Mass distribution and structural parameters of Small Magellanic Cloud star clusters

In this work, we estimate, for the first time, the total masses and mass function (MF) slopes of a sample of 29 young and intermediate-age Small Magellanic Cloud (SMC) clusters from CCD Washington photometry. We also derive age, interstellar reddening and structural parameters for most of the studied clusters by employing a statistical method to remove the unavoidable field star contamination. Only these 29 clusters out of 68 originally analysed cluster candidates present stellar overdensities and coherent distribution in their colour–magnitude diagrams compatible with the existence of a genuine star cluster. We employed simple stellar population models to derive general equations for estimating the cluster mass based only on its age and integrated light in the B, V, I, C and T1 filter. These equations were tested against mass values computed from luminosity functions, showing an excellent agreement. The sample contains clusters with ages between 60 Myr and 3 Gyr and masses between 300 and 3000 M ⊙ distributed between ∼0$$_{.}^{\circ}5 and ∼2° from the SMC optical centre. We determined MF slopes for 24 clusters, of which 19 have slopes compatible with that of Kroupa's initial mass function (α = 2.3 ± 0.7), considering the uncertainties. The remaining clusters – H86-188, H86-190, K47, K63 and NGC 242 – showed flatter MFs. Additionally, only clusters with masses lower than ∼1000 M ⊙ and flatter MF were found within ∼0$ $_{.}^{\circ}6 from the SMC rotational centre.Fil: Maia, F.F.S.. Universidade Federal do Minas Gerais; BrasilFil: Piatti, Andres Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cordoba. Observatorio Astronomico de Cordoba; ArgentinaFil: Santos Jr., João F. C.. Universidade Federal do Minas Gerais; Brasi

A Search for Old Star Clusters in the Large Magellanic Cloud

We report the first results of a color-magnitude diagram survey of 25 candidate old LMC clusters. For almost all of the sample, it was possible to reach the turnoff region, and in many clusters we have several magnitudes of the main sequence. Age estimates based on the magnitude difference $\delta T_1$ between the giant branch clump and the turnoff revealed that no new old clusters were found. The candidates turned out to be of intermediate age (1-3 Gyr) We show that the apparently old ages as inferred from integrated UBV colors can be explained by a combination of stochastic effects produced by bright stars and by photometric errors for faint clusters lying in crowded fields. The relatively metal poor candidates from the CaII triplet spectroscopy also turned out to be of intermediate age. This, combined with the fact that they lie far out in the disk, yields interesting constraints regarding the formation and evolution of the LMC disk. We also study the age distribution of intermediate age and old clusters This homogeneous set of accurate relative ages allows us to make an improved study of the history of cluster formation/destruction for ages $>1$Gyr. We confirm previous indications that there was apparently no cluster formation in the LMC during the period from 3-8 Gyr ago, and that there was a pronounced epoch of cluster formation beginning 3 Gyrs ago that peaked at about 1.5 Gyrs ago. Our results suggest that there are few, if any, genuine old clusters in the LMC left to be found.Comment: LaTeX, to be published in Nov. 1997 Astronomical Journa

Open Cluster Characterization via Cross-Correlation with Spectral Library

We present a characterization method based on spectral cross-correlation to obtain the physical parameters of the controversial stellar aggregate ESO442-SC04. The data used was obtained with GMOS at Gemini-South telescope including 17 stars in the central region of the ob ject and 6 standard-stars. FXCOR was used in an iterative process to obtain self-consistent radial velocities for the standard-stars and averaged radial velocities for the science spectra. Spectral types, effective temperature, suface gravity and metallicities parameters were determined using FXCOR to correlate cluster spectra with ELODIE spectral library and selecting the best correlation matches using the Tonry and Davis Ratio (TDR). Analysis of the results suggests that the stars in ESO442-SC04 are not bound and therefore they do not constitute a physical system.Comment: 4-page paper from IAU symposium 266. Contains 3 eps figures and IAU document class file 'iau.cls

Novel Prostate Specific Antigen plastic antibody designed withcharged binding sites for an improved protein binding and itsapplication in a biosensor of potentiometric transduction

This work shows that the synthesis of protein plastic antibodies tailored with selected charged monomersaround the binding site enhances protein binding. These charged receptor sites are placed over a neutralpolymeric matrix, thus inducing a suitable orientation the protein reception to its site. This is confirmed bypreparing control materials with neutral monomers and also with non-imprinted template. This concepthas been applied here to Prostate Specific Antigen (PSA), the protein of choice for screening prostate can-cer throughout the population, with serum levels >10 ng/mL pointing out a high probability of associatedcancer.Protein Imprinted Materials with charged binding sites (C/PIM) have been produced by surfaceimprinting over graphene layers to which the protein was first covalently attached. Vinylben-zyl(trimethylammonium chloride) and vinyl benzoate were introduced as charged monomers labellingthe binding site and were allowed to self-organize around the protein. The subsequent polymerizationwas made by radical polymerization of vinylbenzene. Neutral PIM (N/PIM) prepared without orientedcharges and non imprinted materials (NIM) obtained without template were used as controls.These materials were used to develop simple and inexpensive potentiometric sensor for PSA. Theywere included as ionophores in plasticized PVC membranes, and tested over electrodes of solid or liq-uid conductive contacts, made of conductive carbon over a syringe or of inner reference solution overmicropipette tips. The electrodes with charged monomers showed a more stable and sensitive response,with an average slope of -44.2 mV/decade and a detection limit of 5.8 × 10−11mol/L (2 ng/mL). The cor-responding non-imprinted sensors showed lower sensitivity, with average slopes of -24.8 mV/decade.The best sensors were successfully applied to the analysis of serum, with recoveries ranging from 96.9to 106.1% and relative errors of 6.8%

