388 research outputs found
Conservation Tillage Influence on Topsoil Aggregation and Carbon Content on the Loess Plateau, China
DNMT3a in the hippocampal CA1 is crucial in the acquisition of morphine selfâadministration in rats
Drugâreinforced excessive operant responding is one fundamental feature of long-lasting addictionâlike behaviors and relapse in animals. However, the transcriptional regulatory mechanisms responsible for the persistent drugâspecific (not natural rewards) operant behavior are not entirely clear. In this study, we demonstrate a key role for one of the de novo DNA methyltransferase, DNMT3a, in the acquisition of morphine selfâadministration (SA) in rats. The expression of DNMT3a in the hippocampal CA1 region but not in the nucleus accumbens shell was significantly upâregulated after 1â and 7âday morphine SA (0.3 mg/kg/infusion) but not after the yoked morphine injection. On the other hand, saccharin SA did not affect the expression of DNMT3a or DNMT3b. DNMT inhibitor 5âazaâ2âdeoxycytidine (5âaza) microinjected into the hippocampal CA1 significantly attenuated the acquisition of morphine SA. Knockdown of DNMT3a also impaired the ability to acquire the morphine SA. Overall, these findings suggest that DNMT3a in the hippocampus plays an important role in the acquisition of morphine SA and may be a valid target to prevent the development of morphine addiction.
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Association of elevated transcript levels of interferon-inducible chemokines with disease activity and organ damage in systemic lupus erythematosus patients
JMJD3 promotes survival of diffuse large B-cell lymphoma subtypes via distinct mechanisms.
JMJD3 (Jumonji domain containing-3), a histone H3 Lys27 (H3K27) demethylase, has been reported to be involved in the antigen-driven differentiation of germinal center B-cells. However, insight into the mechanism of JMJD3 in DLBCL (Diffuse large B-cell lymphoma) progression remains poorly understood. In this study, we investigated the subtype-specific JMJD3-dependent survival effects in DLBCL. Our data showed that in the ABC subtype, silencing-down of JMJD3 inhibited interferon regulatory factor 4 (IRF4) expression in a demethylase activity-dependent fashion. IRF4 reciprocally stimulated expression of JMJD3, forming a positive feedback loop that promoted survival in these cells. Accordingly, IRF4 expression was sufficient to rescue the pro-apoptotic effect of JMJD3 suppression in the ABC, but not in the GCB subtype. In contrast, ectopic overexpression of BCL-2 completely offset JMJD3-mediated survival in the GCB DLBCL cells. In vivo, treatment with siRNA to JMJD3 reduced tumor volume concordant with increased apoptosis in either subtype. This suggests it is a common target, though the distinctive signaling axes regulating DCBCL survival offer different strategic options for treating DLBCL subtypes
Optical effects of spin currents in semiconductors
A spin current has novel linear and second-order nonlinear optical effects
due to its symmetry properties. With the symmetry analysis and the eight-band
microscopic calculation we have systematically investigated the interaction
between a spin current and a polarized light beam (or the "photon spin
current") in direct-gap semiconductors. This interaction is rooted in the
intrinsic spin-orbit coupling in valence bands and does not rely on the Rashba
or Dresselhaus effect. The light-spin current interaction results in an optical
birefringence effect of the spin current. The symmetry analysis indicates that
in a semiconductor with inversion symmetry, the linear birefringence effect
vanishes and only the circular birefringence effect exists. The circular
birefringence effect is similar to the Faraday rotation in magneto-optics but
involves no net magnetization nor breaking the time-reversal symmetry.
Moreover, a spin current can induce the second-order nonlinear optical
processes due to the inversion-symmetry breaking. These findings form a basis
of measuring a pure spin current where and when it flows with the standard
optical spectroscopy, which may provide a toolbox to explore a wealth of
physics connecting the spintronics and photonics.Comment: 16 pages, 7 fig
Detection of Viable and Total Bacterial Community in the Pit Mud of Chinese Strong-Flavor Liquor Using Propidium Monoazide Combined With Quantitative PCR and 16S rRNA Gene Sequencing
MAX-DOAS measurements of tropospheric NO2 and HCHO in Nanjing and a comparison to ozone monitoring instrument observations
In this paper, we present long-term observations of atmospheric nitrogen dioxide (NO2) and formaldehyde (HCHO) in Nanjing using a Multi-AXis Differential Optical Absorption Spectroscopy (MAX-DOAS) instrument. Ground-based MAX-DOAS measurements were performed from April 2013 to February 2017. The MAX-DOAS measurements of NO2 and HCHO vertical column densities (VCDs) are used to validate ozone monitoring instrument (OMI) satellite observations over Nanjing. The comparison shows that the OMI observations of NO2 correlate well with the MAX-DOAS data with Pearson correlation coefficient (R) of 0.91. However, OMI observations are on average a factor of 3 lower than the MAX-DOAS measurements. Replacing the a priori NO2 profiles by the MAX-DOAS profiles in the OMI NO2 VCD retrieval would increase the OMI NO2 VCDs by similar to 30% with correlation nearly unchanged. The comparison result of MAX-DOAS and OMI observations of HCHO VCD shows a good agreement with R of 0.75 and the slope of the regression line is 0.99. An age-weighted backward-propagation approach is applied to the MAX-DOAS measurements of NO2 and HCHO to reconstruct the spatial distribution of NO2 and HCHO over the Yangtze River Delta during summer and winter time. The reconstructed NO2 fields show a distinct agreement with OMI satellite observations. However, due to the short atmospheric lifetime of HCHO, the backward-propagated HCHO data do not show a strong spatial correlation with the OMI HCHO observations. The result shows that the MAX-DOAS measurements are sensitive to the air pollution transportation in the Yangtze River Delta, indicating the air quality in Nanjing is significantly influenced by regional transportation of air pollutants. The MAX-DOAS data are also used to evaluate the effectiveness of air pollution control measures implemented during the Youth Olympic Games 2014. The MAX-DOAS data show a significant reduction of ambient aerosol, NO2 and HCHO (30 %-50 %) during the Youth Olympic Games. Our results provide a better understanding of the transportation and sources of pollutants over the Yangtze River Delta as well as the effect of emission control measures during large international events, which are important for the future design of air pollution control policies
T-614, a novel immunomodulator, attenuates joint inflammation and articular damage in collagen-induced arthritis
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