11 research outputs found
Higgs Boson Phenomenology in a Simple Model with Vector Resonances
In this paper we consider a simple scenario where the Higgs boson and two
vector resonances are supposed to arise from a new strong interacting sector.
We use the ATLAS measurements of the dijet spectrum to set limits on the masses
of the resonances. Additionally we compute the Higgs boson decay to two photons
and found, when compare to the Standard Model prediction, a small excess which
is compatible with ATLAS measurements. Finally we make prediction for
Higgs-strahlung processes for the LHC running at 14 TeV
SNP genotypes from Magallanes
<p>Hybrid zones among mussel species have been extensively studied in the northern hemisphere. In South America, it has only recently become possible to study the natural hybrid zones, due to the clarification of the taxonomy of native mussels of the <em>Mytilus</em> genus. Analyzing 54 SNP markers, we show the genetic species composition and admixture in the hybrid zone between <em>M. chilensis </em>and <em>M. platensis</em> in the southern end of South America. Bayesian, non-Bayesian clustering and re-assignment algorithms showed that the natural hybrid zone between <em>M. chilensis </em>and <em>M. platensis </em>in the Strait of Magellan, Isla Grande de Tierra del Fuego, and the Falkland Islands shows complex architecture. It can be divided into three different areas: the first one is on the Atlantic coast where only pure <em>M. platensis</em> and hybrid were found. In the second one, inside the Strait of Magellan, pure individuals of both species and mussels with variable degrees of hybridization coexist. In the last area at the Strait in front of Punta Arenas City, fjords on the Isla Grande de Tierra del Fuego, and at the Beagle Channel, only <em>M. chilensis</em> and a low number of hybrids were found. According to the proportion of hybrids, bays with protected conditions away from strong currents would give better conditions for hybridization. We do not find evidence of any other mussel species such as <em>M. edulis, M. galloprovincialis, M. planulatus, </em>or <em>M. trossulus </em>in the zone</p><p>Funding provided by: Agencia Nacional de Investigación y Desarrollo<br>Crossref Funder Registry ID: https://ror.org/02ap3w078<br>Award Number: Fondecyt Regular 1191765</p><p>Funding provided by: Agencia Nacional de Investigación y Desarrollo<br>Crossref Funder Registry ID: https://ror.org/02ap3w078<br>Award Number: REDES 180143</p><p class="MsoNormal">Mussels (n = 160) from ten sampling sites in the Strait of Magellan, the Isla Grande de Tierra del Fuego, the Falkland Islands, and the Beagle Channel were collected between 2016 and 2018. The DNA was extracted from the mantle edge tissue by the phenol-chloroform method modified for mussels. All individuals were genotyped with a panel of 54 SNPs that have shown good genotyping performance and reproducibility using the Sequenom MassARRAY iPLEX genotyping platform. Individuals with a genotyping rate of less than 90% were excluded from further analysis.</p>
Utility of five SSR markers for genetic diversity and paternity exclusion analysis in the patagonian toothfish
© 2017, Escuela de Ciencias del Mar. All Right Reserved.The Patagonian toothfish or Chilean sea bass (Dissostichus eleginoides), found in the Southern Ocean surrounding Antarctica, is an important fishery species for Chile. This high-value species is regarded as overfished, making it an attractive target for aquaculture. When developing a reproduction program for any aquaculture species, it is important to implement genetic tools to evaluate diversity, inbreeding, and parentage. We calculated genetic diversity and paternity/maternity exclusion probabilities based on five commonly-used microsatellite loci in a natural population of Patagonian toothfish from southern Chile (n = 34) in order to evaluate the potential utility of these five markers in stock management. The observed number of alleles per locus (Na) and observed heterozygosities (HO) are within range as described by studies performed in other sub-Antarctic regions. All five loci were strongly polymorphic, with HO > 0.6 and N
Heterologous microsatellite-based genetic diversity in blue mussel (Mytilus chilensis) and differentiation among localities in southern Chile
Mussels (Mytilus spp.) are one of the most cultivated and commercialized bivalves in southern Chile; culture is currently supplied almost entirely from wild-caught seed obtained from relatively few collection centers. The genetic diversity and differentiation of the blue mussel in southern Chile was investigated by sampling six locations: one natural bank and five seed collection centers. Nine polymorphic microsatellite (SSR) loci were genotyped (Mgu1, Mgu3, MT203, MT282, Mg15, Mg56, Med737, MIT02 and MGE005). We found 75 different alleles, six of which were private alleles. Of the analyzed loci, 45 of 54 tests performed deviated from Hardy-Weinberg equilibrium after sequential Bonferroni correction (P < 0.05), revealing significant heterozygote deficiencies. The polymorphic information content (PIC) ranged from 0.322 (MGE005) to 0.893 (Mgu1). Despite the long distance between some sampling sites (up to 1360 km), genetic differentiation among the sites was low (F ST = 0.043, P < 0.0001). The Bayesian cluster analysis (STRUCTURE) indicated two probable clusters, while the non-parametric cluster analysis (AWclust) identified two to four clusters. Both analyses showed a high level of admixture within clusters. Our results indicate that blue mussels in southern Chile show lower genetic diversity than in other countries, low inbreeding levels, and limited genetic differentiation among locations
Intestinal Transcriptome Analysis Reveals Enrichment of Genes Associated with Immune and Lipid Mechanisms, Favoring Soybean Meal Tolerance in High-Growth Zebrafish (Danio Rerio)
The molecular mechanisms underlying fish tolerance to soybean meal (SBM) remain unclear. Identifying these mechanisms would be beneficial, as this trait favors growth. Two fish replicates from 19 experimental families were fed fishmeal-(100FM) or SBM-based diets supplemented with saponin (50SBM + 2SPN) from juvenile to adult stages. Individuals were selected from families with a genotype-by-environment interaction higher (HG-50SBM + 2SPN, 170 +/- 18 mg) or lower (LG-50SBM + 2SPN, 76 +/- 10 mg) weight gain on 50SBM + 2SPN for intestinal transcriptomic analysis. A histological evaluation confirmed middle intestinal inflammation in the LG- vs. HG-50SBM + 2SPN group. Enrichment analysis of 665 differentially expressed genes (DEGs) identified pathways associated with immunity and lipid metabolism. Genes linked to intestinal immunity were downregulated in HG fish (mpx, cxcr3.2, cftr, irg1l, itln2, sgk1, nup61l, il22), likely dampening inflammatory responses. Conversely, genes involved in retinol signaling were upregulated (rbp4, stra6, nr2f5), potentially favoring growth by suppressing insulin responses. Genes associated with lipid metabolism were upregulated, including key components of the SREBP (mbtps1, elov5l, elov6l) and cholesterol catabolism (cyp46a1), as well as the downregulation of cyp7a1. These results strongly suggest that transcriptomic changes in lipid metabolism mediate SBM tolerance. Genotypic variations in DEGs may become biomarkers for improving early selection of fish tolerant to SMB or others plant-based diets