The impact of the Qinghai-Tibet highway on plant community and diversity

Roads are an increasingly prevalent form of human activity that drives the decrease in plant community functions and threatens global biodiversity. However, few studies have focused on the changes in the function and diversity of alpine meadows caused by road infrastructure in the Tibetan Plateau. In this study, the changes in species diversity, functional diversity, and community stability were examined at different distances from the Qinghai-Tibet highway. The results showed that the road intensified the degradation of vegetation, which significantly altered species diversity and community structure. This effect gradually decreased from near to far from the highway. Plant community cover and species diversity were highest at intermediate distances (50–100 m) from the roadway; species diversity and stability were lowest in the grassland most disturbed by the road (0 m), and species diversity and functional diversity tended to stabilize farther away from the road (250 m). Our findings indicate that changes in species diversity are synchronized with changes in functional diversity, which largely determines the outcome of degraded grassland community diversity and stability. Our results provide a reference point for restoring degraded alpine areas and mitigating the ecological impacts of roads

Effectiveness of 10 polymorphic microsatellite markers for parentage and pedigree analysis in plateau pika (Ochotona curzoniae)

<p>Abstract</p> <p>Background</p> <p>The plateau pika <it>(Ochotona curzoniae) </it>is an underground-dwelling mammal, native to the Tibetan plateau of China. A set of 10 polymorphic microsatellite loci has been developed earlier. Its reliability for parentage assignment has been tested in a plateau pika population. Two family groups with a known pedigree were used to validate the power of this set of markers.</p> <p>Results</p> <p>The error in parentage assignment using a combination of these 10 loci was very low as indicated by their power of discrimination (0.803 - 0.932), power of exclusion (0.351 - 0.887), and an effectiveness of the combined probability of exclusion in parentage assignment of 99.999%.</p> <p>Conclusion</p> <p>All the offspring of a family could be assigned to their biological mother; and their father or relatives could also be identified. This set of markers therefore provides a powerful and efficient tool for parentage assignment and other population analyses in the plateau pika.</p

Investigating the Effectiveness of Road-related Mitigation Measures under Semi-controlled Conditions: A Case Study on Asian Amphibians

Road traffic is the main factor causing the decline in amphibian populations worldwide. The proper design of an amphibian tunnel is one of the most efficient measures to mitigate the negative impacts of road traffic on amphibians. However, no study has investigated the effectiveness of amphibian tunnels under semi-controlled conditions in Asian amphibians. Here, we selected two representative amphibian species, the Chinese brown frog, Rana chensinensis, and the Asiatic toad, Bufo gargarizans, which suffer the most severe road mortality along the roads in Northeast China. We placed experimental arrays of culverts of various sizes (diameters of 1.5, 1, and 0.5 m for circular culverts; side lengths of 1.5, 1, and 0.5 m for box culverts), and substrate type (soil, concrete, and metal) to examine the preferences of both species during the migratory season between May and September in 2016 and 2017. The results revealed that the Chinese brown frog preferred mid- and large-sized culverts as well as soil culverts. We concluded that culverts with a side length ≥ 1 m, lined with soil, and accompanied by a ≥ 0.4 m high guide drift fence and ≤ 45° gradient on the roadside ditch wall would best facilitate road crossings for both species and likely for other amphibian species in Northeast China

Tet2 loss leads to hypermutagenicity in haematopoietic stem/progenitor cells

TET2 is a dioxygenase that catalyses multiple steps of 5-methylcytosine oxidation. Although TET2 mutations frequently occur in various types of haematological malignancies, the mechanism by which they increase risk for these cancers remains poorly understood. Here we show that Tet2?/? mice develop spontaneous myeloid, T- and B-cell malignancies after long latencies. Exome sequencing of Tet2?/? tumours reveals accumulation of numerous mutations, including Apc, Nf1, Flt3, Cbl, Notch1 and Mll2, which are recurrently deleted/mutated in human haematological malignancies. Single-cell-targeted sequencing of wild-type and premalignant Tet2?/? Lin?c-Kit+ cells shows higher mutation frequencies in Tet2?/? cells. We further show that the increased mutational burden is particularly high at genomic sites that gained 5-hydroxymethylcytosine, where TET2 normally binds. Furthermore, TET2-mutated myeloid malignancy patients have significantly more mutational events than patients with wild-type TET2. Thus, Tet2 loss leads to hypermutagenicity in haematopoietic stem/progenitor cells, suggesting a novel TET2 loss-mediated mechanism of haematological malignancy pathogenesis

Tet2 loss leads to hypermutagenicity in haematopoietic stem/progenitor cells

TET2 is a dioxygenase that catalyses multiple steps of 5-methylcytosine oxidation. Although TET2 mutations frequently occur in various types of haematological malignancies, the mechanism by which they increase risk for these cancers remains poorly understood. Here we show that Tet2-/- mice develop spontaneous myeloid, T- and B-cell malignancies after long latencies. Exome sequencing of Tet2-/- tumours reveals accumulation of numerous mutations, including Apc, Nf1, Flt3, Cbl, Notch1 and Mll2, which are recurrently deleted/mutated in human haematological malignancies. Single-cell-targeted sequencing of wild-type and premalignant Tet2-/- Lin-c-Kit+ cells shows higher mutation frequencies in Tet2-/- cells. We further show that the increased mutational burden is particularly high at genomic sites that gained 5-hydroxymethylcytosine, where TET2 normally binds. Furthermore, TET2-mutated myeloid malignancy patients have significantly more mutational events than patients with wild-type TET2. Thus, Tet2 loss leads to hypermutagenicity in haematopoietic stem/progenitor cells, suggesting a novel TET2 loss-mediated mechanism of haematological malignancy pathogenesis

