Photoconversion of Chlorinated Saline Wastewater DBPs in Receiving Seawater is Overall a Detoxification Process

Chlorine disinfection of wastewater effluents rich in bromide and iodide ions results in the formation of relatively toxic bromo- and iodo-disinfection byproducts (DBPs), especially highly toxic bromophenolic and iodophenolic DBPs, which could harm the marine ecosystem when they are discharged into receiving seawater along with the wastewater effluents. In this study, we investigated the conversion of three individual halophenolic DBPs (5-bromosalicylic acid, 2,5-dibromohydroquinone, and 2,4,6-triiodophenol) and two chlorinated saline wastewater DBP mixtures in seawater. The conversion products were analyzed with ultra performance liquid chromatography/electrospray ionization-triple quadrupole mass spectrometry, and the conversion of overall halo-DBPs in the wastewater DBP mixtures was monitored by measuring total organic halogen. The photoconversion-induced variations in the toxicity were evaluated using the embryos of a marine polychaete. Halophenolic DBPs were found to undergo photoconversion in seawater. The conversion was triggered by photonucleophilic substitution: bromophenolic and iodophenolic DBPs were converted to their chlorophenolic or hydroxyphenolic analogues, via substituting the bromine and iodine atoms with chloride or hydroxide ions in seawater; chlorophenolic DBPs were converted to their hydroxyphenolic analogues, via substituting the chlorine atoms with hydroxide ions in seawater. The hydroxyphenolic analogues thus formed further decomposed and finally cleaved to aliphatic compounds. The photoconversion of chlorinated saline wastewater DBPs in receiving seawater was overall a dehalogenation and detoxification process

Comparative Toxicity of Chlorinated Saline and Freshwater Wastewater Effluents to Marine Organisms

Toilet flushing with seawater results in saline wastewater, which may contain approximately 33–50% seawater. Halogenated disinfection byproducts (DBPs), especially brominated and iodinated DBPs, have recently been found in chlorinated saline wastewater effluents. With the occurrence of brominated and iodinated DBPs, the adverse effects of chlorinated saline wastewater effluents to marine ecology have been uncertain. By evaluating the developmental effects in the marine polychaete <i>Platynereis dumerilii</i> directly exposed to chlorinated saline/freshwater wastewater effluents, we found surprisingly that chlorinated saline wastewater effluents were less toxic than a chlorinated freshwater wastewater effluent. This was also witnessed by the marine alga <i>Tetraselmis marina</i>. The toxicity of a chlorinated wastewater effluent to the marine species was dominated by its relatively low salinity compared to the salinity in seawater. The organic matter content in a chlorinated wastewater effluent might be partially responsible for the toxicity. The adverse effects of halogenated DBPs on the marine species were observed pronouncedly only in the “concentrated” chlorinated wastewater effluents. pH and ammonia content in a wastewater effluent caused no adverse effects on the marine species. The results suggest that using seawater to replace freshwater for toilet flushing might mitigate the “direct” acute detrimental effect of wastewater to the marine organisms

Demand-side Regulation Provision of Virtual Power Plants Consisting of Interconnected Microgrids through Double-stage Double-layer Optimization

Demand-side Regulation Provision of Virtual Power Plants Consisting of Interconnected Microgrids through Double-stage Double-layer Optimizatio

Formation of Brominated Disinfection Byproducts during Chloramination of Drinking Water: New Polar Species and Overall Kinetics

The formation of brominated disinfection byproducts (Br-DBPs), which are generally significantly more cytotoxic and genotoxic than their chlorinated analogues, in chloramination has not been fully examined. In this work, the formation of new polar Br-DBPs in simulated drinking waters was examined using state-of-the-art ultraperformance liquid chromatography/electrospray ionization-triple quadrupole mass spectrometry. As many as 29 aliphatic, aromatic, or nitrogenous polar Br-DBPs were detected in chloramination, and five of them (including 2,4,6-tribromoresorcinol, 2,6-dibromo-4-nitrophenol, 2,2,4-tribromo-5-hydroxy-4-cyclopentene-1,3-dione, 2,2,4-dibromochloro-5-hydroxy-4-cyclopentene-1,3-dione, and 2,2,4-bromodichloro-5-hydroxy-4-cyclopentene-1,3-dione) were tentatively identified. Unlike chlorination, chloramination favored the formation of aromatic and nitrogenous polar Br-DBPs and was mild enough to allow polar intermediate Br-DBPs to accumulate. To further explore the formation mechanism of Br-DBPs in chloramination, a quantitative empirical model involving 33 major reactions was developed to describe the overall kinetics. According to the modeling results, bromochloramine and monobromamine were the major species responsible for 54.2–58.1% and 41.7–45.7%, respectively, of the formed Br-DBPs, while hypobromous acid accounted for only 0.2% of the formed Br-DBPs; direct reactions between monochloramine and natural organic matter accounted for the majority of the formed chlorinated DBPs (93.7–95.1%); hypochlorous acid and hypobromous acid in the chloramination were at ng/L or subng/L levels, which were not enough to cause polar intermediate Br-DBPs to decompose

