Pea albumin extracted from pea (Pisum sativum L.) seed protects mice from high fat diet-induced obesity by modulating lipid metabolism and gut microbiota

Plant protein has been reported to play a key role in the prevention of obesity and associated complications. It is unknown whether pea albumin may exert anit-obesity and obesity-associated metabolic disorders alleviation. Pea albumin was isolated from pea seed (Pisum sativum L.) and determined its functional role for anti-obesity and metabolic disorders alleviation in high fat diet (HFD)-fed mice. Pea albumin administration reduced body weight gain, improved glucose tolerance and insulin sensitivity, reduced inflammatory cytokines secretion, and alleviated hepatic steatosis in HFD-fed mice. Interestingly, pea albumin inhibited lipid accumulation in 3T3-L1 cells in vitro and modulated lipid metabolism by upregulating critical proteins implicated in lipolysis and fatty acid oxidation, and downregulating lipogenesis in vivo. Moreover, pea albumin restored gut microbial composition to normal fat diet condition and selectively promoted the growth of beneficial gut bacteria (Akkermansia, Parabacteroides etc.). Collectively, the data demonstrated that pea albumin protects mice from HFD-induced obesity and associated metabolic disorders

Cocaine Preferentially Potentiates Fast Releasable Vesicle Pool in Mouse Dopaminergic Striatum In Vivo

BiophysicsSCI(E)CPCI-S(ISTP)0MEETING ABSTRACT2498A-499A10

Engineering 3D bicontinuous hierarchically macro-mesoporous LiFePO₄/C nanocomposite for lithium storage with high rate capability and long cycle stability

A highly crystalline three dimensional (3D) bicontinuous hierarchically macro-mesoporous LiFePO(4)/C nanocomposite constructed by nanoparticles in the range of 50~100 nm via a rapid microwave assisted solvothermal process followed by carbon coating have been synthesized as cathode material for high performance lithium-ion batteries. The abundant 3D macropores allow better penetration of electrolyte to promote Li(+) diffusion, the mesopores provide more electrochemical reaction sites and the carbon layers outside LiFePO(4) nanoparticles increase the electrical conductivity, thus ultimately facilitating reverse reaction of Fe(3+) to Fe(2+) and alleviating electrode polarization. In addition, the particle size in nanoscale can provide short diffusion lengths for the Li(+) intercalation-deintercalation. As a result, the 3D macro-mesoporous nanosized LiFePO(4)/C electrode exhibits excellent rate capability (129.1 mA h/g at 2 C; 110.9 mA h/g at 10 C) and cycling stability (87.2% capacity retention at 2 C after 1000 cycles, 76.3% at 5 C after 500 cycles and 87.8% at 10 C after 500 cycles, respectively), which are much better than many reported LiFePO(4)/C structures. Our demonstration here offers the opportunity to develop nanoscaled hierarchically porous LiFePO(4)/C structures for high performance lithium-ion batteries through microwave assisted solvothermal method

Transient Receptor Potential V Channels Are Essential for Glucose Sensing by Aldolase and AMPK

Fructose-1,6-bisphosphate (FBP) aldolase links sensing of declining glucose availability to AMPK activation via the lysosomal pathway. However, how aldolase transmits lack of occupancy by FBP to AMPK activation remains unclear. Here, we show that FBP-unoccupied aldolase interacts with and inhibits endoplasmic reticulum (ER)-localized transient receptor potential channel subfamily V, inhibiting calcium release in low glucose. The decrease of calcium at contact sites between ER and lysosome renders the inhibited TRPV accessible to bind the lysosomal v-ATPase that then recruits AXIN:LKB1 to activate AMPK independently of AMP. Genetic depletion of TRPVs blocks glucose starvation-induced AMPK activation in cells and liver of mice, and in nematodes, indicative of physical requirement of TRPVs. Pharmacological inhibition of TRPVs activates AMPK and elevates NAD(+) levels in aged muscles, rejuvenating the animals' running capacity. Our study elucidates that TRPVs relay the FBP-free status of aldolase to the reconfiguration of v-ATPase, leading to AMPK activation in low glucose

Метод расчета зависимости динамики доходов работников от уровня образования в Республике Беларусь

