Isolasi dan Identifikasi Senyawa Utama Fraksi Aktif Sitotoksis dari Batang Jambu Kaliang (Eugene Cumini Druce)

Telah dilakukan isolasi dan identifikasi komponen utama dari fraksi aktif sitotoksik kulit batang jambu kaliang (Eugene cumini Druce) dengan metode maserasi menggunakan pelarut methanol. Ekstrak methanol difraksinasi dengan pelarut n-heksan, diklorometan dan diuji aktitas sititoksiknya dengan metode uji kematian larva udang dalam air garam. Fraksi n-heksan menunjukkan aktifitas sitotoksik tertinggi dengan LC50 35 ppm. Dari fraksi n-heksan berhasil diisolasi kristal jarum berwarna putih dengan titik leleh 112 - 114°C sebanyak 206,5 mg dan positif steroid. Spektrum ultravioled senyawa hasil isolasi memberikan serapan maksimum pada λmaks 205,2 nm yang menunjukkan adanya transisi π à π\u27 untuk ikatan rangkap terisolasi. Spektrum infra merah senyawa hasil isolasi memberikan puncak serapan pada bilangan gelombang 3412,8 cm-1, 1049,4 cm-1, 2919,2-2848,7 cm-1, 1748,7 cm-1, 1466,4 cm-1 dan 1378,4 cm-1, yang masing-masing menunjukkan adanya gugus O-H, C-O alkohol, C-H alifatik, C=C terisolasi, C-H siklopentana dan C-H isopropyl. Spektrum masa senyawa hasil isolasi memberikan puncak dasar m/z 213 dan puncak ion molekul pada m/z 414. Berdasarkan data yang ada diduga bahwa senyawa hasil isolasi adalah stigmast-7en-3-ol dengan rumus molekul C29H50O

Additional file 6: of Identifying miRNA sponge modules using biclustering and regulatory scores

Experimentally validated mRNA-related miRNA sponge interactions and miRNA-target interactions with strong evidence. After removing replicate interactions, we have collected 46 experimentally validated mRNA-related miRNA sponge interactions, and 5195 experimentally validated miRNA-target interactions with strong evidence for validation. (XLSX 152 kb

Additional file 2: of Identifying miRNA sponge modules using biclustering and regulatory scores

GO terms and related genes associated with 10 hallmarks of cancer. There are 40 unique GO terms associated with 10 hallmarks of cancer. Only 5 cancer hallmarks (Self Sufficiency in Growth Signals, Insensitivity to Antigrowth Signals, Evading Apoptosis, Tissue Invasion and Metastasis, and Genome Instability and Mutation) have related gene sets in more than half associated GO terms. (XLSX 72 kb

Economic Assessment for a Building Thermal Insulation

Import 05/08/2014Bakalářská práce je zaměřena na ekonomické zhodnocení zateplení vybraného objektu – bytového domu v Bílině. Cílem investiční akce bylo snížit energetickou náročnost budovy a tím náklady na vytápění. Jsou popsány výhody zateplení s důsledky na kvalitu bydlení. Použitím metod hodnocení efektivnosti investic je celá akce posouzena.The thesis is focused on the economic evaluation of insulation of the selected object – the block if flats in Bílina. The aim of this investment was to reduce the energy consumption and the heating costs. It describes the benefits of insulation affecting the quality of housing. This action is assessed by using the evaluation methods of the investment.Prezenční545 - Institut ekonomiky a systémů řízenívýborn

Selenium Removal from Power Plant Waste Water with Solid Phase Extraction Materials

abstract: As selenium is toxic at low levels, treatment methods to remove selenium from industrial waste waters are needed. In this work, three groups of sorbent materials were investigated in detail for their effectiveness for selenium and arsenic removal from water: 1) nanostructured carbon-based materials, 2) layered double hydroxide (LDH)-based materials, and 3) biopolymer-based sorbents. The materials were investigated in spiked de-ionized water and waters collected from different locations at Salt River Project’s (SRP) Santan Generating Station in Gilbert, AZ. The results show that nanostructured carbon-based materials removed ~80% and up to 100% selenium and arsenic, respectively in spiked DI water. Heat treated layered double hydroxides removed close to 100% removal in selenium and arsenic spiked DI water. Isotherms conducted in spiked DI water fit the Langmuir model and showed a maximum selenate adsorption capacity of 67 mg/g for the calcined LDH powder. Results from SRP waters showed that certain LDH sorbents were effective for removing the selenium, but that higher pH and existence of competing ions affected the removal efficiencies. The functionalized biopolymer sorbent from Crystal Clear Technologies: CCT-149/OCI-B showed good removal efficiencies for both selenate and selenite in DI water. Isotherms conducted in spiked DI water for CCT-149 fit the Langmuir model and showed a maximum selenate adsorption capacity of 90.9 mg/g. Column tests using spiked DI water and waters obtained from SRP wells were investigated using both LDH and CCT-149/OCI-B. Removal of sulfate using chemical pre-treatment of the water with barium chloride resulted in about three times higher selenate loading onto the granular LDH and doubled the water volume that can be treated using CCT-149/OCI-B. The results from the column tests are being used to guide the pilot testing investigating the implementation of LDH sorbents at pilot scale at the Santan plant. The good results in the cooling tower #5 blowdown water and combined discharge waste water of SRP provide valuable information about the efficacy and efficiency of adsorptive media for the removal of selenium. Composites comprising LDH nanosheets with different substrates were successfully synthesized that were able to retain the performance in removing selenate of nanosheet LDH.Dissertation/ThesisDoctoral Dissertation Materials Science and Engineering 201

Additional file 4: of Identifying miRNA sponge modules using biclustering and regulatory scores

Differentially expressed miRNAs and mRNAs in BRCA dataset. The p-values are adjusted by Benjamini-Hochberg (BH) method. We identify 278 miRNAs (adjusted p-value <0.01), and 5602 mRNAs (adjusted p-value <1E-04) to be differentially expressed at significant level. (XLSX 1 mb

Additional file 1: of Inferring miRNA sponge co-regulation of protein-protein interactions in human breast cancer

BRCA-related miRNA sponge interaction network. The network includes 37076 miRNA sponge interactions. (XLSX 484 kb

Rankings of top ensemble methods and individual methods.

<p>Rankings of top ensemble methods and individual methods.</p

Additional file 3: of Identifying miRNA sponge modules using biclustering and regulatory scores

The list of BRCA miRNAs, BRCA genes, and cancer hallmark genes. There are 428 BRCA miRNAs, 2949 BRCA genes and 2224 cancer hallmark genes. (XLSX 77 kb

Comparison of eight individual miRNA target prediction methods.

<p>Comparison of eight individual miRNA target prediction methods.</p