Multiple sequence alignment of psba(ncr) DNA sequences from Symbiodinium thermophilum and closely related Symbiodinium spp.

This file is a multiple sequence alignment of partial chloroplast psbA non coding region genes from Symbiodinium thermophilum and other closely related species of Symbiodinium. It is in a FASTA format. The first 22 Symbiodinium sequences were obtained from algae within Porites spp. corals from the Persian/Arabian gulf. The host identity, specific collection location and host species for each sample are detailed in the supplementary materials of the associated paper. The alignment was implemented using ClustalW but heavily modified manually. Reference sequences are included in the alignment. These are available in Genbank as detailed in the associated paper

ILM for Dryad

Raw data used to produce figures in the manuscript

Suspension Images

Unprocessed chlorophyll emission images at different excitation wavelengths in zooxanthellae suspension

Discosoma Images

Unprocessed chlorophyll emission images at different excitation wavelengths in Discosoma

amilFP597 promoter

Analysis of the amilFP597 promoter copy ratios in six A. millepora morphs with different levels of redness

Abs spectra of A millepora

Absorption spectra of clarified tissue extracts of the HR and LR A. millepora morphs. The absorption spectrum of purified recombinant amilFP597 normalised to the peak value of the HR spectrum is included for comparison

Sequences RFP full length genes

All raw sequences used to reconstruct the amilFP597 gene in A. millepora.Full-length sequences of indel (-) and indel (+) promoter variants of the amilFP597 gene were obtained for the MR morph. These sequences, extending from the promoter region to the 3’UTR, were produced by joining the sequences of two overlapping PCR products covering the 5’ region [promoter to exon 3 amplified using primers pRFPlargeF (+) or pRFPsmallF (-) and RFP SP1] and the 3’ region (intron 2 to 3’UTR amplified using primers RFP_I2-3’U_F and RFP_I2-3’U_R2). Sequence differences in the overlap between the 5’ and 3’ region fragments were used to assign the latter to either the indel (+) or indel (-) promoter variant genes. The promoter-exon 3 and intron 2-3’UTR fragments were cloned and sequenced on both strands using vector primers and by primer walking. The assembled sequences have been submitted to GenBank as accessions KC818413 [indel (-) gene] and KC818414 [indel (+) gene]

amilFP597 exon 3 sequences

The chromophore coding sequences of RFP-related genes of A. millepora were amplified using genomic DNA of LR, MR and HR morphs as the template with primers designed to conserved regions within exon 3

Spectroscopic characteristics of GFP-like proteins

All spectroscopic data utilised for the characterisation of amilFP597, amilCP506, amilCP564 and amilFP60

amilFP597 copies

Copy numbers of amilFP597 variant genes determined by semi-quantitative PCR amplification of a conserved genomic exon 3 fragment and by diagnostic restriction analysis with ApeK1