Additional file 2: Figure S2. Screening for an efficient shRNA for HDAC5 or HDAC6 knockdown. The seq used for RNA interference are listed in Materials and Methods. HDAC5 (or HADC6) shRNA vectors were transiently transfected in HEK293T cells, and the cells were collected 36 h later, washed twice with ice cold PBS, and centrifuged at 1000 rpm for 5 min. Then, 1 × SDS loading buffer was added and boiled for 10 min; then 10 μl of samples was loaded for SDS-PAGE. β-actin was used as an internal control