Development of fast dissolving polymer-based microneedles for delivery of an antigenic melanoma cell membrane

Delivery of cancer cell membranes (CM) is a new approach for the activation of the immune system and the induction of immunotherapy of cancer. Local delivery of melanoma CM into skin can induce efficient immune stimulation of antigen presenting cells (APCs), such as dendritic cells. In the current study, fast dissolving microneedles (MNs) were developed for the delivery of melanoma B16F10 CM. Two polymers were tested for the fabrication of MNs: poly(methyl vinyl ether-co-maleic acid) (PMVE-MA) and hyaluronic acid (HA). The incorporation of CM in MNs was achieved through coating of the MNs using a multi-step layering procedure or the micromolding technique. The CM loading and its stabilization were improved by adding sugars (sucrose and trehalose) and a surfactant (Poloxamer 188), respectively. In an ex vivo experiment, both PMVE-MA and HA showed fast dissolutions (<30 s) after insertion into porcine skin. However, HA-MA showed better mechanical properties, namely improved resistance to fracture when submitted to a compression force. Overall, a B16F10 melanoma CM-dissolving MN system was efficiently developed as a promising device suggesting further studies in immunotherapy and melanoma applications

Co-formability, solubility enhancement and stability of Olanzapine co-amorphous systems produced with different co-formers

Abstract of the poster presented at the 4th International Congress of CiiEM - "Health, Well-being and Ageing in the XXI Century." 2-5 June 2019, Campus Egas Moniz, Monte de Caparica, PortugalN/

Development of fast dissolving polymer-based microneedles for delivery of an antigenic melanoma cell membrane

Delivery of cancer cell membranes (CM) is a new approach for the activation of the immune system and the induction of immunotherapy of cancer. Local delivery of melanoma CM into skin can induce efficient immune stimulation of antigen presenting cells (APCs), such as dendritic cells. In the current study, fast dissolving microneedles (MNs) were developed for the delivery of melanoma B16F10 CM. Two polymers were tested for the fabrication of MNs: poly(methyl vinyl ether-co-maleic acid) (PMVE-MA) and hyaluronic acid (HA). The incorporation of CM in MNs was achieved through coating of the MNs using a multi-step layering procedure or the micromolding technique. The CM loading and its stabilization were improved by adding sugars (sucrose and trehalose) and a surfactant (Poloxamer 188), respectively. In an ex vivo experiment, both PMVE-MA and HA showed fast dissolutions (<30 s) after insertion into porcine skin. However, HA-MA showed better mechanical properties, namely improved resistance to fracture when submitted to a compression force. Overall, a B16F10 melanoma CM-dissolving MN system was efficiently developed as a promising device suggesting further studies in immunotherapy and melanoma applications

Characterization and stability of co-amorphous systems containing Olanzapine and sulfonic acids

Abstract of the communication presented at the 4th International Congress of CiiEM - "Health, Well-being and Ageing in the XXI Century." 2-5 June 2019, Campus Egas Moniz, Monte de Caparica, PortugalN/

Development of fast dissolving polymer-based microneedles for delivery of an antigenic melanoma cell membrane

Delivery of cancer cell membranes (CM) is a new approach for the activation of the immune system and the induction of immunotherapy of cancer. Local delivery of melanoma CM into skin can induce efficient immune stimulation of antigen presenting cells (APCs), such as dendritic cells. In the current study, fast dissolving microneedles (MNs) were developed for the delivery of melanoma B16F10 CM. Two polymers were tested for the fabrication of MNs: poly(methyl vinyl ether-co-maleic acid) (PMVE-MA) and hyaluronic acid (HA). The incorporation of CM in MNs was achieved through coating of the MNs using a multi-step layering procedure or the micromolding technique. The CM loading and its stabilization were improved by adding sugars (sucrose and trehalose) and a surfactant (Poloxamer 188), respectively. In an ex vivo experiment, both PMVE-MA and HA showed fast dissolutions (<30 s) after insertion into porcine skin. However, HA-MA showed better mechanical properties, namely improved resistance to fracture when submitted to a compression force. Overall, a B16F10 melanoma CM-dissolving MN system was efficiently developed as a promising device suggesting further studies in immunotherapy and melanoma applications

Development of fast dissolving polymer-based microneedles for delivery of an antigenic melanoma cell membrane

Delivery of cancer cell membranes (CM) is a new approach for the activation of the immune system and the induction of immunotherapy of cancer. Local delivery of melanoma CM into skin can induce efficient immune stimulation of antigen presenting cells (APCs), such as dendritic cells. In the current study, fast dissolving microneedles (MNs) were developed for the delivery of melanoma B16F10 CM. Two polymers were tested for the fabrication of MNs: poly(methyl vinyl ether-co-maleic acid) (PMVE-MA) and hyaluronic acid (HA). The incorporation of CM in MNs was achieved through coating of the MNs using a multi-step layering procedure or the micromolding technique. The CM loading and its stabilization were improved by adding sugars (sucrose and trehalose) and a surfactant (Poloxamer 188), respectively. In an ex vivo experiment, both PMVE-MA and HA showed fast dissolutions (<30 s) after insertion into porcine skin. However, HA-MA showed better mechanical properties, namely improved resistance to fracture when submitted to a compression force. Overall, a B16F10 melanoma CM-dissolving MN system was efficiently developed as a promising device suggesting further studies in immunotherapy and melanoma applications