LOAD-DEFLECTION BEHAVIOR OF RATTAN CHAIR SEATS

The static and fatigue performances of seat foundations of natural rattan chairs subjected to vertical loads were investigated.  Static performance evaluation results indicate that rattan strip weaving patterns have significant effects on the vertical load carrying capacity and stiffness performance of chair seat foundations.  Herringbone and grid pattern woven seat foundations had significantly higher vertical load carrying capacity than those made with a square-corner pattern.  Square-corner pattern seat foundations yielded a softer sitting surface than herringbone and grid patterns.  Herringbone and grid pattern seat foundations can provide firmer sitting feel and good deep down support for heavier sitters.  The Burger model could be used to describe the force-deformation-time behavior of a rattan woven seat foundation subjected to vertical cyclic loading

COMPRESSIVE CREEP AND RECOVERY BEHAVIORS OF SEAT CUSHIONS IN UPHOLSTERED FURNITURE

This study investigated effects of compressive load magnitude and cover and core materials on the force-deformation-time behavior of seat cushions commonly used in upholstered furniture. Results indicated that the Burger and Kelvin models could be used to describe the creep and recovery behavior of a furniture seat cushioning system composed of foam, spring, and cover materials, respectively. Statistical analyses of experimental data indicated that the magnitude of creep loads had significant effects on the viscoelastic constants in mathematical expressions derived from the Burger model for describing the force-deformation-time behavior of the cushions evaluated. Foam cushions with coil springs had significantly greater viscoelastic constants than those without. Changing cushion cover material from leather to fabric had no significant effect on the elastic constant of tested cushion materials, but increased theviscous constant and delayed elastic-deformation-related damping constants

Tensile and Bending Moment Resistances of T-Shaped Joints in Rattan Chairs

Effects of inner fastener type, wrapping pattern and material type, and member material type on ultimate tensile and bending moment resistances of T-shaped joints in rattan chair construction were investigated based on the L9 (34) orthogonal array experimental design. The range analyses indicated that the order of impact on ultimate tensile loads of four factors was inner fastener type > wrapping pattern > member material type > wrapping material type, whereas the order of impact on ultimate bending moment was inner fastener type > wrapping material type > wrapping pattern > member material type. Analysis of variance indicated that inner fastener type affected ultimate tensile and bending moment the most among the four factors with percentages of contribution of 51.19 and 47.06 to tensile and bending moment, respectively. Optimal combinations of factors and their levels that yielded the highest ultimate tensile and bending moment resistances were identified for T-shaped, end-to-side joints in rattan materials

LATERAL LOAD RESISTANCE BEHAVIOR OF WOOD-PLASTIC-TO METAL SINGLE-BOLT CONNECTIONS IN OUTDOOR FURNITURE

The lateral load resistance behavior of an unconstrained, two-member, single-bolt connection in outdoor furniture applications was investigated. The unconstrained connection consisted of a wood-plastic composite (WPC) main member fastened to a metal plate as a side member through a 6.35-mm-diameter bolt without a nut or washer used. Experimental results indicated that unconstrained WPC-to-metal single-bolt connections had a significantly higher lateral resistance load if the WPC main member is loaded in the direction perpendicular to the WPC material extrusion direction than the parallel direction. Tested connections failed with bolts having one plastic hinge bent, which occurred at the interface between the metal plate and WPC main member, accompanied by the WPC main members having a compressive yield fracture at their sides close to the metal plate, but no obvious compressive mark was observed at the opposite sides. Proposed linear and yield mechanical models were verified experimentally as a valid means for deriving estimation equations of lateral resistance loads of unconstrained WPC-to-metal single-bolt connections

Effect of Coating Thickness on Sound Absorption Property of Four Wood Species Commonly Used for Piano Soundboards

Effects of polyurethane (PU) coating thicknesses (0.15, 0.30, 0.45, and 0.60 mm) on sound absorption coefficients of four wood species were investigated using the standing wave ratio method with an input sound vibration frequency range set between 125 and 4000 Hz. Wood species of four specific gravity (SG) levels were Korean spruce, European spruce, Sitka spruce, and Picea brachytyla. Experimental results indicated that PU coating can significantly increase sound absorption coefficients of higher SG species such as Sitka spruce and Picea brachytyla in all tested frequency levels, but this significant increase was not observed in lower SG species such as Korean and European spruces when tested in the frequency range from 800 to 2000 Hz. Effects of coating thickness on sound absorption coefficients of four evaluated species were found to interact with wood SG values and input sound vibration frequency ranges. Specifically, coating 0.30-mm-thick PU on Korean and European spruces tends to result in significantly lower sound absorption coefficients among the ones coated with four evaluated thicknesses when tested at the frequency less than 800 Hz, but PU coating thickness resulting in lower sound absorption coefficients on Sitka spruce and Picea brachytyla was 0.15 mm. Sitka spruce and Picea brachytyla coated with 0.30- and 0.6-mm-thick PU had lower sound absorption coefficients when tested at the frequency ranging from 1000 to 2000 Hz. When tested at the frequency greater than 2500 Hz, sound absorption coefficients of four coated species increased as coating thickness increased from 0.30 to 0.60 mm with an increment of 0.15 mm, but these four species coated with three thicker PU had significantly lower sound absorption coefficients than the ones coated with 0.15-mm-thick PU. The uncoated higher SG species tended to have lower sound absorption coefficients than uncoated lower SG ones when tested in the frequency ranging from 500 to 4000 Hz, but the differences were not found when tested under the frequency less than 400 Hz. Coating four species with different thicknesses of PU could alter their SG effects on their sound absorption coefficients

