har derfor ønsket at opsamle de foreløbige erfaringer, og evaluere projektet. Evalueringen undersøger i hvilken grad projektet har indfriet de ønskede mål og effekter, for på det grundlag, at kunne drage erfaringer som kan bidrage til kommende projekter. Denne artikel beskriver resultatet af erfaringsopsamling og evaluering, som er udført af Københavns Kommune i samarbejde med Via Trafik Rådgivning A/S og Megafon

Spit-systems – an overlooked target in hydrocarbon exploration: the Holocene to Recent Skagen Odde, Denmark

Well-constrained depositional models are essential for successful exploration and field development. The Skagen spitsystem offers a unique possibility for the establishment of a depositional model constrained by excellent outcrops, well-defined palaeogeography, good age control and detailed observations on hydrodynamics and morphology of the prograding part of the spit-system. The model offers a supplementary interpretation of shallow marine sandstones to the existing delta and linear shoreface models. The sand-dominated Skagen spit-system is c. 22 km long, 4 km wide and up to 35 m thick, with a sand volume of c. 2.2 km3. If filled with oil, this system would contain 0.6 km3 corresponding to 3.8 x 109 barrels assuming a porosity of 30% and an oil saturation of 90%. This is comparable in size with the largest Danish oil field (the Dan field), in the North Sea. Reservoir models for isolated linear ‘offshore’ sandstone bodies have been controversial for many years. Their size and internal indications of palaeocurrent directions are similar to those of the spit-system model, and this model may therefore be applicable for some of these bodies

Body Fluid Identification in Samples Collected after Intimate and Social Contact: A Comparison of Two mRNA Profiling Methods and the Additional Information Gained by cSNP Genotypes

The ability to associate a contributor with a specific body fluid in a crime stain can aid casework investigation. The detection of body fluids combined with DNA analyses may supply essential information, but as the two tests are independent, they may not be associated. Recently, the analysis of coding region SNPs (cSNPs) within the RNA transcript has been proven to be a promising method to face this challenge. In this study, we performed targeted RNA sequencing of 158 samples (boxershorts, fingernail swabs and penile swabs) collected from 12 couples at different time points post-intimate contact and after non-intimate contact, using the Ion S5™ System and BFID-cSNP-6F assay. The aim of the study was to compare the performance of the MPS and CE methods in the detection of mRNA markers, and to associate body fluids with contributors by their cSNP genotypes. The results of the study show a lower success rate in the detection of vaginal mucosa by the MPS compared to the CE method. However, the additional information obtained with the cSNP genotypes could successfully associate body fluids with contributors in most cases

Inflammatory tissue reactions around aseptically loose cemented hip prostheses: A retrieval study of the Spectron EF stem with Reflection All-Poly acetabular cup

The cemented Spectron EF stem in combination with the cemented non-crosslinked Reflection All-Poly cup showed a high rate of mid-term aseptic loosening. However, the failure mechanisms are not fully known. We assessed the inflammatory tissue reactions and wear particles in periprosthetic tissues, implant wear and blood metal ion levels in 28 patients with failed implants. Histological analysis showed a macrophage pre-dominant pattern with randomly distributed lymphocytes, with various amounts of neutrophils and giant cells. The number of different cell types in the tissue samples from patients in the cup group and in the stem group was similar. Wear particles, mainly ZrO2, CoCrMo, and polyethylene particles of different sizes and shapes, were associated with macrophages/giant cells, and total particle load/mm2 was higher in cases of stem loosening. The Spectron EF stems were heavily worn, abraded, and polished. Stem abrasion correlated with metal ion concentrations in blood. The median polyethylene wear rate of the Reflection cups was 0.23 mm/year. The high proximal roughness of the Spectron EF stem resulted in excessive cement wear during loosening. The resulting inflammatory tissue responses to the degradation products both from the cup and the stem led to massive osteolysis and subsequent implant loosening.publishedVersio

Evolution of the Hepatitis E virus hypervariable region

The presence of a hypervariable (HVR) region within the genome of hepatitis E virus (HEV) remains unexplained. Previous studies have described the HVR as a proline-rich spacer between flanking functional domains of the ORF1 polyprotein. Others have proposed that the region has no function, that it reflects a hypermutable region of the virus genome, that it is derived from the insertion and evolution of host sequences or that it is subject to positive selection. This study attempts to differentiate between these explanations by documenting the evolutionary processes occurring within the HVR. We have measured the diversity of HVR sequences within acutely infected individuals or amongst sequences derived from epidemiologically linked samples and, surprisingly, find relative homogeneity amongst these datasets. We found no evidence of positive selection for amino acid substitution in the HVR. Through an analysis of published sequences, we conclude that the range of HVR diversity observed within virus genotypes can be explained by the accumulation of substitutions and, to a much lesser extent, through deletions or duplications of this region. All published HVR amino acid sequences display a relative overabundance of proline and serine residues that cannot be explained by a local bias towards cytosine in this part of the genome. Although all published HVRs contain one or more SH3-binding PxxP motifs, this motif does not occur more frequently than would be expected from the proportion of proline residues in these sequences. Taken together, these observations are consistent with the hypothesis that the HVR has a structural role that is dependent upon length and amino acid composition, rather than a specific sequence

Structure of the ALS Mutation Target Annexin A11 Reveals a Stabilising N-Terminal Segment

The functions of the annexin family of proteins involve binding to Ca2+, lipid membranes, other proteins, and RNA, and the annexins share a common folded core structure at the C terminus. Annexin A11 (AnxA11) has a long N-terminal region, which is predicted to be disordered, binds RNA, and forms membraneless organelles involved in neuronal transport. Mutations in AnxA11 have been linked to amyotrophic lateral sclerosis (ALS). We studied the structure and stability of AnxA11 and identified a short stabilising segment in the N-terminal end of the folded core, which links domains I and IV. The crystal structure of the AnxA11 core highlights main-chain hydrogen bonding interactions formed through this bridging segment, which are likely conserved in most annexins. The structure was also used to study the currently known ALS mutations in AnxA11. Three of these mutations correspond to buried Arg residues highly conserved in the annexin family, indicating central roles in annexin folding. The structural data provide starting points for detailed structure–function studies of both full-length AnxA11 and the disease variants being identified in ALS.publishedVersio