Thermoresponsive Hydrogel Containing Viscum album Extract for Topic and Transdermal Use: Development, Stability and Cytotoxicity Activity

Viscum album L. (Santalaceae), also known as European mistletoe, is a semi-parasitic plant that grows on different host trees. Our group recently demonstrated the antitumoral activity of ethanolic V. album extracts in vitro, depending on the dose and the host tree, V. album ssp abietis from Abies alba being the most active extract. The goal of this work focused on the development of a new topical formulation containing V. album extracts, evaluation of in vitro toxicity and ex vivo skin permeation assays. The Poloxamer 407 hydrogel containing 5% of dry (VA_DEH) or aqueous (VA_AEH) extract presented dermal compatible pH and microbiological stability for 180 days. The hydrogels flow curve presented a non-linear relation, characteristic of non-Newtonian fluids, and the mean viscosity for the VA_DEH and VA_AEH was 372.5 ± 7.78 and 331.0 ± 2.83 Pa.s, respectively, being statistically different (Welch’s t test; p < 0.01). Additionally, WST-1 in vitro assays revealed a dose-dependent toxicity for both formulations and VA_DEH presented a higher activity than the VA_AEH. The promising cytotoxic potential of VA_DEH lead to the ex vivo skin permeation assay with 2.73 ± 0.19 µg/cm(2) of chlorogenic acid, which permeated at 8 h, showing a transdermal potential. These in vitro results support the idea that VA_DEH is a novel promising candidate for mistletoe therapy. Therefore, further in vivo and pre-clinical experiments should be performed to evaluate the safety and efficacy of this new dermic delivery system

O impacto da ultrassonografia no atendimento multidisciplinar na UTI

Introduction: The introduction of ultrasound in the Intensive Care Unit (ICU) represents a significant milestone, fostering substantial advancements in multidisciplinary practice and positively influencing the management of various critical conditions. The application of pulmonary ultrasound, in particular, emerges as a versatile and essential tool, directly impacting the quality of care provided. Methodology: A bibliographic search was conducted on the PUBMED platform, utilizing specific Mesh descriptors: Ultrasonography, Intensive Care Units, Patient Care Team. The search period spanned from 2013 to 2021, encompassing a total of 33 articles. Results: The application of ultrasound in the ICU has proven to be an extremely relevant tool for multidisciplinary care. Pulmonary ultrasound, when employed by intensivists, not only provides a precise assessment of the mentioned conditions but also plays a fundamental role in rapid decision-making and treatment optimization. Conclusion: Ultrasound emerges as a transformative tool in the ICU, shaping multidisciplinary practice and ushering in an era of more advanced and patient-centered intensive care. Its crucial role in swift decision-making and treatment optimization reinforces its position as an indispensable element in the toolkit of healthcare professionals in both the present and future ICU settings.Introdução: A introdução da ultrassonografia na Unidade de Terapia Intensiva (UTI) representa um marco significativo, promovendo avanços substanciais na prática multidisciplinar e influenciando positivamente o manejo de diversas condições críticas. A aplicação da ultrassonografia pulmonar, em particular, surge como uma ferramenta versátil e essencial, impactando diretamente a qualidade do atendimento prestado. Metodologia: A pesquisa bibliográfica foi conduzida na plataforma PUBMED, utilizando descritores Mesh específicos: Ultrasonography, Intensive Care Units, Patient Care Team. O período de busca abrangeu os anos de 2013 a 2021, totalizando 33 artigos. Resultado: Aplicação da ultrassonografia na UTI tem se mostrado uma ferramenta de extrema relevância para o atendimento multidisciplinar. A ultrassonografia pulmonar, quando utilizada pelos intensivistas, não apenas oferece uma avaliação precisa das condições mencionadas, mas também desempenha um papel fundamental na rápida tomada de decisões e na otimização do tratamento. Conclusão: A ultrassonografia emerge como uma ferramenta transformadora na UTI, moldando a prática multidisciplinar e promovendo uma era de cuidados intensivos mais avançados e centrados no paciente. Seu papel crucial na rápida tomada de decisões e na otimização do tratamento reforça sua posição como um elemento indispensável na caixa de ferramentas dos profissionais de saúde nas UTIs do presente e do futuro

Hatching phase influences thermal preference of broilers throughout rearing.

Here we aimed for the first time to analyse whether opposite hatching patterns associated or not to high incubation temperature from day 13 to hatching interferes with the thermal preference and response of broilers to heat stress throughout the rearing period. Fertile eggs from 56-week-old broiler breeders (Cobb-500®) were used in a completely randomized trial with a 2x2 factorial arrangement (Short-Long and Long-Short hatching patterns: short time interval between internal and external pipping followed by long time interval between external pipping and hatching, and long time interval between internal and external pipping followed by short time interval between external pipping and hatching, respectively; and control and high incubation temperatures: 37.5°C and 39°C from the 13rd day, respectively). Thermal manipulation from day 13 was chosen because it is known endocrine axes are already established at this time. At hatching, male chicks were reared in climatical chamber with 16 boxes, maintained at the temperature recommended for this strain, with 4 replicates of 18 chicks per treatment. Broilers with Long-Short hatching pattern and from eggs incubation at 37.5°C preferred the lowest ambient temperature at all analyzed ages, whereas broilers with Short-Long hatching pattern and from eggs incubated at 39°C preferred the highest temperatures from 21 days of age. Heat-exposed broilers showed increased respiratory frequency in all ages analyzed, which should have to contributed to maintainance of their rectal (body) temperature. The hatching patterns did not influence the feed intake, but broilers with Short-Long hatching pattern had better feed conversion, weight gain, and body weight. High incubation temperature reduced the feed consumption, as well as the weight gain and body weight by worsening the feed conversion. The results of this study reveal that hatching patterns associated or not to high incubation temperature influence the broiler thermal preference and heat response throughout the rearing period. Chicks with Long-Short and Short-Long hatching patterns should be reared separately, although this is not practical within a hatcher