We report a study of the process e(+)e(-) -&gt; (D*(D) over bar*)(0)pi(0) using e(+)e(-) collision data samples with integrated luminosities of 1092 pb(-1) at root s = 4.23 GeV and 826 pb(-1) at root s = 4.26 GeV collected with the BESIII detector at the BEPCII storage ring. We observe a new neutral structure near the (D*(D) over bar*)(0) mass threshold in the pi(0) recoil mass spectrum, which we denote as Z(c)(4025)(0). Assuming a Breit-Wigner line shape, its pole mass and pole width are determined to be (4025.5(-4.7)(+2.0) +/- 3.1) MeV/c(2) and (23.0 +/- 6.0 +/- 1.0) MeV, respectively. The Born cross sections of e(+)e(-) -&gt; Z(c)(4025)(0)pi(0) -&gt; (D*(D) over bar*)(0)pi(0) are measured to be (61.6 +/- 8.2 +/- 9.0) pb at root s = 4.23 GeV and (43.4 +/- 8.0 +/- 5.4) pb at root s = 4.26 GeV. The first uncertainties are statistical and the second are systematic.Funding: The BESIII Collaboration thanks the staff of BEPCII and the IHEP computing center for their strong support. This work is supported in part by the National Key Basic Research Program of China under Contract No. 2015CB856700; the National Natural Science Foundation of China (NSFC) under Contracts No. 11125525, No. 11235011, No. 11275266, No. 11322544, No. 11335008, and No. 11425524; the Chinese Academy of Sciences (CAS) Large-Scale Scientific Facility Program; the CAS Center for Excellence in Particle Physics (CCEPP); the Collaborative Innovation Center for Particles and Interactions (CICPI); the Joint Large-Scale Scientific Facility Funds of the NSFC and the CAS under Contracts No. 11179007, No. U1232201, and No. U1332201; the CAS under Contracts No. KJCX2-YW-N29 and No. KJCX2-YW-N45; the 100 Talents Program of the CAS; INPAC and the Shanghai Key Laboratory for Particle Physics and Cosmology; German Research Foundation DFG under Contract No. Collaborative Research Center CRC-1044; the Istituto Nazionale di Fisica Nucleare, Italy; the Ministry of Development of Turkey under Contract No. DPT2006K-120470; the Russian Foundation for Basic Research under Contract No. 14-07-91152; the U.S. Department of Energy under Contracts No. DE-FG02-04ER41291, No. E-FG02-05ER41374, No. DE-FG02-94ER40823, and No. DESC0010118; the U.S. National Science Foundation; the University of Groningen (RuG) and the Helmholtzzentrum fuer Schwerionenforschung GmbH (GSI), Darmstadt; and the WCU Program of National Research Foundation of Korea under Contract No. R32-2008-000-10155-0.</p

Insights into APC/C: from cellular function to diseases and therapeutics

Anaphase-promoting complex/cyclosome (APC/C) is a multifunctional ubiquitin-protein ligase that targets different substrates for ubiquitylation and therefore regulates a variety of cellular processes such as cell division, differentiation, genome stability, energy metabolism, cell death, autophagy as well as carcinogenesis. Activity of APC/C is principally governed by two WD-40 domain proteins, Cdc20 and Cdh1, in and beyond cell cycle. In the past decade, the results based on numerous biochemical, 3D structural, mouse genetic and small molecule inhibitor studies have largely attracted our attention into the emerging role of APC/C and its regulation in biological function, human diseases and potential therapeutics. This review will aim to summarize some recently reported insights into APC/C in regulating cellular function, connection of its dysfunction with human diseases and its implication of therapeutics

Experimental study on immersion collapsibility process and vertical stress characteristics of large thickness loess foundation in Jingyuan area

In order to study the water infiltration and self-weight collapse deformation characteristics of Jingyuan loess with large thickness under the condition of immersion, a field immersion test without water injection holes was carried out in the self-weight collapsed loess site of Jingyuan North Station along the Zhongwei−Lanzhou Railway. The surface and underground collapsible deformation, cracks, water content and vertical stress in the soil around the test pit were monitored and analyzed. The water diffusion, self-weight collapsible characteristics and vertical stress in the soil were studied, and the regional correction coefficient β0 value and wetting angle were discussed. The results showed that: the change of volumetric water content was divided into four stages: immersion stabilization (two), rapid increase (one) and slow increase (one). In the immersion process, the vertical infiltration of water was accelerated and the radial diffusion was slowed down at 21 m, and the final shape of the wetting front was presented as elliptical. According to the water content test results of exploratory wells and boreholes, the maximum wetting angle was calculated to be 41°. The self-weight collapse process of loess in the site went through three stages: severe collapse, slow collapse and consolidation stabilization. At the end of the test, a total of 13 ring cracks were developed, and the farthest point of the cracks was 26 m from the edge of the test pit. According to the laboratory test and field test results, it was suggested that the regional correction coefficient should be corrected along the depth of the soil layer, and the β0 value was taken as 1.05 within 0−10 m and 0.95 within 10−27 m. In the depth range from the surface to 21 m, the foundation soil was saturated and fully collapsed. The vertical stress in the soil increased linearly along the depth, and the vertical stress in the soil was close to the saturated self-weight stress. The foundation soil below 21 m failed to collapse entirely, and the vertical stress in the soil decreased gradually. The research results could be applied to the later construction of Zhongwei−Lanzhou Railway and provide a reference for other regional engineering projects