Microtissues Enhance Smooth Muscle Differentiation and Cell Viability of hADSCs for Three Dimensional Bioprinting

Smooth muscle differentiated human adipose derived stem cells (hADSCs) provide a crucial stem cell source for urinary tissue engineering, but the induction of hADSCs for smooth muscle differentiation still has several issues to overcome, including a relatively long induction time and equipment dependence, which limits access to abundant stem cells within a short period of time for further application. Three-dimensional (3D) bioprinting holds great promise in regenerative medicine due to its controllable construction of a designed 3D structure. When evenly mixed with bioink, stem cells can be spatially distributed within a bioprinted 3D structure, thus avoiding drawbacks such as, stem cell detachment in a conventional cell-scaffold strategy. Notwithstanding the advantages mentioned above, cell viability is often compromised during 3D bioprinting, which is often due to pressure during the bioprinting process. The objective of our study was to improve the efficiency of hADSC smooth muscle differentiation and cell viability of a 3D bioprinted structure. Here, we employed the hanging-drop method to generate hADSC microtissues in a smooth muscle inductive medium containing human transforming growth factor β1 and bioprinted the induced microtissues onto a 3D structure. After 3 days of smooth muscle induction, the expression of α-smooth muscle actin and smoothelin was higher in microtissues than in their counterpart monolayer cultured hADSCs, as confirmed by immunofluorescence and western blotting analysis. The semi-quantitative assay showed that the expression of α-smooth muscle actin (α-SMA) was 0.218 ± 0.077 in MTs and 0.082 ± 0.007 in Controls; smoothelin expression was 0.319 ± 0.02 in MTs and 0.178 ± 0.06 in Controls. Induced MTs maintained their phenotype after the bioprinting process. Live/dead and cell count kit 8 assays showed that cell viability and cell proliferation in the 3D structure printed with microtissues were higher at all time points compared to the conventional single-cell bioprinting strategy (mean cell viability was 88.16 ± 3.98 vs. 61.76 ± 15% for microtissues and single-cells, respectively). These results provide a novel way to enhance the smooth muscle differentiation of hADSCs and a simple method to maintain better cell viability in 3D bioprinting

Heterologous Boost Following Mycobacterium bovis BCG Reduces the Late Persistent, Rather Than the Early Stage of Intranasal Tuberculosis Challenge Infection

Adults are the leading population affected by tuberculosis (TB) epidemic and death. Developing an effective vaccine against adult TB is urgently needed. Mycobacterium bovis Bacillus Calmette-Guerin (BCG) prime-heterologous boost strategy has been explored extensively to protect adults against primary TB infection, but the majority of experimental regimens have not improved the protection primed by the BCG vaccine. The reason attributed to the failure remains unknown. In this study, CTT3H-based vaccines, namely DMT adjuvanted CTT3H subunit or DNA vaccine (pCTT3H-DMT), and recombinant adenovirus rAdCTT3H were constructed. Protective efficacy and immunogenicity of BCG prime-CTT3H based boosters were compared in C57BL/c mice models of primary or late persistent TB infection. Similar protective efficacy against early intranasal infection was provided by different CTT3H-based vaccines alone in vaccinated mice, and their protection was inferior to that of the BCG vaccine. In addition, CTT3H-based heterologous boosters did not enhance the protection conferred by the BCG vaccine against primary infection. However, all of these three boosters provided stronger protection against late persistent TB infection than BCG alone, regardless of vaccine types. Although BCG prime-boosters elicited Th1-biased responses to the antigen CTT3H, the number of CTT3H-sepcific IFN-γ-expressing TEM (CD62LloCD44hi) and IL-2-expressing TCM (CD62LhiCD44hi) cells in the spleen was not improved before exposure to Mycobacterium tuberculosis infection. In contrast, IFN-γ+ TEM and IL-2+ TCM cells in spleens, especially in lungs were significantly increased in BCG prime-boosters after exposure vaccination. Our results indicate that BCG prime-boost strategy might be a promising measure for the prevention against late persistent TB infection by induction of IFN-γ+ TEM and IL-2+ TCM cells in the lung, which can be used as alternative biomarkers for guiding the clinical practice and future development of TB vaccine for adults

Excitation of unidirectional exchange spin waves by a nanoscale magnetic grating

Magnon spintronics is a prosperous field that promises beyond-CMOS technology based on elementary excitations of the magnetic order that act as information carriers for future computational architectures. Unidirectional propagation of spin waves is key to the realization of magnonic logic devices. However, previous efforts to enhance the Damon-Eshbach-type nonreciprocity did not realize (let alone control) purely unidirectional propagation. Here we experimentally demonstrate excitations of unidirectional exchange spin waves by a nanoscale magnetic grating consisting of Co nanowires fabricated on an ultrathin yttrium iron garnet film. We explain and model the nearly perfect unidirectional excitation by the chirality of the magneto-dipolar interactions between the Kittel mode of the nanowires and the exchange spin waves of the film. Reversal of the magnetic configurations of film and nanowire array from parallel to antiparallel changes the direction of the excited spin waves. Our results raise the prospect of a chiral magnonic logic without the need for fragile surface states