Gut Microbiota in Tibetan Herdsmen Reflects the Degree of Urbanization

Urbanization is associated with shifts in human lifestyles, thus possibly influencing the diversity, interaction and assembly of gut microbiota. However, the question regarding how human gut microbiota adapts to varying lifestyles remains elusive. To understand the relationship between gut microbiota and urbanization, we compared the diversity, interaction and assembly of gut microbial communities of herdsmen from three regions with different levels of urbanization, namely traditional herdsmen (TH), semi-urban herdsmen (SUH) and urban herdsmen (UH). The relative abundance of Prevotella decreased with the degree of urbanization (from TH to UH), whereas that of Bacteroides, Faecalibacterium, and Blautia showed an opposite trend. Although the alpha diversity measures (observed OTUs and phylogenetic diversity) of gut microbiota were unaffected by urbanization, the beta diversity (Jaccard or Bray-Curtis distances) was significantly influenced by urbanization. Metagenome prediction revealed that the gene functions associated with metabolism (i.e., carbohydrate and lipid metabolism) had significant differences between TH and UH. Network analysis showed that the modularity increased with the degree of urbanization, indicating a high extent of niche differentiation in UH. Meanwhile the trend of network density was opposite, indicating a more complex network in TH. Notably, the relative importance of environmental filtering that governed the community assembly increased with the degree of urbanization, which indicated that deterministic factors (e.g , low-fiber diet) play more important roles than stochastic factors (e.g., stochastic dispersal) in shaping the gut microbiota. A quantification of ecological processes showed a stronger signal of variable selection in UH than TH, implying that different selective pressures cause divergent gut community compositions due to urban lifestyles. Our results suggest that beta diversity, network interactions and ecological processes of gut microbiota may reflect the degree of urbanization, and highlight the adaptation of human gut microbiota to lifestyle changes

A System Dynamics Model for Assessing the Efficacy of Lethal Control for Sustainable Management of <i>Ochotona curzoniae</i> on Tibetan Plateau

When population abundances exceed the economical or ecological threshold, animals are always regarded as pests, so effective and sustainable management strategies are required. As a native species widely distributed on Tibetan Plateau, plateau pika is regarded as a pest when its abundance is too high. For decades, plateau pika were controlled using toxic baits by both the local government and shepherds. However, how pika population fluctuates after lethal control is far from certain. Based on our previous studies, demographic parameters of plateau pika were acquired. A system dynamics model is developed to parameterize a population model for this species. The model incorporates two age categories (juvenile and adult) of both sexes, and uses density-dependent factors, including reproduction, mortality, and migration. The model is used originally to analyze the effect of pika management on the population dynamics and resulting abundance, in order to plan optimal pest controlling strategies. The results show that lethal control is efficient when continuously conducted once each year, or twice with 2-year intervals. For sustainable controlling pest abundance, comprehensive strategies should be considered. An appropriate population model could be used to explore the optimal strategies and provide important reference into decision making about pest management

Effects of Heavy Degradation on Alpine Meadows: Soil N2O Emission Rates and Meta-Analysis in the Tibetan Plateau

Heavy grassland degradation is evident across the Tibetan Plateau. However, atmospheric nitrous oxide (N2O) emission rates and their underlying driving mechanisms in the southeast regions and across the Tibetan Plateau remain unclear. We analyzed the N2O emission rates of heavily degraded and undegraded alpine meadow soil incubation using gas chromatography in three river sources and meta-analysis methods across the Tibetan Plateau. The N2O emission rates of the heavily degraded and control meadows were respectively 4.29 &plusmn; 0.64 and 3.27 &plusmn; 0.53 g kg&minus;1 h&minus;1 in the southeast Tibetan Plateau (p &lt; 0.01), indicating an increase of 31.16% on the N2O flux of heavy degradation. Heavy degradation increased N2O emission rates by 0.55 &plusmn; 0.14 (95% confidence interval: 0.27&ndash;0.83) through meta-analysis. High degradation increased by approximately 71.6% compared with that of the control. The water-filled pore space (WFPS) significantly influenced the N2O emission rate based on the moderator test (p &lt; 0.05). The mixed-effect model results revealed that WFPS, soil nitrate, and bulk soil could explain 59.90%, 16.56%, and 15.19% of the variation in the N2O emission rates between the control and heavily degraded meadows, respectively. In addition, the N2O emission rates of heavily degraded meadows can be reduced by increasing WFPS and bulk density, and by reducing the soil nitrate content

Online Extraction of Pose Information of 3D Zigzag-Line Welding Seams for Welding Seam Tracking

Three-dimensional (3D) zigzag-line welding seams are found extensively in the manufacturing of marine engineering equipment, heavy lifting equipment, and logistics transportation equipment. Currently, due to the large amount of calculation and poor real-time performance of 3D welding seam detection algorithms, real-time tracking of 3D zigzag-line welding seams is still a challenge especially in high-speed welding. For the abovementioned problems, we proposed a method for the extraction of the pose information of 3D zigzag-line welding seams based on laser displacement sensing and density-based clustering point cloud segmentation during robotic welding. after thee point cloud data of the 3D zigzag-line welding seams was obtained online by the laser displacement sensor, it was segmented using theρ-Approximate DBSCAN (Density-Based Spatial Clustering of Applications with Noise) algorithm. In the experiment, high-speed welding was performed on typical low-carbon steel 3D zigzag-line welding seams using gas metal arc welding. The results showed that when the welding velocity was 1000 mm/min, the proposed method obtained a welding seam position detection error of less than 0.35 mm, a welding seam attitude estimation error of less than two degrees, and the running time of the main algorithm was within 120 ms. Thus, the online extraction of the pose information of 3D zigzag-line welding seams was achieved and the requirements of welding seam tracking were met