Testicular development of Zebu bulls after chronic treatment with a gonadotropin-releasing hormone agonist

The objective was to compare testis characteristics of Zebu bulls treated with the GnRH agonist, deslorelin, at different times and for different durations during their development. An additional objective was to determine the usefulness of a stain for the transcription factor GATA-binding protein 4 (GATA-4) as a specific marker for Sertoli cell nuclei in cattle. Bulls (54) were allocated to nine groups (n = 6) and received s.c. deslorelin implants as follows: G1 = from birth to 3 mo of age; G2 = from 3 to 6 mo; G3 = from 6 to 9 mo; G4 = from 9 to 12 mo; G5 = from birth to 15 mo; G6 = from 3 to 15 mo; G7 = from 6 to 15 mo; G8 = from 12 to 15 mo; and G9 (control) = no implant. Bulls were castrated at 19 mo of age. Paraffin sections (10 mu m) were subjected to quantitative morphometry and GATA-4 immunohistochemistry. At castration, all bulls in the control group (6/6) had attained puberty (scrotal circumference ! 28 cm), whereas a smaller proportion (P < 0.05) had reached puberty in G2 (2/5) and G6 (1/ 6). Bulls in G2 and G6 also had a lesser (P < 0.05) testis weight compared with the control group. Total volume of seminiferous epithelium and total daily sperm production in G2 and G6 were only half that observed in the control group. Spermatids were observed in less than 50% of seminiferous tubules in G2, G6, and G7 compared with 82% in the control group (P < 0.05). Staining for GATA-4 was specific for and abundant in the Sertoli cell nucleus in both pre- and postpubertal bulls, and no other cell nucleus inside the seminiferous tubule was positive for GATA-4. Total number of Sertoli cells was not affected by treatment (P = 0.45), but nuclear volume was smaller in G2 and G6 (P < 0.05) compared with the control group. In conclusion, treatment of Zebu bulls with deslorelin had no apparent beneficial effect on testis development and delayed puberty when treatment was initiated at 3 mo of age. Staining for GATA-4 was a useful method for identifying and quantifying Sertoli cell nuclei in both pre- and postpubertal bulls

Effect of chronic treatment with the gonadotrophin-releasing hormone agonist azagly-nafarelin on basal concentrations of LH in prepubertal bulls

Administration of GnRH agonist for an extended period inhibits pulsatile LH release but enhances testicular function of bulls. The mechanism whereby long-term administration of GnRH agonist enhances testosterone concentration in the blood of bulls has not been determined. The aim of this study was to determine whether chronic treatment with the GnRH agonist, azagly-nafarelin, increases blood concentrations of LH and FSH in prepubertal bulls. Two different doses of the GnRH agonist were administered via Alzet mini-osmotic pumps for 28 days. Blood samples were collected at 20 min intervals for 24 h at days 2, 13 and 25 of treatment. Agonist-treated groups had reduced testosterone pulse frequency (P < 0.05) and increased mean and basal concentrations of testosterone (P < 0.05) compared with untreated control bulls. Basal LH concentrations were higher in agonist-treated bulls during all three periods (P < 0.05) and overall 0 ng ml(-1) higher, compared with control bulls; (P < 0.001). Frequency of LH pulses in the agonist-treated groups was reduced to less than one pulse in 24 h. Agonist-treated bulls tended to have (P < 0.10) or had (P < 0.05) a slight but significant increase in blood FSH concentration. In conclusion, the higher blood testosterone concentration in bulls after prolonged treatment with GnRH agonist may result, at least in part, from changes in the testes induced by enhanced basal concentration of LH

Potencial de descompactação de um Argissolo promovido pelo capim-tifton 85 Loosening potential of an Argisol promoted by tifton 85 grass

A produção de forragens conservadas utilizando-se um tráfego intenso de maquinário sob condições inadequadas de água no solo, tem condicionado compactação do solo e a degradação das pastagens. No enfrentamento desta grave questão faz-se necessário o conhecimento do potencial de descompactação do solo, promovido por algumas forrageiras. Objetivou-se, com o presente trabalho, avaliar a descompactação de um Argissolo ao ser cultivado com capim-tifton 85 e sua produção. O experimento foi conduzido na Universidade Federal de Lavras, no período de fevereiro a junho de 2007, em delineamento inteiramente casualizado cultivando-se o capim-tifton nos seguintes graus de compactação do solo: 66, 83, 88, 92 e 98%. Avaliaram-se os atributos físicos, o comportamento compressivo do solo e a produção de massa seca do capim. A descompactação foi quantificada pela redução da pressão de preconsolidação após o cultivo. Os modelos de capacidade de suporte de carga refletiram as alterações na macroporosidade do solo. Nas condições do estudo o capim-tifton 85 promoveu a descompactação do solo, a qual é diretamente proporcional ao grau de compactação inicial. A produção de forragem aumenta sempre que o solo é descompactado.<br>The production of conserved forages using an intense traffic of machinery under inadequate conditions of water content in the soil has caused soil compaction and the degradation of pastures. In order to face this serious problem it is necessary to know the potential of soil loosening by some forage plants. The objective of the present study was to evaluate the loosening of an Argisol cultivated with Tifton 85 grass, as well as its production. The experiment was conducted at the Federal University of Lavras, from February to June 2007, using a completely randomized design, cultivating the Tifton 85 grass under the following compaction degrees: 66, 83, 88, 92 and 98%. The physical attributes, the compressive behavior of soil and the production of dry mass of grass were evaluated. The loosening was quantified by the reduction of the preconsolidation pressure after cultivation. The bearing capacity models reflected the changes in soil macroporosity. Under study conditions, the Tifton 85 grass promoted the soil loosening proportional to the initial compression degree. The production of forage increased as the soil is loosened