Transcriptome and Comparative Gene Expression Analysis of Sogatella furcifera (Horváth) in Response to Southern Rice Black-Streaked Dwarf Virus

BACKGROUND: The white backed planthopper (WBPH), Sogatella furcifera (Horváth), causes great damage to many crops by direct feeding or transmitting plant viruses. Southern rice black-streaked dwarf virus (SRBSDV), transmitted by WBPH, has become a great threat to rice production in East Asia. METHODOLOGY/PRINCIPAL FINDINGS: By de novo transcriptome assembling and massive parallel pyrosequencing, we constructed two transcriptomes of WBPH and profiled the alternation of gene expression in response to SRBSDV infection in transcriptional level. Over 25 million reads of high-quality DNA sequences and 81388 different unigenes were generated using Illumina technology from both viruliferous and non-viruliferous WBPH. WBPH has a very similar gene ontological distribution to other two closely related rice planthoppers, Nilaparvata lugens and Laodelphax striatellus. 7291 microsatellite loci were also predicted which could be useful for further evolutionary analysis. Furthermore, comparative analysis of the two transcriptomes generated from viruliferous and non-viruliferous WBPH provided a list of candidate transcripts that potentially were elicited as a response to viral infection. Pathway analyses of a subset of these transcripts indicated that SRBSDV infection may perturb primary metabolism and the ubiquitin-proteasome pathways. In addition, 5.5% (181 out of 3315) of the genes in cell cytoskeleton organization pathway showed obvious changes. Our data also demonstrated that SRBSDV infection activated the immunity regulatory systems of WBPH, such as RNA interference, autophagy and antimicrobial peptide production. CONCLUSIONS/SIGNIFICANCE: We employed massively parallel pyrosequencing to collect ESTs from viruliferous and non-viruliferous samples of WBPH. 81388 different unigenes have been obtained. We for the first time described the direct effects of a Reoviridae family plant virus on global gene expression profiles of its insect vector using high-throughput sequencing. Our study will provide a road map for future investigations of the fascinating interactions between Reoviridae viruses and their insect vectors, and provide new strategies for crop protection

Monoclonal antibody-based serological methods for detection of Cucumber green mottle mosaic virus

<p>Abstract</p> <p>Background</p> <p>Cucumber green mottle mosaic virus (CGMMV), a member of the genus <it>Tobamovirus</it>, can be transmitted by seeds and infects many cucurbit species, causing serious yield losses in cucumber and watermelon plants. In this paper, five serological methods including antigen-coated plate enzyme-linked immunosorbent assay (ACP-ELISA), triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), Dot-immunobinding assay (DBIA), direct tissue blot immunoassay (DTBIA) and immunocapture reverse transcriptase polymerase chain reaction (IC-RT-PCR) were described for detection and diagnosis of CGMMV.</p> <p>Results</p> <p>Using the purified CGMMV particles as immunogens, six murine monoclonal antibodies (MAbs) were produced. Five serological methods were established using the MAb 4H1 and detection sensitivity was compared using purified preparations and infected-plant tissue extracts. The detection sensitivity of ACP-ELISA was 0.16 ng of purified CGMMV, whereas TAS-ELISA was more sensitive than ACP-ELISA with a minimum detection of 0.04 ng of purified CGMMV. The sensitivities of TAS-ELISA and DBIA were similar for detecting CGMMV in infected-plant tissue extracts, and were four times higher than ACP-ELISA. The IC-RT-PCR was the most sensitive method, which could detect as little as 0.1 pg of purified virus. The detection sensitivity of IC-RT-PCR for CGMMV-infected plant tissues was about 400 times higher than that of TAS-ELISA and DBIA.</p> <p>Conclusions</p> <p>The established ACP-ELISA, TAS-ELISA, DBIA and DTBIA are suitable for routine CGMMV detection of large-scale samples in the field survey, while IC-RT-PCR is more sensitive and suitable for acquiring information about the viral genome.</p

Dual Immunotherapy in advanced or Metastatic Non-Small Cell Lung Cancer: a Network Meta-Analysis

