Raw rehmannia radix polysaccharide can effectively release peroxidative injury induced by duck hepatitis A virus

Background: Duck viral hepatitis (DVH), caused by duck hepatitis A virus (DHAV), is a fatal contagious infectious disease which spreads rapidly with high morbidity and high mortality, and there is no effective clinical drug against DVH.Materials and Methods: Raw Rehmannia Radix Polysaccharide (RRRP), Lycii Fructus polysaccharides and Astragalus Radix polysaccharides were experimented in vitro and in vivo. Mortality rate, livers change, liver lesion scoring, peroxidative injury evaluation indexes in vitro and in vivo, and hepatic injury evaluation indexes of optimal one were detected and observed in this experiment.Results: RRRP could reduce mortality with the protection rate about 20.0% compared with that of the viral control (VC) group, finding that RRRP was the most effective against DHAV. The average liver scoring of the VC, blank control (BC), RRRP groups were 3.5, 0, 2.1. Significant difference (P<0.05) appeared between any two groups, demonstrating that it can alleviate liver pathological change. RRRP could make the hepatic injury evaluation indexes similar to BC group while the levels of the VC group were higher than other two groups in general. The levels of SOD, GSH-Px, CAT of RRRP group showed significant higher than that of VC group while the levels of NOS and MDA showed the opposite tendency, thus, RRRP could release peroxidative injury.Conclusion: RRRP was the most effective against duck hepatitis A virus (DHAV). RRRP could reduce mortality, alleviate liver pathological change, down-regulate liver lesion score, release peroxidative injury and hepatic injury. The antiviral and peroxidative injury releasing activity of RRRP for DHAV provided a platform to test novel drug strategies for hepatitis A virus in human beings.Keywords: Raw Rehmannia Radix Polysaccharide; duck hepatitis A virus; peroxidative injury; hepatic injur

RAW REHMANNIA RADIX POLYSACCHARIDE CAN EFFECTIVELY RELEASE PEROXIDATIVE INJURY INDUCED BY DUCK HEPATITIS A VIRUS

Background: Duck viral hepatitis (DVH), caused by duck hepatitis A virus (DHAV), is a fatal contagious infectious disease which spreads rapidly with high morbidity and high mortality, and there is no effective clinical drug against DVH. Materials and Methods: Raw Rehmannia Radix Polysaccharide (RRRP), Lycii Fructus polysaccharides and Astragalus Radix polysaccharides were experimented in vitro and in vivo. Mortality rate, livers change, liver lesion scoring, peroxidative injury evaluation indexes in vitro and in vivo, and hepatic injury evaluation indexes of optimal one were detected and observed in this experiment. Results: RRRP could reduce mortality with the protection rate about 20.0% compared with that of the viral control (VC) group, finding that RRRP was the most effective against DHAV. The average liver scoring of the VC, blank control (BC), RRRP groups were 3.5, 0, 2.1. Significant difference (

TCP Performance Experiment on LOBS Network Testbed

Abstract: An LOBS testbed is briefly introduced and TCP performance over OBS is experimentally investigated. Simulation and experimental results show that TCP performance is displayed differently with the loss of ACK (TCP Acknowledgement) burst and data burst

Application of Elemental Geochemistry in High-Frequency Sequence—Stratigraphic Analysis of Lacustrine Shale

The successful development of shale gas and oil in North America has created considerable interest in shale. The analysis of genetic types, the sedimentary environment, and the mudstone development mechanism within sequences is critical for evaluating shale gas and oil exploration prospects, exploration favorable zones, and resource potential. This study focused on the shale of Shahejie Formation in Dongying Depression of Bohai Bay Basin. Shale lithofacies division, geochemical analysis, and well-log analysis were performed for a sedimentary environment and its related elemental response characteristics’ identification. Based on the results, we concluded that the sedimentary environment of the lake basin evolved from the saltwater lake to the ambiguous lake and then the open lake to the delta. In response, we observed gradually decreasing Sr/Ba and Ca/Mg ratios and increasing Rb/Ca and Fe/Mn ratios during the whole process during the reduction of the salinity and the decrease in PH value and sediments’ transport distance. The relationship between ratio elements and high-frequency sequences was initially established within the shale strata. Our results show that ratios of Sr/Ba and Ca/Mg ratios near the sequence boundary are relatively low, and ratios of Fe/Mn and Rb/Ca are relatively high, while ratios of Sr/Ba and Ca/Mg near the flooding surface are relatively high, and ratios of Fe/Mn and Rb/Ca are relatively low. Those features can be used as a marker for high-frequency sequence division of shale strata. Our results provided a new theoretical basis and technical method for shale gas and oil exploration and development

Effect of Organic Viscosity-Reducing Warm-Mix Agent on the Performance of Rubber Asphalt

