Efficient Dihydroxylation of Naphthalene on Photoirradiated Rutile TiO2 Powder in Solution Containing Hydrogen Peroxide

We report on enhanced dihydroxylation of naphthalene on photoirradiated TiO2 particles by addition of H2O2 to the reaction solution. The rate was enhanced by 6–40 times by the addition of H2O2, and the quantum yield reached as high as 76% for rutile TiO2 powders. On the other hand, no such enhancement was observed for anatase TiO2 powders

DIFFERENT CONCENTRATIONS OF SIJUNZI DECOCTION INHIBIT PROLIFERATION AND INDUCE APOPTOSIS OF HUMAN GASTRIC CANCER SGC-7901 SIDE POPULATION

Background: SD is a traditional Chinese medicine which composed of Ginseng, Atractylodes, Poria and Licorice. It is one of the commonly used Chinese traditional medicines that showed anti-gastric cancer activity in clinical studies. Previous evidence demonstrated SD parties (Ginseng, Atractylodes, Poria, Licorice) can inhibit proliferation and induced apoptosis for gastric cancer cell. In order to further investigate the anticancer effect of SD in gastric cancer, we observed the effects of different concentrations of SD on proliferation and apoptosis of SP of human gastric cancer SGC-7901. Materials and Methods: 1. SGC-7901 side population cells were sorted through flow cytometry. 2. To detect the changes of proliferation of SP and NSP before and after the intervention of serum containing different concentrations of SD using cck-8 method. 3. To detect the changes of cell cycle and apoptosis of SP and NSP before and after the intervention of serum containing different concentrations of SD through flow cytometry. 4. To detect the effects of serum containing different concentrations of SD on apoptosis-related proteins Bax and Bcl-2 of SP and NSP before and after the intervention by western-blot. Results: It was found that different concentrations of SD serum treatments inhibited cell proliferation in a time-dependent and concentration-dependent manner. Compared with the control group (normal saline treatment), there were increase in G1/G0 phase population of SP and NSP, and decrease in G2/M and S phase population (

SIJUNZI DECOCTION DEMOLITION PARTIES INHIBIT PROLIFERATION AND INDUCE APOPTOSIS OF HUMAN GASTRIC CANCER BGC823 SIDE POPULATION

Background: Comprehensive treatment combining with Chinese medicine has become the main therapeutic regimen of gastric cancer. Previous evidence demonstrated SD can enhance the effect of chemotherapy in advanced cancer, especially in gastric cancer. In order to investigate the anticancer mechanism of SD in gastric cancer, we observed the effects of SD parties (Ginseng, Atractylodes, Poria, Licorice) on proliferation and apoptosis of SP of human gastric cancer BGC-823. Materials and Methods: 1. BGC-823 side population cells were sorted through flow cytometry. 2. To detect the changes of proliferation of SP and NSP before and after the intervention of serum containing SD parties using cck-8 method. 3. To detect the changes of cell cycle and apoptosis of SP and NSP before and after the intervention of serum containing SD parties through flow cytometry. 4. To detect the effects of serum containing SD parties on apoptosis-related proteins Bax and Bcl-2 of SP and NSP before and after the intervention by western-blot. Results: It was found that four demolition parties serum treatments inhibited cell proliferation in a time-dependent manner. Compared with the control group (normal saline treatment), there were increase in G1/G0 phase population of SP and NSP, and decrease in G2/M and S phase population (

Forskolin reduces fat accumulation in Nile tilapia (Oreochromis niloticus) through stimulating lipolysis and beta-oxidation

© 2018 Published by Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license: http://creativecommons.org/licenses/by-nc-nd/4.0/ This author accepted manuscript is made available following 12 month embargo from date of publication (December 2018) in accordance with the publisher’s archiving policyHigh fat diets are commonly used in aquaculture to reduce feed cost in Nile tilapia, but impair its lipid homeostasis. This study evaluated the role of forskolin on reducing fat accumulation in Nile tilapia (Oreochromis niloticus) by using in vitro and in vivo experiments. The use of 50 μM forskolin in vitro increased free fatty acid and glycerol release, but decreased triglyceride in adipocytes and hepatocytes. The adipose triglyceride lipase (ATGL), protein kinase cAMP-dependent type I regulatory subunit alpha (PKAR I) and other genes related to β-oxidation (peroxisome proliferator activated receptor alpha, PPARα and carnitine O-palmitoyltransferase 1, CPT1) were significantly up-regulated. After feeding a high-fat diet for six weeks, O. niloticus were fed with 0 (control), 0.5 and 1.5 mg/kg forskolin for two weeks to determine whether forskolin could reduce fat accumulation in vivo. Fish fed the two levels of forskolin decreased significantly the hepatosomatic and mesenteric fat indices. The total lipid in the whole fish and liver together with the serum glycerol content were lower in fish fed on forskolin than in the control. The fish fed on forskolin diets exhibited smaller areas of lipid droplets in adipose and liver tissues. Lipolysis related genes (ATGL, hormone-sensitive lipase, HSL; monoacylglycerol lipase, MGL; and protein kinase cAMP-activated catalytic subunit, PKAC) and β-oxidation genes (PPARα; fatty acid binding protein 1, FABP1; and CPT1) in the adipose were up-regulated. Similarly, in the liver lipolysis genes such as ATGL and PKAR I and β-oxidation genes (PPARα, FABP1, CPT1 and acyl-CoA oxidase, ACO) showed an increasing trend with the increase of forskolin doses. This study indicates that forskolin can reduce fat accumulation in the adipose and liver by stimulating lipolysis and β-oxidation in O. niloticus