OBJECTIVES: Recently, there has been extensive research on dual immunotherapy for advanced or metastatic non-small cell lung cancer (NSCLC), yet a comprehensive evaluation is lacking. This study aimed to rank the available treatment options and assess the efficacy and safety of dual immunotherapy regimens through the implementation of a Bayesian network meta-analysis (NMA). MATERIALS AND METHODS: A thorough search was conducted to recognize eligible randomized controlled trials (RCTs) on March 20, 2023. Overall survival (OS), progression-free survival (PFS), treatment-related adverse events (TRAEs) and grade ≥3 TRAEs were evaluated to identify the efficacy and safety of dual immunotherapy regimens. The surface under the cumulative ranking curve (SUCRA) and RESULTS: Eleven clinical trials involving six different regimens were included in this study. The combination of anti-programmed cell death protein 1/programmed cell death ligand 1 (PD-1/PD-L1) antibodies with anti-T-cell immunoglobulin and ITIM domain (TIGIT) antibodies emerged as the most promising regimen for improving OS and PFS, followed by anti-PD-1/PD-L1 + anti-cytotoxic T lymphocyte antigen 4 (CTLA-4) + chemotherapy treatment and anti-PD-1/PD-L1 + anti-CTLA-4 treatment. The forest plots demonstrated that these three regimens were all superior to chemotherapy. The above results were observed in both unselected treatment line and first-line settings. The least likely to be associated with TRAEs and grade ≥3 TRAEs were respectively anti-CTLA-4 treatment and anti-PD-1/PD-L1 + anti-TIGIT treatment, with anti-PD-1/PD-L1 + anti-CTLA-4 + chemotherapy treatment to be the worst. CONCLUSIONS: This NMA validated the promising efficacy and safety of dual immunotherapy in advanced or metastatic NSCLC. Among them, anti-PD-1/PD-L1 + anti-TIGIT regimen emerges as a highly potential therapeutic approach. Ongoing research efforts should focus on improving treatment regimens, identifying biomarkers, and managing TRAEs to optimize the patient benefits of dual immunotherapy

The transition from incoherent to coherent random laser in defect waveguide based on organic/inorganic hybrid laser dye

This paper systematically demonstrated a variety of experimental phenomena of random lasers (RLs) of N,N′-di-(3-(isobutyl polyhedral oligomeric silsesquioxanes)propyl) perylene diimide (DPP) organic/inorganic hybrid laser dye, which is composed of perylene diimide (PDI) as gain media and polyhedral oligomeric silsesquioxanes (POSS) as scattering media at a mole ratio of 1:2. In this work, we observe the transition from incoherent RL in the DPP-doped solutions and polymer membrane systems using dip-coating method to coherent RL in the polymer membrane system with defect waveguide using semi-polymerization (SP) coating method. Meanwhile, we found that the hybrid dye-DPP has a long lasing lifetime compared with the traditional laser dyes, which indicates that the POSS group can suppress the photo-bleaching effect to extend the working life of laser dyes

Power optimization control of VSC-HVDC system for electromechanical oscillation suppression and grid frequency control

The voltage source converter (VSC) based high-voltage DC (HVDC) transmission system usually adopts damping and inertia control to quickly and independently adjust the active- and reactive- power, to improve the frequency stability and suppress the electromechanical oscillations of the power grid. This paper first analyzes the effect of the proportional-derivative (PD) controller parameter on the HVDC output power. The study shows that when the proportional-derivative controller parameter is increased to the limit value, HVDC will operate in the rapid power compensation (RPC) mode. Namely, according to the positive or negative polarities of the rotor speed deviation and the grid frequency deviation, the active- and reactive- power limits are used as the reference to rapidly control the output power, thereby minimizing the system’s unbalanced power, the rotor oscillation, and the frequency fluctuation. To this end, this paper proposes a coordinated active-/reactive- power control strategy for the VSC-HVDC system based on the RPC mode to suppress the grid electromechanical and frequency oscillations. The RPC mode enables HVDC to quickly release/absorb power, to compensate for system’s required power shortage or suppress excess power. When the speed deviation, the frequency deviation, and their rates of change meet the requirements, the damping control is used to make HVDC exit the RPC mode and further enhance the ability of the VSC-HVDC system. Simulation results prove the effectiveness of the proposed power optimization control strategy