Dynamic mechanical analysis (DMA) and other evaluation methods were used to investigate the effect of WS-1, an organic viscosity-reducing temperature mixing agent, on the rheological and conventional properties of warm-mix rubber asphalt (WMRA). The results demonstrated that the WS-1 warm-mix agent exhibited an excellent viscosity-reducing effect and that, with the increasing of WS-1 content, the high-temperature viscosity of the WMRA decreased significantly. The viscosity and softening point of the WMRA increased at 60 °C simultaneously, with the softening point increasing by about 15 °C. The penetration and ductility decreased by about 1 mm and 6 cm, respectively, and the activation energy (Eη) and temperature sensitivity increased. These results indicated that WS-1 could improve the high-temperature performance of WMRA but had an adverse effect on its low-temperature performance. Upon using temperature scanning for the WMRA, the addition of WS-1 significantly increased the rutting factor (G*/sin δ) of the WMRA and greatly improved its rutting resistance within the temperature range examined. The addition of WS-1 changed the viscosity of WMRA, thus affecting the hot-storage stability of WMRA at high temperatures

Identification of Medicinal Compounds of Fagopyri Dibotryis Rhizome from Different Origins and Its Varieties Using UPLC-MS/MS-Based Metabolomics

Fagopyrum dibotrys, being native to southwest China, is widely distributed in Yunnan, Guizhou Provinces and Chongqing City. However, the quality of medicinal materials growing in different origins varies greatly, and cannot meet the market demand for high-quality F. dibotrys. In this study, 648 metabolites were identified, and phenolic compounds of F. dibotrys from different origins were clearly separated by principal component analysis (PCA). Our results suggested that the medicinal differences of F. dibotrys from different origins can be elucidated via the variations in the abundance of the phenolic and flavonoid compounds. We found that the epicatechin, total flavonoids and total tannin content in Yunnan Qujing (YQ) and Yunnan Kunming (YK) were higher than those in Chongqing Shizhu (CS), Chongqing Fuling (CF) and Guizhou Bijie (GB), suggesting that Yunnan Province can be considered as one of the areas that produce high-quality medicinal materials. Additionally, 1,6-di-O-galloyl-β-D-glucose, 2,3-di-O-galloyl-D-glucose and gallic acid could be used as ideal marker compounds for the quality control of F. dibotrys from different origins caused by metabolites, and the F. dibotrys planted in Yunnan Province is well worth exploiting

Heterogeneous Polymeric Matrix Induced Room-temperature Phosphorescence

Room-temperature phosphorescence (RTP) materials based on phosphor-doped polymers have been studied for more than sixty years. In these polymer-based RTP materials, the intrinsic structures and properties of polymeric matrixes are critical to their triplet emission. However, RTP materials with non-homogeneous matrixes are rarely studied, and far too little attention has been paid to the possible interferences of structures and components of polymeric matrixes on the final photophysical properties. In this work, we reported RTP materials with phase-separated matrixes consisting of two incompatible polymers, i.e., polystyrene (PS) and polyisoprene (PI). By doping difluoroboron β-diketonate derivatives into the blends or block copolymers of PS and PI, bright and long-lived green RTP emissions can be obtained under ambient conditions, while no afterglow can be observed in pure PS or PI homopolymers. The mechanism of triplet emission in heterogeneous matrixes was investigated. The findings of this work provide a new approach to the low-cost fabrication of RTP materials with comprehensive performances through high-throughput processing methods

A flavone-polysaccharide based prescription attenuates the mitochondrial dysfunction induced by duck hepatitis A virus type 1.

The principal target organ of duck hepatitis A virus type 1 (DHAV-1) is duckling liver, which is an energy-intensive organ and plays important roles in body's energy metabolism and conversion. As the "power house" of the hepatocytes, mitochondria provide more than 90% of the energy. However, mitochondria are much vulnerable to the oxidative stress for their rich in polyunsaturated fatty acids. Although previous researches have demonstrated that DHAV-1 could induce the oxidative stress in the serum of the infected ducklings, no related study on the mitochondria during the pathological process of DVH has been reported by far. To address this issue, we examined the HE stained tissue pathological slices, detected the hepatic SOD, CAT and GPX activities and MDA contents and analyzed the ATP content, mitochondrial ultrastructure and the mitochondrial SOD, GPX activities and MDA content in the liver tissues. The results showed that the hepatic redox status was significantly disturbed so that causing the mitochondrial dysfunction, ATP depletion and mitochondrial oxidative stress during the process of the DHAV-1 infection, and a prescription formulated with Hypericum japonicum flavone, Radix Rehmanniae Recens polysaccharide and Salvia plebeia flavone (HRS), which had been demonstrated with good anti-oxidative activity in serum, could effectively alleviate the hepatic injury and the oxidative stress in liver tissue induced by DHAV-1 thus alleviating the mitochondrial injury and oxidative stress. In a word, this research discovers the oxidative stress induced mitochondrial dysfunction and oxidative stress during the DVH pathological process and demonstrates HRS exerts good anti-oxidative activity in liver tissue to protect mitochondria against reactive oxygen species (ROS)

Changes of ATP content in liver tissue homogenate at 4 hpi, 8 hpi and 54 hpi (hpi: Hours Post-Injection).

<p>Liver tissue samples were obtained from individual ducklings of each group at the indicated time points, and the ATP content in liver tissue was detected with ATP assay kit. a-c Bars in the same index at the same time point without the same superscripts differ significantly (<i>p</i> < 0.05).</p