Spondin-2 (SPON2), a More Prostate-Cancer-Specific Diagnostic Biomarker

BACKGROUND: Prostate-specific antigen (PSA) screening, although common, has recently been called into question. To find prostate cancer (PCa) diagnostic biomarkers that can make up for the defects of PSA, we compared the secretomes of several benign and PCa cell lines, selected candidate molecules, and then confirmed their clinical value. METHODOLOGY/PRINCIPAL FINDINGS: We first identified extracellular proteins by two-dimensional gel electrophoresis (2-DE) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) identification. We then validated the secreted proteins on a cellular level, and finally determined whether they could be used as PCa diagnostic biomarkers using prostate tissue and serum specimens of Chinese volunteers by immunohistostaining and sandwich ELISA. We obtained credible extracellular protein 2-DE graphs of prostate cell lines. The 5 spots that showed superior repeatability were selected for LC-MS/MS analysis, which identified seven candidate molecules. One of the candidate molecules, spondin-2 (SPON2), was only expressed in the conditioned media (CM) of androgen receptor (AR) positive PCa cell lines. Using tissue microarray by immunohistostaining, we found SPON2 to be over-expressed in PCa. SPON2 staining was more intense in Gleason score sum 7-8 and in PCa patients with metastasis. By receiver operator characteristic (ROC) curve analysis, we found that the serum SPON2 level was elevated in PCa patients, showing sensitivity and specificity suitable for diagnostic use. We also found that SPON2 could be used to identify PCa patients with serum PSA levels no higher than 10 ng/ml from healthy elderly men. CONCLUSION/SIGNIFICANCE: SPON2 is a new serum and histological diagnostic biomarker for PCa. It can avoid some of the problems of PSA testing and was here found to offer relatively high sensitivity and specificity relative to PSA

A High-Performance Transmitarray Antenna with Thin Metasurface for 5G Communication Based on PSO (Particle Swarm Optimization)

A 5G metasurface (MS) transmitarray (TA) feed by compact-antenna array with the performance of high gain and side-lobe level (SLL) reduction is presented. The proposed MS has two identical metallic layers etched on both sides of the dielectric substrate and four fixed vias connecting two metallic layers that works at 28 GHz to increase the transmission phase shift range. The proposed planar TA consisting of unit cells with different dimensional information can simulate the function as an optical lens according to the Fermat’s principle, so the quasi-spherical wave emitted by the compact Potter horn antenna at the virtual focal point will transform to the quasi-plane wave by the phase-adjustments. Then, the particle swarm optimization (PSO) is introduced to optimize the phase distribution on the TA to decrease the SLL further. It is found that the optimized TA could achieve 27 dB gain at 28 GHz, 11.8% 3 dB gain bandwidth, −30 dB SLL, and aperture efficiency of 23% at the operating bandwidth of 27.5–29.5 GHz, which performs better than the nonoptimized one. The advanced particularities of this optimized TA including low cost, low profile, and easy to configure make it great potential in paving the way to 5G communication and radar system

Study on the Influence of Clogging on the Cooling Performance of Permeable Pavement

Permeable pavement is often known as “cool road”. However, the cooling performance will be weakened due to clogging. In this paper, the temperature field distribution model of asphalt pavement was obtained by Green’s function. Gradations of porous asphalt mixture were designed to obtain different porosities, and the thermal properties of specimens with different porosities were tested and calculated. The simulation test was carried out to obtain the heating curves, which were used to verify the accuracy of the temperature model by comparing the results of the theoretical calculation to results of the test. The daily solar radiation intensity and air temperature changing functions were plugged into the model to calculate the temperature at the bottom of the middle surface. In this way, the simplified model of void fraction and cooling performance of the porous asphalt pavement was obtained. The results showed that the temperature at the bottom of the middle surface for permeable pavement was lower than that for traditional asphalt pavements. The gap was between 0.29 to 2.75 °C and it increased as the porosity of permeable pavement increased