New insights on repellent recognition by <i>Anopheles gambiae</i> odorant-binding protein 1

It is generally recognized that insect odorant binding proteins (OBPs) mediate the solubilisation and transport of hydrophobic odorant molecules and contribute to the sensitivity of the insect olfactory system. However, the exact mechanism by which OBPs deliver odorants to olfactory receptors and their role, if any, as selectivity filters for specific odorants, are still a matter of debate. In the case of Anopheles gambiae recent studies indicate that ligand discrimination is effected through the formation of heterodimers such as AgamOBP1 and AgamOBP4 (odorant binding proteins 1 and 4 from Anopheles gambiae). Furthermore, AgamOBPs have been reported to be promiscuous in binding more than one ligand simultaneously and repellents such as DEET (N,N-diethyl-3-toluamide) and 6-MH (6-methyl-5-hepten-2-one) interact directly with mosquito OBPs and/or compete for the binding of attractive odorants thus disrupting OBP heterodimerisation. In this paper, we propose mechanisms of action of DEET and 6-MH. We also predict that ligand binding can occur in several locations of AgamOBP1 with partial occupancies and propose structural features appropriate for repellent pharmacophores

Creating globalisation: 'patriotic internationalism' and symbiotic power relations in the Post-WW2 era

This paper engages a number of important and complex questions on the contemporary Globalisation agenda. In the first instance it is concerned with the question of what globalisation actually means. It confronts this issue in both conventional terms (i.e. the current debate over ‘meaning’ which generally pits neo-liberal perspectives against a variety of critical alternatives) and in terms of a broader historical and intellectual frame of reference which, it suggests, is a more appropriate context for the debate. The suggestion, more precisely, is that the globalisation phenomenon of the current era, is best understood in terms of some fundamental organising principles drawn from a modern international political economy (IPE) agenda in which (the major) states and an advanced, expansionist brand of capitalism continue to dominate, albeit as part of a significantly reformulated symbiotic power relationship. The second and primary focus of the paper is concerned to illustrate how this traditional symbiosis actually works at the core of contemporary globalisation, in theory and practice. It does so in concentrating on that period between 1945 and the present when, it is suggested, a series of policy decisions taken by the USA at Bretton-Woods, in order to retain and enhance its post-WW2 systemic advantages, were intrinsic also to the breakdown of the Bretton-Woods system, the development of increasingly deregulated economic sector by the mid-1970s, the ‘casino capitalism’ of the 1980s and the coherent neo-liberal agenda of the 1990s which, in the post-Cold War years has invoked a new era of liberal free-market economics as the keystone of global peace and prosperity in the future. Rejecting the one-sidedness of this perspective the paper maintains that a symbiosis of the political and the economic still characterises the age of neo-liberal dominance. That, indeed, the notion of independent economic forces imposing themselves upon effectively impotent state actors misses the historical, political and ideological point about the nature of systemic agency and structure in the modern IPE. It seeks, in this regard, to illustrate how a major capitalist state, such as the USA, is intrinsically connected to the ‘economic’ success of the neo-liberal globalist agenda, and how its ostensibly independent ‘economic’ agenda is, if anything, increasingly dependent upon a Triad of major states (i.e. centred on the U.S., the E.U and Japan) for support, sustenance and profit. The brief final section of the paper touches on some of the possible implications of this scenario for the global future

Scheduling with partial orders and a causal model

In an ongoing project at Honeywell SRC, we are constructing a prototype scheduling system for a NASA domain using the 'Time Map Manager' (TMM). The TMM representations are flexible enough to permit the representation of precedence constraints, metric constraints between activities, and constraints relative to a variety of references (e.g., Mission Elapsed Time vs. Mission Day). The TMM also supports a simple form of causal reasoning (projection), dynamic database updates, and monitoring specified database properties as changes occur over time. The greatest apparent advantage to using the TMM is the flexibility added to the scheduling process: schedules are constructed by a process of 'iterative refinement,' in which scheduling decisions correspond to constraining an activity either with respect to another activity or with respect to one time line. The schedule becomes more detailed as activities and constraints are added. Undoing a scheduling decision means removing a constraint, not removing an activity from a specified place on the time line. For example, we can move an activity around on the time line by deleting constraints and adding new ones

Attitude Determination and Control System (ADCS) and Maintenance and Diagnostic System (MDS): A maintenance and diagnostic system for Space Station Freedom

The Maintenance and Diagnostic System (MDS) that is being developed at Honeywell to enhance the Fault Detection Isolation and Recovery system (FDIR) for the Attitude Determination and Control System on Space Station Freedom is described. The MDS demonstrates ways that AI-based techniques can be used to improve the maintainability and safety of the Station by helping to resolve fault anomalies that cannot be fully determined by built-in-test, by providing predictive maintenance capabilities, and by providing expert maintenance assistance. The MDS will address the problems associated with reasoning about dynamic, continuous information versus only about static data, the concerns of porting software based on AI techniques to embedded targets, and the difficulties associated with real-time response. An initial prototype was built of the MDS. The prototype executes on Sun and IBM PS/2 hardware and is implemented in the Common Lisp; further work will evaluate its functionality and develop mechanisms to port the code to Ada

Germin and germin-like proteins: evolution, structure, and function

Germin and germin-like proteins (GLPs) are encoded by a family of genes found in all plants. They are part of the cupin superfamily of biochemically diverse proteins, a superfamily that has a conserved tertiary structure, though with limited similarity in primary sequence. The subgroups of GLPs have different enzyme functions that include the two hydrogen peroxide-generating enzymes, oxalate oxidase (OxO) and superoxide dismutase. This review summarizes the sequence and structural details of GLPs and also discusses their evolutionary progression, particularly their amplification in gene number during the evolution of the land plants. In terms of function, the GLPs are known to be differentially expressed during specific periods of plant growth and development, a pattern of evolutionary subfunctionalization. They are also implicated in the response of plants to biotic (viruses, bacteria, mycorrhizae, fungi, insects, nematodes, and parasitic plants) and abiotic (salt, heat/cold, drought, nutrient, and metal) stress. Most detailed data come from studies of fungal pathogenesis in cereals. This involvement with the protection of plants from environmental stress of various types has led to numerous plant breeding studies that have found links between GLPs and QTLs for disease and stress resistance. In addition the OxO enzyme has considerable commercial significance, based principally on its use in the medical diagnosis of oxalate concentration in plasma and urine. Finally, this review provides information on the nutritional importance of these proteins in the human diet, as several members are known to be allergenic, a feature related to their thermal stability and evolutionary connection to the seed storage proteins, also members of the cupin superfamily

Electoral Uncertainty, Fiscal Policies and Growth: Theory and Evidence from Germany, the UK and the US

In this paper we study the link between elections, fiscal policy and economic growth/fluctuations. The set-up is a dynamic stochastic general equilibrium model of growth and endogenously chosen fiscal policy, in which two political parties can alternate in power. The party in office chooses jointly how much to tax and how to allocate its total expenditure between public consumption and production services. The main theoretical prediction is that forward-looking incumbents, with uncertain prospects of re-election, find it optimal to follow relatively shortsighted fiscal policies, and that this lowers economic growth. The model is estimated using quarterly data for Germany, the UK and the US from 1960 to 1999. Our econometric results provide clear support for the main theoretical prediction. They also give plausible and significant estimates for the productivity of public production services, the weight which households place on public consumption services relative to private consumption and the time discount rate. Moreover, we find that changes in electoral uncertainty produce the longest lasting fluctuations in the European economies followed by the US.