Orbital-angular-momentum fluorescence emission based on photon–electron interaction in a vortex field of an active optical fiber

We develop a model of interaction between photons and electrons in an active vortex field, which can generate a fluorescence spectrum with the characteristics of orbital angular momentum (OAM). In an active optical fiber, our findings generalize the notion of photon–electron interaction and point to a new kind of OAM-mode broad-spectrum light source, which could be interpreted in two processes: one microscopically is the excitation of OAM-carrying photons based on the photon–electron interaction; the other macroscopically is the emission and transmission of a donut-shaped fluorescence in a vortex field with a spiral phase wavefront in a ring-core active fiber. Here we present a straightforward experimental method that the emission of broad-spectrum fluorescence with an OAM feature is actualized and validated in a ring-core erbium-doped fiber. The spectrum has a broad spectral width up to 50 nm. Furthermore, four wavelengths are extracted from the fluorescence spectrum and superimposed with their corresponding Gaussian beams, from which the spiral-shaped interferograms of OAM modes in a broad spectrum are identified with high purity. The application of the OAM-based fluorescence light source may range from classical to quantum information technologies, and enable high-capacity communication, high-sensitivity sensing, high-resolution fluorescence imaging, etc

Salp swarm optimization algorithm based MPPT design for PV-TEG hybrid system under partial shading conditions

This paper proposes an innovative strategy to integrate thermoelectric generator (TEG) and photovoltaic (PV) systems, aiming to enhance energy production efficiency by addressing the significant waste heat generated during traditional PV system operation. Additionally, photovoltaic-thermoelectric generator (PV-TEG) hybrid system encounters the dual challenge of partial shading conditions (PSC) and non-uniform temperature distribution (NTD). Thus, salp swarm optimization (SSA) is introduced to simultaneously tackle the negative impacts of PSC and NTD. In contrast to alternative meta-heuristic algorithms (MhAs) and conventional mathematical approaches, the streamlined and effective optimization mechanism inherent to SSA affords a shorter optimization time, while mitigating the risk of the PV-TEG hybrid system's optimization outcomes being confined to local maximum power points (LMPP). Furthermore, the optimization performance of SSA for PV-TEG hybrid systems is assessed via four case studies, including start-up test, stepwise variations in solar irradiation at constant temperature, stochastic change in solar irradiation, and field measured data for typical days in Hong Kong, in which simulation results show that SSA evinces unparalleled global exploration and local search capabilities, yielding heightened energy output (up to 43.75%) and effectively suppressing power fluctuations in the PV-TEG hybrid system (as evidenced by ΔVavg and ΔVmax)

Low-noise-figure and high-purity 10 vortex modes amplifier based on configurable pump modes

We have explored an orbital angular momentum (OAM) amplifier of 10 vortex modes under different-order OAM pump modes, i.e. OAM0, OAM1, and OAM2. The all-fiber amplification system consists of an active few-mode erbium-doped fiber (FM-EDF), a mode selective pump (MSP), and a mode selective signal (MSS). These mode selective components are based on fused-taper mode selective couplers (MSC) under different wavelengths fabricated by a passive ring-core fiber (RCF). Under different-order mode pumps, the OAM amplifier experimentally exhibits mode gains (MGs) above 15 dB for 10 vortex modes with the mode purities only 89%, essentially in line with the simulation results. Especially when the signal-mode profiles are better matched to the pump-mode profiles, i.e. the OAM pumps with the same order as signals, the obtained MGs are all over 20.2 dB and the amplified OAM mode purity is up to 97%; the acquired noise figures (NFs) are <4.9 dB and even the minimum NF is 3.2 dB. The results reveal that the OAM amplifier shows low-NF and high-purity characteristics under configurable pump modes in C-band. The amplified high-order OAM mode could be promising for uses in the long-distance mode division multiplexing (MDM) and in mitigation of the upcoming capacity crunch in optical fiber communication