Liquid Crystalline Behavior of Graphene Oxide in the Formation and Deformation of Tough Nanocomposite Hydrogels

In this paper, we report the formation and transformation of graphene oxide (GO) liquid crystalline (LC) structures in the synthesis and deformation of tough GO nanocomposite hydrogels. GO aqueous dispersions form a nematic LC phase, while the addition of poly­(<i>N</i>-vinylpyrrolidone) (PVP) and acrylamide (AAm), which are capable of forming hydrogen bonding with GO nanosheets, shifts the isotropic/nematic transition to a lower volume fraction of GO and enhances the formation of nematic droplets. During the gelation process, a phase separation of the polymers and GO nanosheets is accompanied by the directional assembly of GO nanosheets, forming large LC tactoids with a radial GO configuration. The shape of the large tactoids evolves from a sphere to a toroid as the tactoids increase in size. Interestingly, during cyclic uniaxial tensile deformation a reversible LC transition is observed in the very tough hydrogels. The isolated birefringent domains and the LC domains in the tactoids in the gels are highly oriented under a high tensile strain

Synthesis of Graphene Peroxide and Its Application in Fabricating Super Extensible and Highly Resilient Nanocomposite Hydrogels

Functionalized graphene has been considered as one of the most important materials for preparing polymer nanocomposites due to its unique physical structure and properties. To increase the interfacial interaction between polymer component and graphene oxide (GO) sheets, <i>in situ</i> grafting polymerization initiated by a free radical initiator immobilized on GO sheets is a better choice. We report a facile and effective strategy for preparing graphene peroxide (GPO) <i>via</i> the radiation-induced peroxidation of GO. The formation of peroxides on GO is proven by iodometric measurement and other characterizations. Using GPO as a polyfunctional initiating and cross-linking center, we obtained GO composite hydrogels exhibiting excellent mechanical properties, namely, very high tensile strength (0.2–1.2 MPa), extremely high elongations (2000–5300%), and excellent resilience. This work provides new insight into the fabrication of GO/polymer nanocomposites to fulfill the excellent mechanical properties of graphene

sj-docx-1-hol-10.1177_09596836231197769 – Supplemental material for Holocene summer temperature record based on branched tetraethers in Northeast China

Supplemental material, sj-docx-1-hol-10.1177_09596836231197769 for Holocene summer temperature record based on branched tetraethers in Northeast China by Zeyang Zhu, Jing Wu, Jiaxin Lu, Guoqiang Chu, Patrick Rioual, Luo Wang and Jiaqi Liu in The Holocene</p

Integrating a Biomineralized Nanocluster for H₂S‑Sensitized ROS Bomb against Breast Cancer

Nanomaterial-assisted chemodynamic therapy (CDT) has received considerable attention in recent years. It outperforms other modalities by its distinctive reactive oxygen species (ROS) generation through a nonexogenous stimulant. However, CDT is limited by the insufficient content of endogenous hydrogen peroxide (H2O2). Herein, a biodegradable MnS@HA-DOX nanocluster (MnS@HA-DOX NC) was constructed by in situ biomineralization from hyaluronic acid, to enlarge the ROS cascade and boost Mn2+-based CDT. The acid-responsive NCs could quickly degrade after internalization into endo/lysosomes, releasing Mn2+, H2S gas, and anticancer drug doxorubicin (DOX). The Fenton-like reaction catalyzed by Mn2+ was amplified by both H2S and DOX, producing a mass of cytotoxic ·OH radicals. Through the combined action of gas therapy (GT), CDT, and chemotherapy, oxidative stress would be synergistically enhanced, inducing irreversible DNA damage and cell cycle arrest, eventually resulting in cancer cell apoptosis

MOESM3 of Both HDAC5 and HDAC6 are required for the proliferation and metastasis of melanoma cells

Additional file 3: Figure S3. Knocking down HDAC6 induced apoptosis with time course. Annexin V was used to stain the apoptotic cells and PI was used to stain the dead cells. After constructing knocking down HDAC6 stable cells, we continued to culture these cells in RPMI1640 medium and collected cells with a time course: 1, 3, 5 and 7 days

MOESM2 of Both HDAC5 and HDAC6 are required for the proliferation and metastasis of melanoma cells

Additional file 2: Figure S2. Screening for an efficient shRNA for HDAC5 or HDAC6 knockdown. The seq used for RNA interference are listed in Materials and Methods. HDAC5 (or HADC6) shRNA vectors were transiently transfected in HEK293T cells, and the cells were collected 36 h later, washed twice with ice cold PBS, and centrifuged at 1000 rpm for 5 min. Then, 1 × SDS loading buffer was added and boiled for 10 min; then 10 μl of samples was loaded for SDS-PAGE. β-actin was used as an internal control