META-HEURISTIC CLONAL SELECTION ALGORITHM FOR OPTIMIZATION OF FOREST PLANNING

ABSTRACT It is important to evaluate the application of new technologies in the field of computational science to forest science. The goal of this study was to test a different kind of metaheuristic, namely Clonal Selection Algorithm, in a forest planning problem. In this problem, the total management area is 4.210 ha that is distributed in 120 stands in ages between 1 and 6 years and site indexes of 22 m to 31 m. The problem was modeled considering the maximization of the net present value subject to the constraints: annual harvested volume between 140,000 m3 and 160,000 m3, harvest ages equal to 5, 6 or 7 years, and the impossibility of division of the management unity at harvest time. Different settings for Clonal Selection Algorithm were evaluated to include: varying selection, cloning, hypermutation, and replacement rates beyond the size of the initial population. A generation value equal to 100 was considered as a stopping criteria and 30 repetitions were performed for each setting. The results were compared to those obtained from integer linear programming and linear programming. The integer linear programming, considered to be the best solution, was obtained after 1 hour of processing. The best setting for Clonal Selection Algorithm was 80 individuals in the initial population and selection. Cloning, hypermutation, and replacement rates equal to 0.20, 0.80, 0.20 and 0.50, respectively, were found. The results obtained by Clonal Selection Algorithm were 1.69% better than the integer linear programming and 4.35% worse than the linear programming. It is possible to conclude that the presented metaheuristic can be used in the resolution of forest scheduling problems

Metabolizable energy and nutrient digestibility of detoxified castor meal and castor cake for poultry

This experiment aimed to determine the metabolizable energy and the digestibility of detoxified castor meal and castor cake nutrients for broilers. A total of 180 Cobb-500TM broilers from 21 to 32 days of age was randomly distributed in three treatments with six replicates of 10 broilers - 5 males and 5 females. The experimental treatments were a reference-diet and two test-diets composed of 80% of reference-diet and 20% of detoxified castor cake or castor meal. The adaptation period ranged from 21 to 27 days of age and the total excreta collection period ranged from 28 to 32 days of age. The detoxified castor meal presented apparent metabolizable energy of 2,032 kcal/kg of natural matter, apparent metabolizable energy corrected for nitrogen balance of 1,829 kcal/kg of natural matter and the digestibility coefficients of 57.8% dry matter, 68.4% crude protein, 10.5% crude fiber, 35.9% nitrogen free extract, 80.0% ether extract and 26.6% ash. In the detoxified castor cake, the apparent metabolizable energy content was 2,550 kcal/kg of natural matter, the nitrogen corrected apparent metabolizable energy balance was of 2,320 kcal/kg of natural matter and the digestibility coefficients of 60.6% for dry matter, 77.2% for crude protein, 14.0% for crude fiber, 45.7% for nitrogen free extract, 85.4% for ether extract and 28.2% for ash. The metabolizable energy and digestibility of nutrients in detoxified castor cake are higher than in the detoxified castor meal

Torta de mamona destoxificada para alimentação de poedeiras comerciais

Objetivou-se com este experimento avaliar o efeito da inclusão de torta de mamona destoxificada (TMD) na ração de poedeiras comerciais sobre o desempenho e a qualidade interna e externa dos ovos. Foram utilizadas 200 poedeiras comerciais de 40 a 50 semanas de idade, da linhagem Hy-Line W-36®, com 1543±34g de peso corporal, que foram distribuídas em um delineamento inteiramente casualizado com cinco tratamentos (0, 5, 10, 15 e 20% de torta de mamona destoxificada na ração) com cinco repetições de oito aves cada. Foram avaliados o consumo de ração, a produção, o peso e a massa de ovos e a conversão alimentar. A qualidade interna foi avaliada por meio da unidade de Haugh, percentual de albúmen e de gema. A qualidade da casca foi medida pela espessura, densidade específica e percentual de casca. As variáveis de desempenho foram afetadas pela inclusão da TMD com resposta linear negativa para o consumo de ração e quadrática para produção de ovos, peso do ovo, massa do ovo e conversão alimentar, com os melhores níveis de 10,5, 5,7, 9,2 e 10,3% respectivamente. Não houve efeito significativo da inclusão da TMD para as variáveis de qualidade interna e externa dos ovos. Concluiu-se que a TMD pode ser incluída na ração de poedeiras em até 5,7% para otimizar o desempenho e não alterar a qualidade interna e externa dos ovos

Resumos concluídos - Fisioterapia

Resumos concluídos - Fisioterapi

Resumos concluídos - Fisioterapia

Resumos concluídos - Fisioterapi