MOESM3 of Both HDAC5 and HDAC6 are required for the proliferation and metastasis of melanoma cells

Abstract

Additional file 3: Figure S3. Knocking down HDAC6 induced apoptosis with time course. Annexin V was used to stain the apoptotic cells and PI was used to stain the dead cells. After constructing knocking down HDAC6 stable cells, we continued to culture these cells in RPMI1640 medium and collected cells with a time course: 1, 3, 5 and 7 days